BobuA.00915.a

plasmid partition protein, putative (BB_P32)

CENTER ID:	BobuA.00915.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00915.a.A1.GE26423	Full length( BobuA.00915.a )		1	246

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130366
PATRIC ID:	fig\|224326.49.peg.32
RefSeq:	NP_051193.1
UniProt:	Q45023

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTKKPKIIT IASIKGGVGK STSAIILATL LSKDNKVLLI DMDTQASVTS YFYKTLVESE FDLLEKNIYE VLKGNQLIND AIINVDHNFD LLPSYLSLHT FSEEPLPYKE HRLKDSFKYL KFKYNFIILD TNPHLDSTLS NALVVSKHVI VPMTAEKWTI ESLQLLEFFT DKLKLKPKVF LFVTKFKKNK THKDLLEMLQ KKEKFLGIIS EREDLNRRIA KNDRFDLDKD YIKEYVNVLN NFILKI

NT Sequence

ATGGATACTA AAAAACCTAA AATAATAACA ATAGCGTCAA TTAAGGGCGG TGTTGGCAAA AGCACAAGTG CAATAATTTT AGCAACGCTA TTATCAAAAG ACAACAAAGT ACTTTTAATT GATATGGATA CACAGGCTTC AGTTACTAGT TATTTTTATA AAACATTAGT AGAAAGTGAA TTTGATTTAC TTGAAAAAAA TATATATGAA GTTTTAAAAG GAAATCAATT AATAAATGAT GCAATTATCA ATGTTGATCA TAATTTTGAT TTGTTGCCAA GTTACTTAAG CTTGCACACT TTTAGTGAAG AGCCCTTGCC TTATAAGGAA CATAGGTTAA AGGATAGCTT TAAATATTTA AAATTTAAAT ATAATTTTAT TATACTTGAT ACTAATCCCC ATTTAGATTC TACGTTATCC AATGCTTTAG TTGTTAGTAA ACATGTTATA GTTCCAATGA CTGCAGAAAA GTGGACTATT GAGAGTTTGC AACTATTAGA GTTTTTTACG GATAAATTAA AGTTAAAACC CAAAGTATTT TTATTTGTAA CAAAATTTAA AAAAAATAAA ACTCATAAAG ATTTATTAGA AATGTTGCAA AAAAAAGAAA AGTTTTTGGG GATAATATCA GAACGTGAGG ATTTAAATAG GAGAATAGCA AAAAATGATA GATTTGATTT AGATAAAGAT TATATAAAGG AGTATGTAAA CGTTTTAAAT AATTTTATTT TGAAAATA

Details for BobuA.00915.a.A1.GE26423

HARVESTED ON:	4/9/2009
SEQUENCED ON:	5/1/2009
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDTKKPKII TIASIKGGVG KSTSAIILAT LLSKDNKVLL IDMDTQASVT SYFYKTLVES EFDLLEKNIY EVLKGNQLIN DAIINVDHNF DLLPSYLSLH TFSEEPLSYK EHRLKDSFKY LKFKYNFIIL DTNPHLDSTL SNALVVSKHV IVPMTAEKWT IESLQLLEFF TDKLKLKPKV FLFVTKFKKN KTHKDLLEML QKKEKFLGII SEREDLNRRI AKNDRFDLDK DYIKEYVNVL NNFIL

Validated NT Sequence

ttaagaagga gnnntaccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggatact aaaaaaccta aaataataac aatagcgtca attaagggcg gtgttggcaa aagcacaagt gcaataattt tagcaacgct attatcaaaa gacaacaaag tacttttaat tgatatggat acacaggctt cagttactag ttatttttat aaaacattag tagaaagtga atttgattta cttgaaaaaa atatatatga agttttaaaa ggaaatcaat taataaatga tgcaattatc aatgttgatc ataattttga tttgttgcca agttacttaa gcttgcacac ttttagtgaa gagcccttgt cttataagga acataggtta aaggatagct ttaaatattt aaaatttaaa tataatttta ttatacttga tactaatccc catttagatt ctacgttatc caatgcttta gttgttagta aacatgttat agttccaatg actgcagaaa agtggactat tgagagtttg caactattag agttttttac ggataaatta aagttaaaac ccaaagtatt tttatttgta acaaaattta aaaaaaataa aactcataaa gatttattag aaatgttgca aaaaaaagaa aagtttttgg ggataatatc agaacgtgag gatttaaata ggagaatagc aaaaaatgat agatttgatt tagataaaga ttatataaag gagtatgtaa acgttttaaa taattttatt ttga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTKKPKII TIASIKGGVG KSTSAIILAT LLSKDNKVLL IDMDTQASVT SYFYKTLVES EFDLLEKNIY EVLKGNQLIN DAIINVDHNF DLLPSYLSLH TFSEEPLPYK EHRLKDSFKY LKFKYNFIIL DTNPHLDSTL SNALVVSKHV IVPMTAEKWT IESLQLLEFF TDKLKLKPKV FLFVTKFKKN KTHKDLLEML QKKEKFLGII SEREDLNRRI AKNDRFDLDK DYIKEYVNVL NNFILKI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatactaaa aaacctaaaa taataacaat agcgtcaatt aagggcggtg ttggcaaaag cacaagtgca ataattttag caacgctatt atcaaaagac aacaaagtac ttttaattga tatggataca caggcttcag ttactagtta tttttataaa acattagtag aaagtgaatt tgatttactt gaaaaaaata tatatgaagt tttaaaagga aatcaattaa taaatgatgc aattatcaat gttgatcata attttgattt gttgccaagt tacttaagct tgcacacttt tagtgaagag cccttgcctt ataaggaaca taggttaaag gatagcttta aatatttaaa atttaaatat aattttatta tacttgatac taatccccat ttagattcta cgttatccaa tgctttagtt gttagtaaac atgttatagt tccaatgact gcagaaaagt ggactattga gagtttgcaa ctattagagt tttttacgga taaattaaag ttaaaaccca aagtattttt atttgtaaca aaatttaaaa aaaataaaac tcataaagat ttattagaaa tgttgcaaaa aaaagaaaag tttttgggga taatatcaga acgtgaggat ttaaatagga gaatagcaaa aaatgataga tttgatttag ataaagatta tataaaggag tatgtaaacg ttttaaataa ttttattttg aaaatatgaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a