BobuA.00907.a

ATP-dependent protease ATP-binding subunit (BB_0295)

CENTER ID:	BobuA.00907.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00907.a.A1.GE26422	Full length( BobuA.00907.a )		1	455

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129971
PATRIC ID:	fig\|224326.49.peg.694
RefSeq:	NP_212429.1
UniProt:	Q44772

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCLRRLKMNK LEEHYIVPKD VVAELDKYII GQDEAKKLVS IALVNRYIRS RLPKEIKDEV MPKNIIMIGS TGIGKTEIAR RLSKLIKAPF IKVEATKYTE VGYVGRDVES MVRDLMSIAV NMVKEEMYST VRDDALVRTE ERIVDSLFKG SSNSENMDPN EIKAEEKVKE KLRKKLRAGE LDDTTIEIQI SSKMPFSTIE IFTGGNFEEI DMGIGGLLGN IFDRKKKREL KIKKAKEIIL AEELEKLVDH ENISDIAKSK VENMGIIFID EIDKIAAKNR SGNDVSREGV QRDILPIIEG SKVNTKYGIV DTSHILFIAA GAFNLAKPSD LIPELQGRFP IKVELKSLSI DDLKKILKQT KNSLIKQYVA MFKVYDLDLK FSEEAIDRIA ELTFNMNLES ENLGARRLHG VMEIVLADLF FEVPGSKLKK FEINLDYVNK KIQINEQKDL NYYII

NT Sequence

TTGTGCTTGA GGAGATTGAA AATGAATAAA TTAGAAGAGC ACTATATAGT TCCCAAAGAT GTAGTTGCAG AACTTGATAA ATATATAATA GGTCAAGACG AAGCTAAAAA ATTAGTATCA ATTGCTCTTG TTAATAGATA TATAAGGTCT AGGCTTCCAA AAGAAATAAA AGATGAGGTA ATGCCTAAAA ACATTATTAT GATTGGATCA ACTGGCATTG GGAAGACCGA GATTGCAAGA AGACTTTCTA AATTAATTAA AGCTCCTTTT ATTAAAGTTG AGGCTACAAA ATATACTGAG GTTGGTTATG TTGGTCGTGA TGTTGAATCT ATGGTTAGAG ATTTAATGAG CATTGCAGTT AATATGGTAA AAGAAGAGAT GTATAGTACT GTAAGAGATG ATGCTTTAGT AAGAACAGAG GAGAGAATAG TTGATAGTCT TTTTAAGGGA TCTAGTAATT CTGAGAATAT GGATCCAAAT GAAATAAAGG CGGAAGAAAA GGTAAAAGAG AAGCTTAGAA AAAAGCTTAG AGCAGGTGAG CTTGATGATA CTACTATTGA AATACAAATT TCTAGTAAAA TGCCATTTTC TACAATAGAA ATATTTACGG GTGGTAATTT TGAAGAGATT GATATGGGAA TTGGCGGTTT GCTGGGTAAT ATATTTGATA GAAAAAAGAA AAGAGAATTG AAGATTAAAA AAGCAAAGGA AATAATATTA GCAGAAGAGC TTGAGAAATT GGTCGATCAC GAAAACATTT CAGATATTGC AAAATCTAAA GTTGAAAATA TGGGAATTAT TTTTATTGAT GAGATCGATA AAATAGCTGC TAAGAATAGG AGTGGCAATG ATGTGTCCAG AGAAGGTGTT CAAAGAGATA TTTTGCCAAT TATTGAAGGT TCTAAAGTTA ATACAAAATA TGGAATAGTT GATACTTCTC ATATTTTATT TATTGCAGCA GGAGCATTTA ATTTAGCTAA ACCTTCTGAT TTAATACCCG AGCTTCAAGG AAGATTTCCT ATTAAGGTTG AACTTAAGAG CTTAAGCATA GACGATTTGA AAAAAATTTT AAAACAAACA AAAAATTCTT TAATAAAACA ATATGTTGCG ATGTTTAAGG TTTATGATTT AGATTTAAAG TTTAGTGAGG AAGCTATAGA TAGAATTGCA GAGCTTACTT TTAATATGAA TCTTGAGAGT GAAAATCTTG GTGCCAGAAG ACTTCACGGT GTTATGGAAA TAGTGCTTGC AGATCTTTTT TTTGAAGTGC CTGGCAGTAA GTTGAAAAAA TTTGAAATAA ACTTGGACTA TGTTAATAAA AAAATACAAA TTAACGAACA AAAAGATTTG AATTATTATA TAATT

Details for BobuA.00907.a.A1.GE26422

HARVESTED ON:	4/9/2009
SEQUENCED ON:	5/1/2009
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MGTLEAQTQG PGSMLCLRRL KMNKLEEHYI VPKDVVAELD KYIIGQDEAK KLVSIALVNR YIRSRLPKEI KDEVMPKNII MIGSTGIGKT EIARRLSKLI KAPFIKVEAT KYTEVGYVGR DVESMVRDLM SIAVNMVKEE MYSTVRDDAL VRTEERIVDS LFKGSSNSEN MDPNEIKAEE KVKEKLRKKL RAGELDDTTI EIQISSKMPF STIEXFTGGN FEEIDMGIGG LLGNIFDRKK KRELKIKKAK EIILAEELEK LVDHENISDI AKSKVENMGI IFIDEIDKIA AKNRSGNDVS REGVQRDILP IIEGSKVNTK YGIVDTSHIL FIAAGAFNLA KPSDLIPELQ GRFPIKVELK SLSIDDLKKI LKQTKNSLIK QYVAMFKVYD LDLKFSEEAI DRIAELTFNM NLESENLGAR RLHGVMEIVL ADLFFEVPGS KLKKFEINLD YVNKKIQINE QKDLNYYII

Validated NT Sequence

ttattaaatt atataataat tcaaatcttt ttgttcgtta atttgtattt ttttattaac atagtccaag tttatttcaa attttttcaa cttactgcca ggcacttcaa aaaaaagatc tgcaagcact atttccataa caccgtgaag tcttctggca ccaagatttt cactctcaag attcatatta aaagtaagct ctgcaattct atctatagct tcctcactaa actttaaatc taaatcataa accttaaaca tcgcaacata ttgttttatt aaagaatttt ttgtttgttt taaaattttt ttcaaatcgt ctatgcttaa gctcttaagt tcaaccttaa taggaaatct tccttgaagc tcgggtatta aatcagaagg tttagctaaa ttaaatgctc ctgctgcaat aaataaaata tgagaagtat caactattcc atattttgta ttaactttag aaccttcaat aattggcaaa atatctcttt gaacaccttc tctggacaca tcattgccac tcctattctt agcagctatt ttatcgatct catcaataaa aataattccc atattttcaa ctttagattt tgcaatatct gaaatgtttt cgtgatcgac caatttctca agctcttctg ctaatattat ttcctttgct tttttaatct tcaattctct tttctttttt ctatcaaata tattacccag caaaccgcca attcccatat caatctcttc aaaattacca cccgtaaata nttctattgt agaaaatggc attttactag aaatttgtat ttcaatagta gtatcatcaa gctcacctgc tctaagcttt tttctaagct tctcttttac cttttcttcc gcctttattt catttggatc catattctca gaattactag atcccttaaa aagactatca actattctct cctctgttct tactaaagca tcatctctta cagtactata catctcttct tttaccatat taactgcaat gctcattaaa tctctaacca tagattcaac atcacgacca acataaccaa cctcagtata ttttgtagcc tcaactttaa taaaaggagc tttaattaat ttagaaagtc ttcttgcaat ctcggtcttc ccaatgccag ttgatccaat cataataatg tttttaggca ttacctcatc ttttatttct tttggaagcc tagaccttat atatctatta acaagagcaa ttgatactaa ttttttagct tcgtcttgac ctattatata tttatcaagt tctgcaacta catctttggg aactatatag tgctcttcta atttattcat tttcaatctc ctcaagcaca acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLCLRRLKM NKLEEHYIVP KDVVAELDKY IIGQDEAKKL VSIALVNRYI RSRLPKEIKD EVMPKNIIMI GSTGIGKTEI ARRLSKLIKA PFIKVEATKY TEVGYVGRDV ESMVRDLMSI AVNMVKEEMY STVRDDALVR TEERIVDSLF KGSSNSENMD PNEIKAEEKV KEKLRKKLRA GELDDTTIEI QISSKMPFST IEIFTGGNFE EIDMGIGGLL GNIFDRKKKR ELKIKKAKEI ILAEELEKLV DHENISDIAK SKVENMGIIF IDEIDKIAAK NRSGNDVSRE GVQRDILPII EGSKVNTKYG IVDTSHILFI AAGAFNLAKP SDLIPELQGR FPIKVELKSL SIDDLKKILK QTKNSLIKQY VAMFKVYDLD LKFSEEAIDR IAELTFNMNL ESENLGARRL HGVMEIVLAD LFFEVPGSKL KKFEINLDYV NKKIQINEQK DLNYYII

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtgcttg aggagattga aaatgaataa attagaagag cactatatag ttcccaaaga tgtagttgca gaacttgata aatatataat aggtcaagac gaagctaaaa aattagtatc aattgctctt gttaatagat atataaggtc taggcttcca aaagaaataa aagatgaggt aatgcctaaa aacattatta tgattggatc aactggcatt gggaagaccg agattgcaag aagactttct aaattaatta aagctccttt tattaaagtt gaggctacaa aatatactga ggttggttat gttggtcgtg atgttgaatc tatggttaga gatttaatga gcattgcagt taatatggta aaagaagaga tgtatagtac tgtaagagat gatgctttag taagaacaga ggagagaata gttgatagtc tttttaaggg atctagtaat tctgagaata tggatccaaa tgaaataaag gcggaagaaa aggtaaaaga gaagcttaga aaaaagctta gagcaggtga gcttgatgat actactattg aaatacaaat ttctagtaaa atgccatttt ctacaataga aatatttacg ggtggtaatt ttgaagagat tgatatggga attggcggtt tgctgggtaa tatatttgat agaaaaaaga aaagagaatt gaagattaaa aaagcaaagg aaataatatt agcagaagag cttgagaaat tggtcgatca cgaaaacatt tcagatattg caaaatctaa agttgaaaat atgggaatta tttttattga tgagatcgat aaaatagctg ctaagaatag gagtggcaat gatgtgtcca gagaaggtgt tcaaagagat attttgccaa ttattgaagg ttctaaagtt aatacaaaat atggaatagt tgatacttct catattttat ttattgcagc aggagcattt aatttagcta aaccttctga tttaataccc gagcttcaag gaagatttcc tattaaggtt gaacttaaga gcttaagcat agacgatttg aaaaaaattt taaaacaaac aaaaaattct ttaataaaac aatatgttgc gatgtttaag gtttatgatt tagatttaaa gtttagtgag gaagctatag atagaattgc agagcttact tttaatatga atcttgagag tgaaaatctt ggtgccagaa gacttcacgg tgttatggaa atagtgcttg cagatctttt ttttgaagtg cctggcagta agttgaaaaa atttgaaata aacttggact atgttaataa aaaaatacaa attaacgaac aaaaagattt gaattattat ataatttaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a