BobuA.00962.a

uracil-DNA glycosylase (BB_0053)

CENTER ID: BobuA.00962.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00962.a.A1.GE26419 Full length( BobuA.00962.a ) 1 223
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00962.a.A1.PS00259 Full length( BobuA.00962.a ) 1 223

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127417
PATRIC ID: fig|224326.49.peg.451
RefSeq: NP_212187.1
UniProt: O51082

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVKIEESWK EVLNNEFNKE YFKKLVKFIK HEYKTKNGKI FPPPKLIFNA FNSLPFKDIK VVIIGQDPYH GKNQANGLAF SVDSKIKIPP SLQNIFKEIE KSLKIKTIPN GDLKRWAIQG VFLINTILTV EEGKPSSHKA IGWEIFTDEV IKIISKNLKN IVFMLWGNLA RSKKGLIDPT KHLILETSHP SPYSANNGFL GSNHFSSALD YLKKHNKNII NFQ
NT Sequence
ATGAAAGTAA AAATTGAAGA ATCTTGGAAA GAAGTTTTAA ACAATGAATT TAACAAAGAG TACTTTAAAA AGCTCGTGAA ATTTATTAAA CATGAATATA AAACAAAAAA TGGAAAAATA TTTCCTCCCC CAAAACTCAT ATTCAATGCT TTTAATTCTT TACCATTTAA AGACATAAAA GTGGTAATAA TTGGACAAGA TCCATACCAT GGAAAAAACC AAGCAAATGG ACTTGCTTTT TCTGTAGATT CAAAAATTAA AATTCCACCA TCTTTGCAGA ACATATTTAA AGAAATAGAA AAAAGCTTAA AAATAAAAAC CATCCCAAAC GGAGATTTAA AAAGATGGGC AATTCAAGGT GTATTTCTAA TAAATACAAT TTTAACAGTC GAAGAAGGCA AACCTTCTTC TCACAAAGCT ATTGGGTGGG AAATTTTTAC AGATGAAGTA ATAAAAATAA TCTCTAAAAA TTTGAAAAAC ATTGTGTTTA TGCTTTGGGG CAATTTAGCA AGAAGTAAAA AAGGGCTAAT TGACCCAACA AAGCATCTAA TTCTTGAAAC AAGTCACCCA TCTCCATATT CAGCAAACAA TGGATTTTTA GGATCAAATC ATTTTAGCAG TGCTTTAGAT TATTTAAAAA AACACAATAA AAACATAATA AACTTTCAA
Details for BobuA.00962.a.A1.GE26419
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/1/2009
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKVKIEESW KEVLNNEFNK EYFKKLVKFI KHEYKTKNGK IFPPPKLIFN AFNSLPFKDI KVVIIGQDPY HGKNQANGLA FSVDSKIKIP PSLQNIFKEI EKSLKIKTIP NGDLKRWAIQ GVFLINTILT VEEGKPSSHK AIGWEIFTDE VIKIISKNLK NIVFMLWGNL ARSKKGLIDP TKHLILETSH PSPYSANNGF LGSNHFSSAL DYLKKHNKNI INFQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag taaaaattga agaatcttgg aaagaagttt taaacaatga atttaacaaa gagtacttta aaaagctcgt gaaatttatt aaacatgaat ataaaacaaa aaatggaaaa atatttcctc ccccaaaact catattcaat gcttttaatt ctttaccatt taaagacata aaagtggtaa taattggaca agatccatac catggaaaaa accaagcaaa tggacttgct ttttctgtag attcaaaaat taaaattcca ccatctttgc agaacatatt taaagaaata gaaaaaagct taaaaataaa aaccatccca aacggagatt taaaaagatg ggcaattcaa ggtgtatttc taataaatac aattttaaca gtcgaagaag gcaaaccttc ttctcacaaa gctattgggt gggaaatttt tacagatgaa gtaataaaaa taatctctaa aaatttgaaa aacattgtgt ttatgctttg gggcaattta gcaagaagta aaaaagggct aattgaccca acaaagcatc taattcttga aacaagtcac ccatctccat attcagcaaa caatggattt ttaggatcaa atcattttag cagtgcttta gattatttaa aaaaacacaa taaaaacata ataaactttc aataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVKIEESW KEVLNNEFNK EYFKKLVKFI KHEYKTKNGK IFPPPKLIFN AFNSLPFKDI KVVIIGQDPY HGKNQANGLA FSVDSKIKIP PSLQNIFKEI EKSLKIKTIP NGDLKRWAIQ GVFLINTILT VEEGKPSSHK AIGWEIFTDE VIKIISKNLK NIVFMLWGNL ARSKKGLIDP TKHLILETSH PSPYSANNGF LGSNHFSSAL DYLKKHNKNI INFQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagtaaaa attgaagaat cttggaaaga agttttaaac aatgaattta acaaagagta ctttaaaaag ctcgtgaaat ttattaaaca tgaatataaa acaaaaaatg gaaaaatatt tcctccccca aaactcatat tcaatgcttt taattcttta ccatttaaag acataaaagt ggtaataatt ggacaagatc cataccatgg aaaaaaccaa gcaaatggac ttgctttttc tgtagattca aaaattaaaa ttccaccatc tttgcagaac atatttaaag aaatagaaaa aagcttaaaa ataaaaacca tcccaaacgg agatttaaaa agatgggcaa ttcaaggtgt atttctaata aatacaattt taacagtcga agaaggcaaa ccttcttctc acaaagctat tgggtgggaa atttttacag atgaagtaat aaaaataatc tctaaaaatt tgaaaaacat tgtgtttatg ctttggggca atttagcaag aagtaaaaaa gggctaattg acccaacaaa gcatctaatt cttgaaacaa gtcacccatc tccatattca gcaaacaatg gatttttagg atcaaatcat tttagcagtg ctttagatta tttaaaaaaa cacaataaaa acataataaa ctttcaataa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BobuA.00962.a.A1.PS00259
PURIFICATION DATe: 7/6/2009
CONCENTRATION: 17.52mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKVKIEESW KEVLNNEFNK EYFKKLVKFI KHEYKTKNGK IFPPPKLIFN AFNSLPFKDI KVVIIGQDPY HGKNQANGLA FSVDSKIKIP PSLQNIFKEI EKSLKIKTIP NGDLKRWAIQ GVFLINTILT VEEGKPSSHK AIGWEIFTDE VIKIISKNLK NIVFMLWGNL ARSKKGLIDP TKHLILETSH PSPYSANNGF LGSNHFSSAL DYLKKHNKNI INFQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag taaaaattga agaatcttgg aaagaagttt taaacaatga atttaacaaa gagtacttta aaaagctcgt gaaatttatt aaacatgaat ataaaacaaa aaatggaaaa atatttcctc ccccaaaact catattcaat gcttttaatt ctttaccatt taaagacata aaagtggtaa taattggaca agatccatac catggaaaaa accaagcaaa tggacttgct ttttctgtag attcaaaaat taaaattcca ccatctttgc agaacatatt taaagaaata gaaaaaagct taaaaataaa aaccatccca aacggagatt taaaaagatg ggcaattcaa ggtgtatttc taataaatac aattttaaca gtcgaagaag gcaaaccttc ttctcacaaa gctattgggt gggaaatttt tacagatgaa gtaataaaaa taatctctaa aaatttgaaa aacattgtgt ttatgctttg gggcaattta gcaagaagta aaaaagggct aattgaccca acaaagcatc taattcttga aacaagtcac ccatctccat attcagcaaa caatggattt ttaggatcaa atcattttag cagtgcttta gattatttaa aaaaacacaa taaaaacata ataaactttc aataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVKIEESW KEVLNNEFNK EYFKKLVKFI KHEYKTKNGK IFPPPKLIFN AFNSLPFKDI KVVIIGQDPY HGKNQANGLA FSVDSKIKIP PSLQNIFKEI EKSLKIKTIP NGDLKRWAIQ GVFLINTILT VEEGKPSSHK AIGWEIFTDE VIKIISKNLK NIVFMLWGNL ARSKKGLIDP TKHLILETSH PSPYSANNGF LGSNHFSSAL DYLKKHNKNI INFQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAGTAAAA ATTGAAGAAT CTTGGAAAGA AGTTTTAAAC AATGAATTTA ACAAAGAGTA CTTTAAAAAG CTCGTGAAAT TTATTAAACA TGAATATAAA ACAAAAAATG GAAAAATATT TCCTCCCCCA AAACTCATAT TCAATGCTTT TAATTCTTTA CCATTTAAAG ACATAAAAGT GGTAATAATT GGACAAGATC CATACCATGG AAAAAACCAA GCAAATGGAC TTGCTTTTTC TGTAGATTCA AAAATTAAAA TTCCACCATC TTTGCAGAAC ATATTTAAAG AAATAGAAAA AAGCTTAAAA ATAAAAACCA TCCCAAACGG AGATTTAAAA AGATGGGCAA TTCAAGGTGT ATTTCTAATA AATACAATTT TAACAGTCGA AGAAGGCAAA CCTTCTTCTC ACAAAGCTAT TGGGTGGGAA ATTTTTACAG ATGAAGTAAT AAAAATAATC TCTAAAAATT TGAAAAACAT TGTGTTTATG CTTTGGGGCA ATTTAGCAAG AAGTAAAAAA GGGCTAATTG ACCCAACAAA GCATCTAATT CTTGAAACAA GTCACCCATC TCCATATTCA GCAAACAATG GATTTTTAGG ATCAAATCAT TTTAGCAGTG CTTTAGATTA TTTAAAAAAA CACAATAAAA ACATAATAAA CTTTCAATAA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA