BobuA.01031.a

glucose-6-phosphate 1-dehydrogenase (BB_0636)

CENTER ID: BobuA.01031.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01031.a.B1.GE40451 Full length( BobuA.01031.a ) 1 478
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127121
PATRIC ID: fig|224326.49.peg.1027
RefSeq: NP_212770.1
UniProt: O51581
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKERSVSNFD IVIFGVTGNL SRKKLIPSLF NLFKNKCISN FRVIGFSRKI FTDKEFRLYI KDSLWQEETD SLIEIFLNFF VYVFGDFNEK ESYKNLFKFL DRSRETIYYL STSPAFYGPI INHLKKYFLS EKLTLSKIVL EKPFGSSLET AKKLNSLLYS AFKEDQIYRI DHYLGKETVQ NIFTFRFGNS IFENIWNNRY VDFVQITVAE ELGLDGRVEY YDSVGALKDM VQNHILQLLS LVAMESPIKF DSEFIHDEKV KVLKSLRKIS KEDIKNYIVK GQYIGSQVQG VFKKGYKDET EFLGNSNTET YLAMKVFINN WRWSGVPFYL RTGKGLARKF SEIYIQFKKP SFTLFNNSSV DFSNALIFRI QPRDGIEIKF NTKKPGYNYE IQTANMEFSY HGAFKRLFDE AYERLLLDAF LGDGTLYATS DEIESSWEFV SDIANKWADI EICNYFYGSE GPKEIDSILE KDHFWRKI
NT Sequence
ATGAAAGAAA GAAGTGTTTC TAATTTTGAT ATTGTAATTT TTGGGGTTAC CGGGAATTTG TCTAGAAAAA AGCTTATTCC TTCACTTTTT AATTTATTTA AAAATAAATG TATTAGCAAT TTTAGGGTTA TTGGTTTTTC TCGTAAGATT TTTACAGATA AAGAATTTAG ATTGTATATT AAAGATTCTT TATGGCAGGA AGAGACCGAT TCGTTGATTG AGATTTTTTT AAATTTTTTT GTTTATGTAT TTGGCGATTT TAATGAAAAG GAGTCTTATA AAAATTTATT TAAATTTTTA GATAGAAGTC GAGAAACGAT ATATTATCTT TCGACGTCTC CTGCATTTTA TGGACCTATA ATTAATCATT TGAAAAAGTA TTTTTTAAGC GAAAAATTGA CTTTGTCAAA AATAGTTCTT GAGAAGCCTT TTGGCTCTAG TCTTGAGACA GCAAAAAAAT TAAATAGTTT GCTTTATTCT GCTTTTAAAG AAGATCAAAT TTATAGAATA GATCACTATT TGGGTAAAGA AACGGTTCAA AATATTTTTA CATTTAGATT CGGCAATTCT ATTTTTGAAA ATATTTGGAA TAATCGTTAT GTAGATTTTG TTCAGATTAC GGTAGCAGAA GAATTAGGTC TTGATGGAAG AGTAGAGTAT TACGATTCTG TTGGCGCTTT GAAGGATATG GTTCAAAATC ATATTTTACA ATTGTTAAGC CTTGTTGCAA TGGAGTCTCC TATTAAATTT GATTCTGAGT TTATTCATGA TGAAAAAGTA AAAGTTTTAA AAAGTTTGAG GAAAATTAGC AAAGAAGATA TTAAGAATTA CATTGTTAAG GGTCAATATA TAGGCTCACA AGTTCAAGGG GTTTTTAAAA AAGGCTATAA AGATGAAACA GAATTTTTGG GAAATTCAAA TACCGAAACT TATTTAGCTA TGAAAGTGTT TATTAATAAT TGGCGTTGGT CTGGGGTTCC TTTTTATCTT AGAACTGGGA AAGGTCTTGC TAGGAAATTT TCAGAAATAT ATATTCAATT TAAAAAACCG AGCTTTACTC TTTTTAACAA TAGTTCTGTT GATTTTTCTA ATGCTTTGAT ATTTAGAATT CAGCCAAGGG ATGGAATTGA AATCAAATTC AATACCAAGA AGCCCGGATA TAATTATGAA ATTCAAACTG CTAATATGGA GTTTTCATAT CACGGAGCAT TTAAAAGATT GTTTGATGAG GCTTATGAGC GTTTATTGTT AGACGCTTTT TTAGGAGATG GTACTTTATA TGCGACAAGT GATGAGATTG AAAGTTCTTG GGAATTTGTT TCAGATATTG CAAATAAATG GGCAGATATT GAAATTTGTA ATTATTTTTA TGGCTCTGAA GGACCAAAAG AGATAGACTC TATTTTAGAA AAAGATCATT TTTGGCGTAA AATT
Details for BobuA.01031.a.B1.GE40451
HARVESTED ON: 8/10/2016
SEQUENCED ON: 8/11/2016
EXPECTED MW: 57kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK ERSVSNFDIV IFGVTGNLSR KKLIPSLFNL FKNKCISNFR VIGFSRKIFT DKEFRLYIKD SLWQEETDSL IEIFLNFFVY VFGDFNEKES YKNLFKFLDR SRETIYYLST SPAFYGPIIN HLKKYFLSEK LTLSKIVLEK PFGSSLETAK KLNSLLYSAF KEDQIYRIDH YLGKETVQNI FTFRFGNSIF ENIWNNRYVD FVQITVAEEL GLDGRVEYYD SVGALKDMVQ NHILQLLSLV AMESPIKFDS EFIHDEKVKV LKSLRKISKE DIKNYIVKGQ YIGSQVQGVF KKGYKDETEF LGNSNTETYL AMKVFINNWR WSGVPFYLRT GKGLARKFSE IYIQFKKPSF TLFNNSSVDF SNALIFRIQP RDGIEIKFNT KKPGYNYEIQ TANMEFSYHG AFKRLFDEAY ERLLLDAFLG DGTLYATSDE IESSWEFVSD IANKWADIEI CNYFYGSEGP KEIDSILEKD HFWRKI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aaagaagtgt ttctaatttt gatattgtaa tttttggggt taccgggaat ttgtctagaa aaaagcttat tccttcactt tttaatttat ttaaaaataa atgtattagc aattttaggg ttattggttt ttctcgtaag atttttacag ataaagaatt tagattgtat attaaagatt ctttatggca ggaagagacc gattcgttga ttgagatttt tttaaatttt tttgtttatg tatttggcga ttttaatgaa aaggagtctt ataaaaattt atttaaattt ttagatagaa gtcgagaaac gatatattat ctttcgacgt ctcctgcatt ttatggacct ataattaatc atttgaaaaa gtatttttta agcgaaaaat tgactttgtc aaaaatagtt cttgagaagc cttttggctc tagtcttgag acagcaaaaa aattaaatag tttgctttat tctgctttta aagaagatca aatttataga atagatcact atttgggtaa agaaacggtt caaaatattt ttacatttag attcggcaat tctatttttg aaaatatttg gaataatcgt tatgtagatt ttgttcagat tacggtagca gaagaattag gtcttgatgg aagagtagag tattacgatt ctgttggcgc tttgaaggat atggttcaaa atcatatttt acaattgtta agccttgttg caatggagtc tcctattaaa tttgattctg agtttattca tgatgaaaaa gtaaaagttt taaaaagttt gaggaaaatt agcaaagaag atattaagaa ttacattgtt aagggtcaat atataggctc acaagttcaa ggggttttta aaaaaggcta taaagatgaa acagaatttt tgggaaattc aaataccgaa acttatttag ctatgaaagt gtttattaat aattggcgtt ggtctggggt tcctttttat cttagaactg ggaaaggtct tgctaggaaa ttttcagaaa tatatattca atttaaaaaa ccgagcttta ctctttttaa caatagttct gttgattttt ctaatgcttt gatatttaga attcagccaa gggatggaat tgaaatcaaa ttcaatacca agaagcccgg atataattat gaaattcaaa ctgctaatat ggagttttca tatcacggag catttaaaag attgtttgat gaggcttatg agcgtttatt gttagacgct tttttaggag atggtacttt atatgcgaca agtgatgaga ttgaaagttc ttgggaattt gtttcagata ttgcaaataa atgggcagat attgaaattt gtaattattt ttatggctct gaaggaccaa aagagataga ctctatttta gaaaaagatc atttttggcg taaaatttaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat