SSGCID: Target Page

BobuA.00990.a

hypothetical protein

CENTER ID:	BobuA.00990.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00990.a.A1.GE26522	Full length( BobuA.00990.a )		1	201

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.646
RefSeq:	NP_212381.1
UniProt:	O51263

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTLFFATTN ENKINEVKNI LDIPNLNLIV PQNFNIKETG KTFKENSLLK AKALFEILNN NQNVFGEDSG LCIEALNLEP GIYSKRYDTY KLCKKLSTNE KNQLILDLMK NEKNRKAYFI CNISYISKNG QILNFEGIIK GKIALSLNDK KNYGFGYDSI FLTKNNKKLS DLTLEEKNKI SHRGIAFLKF KKFLLKSLFN S

NT Sequence

ATGAAAACAC TATTTTTTGC AACAACAAAT GAAAATAAAA TAAATGAAGT AAAAAATATA TTAGATATAC CTAATTTAAA CTTAATAGTC CCTCAAAATT TTAACATAAA AGAAACAGGA AAAACATTTA AAGAAAACTC TTTACTTAAA GCAAAAGCAC TGTTTGAAAT TTTAAATAAC AATCAAAATG TTTTCGGAGA AGATTCTGGG CTGTGCATTG AGGCACTAAA CTTGGAACCT GGAATTTACT CTAAAAGATA TGACACTTAT AAGCTATGTA AAAAATTAAG CACTAATGAA AAAAATCAAC TAATTTTGGA CTTAATGAAA AATGAAAAAA ACAGAAAAGC ATATTTTATA TGCAACATAA GCTATATATC AAAAAATGGG CAAATATTAA ATTTTGAAGG AATTATTAAG GGAAAAATTG CCTTAAGTTT AAATGACAAG AAAAACTATG GATTTGGGTA TGATTCAATA TTTTTAACTA AAAATAATAA AAAGCTTAGT GATCTAACAC TTGAAGAAAA AAACAAAATA TCCCACAGAG GAATAGCATT TTTAAAATTT AAAAAATTTT TACTAAAATC TTTATTCAAT AGT

Details for BobuA.00990.a.A1.GE26522

HARVESTED ON:	4/20/2009
SEQUENCED ON:	5/6/2009
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKTLFFATT NENKINEVKS ILDIPNLNLI VPQNFNIKET GKTFKENSLL KAKALFEILN NNQNVFGEDS GLCIEALNLE PGIYSKRYDT YKLCKKLSTN EKNQLILDLM KNEKNRKAYF ICNISYISKN GQILNFEGII KGKIALSLND KKNYGFGYDS IFLTKNNKKL SDLTLEEKNK ISHRGIAFLK FKKFLLKSLF

Validated NT Sequence

tgaataaaga ttttagtaaa aattttttaa attttaaaaa tgctattcct ctgtgggata ttttgttttt ttcttcaagt gttagatcac taagcttttt attattttta gttaaaaata ttgaatcata cccaaatcca tagtttttct tgtcatttaa acttaaggca atttttccct taataattcc ttcaaaattt aatatttgcc cattttttga tatatagctt atgttgcata taaaatatgc ttttctgttt ttttcatttt tcattaagtc caaaattagt tgattttttt cattagtgct taatttttta catagcttat aagtgtcata tcttttagag taaattccag gttccaagtt tagtgcctca atgcacagcc cagaatcttc tccgaaaaca ttttgattgt tatttaaaat ttcaaacagt gcttttgctt taagtaaaga gttttcttta aatgtttttc ctgtttcttt tatgttaaaa ttttgaggga ctattaagtt taaattaggt atatctaata tactttttac ttcatttatt ttattttcat ttgttgttgc aaaaaatagt gttttcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKTLFFATT NENKINEVKN ILDIPNLNLI VPQNFNIKET GKTFKENSLL KAKALFEILN NNQNVFGEDS GLCIEALNLE PGIYSKRYDT YKLCKKLSTN EKNQLILDLM KNEKNRKAYF ICNISYISKN GQILNFEGII KGKIALSLND KKNYGFGYDS IFLTKNNKKL SDLTLEEKNK ISHRGIAFLK FKKFLLKSLF NS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaacacta ttttttgcaa caacaaatga aaataaaata aatgaagtaa aaaatatatt agatatacct aatttaaact taatagtccc tcaaaatttt aacataaaag aaacaggaaa aacatttaaa gaaaactctt tacttaaagc aaaagcactg tttgaaattt taaataacaa tcaaaatgtt ttcggagaag attctgggct gtgcattgag gcactaaact tggaacctgg aatttactct aaaagatatg acacttataa gctatgtaaa aaattaagca ctaatgaaaa aaatcaacta attttggact taatgaaaaa tgaaaaaaac agaaaagcat attttatatg caacataagc tatatatcaa aaaatgggca aatattaaat tttgaaggaa ttattaaggg aaaaattgcc ttaagtttaa atgacaagaa aaactatgga tttgggtatg attcaatatt tttaactaaa aataataaaa agcttagtga tctaacactt gaagaaaaaa acaaaatatc ccacagagga atagcatttt taaaatttaa aaaattttta ctaaaatctt tattcaatag ttaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa