BobuA.00845.a

phosphate ABC transporter, ATP-binding protein (pstB) (BB_0218)

CENTER ID: BobuA.00845.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00845.a.A1.GE26417 Full length( BobuA.00845.a ) 1 260
BobuA.00845.a.B1.GE27143 Full length( BobuA.00845.a ) 1 260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_130152
PATRIC ID: fig|224326.49.peg.616
RefSeq: NP_212352.1
UniProt: O51236
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKEKDTPKN EVIIETENLN LFYTDFKALN KINMKILKNS ITALIGPSGC GKSTFLRTLN RMNDLVEGIK IEGNVIYEGK NIYSNNFDIL ELRRKIGMVF QTPNPFLMSI YDNISYGPKI HGTKDKKKLD EIVEQSLKKS ALWNEVKDKL HTNALSLSGG QQQRLCIART LAIEPNVILM DEPTSALDPI STGKIEELII NLKESYTIII VTHNMQQAGR ISDRTAFFLN GCIEEESSTD ELFFNPKNTK TEEYISGKFG
NT Sequence
GTGATAAAAG AAAAAGATAC ACCAAAAAAT GAAGTTATAA TAGAAACGGA AAATCTGAAT CTATTTTATA CAGACTTTAA AGCTTTAAAT AAAATAAACA TGAAAATACT GAAAAATAGT ATTACCGCCT TAATAGGCCC ATCTGGCTGT GGTAAATCAA CATTCTTAAG AACTCTCAAC AGAATGAACG ATCTTGTTGA AGGCATTAAA ATAGAAGGCA ATGTAATATA TGAGGGAAAA AACATTTATT CAAATAATTT CGATATCCTG GAGCTTAGAA GAAAAATAGG AATGGTATTC CAAACCCCTA ACCCATTTTT AATGTCTATC TATGATAATA TAAGCTATGG TCCAAAAATA CATGGAACAA AAGATAAAAA AAAACTAGAC GAAATAGTAG AGCAATCACT AAAAAAATCT GCACTCTGGA ATGAGGTCAA AGACAAACTT CATACAAACG CCTTAAGTCT CTCAGGAGGC CAGCAGCAAA GGCTCTGTAT TGCAAGAACT CTCGCAATAG AGCCAAATGT AATACTGATG GACGAACCTA CCTCTGCTCT CGATCCAATA TCAACAGGCA AAATTGAAGA GTTAATAATA AACTTAAAAG AAAGTTACAC AATAATCATT GTAACTCACA ACATGCAACA AGCCGGAAGA ATATCTGATA GAACCGCATT TTTCCTTAAT GGATGCATTG AAGAAGAAAG TTCAACTGAT GAATTATTCT TCAATCCTAA AAATACTAAA ACTGAAGAAT ATATCAGCGG AAAATTTGGA
Details for BobuA.00845.a.A1.GE26417
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/1/2009
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MVIKEKDTPK NEVIIETENL NLFYTDFKAL NKINMKILKN SITALIGPSG CGKSTFLRTL NRMNDLVEGI KIEGNVIYEG KNIYSNNFDI LELRRKIGMV FQTPNPFLMS IYDNISYGPK IHGTKDKKKL DEIVEQSLKK SALWNEVKDK LHTNALSLSG GQQQRLCIAR TLAIEPNVIL MDEPTSALDP ISTGKIEELI INLKESYTII IVTHNMQQAG RISDRTAFFL NGCIEEESST DELFFNPKNT KTEEYISGKF G
Validated NT Sequence
ttattatcca aattttccgc tgatatattc ttcagtttta gtatttttag gattgaagaa taattcatca gttgaacttt cttcttcaat gcatccatta aggaaaaatg cggttctatc agatattctt ccggcttgtt gcatgttgtg agttacaatg attattgtgt aactttcttt taagtttatt attaactctt caattttgcc tgttgatatt ggatcgagag cagaggtagg ttcgtccatc agtattacat ttggctctat tgcgagagtt cttgcaatac agagcctttg ctgctggcct cctgagagac ttaaggcgtt tgtatgaagt ttgtctttga cctcattcca gagtgcagat ttttttagtg attgctctac tatttcgtct agtttttttt tatcttttgt tccatgtatt tttggaccat agcttatatt atcatagata gacattaaaa angggttagg ggtttggaan accattccta tttttcttct aagctccagg atatcgaaat tatttgaata aatgtttttt ccctcatata ttacattgcc ttctatttta atgccttcaa caagatcgtt cattctgttg agagttctta agaatgttga tttaccacag ccagatgggc ctattaaggc ggtaatacta tttttcagta ttttcatgtt tattttattt aaagctttaa agtctgtata aaatagattc agattttccg tttctattat aacttcattt tttggtgtat ctttttcttt tatcaccatc gaaccaggac cctgggtctg agcttccagg gtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIKEKDTP KNEVIIETEN LNLFYTDFKA LNKINMKILK NSITALIGPS GCGKSTFLRT LNRMNDLVEG IKIEGNVIYE GKNIYSNNFD ILELRRKIGM VFQTPNPFLM SIYDNISYGP KIHGTKDKKK LDEIVEQSLK KSALWNEVKD KLHTNALSLS GGQQQRLCIA RTLAIEPNVI LMDEPTSALD PISTGKIEEL IINLKESYTI IIVTHNMQQA GRISDRTAFF LNGCIEEESS TDELFFNPKN TKTEEYISGK FG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgataaaa gaaaaagata caccaaaaaa tgaagttata atagaaacgg aaaatctgaa tctattttat acagacttta aagctttaaa taaaataaac atgaaaatac tgaaaaatag tattaccgcc ttaataggcc catctggctg tggtaaatca acattcttaa gaactctcaa cagaatgaac gatcttgttg aaggcattaa aatagaaggc aatgtaatat atgagggaaa aaacatttat tcaaataatt tcgatatcct ggagcttaga agaaaaatag gaatggtatt ccaaacccct aacccatttt taatgtctat ctatgataat ataagctatg gtccaaaaat acatggaaca aaagataaaa aaaaactaga cgaaatagta gagcaatcac taaaaaaatc tgcactctgg aatgaggtca aagacaaact tcatacaaac gccttaagtc tctcaggagg ccagcagcaa aggctctgta ttgcaagaac tctcgcaata gagccaaatg taatactgat ggacgaacct acctctgctc tcgatccaat atcaacaggc aaaattgaag agttaataat aaacttaaaa gaaagttaca caataatcat tgtaactcac aacatgcaac aagccggaag aatatctgat agaaccgcat ttttccttaa tggatgcatt gaagaagaaa gttcaactga tgaattattc ttcaatccta aaaatactaa aactgaagaa tatatcagcg gaaaatttgg ataaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BobuA.00845.a.B1.GE27143
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV IKEKDTPKNE VIIETENLNL FYTDFKALNK INMKILKNSI TALIGPSGCG KSTFLRTLNR MNDLVEGIKI EGNVIYEGKN IYSNNFDILE LRRKIGMVFQ TPNPFLMSIY DNISYGPKIH GTKDKKKLDE IVEQSLKKSA LWNEVKDKLH TNALSLSGGQ QQRLCIARTL AIEPNVILMD EPTSALDPIS TGKIEELIIN LKESYTIIIV THNMQQAGRI SDRTAFFLNG CIEEESSTDE LFFNPKNTKT EEYISGKFG
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tggtgataaa agaaaaagat acaccaaaaa atgaagttat aatagaaacg gaaaatctga atctatttta tacagacttt aaagctttaa ataaaataaa catgaaaata ctgaaaaata gtattaccgc cttaataggc ccatctggct gtggtaaatc aacattctta agaactctca acagaatgaa cgatcttgtt gaaggcatta aaatagaagg caatgtaata tatgagggaa aaaacattta ttcaaataat ttcgatatcc tggagcttag aagaaaaata ggaatggtat tccaaacccc taacccattt ttaatgtcta tctatgataa tataagctat ggtccaaaaa tacatggaac aaaagataaa aaaaaactag acgaaatagt agagcaatca ctaaaaaaat ctgcactctg gaatgaggtc aaagacaaac ttcatacaaa cgccttaagt ctctcaggag gccagcagca aaggctctgt attgcaagaa ctctcgcaat agagccaaat gtaatactga tggacgaacc tacctctgct ctcgatccaa tatcaacagg caaaattgaa gagttaataa taaacttaaa agaaagttac acaataatca ttgtaactca caacatgcaa caagccggaa gaatatctga tagaaccgca tttttcctta atggatgcat tgaagaagaa agttcaactg atgaattatt cttcaatcct aaaaatacta aaactgaaga atatatcagc ggaaaatttg gataagtgag taagat
Expected Protein Sequence
MAHHHHHHMV IKEKDTPKNE VIIETENLNL FYTDFKALNK INMKILKNSI TALIGPSGCG KSTFLRTLNR MNDLVEGIKI EGNVIYEGKN IYSNNFDILE LRRKIGMVFQ TPNPFLMSIY DNISYGPKIH GTKDKKKLDE IVEQSLKKSA LWNEVKDKLH TNALSLSGGQ QQRLCIARTL AIEPNVILMD EPTSALDPIS TGKIEELIIN LKESYTIIIV THNMQQAGRI SDRTAFFLNG CIEEESSTDE LFFNPKNTKT EEYISGKFG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga taaaagaaaa agatacacca aaaaatgaag ttataataga aacggaaaat ctgaatctat tttatacaga ctttaaagct ttaaataaaa taaacatgaa aatactgaaa aatagtatta ccgccttaat aggcccatct ggctgtggta aatcaacatt cttaagaact ctcaacagaa tgaacgatct tgttgaaggc attaaaatag aaggcaatgt aatatatgag ggaaaaaaca tttattcaaa taatttcgat atcctggagc ttagaagaaa aataggaatg gtattccaaa cccctaaccc atttttaatg tctatctatg ataatataag ctatggtcca aaaatacatg gaacaaaaga taaaaaaaaa ctagacgaaa tagtagagca atcactaaaa aaatctgcac tctggaatga ggtcaaagac aaacttcata caaacgcctt aagtctctca ggaggccagc agcaaaggct ctgtattgca agaactctcg caatagagcc aaatgtaata ctgatggacg aacctacctc tgctctcgat ccaatatcaa caggcaaaat tgaagagtta ataataaact taaaagaaag ttacacaata atcattgtaa ctcacaacat gcaacaagcc ggaagaatat ctgatagaac cgcatttttc cttaatggat gcattgaaga agaaagttca actgatgaat tattcttcaa tcctaaaaat actaaaactg aagaatatat cagcggaaaa tttggataat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat