BobuA.01013.a
phosphoglycerate mutase (gpmA) (BB_0658)
| CENTER ID: | BobuA.01013.a |
| ORGANISM: | Borrelia burgdorferi B31 |
| ASSOCIATED DISEASE: | Lyme disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.01013.a.A1.GE26471 | Full length( BobuA.01013.a ) | 1 | 253 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4EMB
DEPOSITED: 4/11/2012
DETERMINATION: XRay
CLONE: BobuA.01013.a.A1.GE26471
PROTEIN: BobuA.01013.a.A1.PW26571
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|224326.49.peg.1049 |
| RefSeq: | NP_212792.1 |
| UniProt: | O51602 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLGDFMYKLV LVRHGESEWN KENLFTGWTD VKLSDKGIDE AVEAGLLLKQ EGYSFDIAFS SLLSRANDTL NIILRELGQS YISVKKTWRL NERHYGALQG LNKSETAAKY GEDKVLIWRR SYDVPPMSLD ESDDRHPIKD PRYKHIPKRE LPSTECLKDT VARVIPYWTD EIAKEVLEGK KVIVAAHGNS LRALVKYFDN LSEEDVLKLN IPTGIPLVYE LDKDLNPIKH YYLGDESKIK KAMESVASQG KLK
NT Sequence
ATGCTAGGAG ATTTTATGTA TAAATTAGTT TTAGTAAGAC ACGGAGAGAG TGAGTGGAAT AAAGAAAATC TTTTTACTGG TTGGACAGAT GTTAAACTTT CTGACAAGGG TATCGATGAG GCTGTTGAGG CGGGTTTGCT TCTCAAACAA GAAGGCTATT CTTTTGATAT TGCTTTTAGT TCTTTGTTGT CAAGAGCTAA TGACACTTTA AATATTATTT TGCGAGAATT AGGGCAATCT TATATTAGTG TAAAAAAAAC CTGGAGATTA AATGAAAGGC ACTATGGAGC TTTGCAAGGT TTAAATAAGT CAGAAACAGC TGCAAAATAT GGGGAAGATA AGGTTTTAAT TTGGAGACGT AGTTATGATG TGCCCCCAAT GTCTTTGGAT GAGTCTGATG ATCGTCATCC CATAAAAGAT CCAAGATATA AACATATCCC CAAAAGGGAA CTTCCTTCAA CAGAGTGCCT TAAAGATACT GTTGCAAGAG TTATTCCTTA TTGGACTGAT GAGATTGCAA AAGAAGTTCT TGAAGGTAAA AAAGTTATTG TTGCTGCTCA CGGTAATTCT TTAAGAGCGC TTGTTAAATA TTTTGACAAT TTAAGTGAAG AAGATGTTTT AAAGCTTAAC ATTCCCACAG GCATTCCTTT AGTTTACGAA TTAGATAAAG ATTTAAATCC CATTAAACAT TACTATCTAG GTGATGAGAG CAAAATTAAA AAGGCAATGG AATCTGTTGC TAGTCAAGGA AAGTTAAAG
Details for BobuA.01013.a.A1.GE26471
| HARVESTED ON: | 4/20/2009 |
| SEQUENCED ON: | 5/6/2009 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLGDFMYKL VLVRHGESEW NKENLFTGWT DVKLSDKGID EAVEAGLLLK QEGYSFDIAF SSLLSRANDT LNIILRELGQ SYISVKKTWR LNERHYGALQ GLNKSETAAK YGEDKVLIWR RSYDVPPMSL DESDDRHPIK DPRYKHIPKR ELPSTECLKD TVARVIPYWT DEIAKEVLEG KKVIVAAHGN SLRALVKYFD NLSEEDVLKL NIPTGIPLVY ELDKDLNPIK HYYLGDESKI KKAMESVASQ GKLK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctag gagattttat gtataaatta gttttagtaa gacacggaga gagtgagtgg aataaagaaa atctttttac tggttggaca gatgttaaac tttctgacaa gggtatcgat gaggctgttg aggcgggttt gcttctcaaa caagaaggct attcttttga tattgctttt agttctttgt tgtcaagagc taatgacact ttaaatatta ttttgcgaga attagggcaa tcttatatta gtgtaaaaaa aacctggaga ttaaatgaaa ggcactatgg agctttgcaa ggtttaaata agtcagaaac agctgcaaaa tatggggaag ataaggtttt aatttggaga cgtagttatg atgtgccccc aatgtctttg gatgagtctg atgatcgtca tcccataaaa gatccaagat ataaacatat ccccaaaagg gaacttcctt caacagagtg ccttaaagat actgttgcaa gagttatccc ttattggact gatgagattg caaaagaagt tcttgaaggt aaaaaagtta ttgttgctgc tcacggtaat tctttaagag cgcttgttaa atattttgac aatttaagtg aagaagatgt tttaaagctt aacattccca caggcattcc tttagtttac gaattagata aagatttaaa tcccattaaa cattactatc taggtgatga gagcaaaatt aaaaaggcaa tggaatctgt tgctagtcaa ggaaagttaa agtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLGDFMYKL VLVRHGESEW NKENLFTGWT DVKLSDKGID EAVEAGLLLK QEGYSFDIAF SSLLSRANDT LNIILRELGQ SYISVKKTWR LNERHYGALQ GLNKSETAAK YGEDKVLIWR RSYDVPPMSL DESDDRHPIK DPRYKHIPKR ELPSTECLKD TVARVIPYWT DEIAKEVLEG KKVIVAAHGN SLRALVKYFD NLSEEDVLKL NIPTGIPLVY ELDKDLNPIK HYYLGDESKI KKAMESVASQ GKLK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctaggagat tttatgtata aattagtttt agtaagacac ggagagagtg agtggaataa agaaaatctt tttactggtt ggacagatgt taaactttct gacaagggta tcgatgaggc tgttgaggcg ggtttgcttc tcaaacaaga aggctattct tttgatattg cttttagttc tttgttgtca agagctaatg acactttaaa tattattttg cgagaattag ggcaatctta tattagtgta aaaaaaacct ggagattaaa tgaaaggcac tatggagctt tgcaaggttt aaataagtca gaaacagctg caaaatatgg ggaagataag gttttaattt ggagacgtag ttatgatgtg cccccaatgt ctttggatga gtctgatgat cgtcatccca taaaagatcc aagatataaa catatcccca aaagggaact tccttcaaca gagtgcctta aagatactgt tgcaagagtt attccttatt ggactgatga gattgcaaaa gaagttcttg aaggtaaaaa agttattgtt gctgctcacg gtaattcttt aagagcgctt gttaaatatt ttgacaattt aagtgaagaa gatgttttaa agcttaacat tcccacaggc attcctttag tttacgaatt agataaagat ttaaatccca ttaaacatta ctatctaggt gatgagagca aaattaaaaa ggcaatggaa tctgttgcta gtcaaggaaa gttaaagtaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa