BobuA.01025.a

oligopeptide ABC transporter, ATP-binding protein (oppD) (BB_0334)

CENTER ID: BobuA.01025.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01025.a.A1.GE26473 Full length( BobuA.01025.a ) 1 290
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.01025.a.A1.PS00429 Full length( BobuA.01025.a ) 1 290

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_128716
PATRIC ID: fig|224326.49.peg.730
RefSeq: NP_212468.1
UniProt: O31309

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKENILEIK NLTIEFRLKH TTIHPVSNVN LSVKRGEIRA IVGESGSGKS VTSMAILKLL PELTTVYKSG EILFENQDLL KLSEKELLKI RGNKISMIFQ DPMTSLNPFL RISTQLEETI ILHQGLGKKE AKEKAIEMLK TVGVVNAEER IKHFPHQFSG GMRQRVMIAM ALSCHPSLLI ADEPTTALDV TIQEQILLLI KNLSKKFNTS TIFITHDLAV VAEICDTVSV MYQGKIVEEG TVEEIFNNPK HPYTIGLLKS ILTLEHDPNK KLYSTKENPM KITKTSTEEF
NT Sequence
ATGGAAAAAG AAAATATATT GGAAATAAAA AATTTAACAA TTGAATTTAG ATTAAAACAT ACAACAATTC ATCCCGTAAG CAATGTTAAC CTATCTGTAA AAAGAGGAGA AATTAGAGCT ATTGTTGGAG AATCTGGAAG CGGAAAATCC GTAACAAGTA TGGCTATTTT AAAATTATTA CCAGAACTTA CAACAGTATA TAAAAGTGGA GAAATACTAT TTGAAAATCA AGATCTGCTA AAACTTAGCG AAAAAGAACT TTTAAAAATC AGAGGGAATA AAATATCAAT GATATTTCAA GACCCAATGA CTTCATTAAA CCCATTTTTA AGAATATCAA CTCAACTTGA AGAAACAATA ATCTTACACC AAGGATTAGG GAAAAAAGAA GCCAAAGAAA AAGCAATAGA AATGTTAAAA ACTGTTGGTG TTGTAAACGC AGAAGAAAGA ATAAAACATT TCCCACATCA ATTTTCAGGA GGAATGAGAC AAAGAGTTAT GATTGCCATG GCTCTTAGCT GTCATCCATC CTTATTAATA GCAGATGAAC CAACAACAGC CCTTGATGTT ACAATCCAAG AGCAAATATT ATTATTAATC AAAAACCTAT CTAAAAAATT CAATACTTCT ACCATATTTA TAACTCATGA TCTTGCGGTT GTTGCTGAAA TTTGTGATAC AGTATCTGTA ATGTATCAAG GAAAAATTGT AGAAGAAGGA ACAGTAGAGG AAATATTTAA CAATCCTAAG CATCCTTACA CCATTGGGCT TTTAAAATCA ATTCTTACGC TAGAACACGA TCCAAATAAA AAGCTTTATT CAACAAAAGA AAACCCTATG AAGATCACAA AAACCAGCAC CGAGGAGTTT
Details for BobuA.01025.a.A1.GE26473
HARVESTED ON: 4/20/2009
SEQUENCED ON: 5/6/2009
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEKENILEI KNLTIEFRLK HTTIHPVSNV NLSVKRGEIR AIVGESGSGK SVTSMAILKL LPELTTVYKS GEILFENQDL LKLSEKELLK IRGNKISMIF QDPMTSLNPF LRISTQLEET IILHQGLGKK EAKEKAIEML KTVGVVNAEE RIKHFPHQFS GGMRQRVMIA MALSCHPSLL IADEPTTALD VTIQEQILLL IKNLSKKFNT STIFITHDLA VVAEICDTVS VMYQGKIVEE GTVEEIFNNP KHPYTIGLLK SILTLEHDPN KKLYSTKENP MKITKTSTE
Validated NT Sequence
cctcggtgct ggtttttgtg atcttcatag ggttttcttt tgttgaataa agctttttat ttggatcgtg ttctagcgta agaattgatt ttaaaagccc aatggtgtaa ggatgcttag gattgttaaa tatttcctct actgttcctt cttctacaat ttttccttga tacattacag atactgtatc acaaatttca gcaacaaccg caagatcatg agttataaat atggtagaag tattgaattt tttagatagg tttttgatta ataataatat ttgctcttgg attgtaacat caagggctgt tgttggttca tctgctatta ataaggatgg atgacagcta agagccatgg caatcataac tctttgtctc attcctcctg aaaattgatg tgggaaatgt tttattcttt cttctgcgtt tacaacacca acagttttta acatttctat tgctttttct ttggcttctt ttttccctaa tccttggtgt aagattattg tttcttcaag ttgagttgat attcttaaaa atgggtttaa tgaagtcatt gggtcttgaa atatcattga tattttattc cctctgattt ttaaaagttc tttttcgcta agttttagca gatcttgatt ttcaaatagt atttctccac ttttatatac tgttgtaagt tctggtaata attttaaaat agccatactt gttacggatt ttccgcttcc agattctcca acaatagctc taatttctcc tctttttaca gataggttaa cattgcttac gggatgaatt gttgtatgtt ttaatctaaa ttcaattgtt aaatttttta tttccaatat attttctttt tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccatgg tntnncncct tnttaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEKENILEI KNLTIEFRLK HTTIHPVSNV NLSVKRGEIR AIVGESGSGK SVTSMAILKL LPELTTVYKS GEILFENQDL LKLSEKELLK IRGNKISMIF QDPMTSLNPF LRISTQLEET IILHQGLGKK EAKEKAIEML KTVGVVNAEE RIKHFPHQFS GGMRQRVMIA MALSCHPSLL IADEPTTALD VTIQEQILLL IKNLSKKFNT STIFITHDLA VVAEICDTVS VMYQGKIVEE GTVEEIFNNP KHPYTIGLLK SILTLEHDPN KKLYSTKENP MKITKTSTEE F
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaaaagaa aatatattgg aaataaaaaa tttaacaatt gaatttagat taaaacatac aacaattcat cccgtaagca atgttaacct atctgtaaaa agaggagaaa ttagagctat tgttggagaa tctggaagcg gaaaatccgt aacaagtatg gctattttaa aattattacc agaacttaca acagtatata aaagtggaga aatactattt gaaaatcaag atctgctaaa acttagcgaa aaagaacttt taaaaatcag agggaataaa atatcaatga tatttcaaga cccaatgact tcattaaacc catttttaag aatatcaact caacttgaag aaacaataat cttacaccaa ggattaggga aaaaagaagc caaagaaaaa gcaatagaaa tgttaaaaac tgttggtgtt gtaaacgcag aagaaagaat aaaacatttc ccacatcaat tttcaggagg aatgagacaa agagttatga ttgccatggc tcttagctgt catccatcct tattaatagc agatgaacca acaacagccc ttgatgttac aatccaagag caaatattat tattaatcaa aaacctatct aaaaaattca atacttctac catatttata actcatgatc ttgcggttgt tgctgaaatt tgtgatacag tatctgtaat gtatcaagga aaaattgtag aagaaggaac agtagaggaa atatttaaca atcctaagca tccttacacc attgggcttt taaaatcaat tcttacgcta gaacacgatc caaataaaaa gctttattca acaaaagaaa accctatgaa gatcacaaaa accagcaccg aggagtttta aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BobuA.01025.a.A1.PS00429
PURIFICATION DATe: 6/15/2009
CONCENTRATION: 29.41mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEKENILEI KNLTIEFRLK HTTIHPVSNV NLSVKRGEIR AIVGESGSGK SVTSMAILKL LPELTTVYKS GEILFENQDL LKLSEKELLK IRGNKISMIF QDPMTSLNPF LRISTQLEET IILHQGLGKK EAKEKAIEML KTVGVVNAEE RIKHFPHQFS GGMRQRVMIA MALSCHPSLL IADEPTTALD VTIQEQILLL IKNLSKKFNT STIFITHDLA VVAEICDTVS VMYQGKIVEE GTVEEIFNNP KHPYTIGLLK SILTLEHDPN KKLYSTKENP MKITKTSTE
Validated NT Sequence
cctcggtgct ggtttttgtg atcttcatag ggttttcttt tgttgaataa agctttttat ttggatcgtg ttctagcgta agaattgatt ttaaaagccc aatggtgtaa ggatgcttag gattgttaaa tatttcctct actgttcctt cttctacaat ttttccttga tacattacag atactgtatc acaaatttca gcaacaaccg caagatcatg agttataaat atggtagaag tattgaattt tttagatagg tttttgatta ataataatat ttgctcttgg attgtaacat caagggctgt tgttggttca tctgctatta ataaggatgg atgacagcta agagccatgg caatcataac tctttgtctc attcctcctg aaaattgatg tgggaaatgt tttattcttt cttctgcgtt tacaacacca acagttttta acatttctat tgctttttct ttggcttctt ttttccctaa tccttggtgt aagattattg tttcttcaag ttgagttgat attcttaaaa atgggtttaa tgaagtcatt gggtcttgaa atatcattga tattttattc cctctgattt ttaaaagttc tttttcgcta agttttagca gatcttgatt ttcaaatagt atttctccac ttttatatac tgttgtaagt tctggtaata attttaaaat agccatactt gttacggatt ttccgcttcc agattctcca acaatagctc taatttctcc tctttttaca gataggttaa cattgcttac gggatgaatt gttgtatgtt ttaatctaaa ttcaattgtt aaatttttta tttccaatat attttctttt tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccatgg tntnncncct tnttaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEKENILEI KNLTIEFRLK HTTIHPVSNV NLSVKRGEIR AIVGESGSGK SVTSMAILKL LPELTTVYKS GEILFENQDL LKLSEKELLK IRGNKISMIF QDPMTSLNPF LRISTQLEET IILHQGLGKK EAKEKAIEML KTVGVVNAEE RIKHFPHQFS GGMRQRVMIA MALSCHPSLL IADEPTTALD VTIQEQILLL IKNLSKKFNT STIFITHDLA VVAEICDTVS VMYQGKIVEE GTVEEIFNNP KHPYTIGLLK SILTLEHDPN KKLYSTKENP MKITKTSTEE F
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAAAAGAA AATATATTGG AAATAAAAAA TTTAACAATT GAATTTAGAT TAAAACATAC AACAATTCAT CCCGTAAGCA ATGTTAACCT ATCTGTAAAA AGAGGAGAAA TTAGAGCTAT TGTTGGAGAA TCTGGAAGCG GAAAATCCGT AACAAGTATG GCTATTTTAA AATTATTACC AGAACTTACA ACAGTATATA AAAGTGGAGA AATACTATTT GAAAATCAAG ATCTGCTAAA ACTTAGCGAA AAAGAACTTT TAAAAATCAG AGGGAATAAA ATATCAATGA TATTTCAAGA CCCAATGACT TCATTAAACC CATTTTTAAG AATATCAACT CAACTTGAAG AAACAATAAT CTTACACCAA GGATTAGGGA AAAAAGAAGC CAAAGAAAAA GCAATAGAAA TGTTAAAAAC TGTTGGTGTT GTAAACGCAG AAGAAAGAAT AAAACATTTC CCACATCAAT TTTCAGGAGG AATGAGACAA AGAGTTATGA TTGCCATGGC TCTTAGCTGT CATCCATCCT TATTAATAGC AGATGAACCA ACAACAGCCC TTGATGTTAC AATCCAAGAG CAAATATTAT TATTAATCAA AAACCTATCT AAAAAATTCA ATACTTCTAC CATATTTATA ACTCATGATC TTGCGGTTGT TGCTGAAATT TGTGATACAG TATCTGTAAT GTATCAAGGA AAAATTGTAG AAGAAGGAAC AGTAGAGGAA ATATTTAACA ATCCTAAGCA TCCTTACACC ATTGGGCTTT TAAAATCAAT TCTTACGCTA GAACACGATC CAAATAAAAA GCTTTATTCA ACAAAAGAAA ACCCTATGAA GATCACAAAA ACCAGCACCG AGGAGTTTTA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA