SSGCID: Target Page

BobuA.00863.a

sensory transduction histidine kinase, putative (BB_0764)

CENTER ID:	BobuA.00863.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00863.a.A1.GE26416	Full length( BobuA.00863.a )		1	382

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00863.a.A1.PS00311	Full length( BobuA.00863.a )		1	382

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127115
PATRIC ID:	fig\|224326.49.peg.1155
RefSeq:	NP_212898.1
UniProt:	O51705

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNFFKKALT KLNKLSNEQK TKFIEQIYKK IEIYDGIFAS INEGIIVLDK QNNIIYANKI LYQILALTSK SKIEILDDIQ IPNLINLIKE LVRTEDKIIG LEVPISNGIY IKISFMPYVK EKKLEGNIIL IEDIKEKKKK EELFRRVEAL ASFTRHARNI AHEIKNPLGA IDINLQLLKK EIEKQKMKNG KAENYFKVIK EEINRVDKIV TEFLLTVRPI KINLQEKDIK QVIGSVCELL NPGLENKHIK LLLNLNKISN ILIDEKLLKQ VIINIVKNAE EALLETKKEI KKIEIFLFEK DNKIHINIKD NGNGIKDGVK EEIFKPQFST KEKGSGIGLT ISYKIIKELG GEIFVESKEG KGTIFTITLP KLNKKNILIE GY

NT Sequence

ATGAATAATT TTTTCAAAAA AGCTTTAACA AAGCTAAACA AATTATCTAA CGAACAAAAA ACTAAATTTA TTGAACAAAT TTACAAAAAA ATAGAAATAT ATGACGGAAT ATTTGCATCA ATTAATGAAG GAATCATTGT ACTTGACAAA CAAAACAATA TAATCTATGC AAACAAGATT TTATACCAAA TTTTAGCTTT AACATCTAAA TCAAAAATAG AAATTCTTGA TGACATTCAA ATTCCAAACT TAATAAATTT AATAAAAGAA CTAGTTAGAA CAGAAGATAA AATAATAGGA TTAGAAGTTC CAATCTCAAA CGGCATATAT ATTAAAATCT CATTTATGCC TTATGTAAAA GAAAAAAAAC TTGAAGGCAA CATTATTTTA ATCGAAGACA TTAAAGAGAA AAAAAAGAAA GAGGAACTAT TTAGAAGAGT TGAGGCTTTG GCCTCTTTTA CAAGGCATGC AAGAAATATT GCCCATGAAA TCAAAAACCC ACTTGGAGCA ATCGATATAA ATTTACAACT GCTAAAAAAG GAAATTGAAA AACAAAAAAT GAAAAATGGT AAAGCTGAAA ATTATTTTAA AGTAATAAAA GAAGAAATAA ACAGAGTAGA TAAAATAGTA ACAGAATTTT TACTAACTGT CAGACCAATA AAAATTAACT TACAAGAAAA AGATATTAAA CAAGTAATAG GCAGCGTATG TGAATTGTTA AATCCTGGAT TAGAAAATAA ACACATAAAA CTATTGCTTA ATTTAAACAA AATAAGCAAT ATTCTCATTG ATGAAAAACT ATTAAAACAA GTTATTATAA ACATCGTTAA AAACGCAGAA GAAGCACTGC TTGAAACAAA AAAAGAAATA AAAAAAATAG AAATTTTTCT CTTCGAAAAA GACAATAAAA TACATATCAA CATAAAAGAT AACGGAAACG GAATAAAAGA TGGGGTAAAA GAGGAAATAT TTAAGCCTCA ATTTAGCACA AAAGAAAAAG GAAGTGGAAT AGGACTTACT ATTTCTTATA AAATAATAAA AGAGCTTGGA GGTGAAATTT TTGTGGAAAG CAAAGAGGGC AAAGGCACTA TTTTTACAAT TACGCTGCCT AAACTAAATA AAAAAAATAT TTTAATTGAA GGGTAT

Details for BobuA.00863.a.A1.GE26416

HARVESTED ON:	4/9/2009
SEQUENCED ON:	5/8/2009
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNNFFKKAL TKLNKLSNEQ KTKFIEQIYK KIEIYDGIFA SINEGIIVLD KQNNIIYANK ILYQILALTS KSKIEILDDI QIPNLINLIK ELVRTEDKII GLEVPISNGI YIKISFMPYV KEKKLEGNII LIEDIKEKKK KEELFRRVEA LASFTRHARN IAHEIKNPLG AIDINLQLLK KEIEKQKMKN GKAENYFKVI KEEINRVDKI VTEFLLTVRP IKINLQEKDI KQVIGSVCEL LNPGLENKHI KLLLNLNKIS NILIDEKLLK QVIINIVKNA EEALLETKKE IKKIEIFLFE KDNKIHINIK DNGNGIKDGV KEEIFKPQFS TKEKGSGIGL TISYKIIKEL GGEIFVESKE GKGTIFTITL PKLNKKNILI EGY

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata attttttcaa aaaagcttta acaaagctaa acaaattatc taacgaacaa aaaactaaat ttattgaaca aatttacaaa aaaatagaaa tatatgacgg aatatttgca tcaattaatg aaggaatcat tgtacttgac aaacaaaaca atataatcta tgcaaacaag attttatacc aaattttagc tttaacatct aaatcaaaaa tagaaattct tgatgacatt caaattccaa acttaataaa tttaataaaa gaactagtta gaacagaaga taaaataata ggattagaag ttccaatctc aaacggcata tatattaaaa tctcatttat gccttatgta aaagaaaaaa aacttgaagg caacattatt ttaatcgaag acattaaaga gaaaaaaaag aaagaggaac tatttagaag agttgaggct ttggcctctt ttacaaggca tgcaagaaat attgcccatg aaatcaaaaa cccacttgga gcaatcgata taaatttaca actgctaaaa aaggaaattg aaaaacaaaa aatgaaaaat ggtaaagctg aaaattattt taaagtaata aaagaagaaa taaacagagt agataaaata gtaacagaat ttttactaac tgtcagacca ataaaaatta acttacaaga aaaagatatt aaacaagtaa taggcagcgt atgtgaattg ttaaatcctg gattagaaaa taaacacata aaactattgc ttaatttaaa caaaataagc aatattctca ttgatgaaaa actattaaaa caagttatta taaacatcgt taaaaacgca gaagaagcac tgcttgaaac aaaaaaagaa ataaaaaaaa tagaaatttt tctcttcgaa aaagacaata aaatacatat caacataaaa gataacggaa acggaataaa agatggggta aaagaggaaa tatttaagcc tcaatttagc acaaaagaaa aaggaagtgg aataggactt actatttctt ataaaataat aaaagagctt ggaggtgaaa tttttgtgga aagcaaagag ggcaaaggca ctatttttac aattacgctg cctaaactaa ataaaaaaaa tattttaatt gaagggtatt aataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaataatttt ttcaaaaaag ctttaacaaa gctaaacaaa ttatctaacg aacaaaaaac taaatttatt gaacaaattt acaaaaaaat agaaatatat gacggaatat ttgcatcaat taatgaagga atcattgtac ttgacaaaca aaacaatata atctatgcaa acaagatttt ataccaaatt ttagctttaa catctaaatc aaaaatagaa attcttgatg acattcaaat tccaaactta ataaatttaa taaaagaact agttagaaca gaagataaaa taataggatt agaagttcca atctcaaacg gcatatatat taaaatctca tttatgcctt atgtaaaaga aaaaaaactt gaaggcaaca ttattttaat cgaagacatt aaagagaaaa aaaagaaaga ggaactattt agaagagttg aggctttggc ctcttttaca aggcatgcaa gaaatattgc ccatgaaatc aaaaacccac ttggagcaat cgatataaat ttacaactgc taaaaaagga aattgaaaaa caaaaaatga aaaatggtaa agctgaaaat tattttaaag taataaaaga agaaataaac agagtagata aaatagtaac agaattttta ctaactgtca gaccaataaa aattaactta caagaaaaag atattaaaca agtaataggc agcgtatgtg aattgttaaa tcctggatta gaaaataaac acataaaact attgcttaat ttaaacaaaa taagcaatat tctcattgat gaaaaactat taaaacaagt tattataaac atcgttaaaa acgcagaaga agcactgctt gaaacaaaaa aagaaataaa aaaaatagaa atttttctct tcgaaaaaga caataaaata catatcaaca taaaagataa cggaaacgga ataaaagatg gggtaaaaga ggaaatattt aagcctcaat ttagcacaaa agaaaaagga agtggaatag gacttactat ttcttataaa ataataaaag agcttggagg tgaaattttt gtggaaagca aagagggcaa aggcactatt tttacaatta cgctgcctaa actaaataaa aaaaatattt taattgaagg gtattgaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BobuA.00863.a.A1.PS00311

PURIFICATION DATe:	8/12/2009
CONCENTRATION:	24.3mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	50µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATAATTTT TTCAAAAAAG CTTTAACAAA GCTAAACAAA TTATCTAACG AACAAAAAAC TAAATTTATT GAACAAATTT ACAAAAAAAT AGAAATATAT GACGGAATAT TTGCATCAAT TAATGAAGGA ATCATTGTAC TTGACAAACA AAACAATATA ATCTATGCAA ACAAGATTTT ATACCAAATT TTAGCTTTAA CATCTAAATC AAAAATAGAA ATTCTTGATG ACATTCAAAT TCCAAACTTA ATAAATTTAA TAAAAGAACT AGTTAGAACA GAAGATAAAA TAATAGGATT AGAAGTTCCA ATCTCAAACG GCATATATAT TAAAATCTCA TTTATGCCTT ATGTAAAAGA AAAAAAACTT GAAGGCAACA TTATTTTAAT CGAAGACATT AAAGAGAAAA AAAAGAAAGA GGAACTATTT AGAAGAGTTG AGGCTTTGGC CTCTTTTACA AGGCATGCAA GAAATATTGC CCATGAAATC AAAAACCCAC TTGGAGCAAT CGATATAAAT TTACAACTGC TAAAAAAGGA AATTGAAAAA CAAAAAATGA AAAATGGTAA AGCTGAAAAT TATTTTAAAG TAATAAAAGA AGAAATAAAC AGAGTAGATA AAATAGTAAC AGAATTTTTA CTAACTGTCA GACCAATAAA AATTAACTTA CAAGAAAAAG ATATTAAACA AGTAATAGGC AGCGTATGTG AATTGTTAAA TCCTGGATTA GAAAATAAAC ACATAAAACT ATTGCTTAAT TTAAACAAAA TAAGCAATAT TCTCATTGAT GAAAAACTAT TAAAACAAGT TATTATAAAC ATCGTTAAAA ACGCAGAAGA AGCACTGCTT GAAACAAAAA AAGAAATAAA AAAAATAGAA ATTTTTCTCT TCGAAAAAGA CAATAAAATA CATATCAACA TAAAAGATAA CGGAAACGGA ATAAAAGATG GGGTAAAAGA GGAAATATTT AAGCCTCAAT TTAGCACAAA AGAAAAAGGA AGTGGAATAG GACTTACTAT TTCTTATAAA ATAATAAAAG AGCTTGGAGG TGAAATTTTT GTGGAAAGCA AAGAGGGCAA AGGCACTATT TTTACAATTA CGCTGCCTAA ACTAAATAAA AAAAATATTT TAATTGAAGG GTATTGAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA