SSGCID: Target Page

BobuA.00940.a

hypothetical protein

CENTER ID:	BobuA.00940.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00940.a.A1.GE26426	Full length( BobuA.00940.a )		1	230

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.518
RefSeq:	NP_212254.1
UniProt:	O51146

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKGSLPSHV GIIMDGNRRW ALGKRLSSLE GYKEGLKRAK EIVKYSLRVG IKCLSFYVFS TENWRRDDCE IENLMFLIAN YLRAEFDFYE KNGIKIIVSG DVESLSGEVK SSIKDSISFS KNFNNLILNL AINYGGRNEI LRAVKKFVSS DFDLESLNEN IFSSFLDNPE LPDLDLLIRT GGNIRISNFF LWRIAYSELI FSNVLWPEYY DTRYSKDLEC FQFRRRNFGR

NT Sequence

ATGAATAAAG GTTCTCTTCC AAGTCATGTT GGAATTATTA TGGATGGCAA TAGAAGGTGG GCTTTAGGTA AAAGATTGTC TTCTTTAGAA GGTTATAAAG AAGGTCTTAA AAGAGCAAAA GAAATAGTTA AGTATTCTTT AAGGGTAGGT ATTAAATGTT TATCCTTTTA TGTATTTTCT ACTGAGAATT GGAGAAGGGA TGATTGTGAG ATAGAAAATT TAATGTTTTT AATTGCTAAT TATTTGAGAG CCGAATTTGA TTTTTACGAA AAAAATGGAA TAAAAATAAT AGTTTCAGGA GATGTTGAGT CTTTAAGTGG GGAAGTAAAA AGTTCTATAA AAGACTCTAT TAGCTTTTCT AAAAATTTCA ACAACCTTAT TTTAAATTTA GCAATCAATT ATGGGGGTCG TAATGAAATA CTTAGAGCTG TTAAAAAATT TGTTTCAAGT GATTTTGATT TAGAGTCTTT GAATGAGAAT ATTTTTTCCA GTTTTTTAGA CAATCCAGAA CTACCCGATC TTGATCTTTT AATACGTACA GGCGGAAATA TTAGAATAAG TAATTTTTTC TTATGGAGGA TTGCTTATTC TGAATTAATT TTTTCAAATG TTTTGTGGCC TGAATATTAT GACACTAGAT ATAGTAAGGA TTTGGAATGT TTCCAATTTA GAAGAAGAAA TTTTGGGAGA

Details for BobuA.00940.a.A1.GE26426

HARVESTED ON:	4/9/2009
SEQUENCED ON:	5/1/2009
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNKGSLPSH VGIIMDGNRR WALGKRLSSL EGYKEGLKRA KEIVKYSLRV GIKCLSFYVF STENWRRDDC EIENLMFLIA NYLRAEFDFY EKNGIKIIVS GDVESLSGEV KSSIKDSISF SKNFNNLILN LAINYGGRNE ILRAVKKFVS SDFDLESLNE NIFSSFLDNP ELPDLDLLIR TGGNIRISNF FLWRIAYSEL IFSNVLWPEY YDTRYSKDLE CFQFRRRNFG R

Validated NT Sequence

ttattatctc ccaaaatttc ttcttctaaa ttggaaacat tccaaatcct tactatatct agtgtcataa tattcaggcc acaaaacatt tgaaaaaatt aattcagaat aagcaatcct ccataagaaa aaattactta ttctaatatt tccgcctgta cgtattaaaa gatcaagatc gggtagttct ggattgtcta aaaaactgga aaaaatattc tcattcaaag actctaaatc aaaatcactt gaaacaaatt ttttaacagc tctaagtatt tcattacgac ccccataatt gattgctaaa tttaaaataa ggttgttgaa atttttagaa aagctaatag agtcttttat agaacttttt acttccccac ttaaagactc aacatctcct gaaactatta tttttattcc atttttttcg taaaaatcaa attcggctct caaataatta gcaattaaaa acattaaatt ttctatctca caatcatccc ttctccaatt ctcagtagaa aatacataaa aggataaaca tttaatacct acccttaaag aatacttaac tatttctttt gctcttttaa gaccttcttt ataaccttct aaagaagaca atcttttacc taaagcccac cttctattgc catccataat aattccaaca tgacttggaa gagaaccttt attcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaataaaggt tctcttccaa gtcatgttgg aattattatg gatggcaata gaaggtgggc tttaggtaaa agattgtctt ctttagaagg ttataaagaa ggtcttaaaa gagcaaaaga aatagttaag tattctttaa gggtaggtat taaatgttta tccttttatg tattttctac tgagaattgg agaagggatg attgtgagat agaaaattta atgtttttaa ttgctaatta tttgagagcc gaatttgatt tttacgaaaa aaatggaata aaaataatag tttcaggaga tgttgagtct ttaagtgggg aagtaaaaag ttctataaaa gactctatta gcttttctaa aaatttcaac aaccttattt taaatttagc aatcaattat gggggtcgta atgaaatact tagagctgtt aaaaaatttg tttcaagtga ttttgattta gagtctttga atgagaatat tttttccagt tttttagaca atccagaact acccgatctt gatcttttaa tacgtacagg cggaaatatt agaataagta attttttctt atggaggatt gcttattctg aattaatttt ttcaaatgtt ttgtggcctg aatattatga cactagatat agtaaggatt tggaatgttt ccaatttaga agaagaaatt ttgggagatg aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa