BobuA.00951.a

glu-tRNAamido transferase subunit A (gatA) (BB_0342)

CENTER ID: BobuA.00951.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00951.a.B1.GE39067 full length 1 481
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.738
RefSeq: NP_212476.2
UniProt: O51317

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLSNLTLTK IQELVLTRKC KIYDILLAYK NNYELNKDIN GYIEFFDDSL EIAKRYDDCL KNCELEDLPL IGMLIAVKDN ISIQDKSLTC ASEILKGYIS PYDATVIKRL KNKGAILIGR TNMDEFAMGS TCEFSYYGAT LNPLNREYVI GGSSGGSAAV VAAFQAPFSL GSDTGGSVRL PASFSGILGF KPSYGGLSRY GLASYASSFD QIGFFSHSIE DIALILKHTC GSDKMDSTSV DIFDDFYPLK IESLQGKNLA VIKELSEDLM DKNVANSFAK FKLDLLSKGI NIKEVSIEEI NFILSIYYII SPVEASSNLA RYTGLCYGKR ISEGLSLNDF YFKHRSNFLS EEVKRRIVLG NYLLSERYDS KYYAKACEIL QNLIIPKFNK LFESCDFIIT PTSFVKPFRL GLDFDDPVKM YYSDICTVIA NLIGAPAISL PYSKDEEGLS IGMQIIGRSK KDFELLSFSK NVIRELGLNG I
NT Sequence
ttggacttaa gtaatttaac tttaaccaaa attcaagaat tagttttaac tagaaaatgt aaaatttatg atattttgct tgcttataaa aataattatg agctaaataa agatatcaat ggatatattg aattttttga tgattcttta gagattgcaa aaaggtatga cgattgttta aaaaattgtg aattagaaga tttgccttta attggtatgc ttattgcagt caaagataat atttcaattc aagataaatc tttaacttgt gcttctgaga ttttaaaagg ttatatttct ccttatgatg cgactgttat taaaaggctt aagaataaag gagcaatttt aattggtaga accaatatgg atgaatttgc catgggttct acttgtgaat tttcttatta cggtgcaact ttaaatcctt taaatagaga atatgttata ggtggtagtt ctggaggctc tgcagctgta gttgcagctt ttcaagcacc tttttcgctt ggtagtgata ctggaggttc tgttaggctg cccgcatctt tttcaggaat tttgggtttt aaaccttctt atggaggtct ttctcgctat gggcttgcat cttatgcttc gtcttttgat caaataggat ttttttctca ttctattgaa gatattgctt taattttaaa gcatacttgt ggatctgata aaatggattc tactagtgta gacatttttg atgattttta tcctttaaaa attgagtcgt tgcaaggtaa aaatttagct gtaatcaaag agcttagcga agatctaatg gacaaaaatg ttgcaaatag ttttgcaaag tttaaattag accttttgtc aaagggtatt aatataaaag aagtttcaat agaagagatt aattttattt tatcaattta ttatataatt tctcctgttg aagcatcctc caatcttgct cgttatactg gactttgtta cggcaagaga atctctgaag gtttgagtct taatgatttt tattttaaac ataggagcaa tttcttgtca gaagaagtta aaaggcgtat tgttcttgga aattatttgt tatcagaaag gtatgattct aaatattatg caaaagcttg tgaaattctt caaaatttga ttattcctaa atttaacaag ctttttgaaa gctgtgattt tattattacc ccaacaagct ttgttaaacc ttttagactt ggtttggatt ttgatgatcc tgttaaaatg tattattcag atatttgtac tgttattgca aatcttattg gagcccctgc tatttcgctt ccatattcta aggatgagga aggattgtca attgggatgc aaattattgg gcgtagcaaa aaggattttg aacttttaag tttttcaaaa aatgtgatta gggaattagg attgaatgga atataa
Details for BobuA.00951.a.B1.GE39067
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML DLSNLTLTKI QELVLTRKCK IYDILLAYKN NYELNKDING YIEFFDDSLE IAKRYDDCLK NCELEDLPLI GMLIAVKDNI SIQDKSLTCA SENLKGYISP YDATVIKRLK NKGAILIGRT NMDEFAMGST CEFSYYGATL NPLKREYVIG GSSGGSAAVV AAFQAPFSLG SDTGGSVRLP ASFSGILGFK PSYGGLSRYG LASYASSFDQ IGFFSHSIED IALILKHTCG SDKMDSTSVD IFDDFYPLKI ESLQGKNLAV IKELSEDLMD KNVANSFAKF KLDLLSKGIN IKEVSIEEIN FILSIYYIIS PVEASSNLAR YTGLCYGKRI SEGLSLNDFY FKHRSNFLSE EVKRRIVLGN YLLSERYDSK YYAKACEILQ NLIIPKFNKL FESCDFIITP TSFVKPFRLG LDFDDPVKMY YSDICTVIAN LIGAPAISLP YSKDEEGLSI GMQIIGRSKK DFELLSFSKN V
Validated NT Sequence
aatcacattt tttgaaaaac ttaaaagttc aaaatccttt ttgctacgcc caataatttg catcccaatt gacaatcctt cctcatcctt agaatacgga agcgaaatag caggggctcc aataagattt gcaataacag tacaaatatc tgaataatac attttaacag gatcatcaaa atccaaacca agtctaaaag gtttaacaaa gcttgttggg gtaataataa aatcacagct ttcaaaaagc ttgttaaatt taggaataat caaattttga agaatttcac aagcttttgc ataatattta gaatcatacc tttctgataa caaataattt ccaagaacaa tacgcctttt aacttcttct gacaagaaat tgctcctatg tttaaaataa aaatcattaa gactcaaacc ttcagagatt ctcttgccgt aacaaagtcc agtataacga gcgagattgg aggatgcttc aacaggagaa attatataat aaattgataa aataaaatta atctcttcta tcgaaacttc ttttatatta ataccctttg acaaaaggtc taatttaaac tttgcaaaac tatttgcaac atttttgtcc attagatctt cgctaagctc tttgattaca gctaaatttt taccttgcaa cgactcaatt tttaaaggat aaaaatcatc aaaaatgtct acactagtag aatccatttt atcagatcca caagtatgct ttaaaattaa agcaatatct tcaatagaat gagaaaaaaa tcctatttga tcaaaagacg aagcataaga tgcaagccca tagcgagaaa gacctccata agaaggttta aaacccaaaa ttcctgaaaa agatgcgggt agcctaacag aacctccagt atcactacca agcgaaaaag gtgcttgaaa agctgcaact acagctgcag agcctccaga actaccacct ataacatatt ctctttttaa aggatttaaa gttgcaccgt aataagaaaa ttcacaagta gaacccatgg caaattcatc catattggtt ctaccaatta aaattgctcc tttattctta agccttttaa taacagtcgc atcataagga gaaatataac cttttaaatt ctcagaagca caagttaaag atttatcttg aattgaaata ttatctttga ctgcaataag cataccaatt aaaggcaaat cttctaattc acaatttttt aaacaatcgt catacctttt tgcaatctct aaagaatcat caaaaaattc aatatatcca ttgatatctt tatttagctc ataattattt ttataagcaa gcaaaatatc ataaatttta cattttctag ttaaaactaa ttcttgaatt ttggttaaag ttaaattact taagtccaac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMD LSNLTLTKIQ ELVLTRKCKI YDILLAYKNN YELNKDINGY IEFFDDSLEI AKRYDDCLKN CELEDLPLIG MLIAVKDNIS IQDKSLTCAS EILKGYISPY DATVIKRLKN KGAILIGRTN MDEFAMGSTC EFSYYGATLN PLNREYVIGG SSGGSAAVVA AFQAPFSLGS DTGGSVRLPA SFSGILGFKP SYGGLSRYGL ASYASSFDQI GFFSHSIEDI ALILKHTCGS DKMDSTSVDI FDDFYPLKIE SLQGKNLAVI KELSEDLMDK NVANSFAKFK LDLLSKGINI KEVSIEEINF ILSIYYIISP VEASSNLARY TGLCYGKRIS EGLSLNDFYF KHRSNFLSEE VKRRIVLGNY LLSERYDSKY YAKACEILQN LIIPKFNKLF ESCDFIITPT SFVKPFRLGL DFDDPVKMYY SDICTVIANL IGAPAISLPY SKDEEGLSIG MQIIGRSKKD FELLSFSKNV IRELGLNGI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggact taagtaattt aactttaacc aaaattcaag aattagtttt aactagaaaa tgtaaaattt atgatatttt gcttgcttat aaaaataatt atgagctaaa taaagatatc aatggatata ttgaattttt tgatgattct ttagagattg caaaaaggta tgacgattgt ttaaaaaatt gtgaattaga agatttgcct ttaattggta tgcttattgc agtcaaagat aatatttcaa ttcaagataa atctttaact tgtgcttctg agattttaaa aggttatatt tctccttatg atgcgactgt tattaaaagg cttaagaata aaggagcaat tttaattggt agaaccaata tggatgaatt tgccatgggt tctacttgtg aattttctta ttacggtgca actttaaatc ctttaaatag agaatatgtt ataggtggta gttctggagg ctctgcagct gtagttgcag cttttcaagc acctttttcg cttggtagtg atactggagg ttctgttagg ctgcccgcat ctttttcagg aattttgggt tttaaacctt cttatggagg tctttctcgc tatgggcttg catcttatgc ttcgtctttt gatcaaatag gatttttttc tcattctatt gaagatattg ctttaatttt aaagcatact tgtggatctg ataaaatgga ttctactagt gtagacattt ttgatgattt ttatccttta aaaattgagt cgttgcaagg taaaaattta gctgtaatca aagagcttag cgaagatcta atggacaaaa atgttgcaaa tagttttgca aagtttaaat tagacctttt gtcaaagggt attaatataa aagaagtttc aatagaagag attaatttta ttttatcaat ttattatata atttctcctg ttgaagcatc ctccaatctt gctcgttata ctggactttg ttacggcaag agaatctctg aaggtttgag tcttaatgat ttttatttta aacataggag caatttcttg tcagaagaag ttaaaaggcg tattgttctt ggaaattatt tgttatcaga aaggtatgat tctaaatatt atgcaaaagc ttgtgaaatt cttcaaaatt tgattattcc taaatttaac aagctttttg aaagctgtga ttttattatt accccaacaa gctttgttaa accttttaga cttggtttgg attttgatga tcctgttaaa atgtattatt cagatatttg tactgttatt gcaaatctta ttggagcccc tgctatttcg cttccatatt ctaaggatga ggaaggattg tcaattggga tgcaaattat tgggcgtagc aaaaaggatt ttgaactttt aagtttttca aaaaatgtga ttagggaatt aggattgaat ggaatatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t