BobuA.00952.a

UDP-N-acetylglucosamine 1-carboxyvinyltransferase (BB_0472)

CENTER ID:	BobuA.00952.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00952.a.A1.GE26424	Full length( BobuA.00952.a )		1	442

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131267
PATRIC ID:	fig\|224326.49.peg.864
RefSeq:	NP_212606.1
UniProt:	O51428

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFNFIKFLY IGGWIMHSYI VEGGYKIGGQ ITASGNKNAA LPCILAALLT DEEVILENIP NINDVKVVLD ILNDIGADIA REGNTLKIKV LNIVKTEIDS SFTDLIRASI LLLGPFVSRF GKIDMALPGG DVIGKRRLDT HFYGLCKLGA KLSTKDRRIV LKANKLVGAE MFLDEASVTA TENIIMAAVL AEGNTVIMNA ACEPHVQDLC NMLNSMGANI LGIGSNVLEI KGVKKLSGTV FRIGADFMQV GSLISLAALT GGELEIKKAD PQHFRLIRHV YSRLGINFEY DRENVYVRNK QELKVKLDFG GHIPKIDDGP WPAFPTDLMS IIVVTATQVE GTVLVFEKMF ESRMFFVDKL IKMGARIVLC DPHRVVVTGK SSLKGNVLSS PDVRAGMSLL IAAFVAEGRS EIQNVYQIER GYEDVVNKLI NLGAKIKKVK SQ

NT Sequence

GTGCGATTTA ATTTTATTAA GTTTTTATAT ATAGGAGGAT GGATTATGCA TAGTTATATT GTAGAAGGCG GCTATAAGAT AGGTGGTCAA ATTACAGCTA GTGGGAATAA GAACGCTGCT TTACCCTGTA TTTTGGCTGC TTTACTTACC GATGAAGAGG TTATTTTAGA AAATATTCCT AATATTAATG ATGTAAAAGT TGTTTTAGAT ATTTTAAATG ACATAGGAGC AGATATTGCA AGAGAGGGAA ATACTTTAAA AATAAAAGTT TTAAATATTG TGAAAACAGA AATAGATTCT TCTTTTACAG ATTTAATTAG GGCTTCCATA CTTTTATTAG GGCCTTTTGT TTCTAGGTTT GGAAAAATAG ATATGGCGCT TCCAGGAGGA GATGTGATTG GAAAGAGGAG GCTTGATACT CATTTTTACG GGCTTTGCAA GCTGGGGGCC AAGTTAAGCA CAAAAGATAG AAGGATTGTT TTAAAGGCTA ACAAGCTTGT TGGCGCTGAA ATGTTTTTAG ATGAAGCTTC TGTTACAGCC ACAGAAAATA TCATTATGGC TGCAGTTCTT GCTGAAGGAA ATACTGTTAT TATGAACGCT GCTTGTGAGC CACATGTTCA AGATTTGTGT AATATGTTAA ATTCAATGGG CGCTAATATT TTAGGAATTG GTTCAAATGT TTTAGAAATA AAAGGTGTAA AAAAATTAAG TGGGACCGTA TTTAGAATAG GAGCCGATTT CATGCAAGTT GGTTCTTTAA TTAGCCTTGC TGCATTAACA GGGGGTGAGT TGGAAATTAA AAAAGCAGAT CCCCAACATT TCAGATTAAT TAGGCATGTA TATTCAAGAC TTGGCATTAA TTTTGAATAT GACAGGGAAA ATGTATATGT AAGAAATAAA CAAGAATTAA AAGTTAAGTT AGATTTTGGT GGGCACATTC CAAAAATTGA TGATGGCCCA TGGCCAGCCT TTCCAACAGA CCTTATGAGT ATTATTGTAG TTACTGCAAC GCAAGTAGAA GGCACAGTTT TAGTTTTTGA GAAGATGTTT GAATCTAGGA TGTTTTTTGT AGATAAATTA ATAAAAATGG GTGCTCGAAT TGTGCTTTGT GATCCACACC GCGTAGTAGT TACGGGCAAA TCTTCTCTTA AAGGCAATGT TTTGTCTTCT CCGGATGTAC GAGCGGGAAT GTCTCTTCTT ATTGCTGCTT TTGTTGCTGA AGGTCGCAGC GAGATTCAAA ATGTTTATCA AATTGAAAGA GGATACGAAG ATGTAGTTAA CAAATTGATT AATTTGGGTG CAAAAATCAA GAAAGTTAAA AGTCAA

Details for BobuA.00952.a.A1.GE26424

HARVESTED ON:	4/9/2009
SEQUENCED ON:	5/8/2009
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVRFNFIKF LYIGGWIMHS YIVEGGYKIG GQITASGNKN AALPCILAAL LTDEEVILEN IPNINDVKVV LDILNDIGAD IAREGNTLKI KVLNIVKTEI DSSFTDLIRA SILLLGPFVS RFGKIDMALP GGDVIGKRRL DTHFYGLCKL GAKLSTKDRR IVLKANKLVG AEMFLDEASV TATENIIMAA VLAEGNTVIM NAACEPHVQD LCNMLNSMGA NILGIGSNVL EIKGVKKLSG TVFRIGADFM QVGSLISLAA LTGGELEIKK ADPQHFRLIR HVYSRLGINF EYDRENVYVR NKQELKVKLD FGGHIPKIDD GPWPAFPTDL MSIIVVTATQ VEGTVLVFEK MFESRMFFVD KLIKMGARIV LCDPHRVVVT GKSSLKGNVL SSPDVRAGMS LLIAAFVAEG RSEIQNVYQI ERGYEDVVNK LINLGAKIKK VKSQ

Validated NT Sequence

ttattattga cttttaactt tcttgatttt tgcacccaaa ttaatcaatt tgttaactac atcttcgtat cctctttcaa tttgataaac attttgaatc tcgctgcgac cttcagcaac aaaagcagca ataagaagag acattcccgc tcgtacatcc ggagaagaca aaacattgcc tttaagagaa gatttgcccg taactactac gcggtgtgga tcacaaagca caattcgagc acccattttt attaatttat ctacaaaaaa catcctagat tcaaacatct tctcaaaaac taaaactgtg ccttctactt gcgttgcagt aactacaata atactcataa ggtctgttgg aaaggctggc catgggccat catcaatttt tggaatgtgc ccaccaaaat ctaacttaac ttttaattct tgtttatttc ttacatatac attttccctg tcatattcaa aattaatgcc aagtcttgaa tatacatgcc taattaatct gaaatgttgg ggatctgctt ttttaatttc caactcaccc cctgttaatg cagcaaggct aattaaagaa ccaacttgca tgaaatcggc tcctattcta aatacggtcc cacttaattt ttttacacct tttatttcta aaacatttga accaattcct aaaatattag cgcccattga atttaacata ttacacaaat cttgaacatg tggctcacaa gcagcgttca taataacagt atttccttca gcaagaactg cagccataat gatattttct gtggctgtaa cagaagcttc atctaaaaac atttcagcgc caacaagctt gttagccttt aaaacaatcc ttctatcttt tgtgcttaac ttggccccca gcttgcaaag cccgtaaaaa tgagtatcaa gcctcctctt tccaatcaca tctcctcctg gaagcgccat atctattttt ccaaacctag aaacaaaagg ccctaataaa agtatggaag ccctaattaa atctgtaaaa gaagaatcta tttctgtttt cacaatattt aaaactttta tttttaaagt atttccctct cttgcaatat ctgctcctat gtcatttaaa atatctaaaa caacttttac atcattaata ttaggaatat tttctaaaat aacctcttca tcggtaagta aagcagccaa aatacagggt aaagcagcgt tcttattccc actagctgta atttgaccac ctatcttata gccgccttct acaatataac tatgcataat ccatcctcct atatataaaa acttaataaa attaaatcgc accatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgattt aattttatta agtttttata tataggagga tggattatgc atagttatat tgtagaaggc ggctataaga taggtggtca aattacagct agtgggaata agaacgctgc tttaccctgt attttggctg ctttacttac cgatgaagag gttattttag aaaatattcc taatattaat gatgtaaaag ttgttttaga tattttaaat gacataggag cagatattgc aagagaggga aatactttaa aaataaaagt tttaaatatt gtgaaaacag aaatagattc ttcttttaca gatttaatta gggcttccat acttttatta gggccttttg tttctaggtt tggaaaaata gatatggcgc ttccaggagg agatgtgatt ggaaagagga ggcttgatac tcatttttac gggctttgca agctgggggc caagttaagc acaaaagata gaaggattgt tttaaaggct aacaagcttg ttggcgctga aatgttttta gatgaagctt ctgttacagc cacagaaaat atcattatgg ctgcagttct tgctgaagga aatactgtta ttatgaacgc tgcttgtgag ccacatgttc aagatttgtg taatatgtta aattcaatgg gcgctaatat tttaggaatt ggttcaaatg ttttagaaat aaaaggtgta aaaaaattaa gtgggaccgt atttagaata ggagccgatt tcatgcaagt tggttcttta attagccttg ctgcattaac agggggtgag ttggaaatta aaaaagcaga tccccaacat ttcagattaa ttaggcatgt atattcaaga cttggcatta attttgaata tgacagggaa aatgtatatg taagaaataa acaagaatta aaagttaagt tagattttgg tgggcacatt ccaaaaattg atgatggccc atggccagcc tttccaacag accttatgag tattattgta gttactgcaa cgcaagtaga aggcacagtt ttagtttttg agaagatgtt tgaatctagg atgttttttg tagataaatt aataaaaatg ggtgctcgaa ttgtgctttg tgatccacac cgcgtagtag ttacgggcaa atcttctctt aaaggcaatg ttttgtcttc tccggatgta cgagcgggaa tgtctcttct tattgctgct tttgttgctg aaggtcgcag cgagattcaa aatgtttatc aaattgaaag aggatacgaa gatgtagtta acaaattgat taatttgggt gcaaaaatca agaaagttaa aagtcaatag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa