BobuA.01001.a

Cytidine monophosphate kinase (BB_0128)

CENTER ID:	BobuA.01001.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.01001.a.A1.GE26475	Full length( BobuA.01001.a )		1	221

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_133121
PATRIC ID:	fig\|224326.49.peg.526
RefSeq:	NP_212262.1
UniProt:	O51154

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIIAIDGPSA SGKSSIAREL GVRLNYKFIS SGHLYRIITL IAQRSLMNSC DFISEDSLLN LILENDISFN NFAFLLNGEN VENQILNDKI DFQVSFYSSY VGIRNIVNKK LREVVKFSDD NYIIEGRDIT TVVFPESEFK IYLDASVKVR ALRRYKQRNG NETLEELERT LKRRDDVDKK KQYGKLKLSK GVFYLDTSYK GLDDVCNIII EKFNLKKVRE R

NT Sequence

ATGATAATAG CAATTGATGG ACCTTCAGCA TCAGGAAAAA GTTCAATTGC AAGAGAGCTT GGCGTAAGGC TGAACTATAA ATTTATCAGC TCTGGGCATC TTTACAGGAT AATAACTTTA ATTGCTCAAA GATCTCTTAT GAATAGTTGC GATTTTATTA GTGAGGATAG TCTTTTAAAT TTGATTCTTG AGAATGATAT AAGTTTTAAT AATTTTGCCT TTTTGCTTAA TGGAGAGAAT GTTGAAAATC AAATTTTAAA CGATAAGATT GATTTTCAAG TTTCTTTTTA TTCTTCTTAT GTTGGAATAA GAAACATTGT GAATAAAAAA TTAAGAGAGG TTGTTAAATT TAGCGATGAT AATTATATAA TAGAAGGTAG AGATATTACT ACTGTAGTTT TTCCAGAATC TGAATTTAAA ATATATCTCG ATGCTTCTGT TAAAGTTCGA GCTTTGAGGC GATATAAGCA AAGAAATGGG AATGAGACCT TAGAAGAATT AGAGCGTACT CTTAAAAGAA GAGATGATGT TGACAAGAAA AAACAATATG GTAAGTTAAA ATTATCAAAA GGGGTTTTTT ACCTTGATAC AAGCTACAAA GGATTAGACG ATGTGTGTAA TATTATAATA GAAAAGTTTA ATTTGAAAAA AGTAAGAGAG AGG

Details for BobuA.01001.a.A1.GE26475

HARVESTED ON:	4/20/2009
SEQUENCED ON:	5/6/2009
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIIAIDGPS ASGKSSIARE LGVRLNYKFI SSGHLYRIIT LIAQRSLMNS CDFISEDSLL NLILENDISF NNFAFLLNGE NVENQILNDK IDFQVSFYSS YVGIRNIVNK KLREVVKFSD DNYIIEGRDI TTVVFPESEF KIYLDASVKV RALRRYKQRN GNETLEELER TLKRRDDVDK KKQYGKLKLS KGVFYLDTSY KGLDDVCNII IEKFNLKKVR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgataa tagcaattga tggaccttca gcatcaggaa aaagttcaat tgcaagagag cttggcgtaa ggctgaacta taaatttatc agctctgggc atctttacag gataataact ttaattgctc aaagatctct tatgaatagt tgcgatttta ttagtgagga tagtctttta aatttgattc ttgagaatga tataagtttt aataattttg cctttttgct taatggagag aatgttgaaa atcaaatttt aaacgataag attgattttc aagtttcttt ttattcttct tatgttggaa taagaaacat tgtgaataaa aaattaagag aggttgttaa atttagcgat gataattata taatagaagg tagagatatt actactgtag tttttccaga atctgaattt aaaatatatc tcgatgcttc tgttaaagtt cgagctttga ggcgatataa gcaaagaaat gggaatgaga ccttagaaga attagagcgt actcttaaaa gaagagatga tgttgacaag aaaaaacaat atggtaagtt aaaattatca aaaggggttt tttaccttga tacaagctac aaaggattag acgatgtgtg taatattata atagaaaagt ttaatttgaa aaaagtaaga g

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gataatagca attgatggac cttcagcatc aggaaaaagt tcaattgcaa gagagcttgg cgtaaggctg aactataaat ttatcagctc tgggcatctt tacaggataa taactttaat tgctcaaaga tctcttatga atagttgcga ttttattagt gaggatagtc ttttaaattt gattcttgag aatgatataa gttttaataa ttttgccttt ttgcttaatg gagagaatgt tgaaaatcaa attttaaacg ataagattga ttttcaagtt tctttttatt cttcttatgt tggaataaga aacattgtga ataaaaaatt aagagaggtt gttaaattta gcgatgataa ttatataata gaaggtagag atattactac tgtagttttt ccagaatctg aatttaaaat atatctcgat gcttctgtta aagttcgagc tttgaggcga tataagcaaa gaaatgggaa tgagacctta gaagaattag agcgtactct taaaagaaga gatgatgttg acaagaaaaa acaatatggt aagttaaaat tatcaaaagg ggttttttac cttgatacaa gctacaaagg attagacgat gtgtgtaata ttataataga aaagtttaat ttgaaaaaag taagagagag gtgaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa