BobuA.00876.a

spermidine/putrescine ABC transporter, ATP-binding protein (potA) (BB_0642)

CENTER ID:	BobuA.00876.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00876.a.A1.PW26440	Full length( BobuA.00876.a )		1	347

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127485
PATRIC ID:	fig\|224326.49.peg.1033
RefSeq:	NP_212776.1
UniProt:	O51587

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDNCILEIKN LSHYYDNNGN KTLDNINLKI KKNEFITLLG PSGCGKTTLI KILGGFLSQK NGEIYFFSKE ISKTSPNKRE INTVFQNYAL FPHMNVFDNI SFGLRMKKTP KDIIKEKVKT SLSLIGMPKY AYRNINELSG GQKQRVAIAR AMVMEPKLLL LDEPLSALDL KMRQEMQKEL KKIQRQLGIT FIYVTHDQEE ALTMSDRIVV MNEGIILQIG TPEEIYNEPK TKFVADFIGE SNIFDGTYKK ELVVSLLGHE FECLDKGFEA EEAVDLVIRP EDVKLLPKGK GHLSGTITSA IFQGVHYEMT LEIQKTNWIV QSTRLTKVGE EVDIFLEPDD IHVMHKE

NT Sequence

TTGGATAATT GTATCCTAGA GATTAAAAAT CTAAGTCATT ATTATGATAA CAATGGAAAC AAAACTTTAG ATAACATAAA TTTAAAAATT AAAAAAAATG AGTTTATCAC ACTACTAGGC CCATCCGGAT GTGGAAAAAC AACATTGATA AAAATATTGG GTGGTTTTTT AAGCCAAAAA AATGGAGAAA TTTATTTCTT TTCTAAAGAA ATATCTAAAA CCAGTCCAAA CAAAAGAGAA ATTAATACTG TATTTCAAAA TTATGCACTT TTCCCACATA TGAATGTTTT TGACAATATT TCATTTGGAC TTAGAATGAA AAAAACGCCA AAAGATATAA TCAAAGAAAA AGTAAAAACA TCGCTTTCGC TGATAGGAAT GCCAAAATAC GCATACAGAA ATATTAACGA ACTATCGGGG GGGCAAAAGC AAAGAGTTGC AATAGCAAGA GCAATGGTAA TGGAACCTAA GCTTTTACTC CTAGATGAAC CACTTTCCGC GCTTGATTTG AAAATGCGAC AAGAGATGCA AAAAGAATTA AAAAAAATAC AGCGTCAGCT TGGAATCACA TTCATATATG TTACTCACGA TCAAGAAGAG GCATTGACAA TGAGTGACAG AATCGTTGTA ATGAATGAAG GAATAATTCT GCAAATAGGA ACACCTGAGG AAATTTACAA TGAGCCTAAA ACAAAGTTTG TAGCCGATTT TATTGGAGAA AGCAATATTT TTGATGGAAC ATATAAAAAA GAGCTGGTTG TAAGTTTGCT TGGTCATGAA TTTGAATGCC TTGACAAAGG ATTTGAAGCT GAAGAAGCAG TTGACCTTGT AATACGCCCA GAAGATGTAA AACTACTTCC AAAAGGAAAA GGACATTTAA GCGGAACTAT AACATCAGCA ATTTTTCAAG GAGTTCATTA CGAAATGACT CTAGAAATCC AAAAAACAAA TTGGATAGTT CAAAGCACAA GACTTACAAA AGTTGGAGAA GAAGTTGATA TATTTTTAGA ACCTGATGAT ATTCATGTTA TGCATAAGGA A

Details for BobuA.00876.a.A1.PW26440

PURIFICATION DATe:	3/8/2010
CONCENTRATION:	40.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	100µl
PERCENT IDENTITY:	82
PERCENT COVERAGE:	107

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLDNCILEI KNLSHYYDNN GNKTLDNINL KIKKNEFITL LGPSGCGKTT LIKILGGFLS QKNGEIYFFS KEISKTSPNK REINTVFQNY ALFPHMNVFD NISFGLRMKK TPKDIIKEKV KTSLSLIGMP KYAYRNINEL SGGQKQRVAI ARAMVMEPKL LLLDEPLSAL DLKMRQEMQK ELKKIQRQLG ITFIYVTHDQ EEALTMSDRI VVMNEGIILQ IGTPEEIYNE PKTKFVADFI GESNIFDGTY KKELXVSLLG HEFECLDKGF EAEXAVDLVI XPRRCKTTSK XKRTFKRNYX SAXFXEXXTK XPXNXKNXXX XXXXXLXSXX XXXXFXXXXX XXXXXXXXXX XAXXXXXXXX XXXXXXXXXX XXXXX

Validated NT Sequence

ttttnnttna ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg ataattgtat cctagagatt aaaaatctaa gtcattatta tgataacaat ggaaacaaaa ctttagataa cataaattta aaaattaaaa aaaatgagtt tatcacacta ctaggcccat ccggatgtgg aaaaacaaca ttgataaaaa tattgggtgg ttttttaagc caaaaaaatg gagaaattta tttcttttct aaagaaatat ctaaaaccag tccaaacaaa agagaaatta atactgtatt tcaaaattat gcacttttcc cacatatgaa tgtttttgac aatatttcat ttggacttag aatgaaaaaa acgccaaaag atataatcaa agaaaaagta aaaacatcgc tttcgctgat aggaatgcca aaatacgcat acagaaatat taacgaacta tcgggggggc aaaagcaaag agttgcaata gcaagagcaa tggtaatgga acctaagctt ttactcctag atgaaccact ttccgcgctt gatttgaaaa tgcgacaaga gatgcaaaaa gaattaaaaa aaatacagcg tcagcttgga atcacattca tatatgttac tcacgatcaa gaagaggcat tgacaatgag tgacagaatc gttgtaatga atgaaggaat aattctgcaa ataggaacac ctgaggaaat ttacaatgag cctaaaacaa agtttgtagc cgattttatt ggagaaagca atatttttga tggaacatat aaaaaagagc tggntgtaag tttgcttggt catgaatttg aatgccttga caaaggattt gaagctgaan aagcagttga ccttgtaata cnncccagaa gatgtaaaac tacttccaaa nggaaaagga cntttaagcg gaactatana tcagcanttt tcanggagnt catnacnaaa tgncctngna atcnaaaaaa cnannnnnnn nnnnnaagnn nnanactnna aagtgnanaa nantnnnnnn tttttnnanc tgannnnnnn nngntnngcn nnnnnnnnnn ncnnannnnt nnngcnnnnn ncntnnnnnn nnnnnnnnnn tgnnnnnnnn nnnnnnnnnn nnnnnnannn ncnnnnnnnn nnnannna

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLDNCILEI KNLSHYYDNN GNKTLDNINL KIKKNEFITL LGPSGCGKTT LIKILGGFLS QKNGEIYFFS KEISKTSPNK REINTVFQNY ALFPHMNVFD NISFGLRMKK TPKDIIKEKV KTSLSLIGMP KYAYRNINEL SGGQKQRVAI ARAMVMEPKL LLLDEPLSAL DLKMRQEMQK ELKKIQRQLG ITFIYVTHDQ EEALTMSDRI VVMNEGIILQ IGTPEEIYNE PKTKFVADFI GESNIFDGTY KKELVVSLLG HEFECLDKGF EAEEAVDLVI RPEDVKLLPK GKGHLSGTIT SAIFQGVHYE MTLEIQKTNW IVQSTRLTKV GEEVDIFLEP DDIHVMHKE

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGATAAT TGTATCCTAG AGATTAAAAA TCTAAGTCAT TATTATGATA ACAATGGAAA CAAAACTTTA GATAACATAA ATTTAAAAAT TAAAAAAAAT GAGTTTATCA CACTACTAGG CCCATCCGGA TGTGGAAAAA CAACATTGAT AAAAATATTG GGTGGTTTTT TAAGCCAAAA AAATGGAGAA ATTTATTTCT TTTCTAAAGA AATATCTAAA ACCAGTCCAA ACAAAAGAGA AATTAATACT GTATTTCAAA ATTATGCACT TTTCCCACAT ATGAATGTTT TTGACAATAT TTCATTTGGA CTTAGAATGA AAAAAACGCC AAAAGATATA ATCAAAGAAA AAGTAAAAAC ATCGCTTTCG CTGATAGGAA TGCCAAAATA CGCATACAGA AATATTAACG AACTATCGGG GGGGCAAAAG CAAAGAGTTG CAATAGCAAG AGCAATGGTA ATGGAACCTA AGCTTTTACT CCTAGATGAA CCACTTTCCG CGCTTGATTT GAAAATGCGA CAAGAGATGC AAAAAGAATT AAAAAAAATA CAGCGTCAGC TTGGAATCAC ATTCATATAT GTTACTCACG ATCAAGAAGA GGCATTGACA ATGAGTGACA GAATCGTTGT AATGAATGAA GGAATAATTC TGCAAATAGG AACACCTGAG GAAATTTACA ATGAGCCTAA AACAAAGTTT GTAGCCGATT TTATTGGAGA AAGCAATATT TTTGATGGAA CATATAAAAA AGAGCTGGTT GTAAGTTTGC TTGGTCATGA ATTTGAATGC CTTGACAAAG GATTTGAAGC TGAAGAAGCA GTTGACCTTG TAATACGCCC AGAAGATGTA AAACTACTTC CAAAAGGAAA AGGACATTTA AGCGGAACTA TAACATCAGC AATTTTTCAA GGAGTTCATT ACGAAATGAC TCTAGAAATC CAAAAAACAA ATTGGATAGT TCAAAGCACA AGACTTACAA AAGTTGGAGA AGAAGTTGAT ATATTTTTAG AACCTGATGA TATTCATGTT ATGCATAAGG AATAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA