BobuA.00743.a

tryptophanyl-tRNA synthetase (BB_0005)

CENTER ID: BobuA.00743.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00743.a.A1.GE26404 Full length( BobuA.00743.a ) 1 353
BobuA.00743.a.B1.GE27142 Full length( BobuA.00743.a ) 1 353
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127531
PATRIC ID: fig|224326.49.peg.403
RefSeq: NP_212139.1
UniProt: O51038
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILKRKVMLT GDRPTGALHL GHYVGSVVNR LKFQEEYETY FIIADLHTLT TKPDLKSINT IPSNVREMVL DYLACGINPD KVSIYLQSAI PELFELHLIF SMIVTVARLQ RIPSIKDMSI AAGLKEIPYG LLGYPVLMSA DILMTKANLV PVGRDNESHI EFARELARRF NHLYKNNFFP IPESVFTDSR PLVGIYGKNK MSKSLDNAIF LNDDENLLEK KIMSMYTDPN RIRADIPGNV EGNPIFIYHS FFNSNHEEVE DLKSRYKKGK VGDVEVKKKL FLALNSFLKP IRDKRSFYEA KKKDYIDEII FDGTSKARFI ANKVVKDVKD LIGLSKTWNG IKYSAEKKLK NEE
NT Sequence
TTGATTTTGA AAAGAAAGGT TATGCTTACG GGAGATAGGC CTACTGGCGC TCTTCATTTG GGGCACTATG TGGGGTCTGT AGTAAATCGG TTAAAATTTC AAGAAGAGTA TGAAACATAT TTTATTATAG CCGATTTACA TACTTTGACT ACAAAGCCTG ACTTGAAGAG CATCAATACA ATACCTTCTA ATGTTAGAGA AATGGTTTTA GATTATCTTG CTTGTGGAAT TAATCCTGAT AAGGTTAGTA TATATCTGCA ATCAGCCATA CCCGAGCTTT TTGAGCTTCA TTTAATATTT TCAATGATTG TTACTGTTGC GCGCTTGCAA AGGATTCCAA GTATAAAAGA CATGAGCATT GCAGCTGGAC TTAAAGAGAT TCCTTATGGC CTTTTGGGGT ATCCTGTTCT TATGAGTGCA GATATTTTAA TGACAAAAGC AAATTTAGTT CCCGTTGGGC GTGATAATGA ATCTCATATT GAATTTGCAA GAGAGCTTGC ACGAAGGTTT AACCATTTGT ACAAAAATAA TTTTTTCCCA ATACCCGAAT CTGTTTTCAC AGATTCTCGT CCTTTGGTGG GTATTTATGG CAAAAATAAA ATGAGCAAGA GCCTTGATAA TGCAATTTTT TTAAACGACG ATGAAAATTT ATTAGAAAAA AAAATTATGT CTATGTATAC AGATCCAAAT AGGATAAGGG CAGATATTCC TGGCAATGTT GAAGGTAATC CCATTTTTAT TTATCACAGT TTTTTTAATA GTAATCATGA AGAAGTTGAG GATCTTAAAA GTAGGTATAA GAAGGGTAAA GTAGGTGATG TTGAGGTTAA GAAAAAATTG TTTTTAGCTT TGAATAGTTT TTTAAAGCCA ATAAGAGATA AAAGAAGTTT TTATGAAGCC AAAAAAAAAG ATTACATTGA TGAAATTATT TTTGATGGTA CAAGCAAGGC GAGGTTTATT GCAAATAAAG TAGTAAAAGA TGTTAAGGAT TTAATAGGGC TTTCAAAAAC TTGGAATGGA ATAAAATATA GCGCTGAGAA AAAATTAAAA AATGAAGAA
Details for BobuA.00743.a.A1.GE26404
HARVESTED ON: 4/9/2009
SEQUENCED ON: 5/8/2009
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MLILKRKVML TGDRPTGALH LGHYVGSVVN RLKFQEEYET YFIIADLHTL TTKPDLKSIN TIPSNVREMV LDYLACGINP DKVSIYLQSA IPELFELHLI FSMIVTVARL QRIPSIKDMS IAAGLKEIPY GLLGYPVLMS ADILMTKANL VPVGRDNESH IEFARELARR FNHLYKNNFF PIPESVFTDS RPLVGIYGKN KMSKSLDNAI FLNDDENLLE KKIMSMYTDP NRIRADIPGN VEGNPVFIYH SFFNSNHEEV EDLKSRYKKG KVGDVEVKKK LFLALNSFLK PIRDKRSFYE AKKKDYIDEI IFDGTSKARF IANKVVKDVK DLIGLSKTWN GIKYSAEKKL KN
Validated NT Sequence
cattttttaa ttttttctca gcgctatatt ttattccatt ccaagttttt gaaagcccta ttaaatcctt aacatctttt actactttat ttgcaataaa cctcgccttg cttgtaccat caaaaataat ttcatcaatg taatcttttt ttttggcttc ataaaaactt cttttatctc ttattggctt taaaaaacta ttcaaagcta aaaacaattt tttcttaacc tcaacatcac ctactttacc cttcttatac ctacttttaa gatcctcaac ttcttcatga ttactattaa aaaaactgtg ataaataaaa acgggattac cttcaacatt gccaggaata tctgccctta tcctatttgg atctgtatac atagacataa tttttttttc taataaattt tcatcgtcgt ttaaaaaaat tgcattatca aggctcttgc tcattttatt tttgccataa atacccacca aaggacgaga atctgtgaaa acagattcgg gtattgggaa aaaattattt ttgtacaaat ggttaaacct tcgtgcaagc tctcttgcaa attcaatatg agattcatta tcacgcccaa cgggaactaa atttgctttt gtcattaaaa tatctgcact cataagaaca ggatacccca aaaggccata aggaatctct ttaagtccag ctgcaatgct catgtctttt atacttggaa tcctttgcaa gcgcgcaaca gtaacaatca ttgaaaatat taaatgaagc tcaaaaagct cgggtatggc tgattgcaga tatatactaa ccttatcagg attaattcca caagcaagat aatctaaaac catttctcta acattagaag gtattgtatt gatgctcttc aagtcaggct ttgtagtcaa agtatgtaaa tcggctataa taaaatatgt ttcatactct tcttgaaatt ttaaccgatt tactacagac cccacatagt gccccaaatg aagagcgcca gtaggcctat ctcccgtaag cataaccttt cttttcaaaa tcaacatcga accaggaccc tgggtctgag cttccagggt a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLILKRKVM LTGDRPTGAL HLGHYVGSVV NRLKFQEEYE TYFIIADLHT LTTKPDLKSI NTIPSNVREM VLDYLACGIN PDKVSIYLQS AIPELFELHL IFSMIVTVAR LQRIPSIKDM SIAAGLKEIP YGLLGYPVLM SADILMTKAN LVPVGRDNES HIEFARELAR RFNHLYKNNF FPIPESVFTD SRPLVGIYGK NKMSKSLDNA IFLNDDENLL EKKIMSMYTD PNRIRADIPG NVEGNPIFIY HSFFNSNHEE VEDLKSRYKK GKVGDVEVKK KLFLALNSFL KPIRDKRSFY EAKKKDYIDE IIFDGTSKAR FIANKVVKDV KDLIGLSKTW NGIKYSAEKK LKNEE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgattttg aaaagaaagg ttatgcttac gggagatagg cctactggcg ctcttcattt ggggcactat gtggggtctg tagtaaatcg gttaaaattt caagaagagt atgaaacata ttttattata gccgatttac atactttgac tacaaagcct gacttgaaga gcatcaatac aataccttct aatgttagag aaatggtttt agattatctt gcttgtggaa ttaatcctga taaggttagt atatatctgc aatcagccat acccgagctt tttgagcttc atttaatatt ttcaatgatt gttactgttg cgcgcttgca aaggattcca agtataaaag acatgagcat tgcagctgga cttaaagaga ttccttatgg ccttttgggg tatcctgttc ttatgagtgc agatatttta atgacaaaag caaatttagt tcccgttggg cgtgataatg aatctcatat tgaatttgca agagagcttg cacgaaggtt taaccatttg tacaaaaata attttttccc aatacccgaa tctgttttca cagattctcg tcctttggtg ggtatttatg gcaaaaataa aatgagcaag agccttgata atgcaatttt tttaaacgac gatgaaaatt tattagaaaa aaaaattatg tctatgtata cagatccaaa taggataagg gcagatattc ctggcaatgt tgaaggtaat cccattttta tttatcacag tttttttaat agtaatcatg aagaagttga ggatcttaaa agtaggtata agaagggtaa agtaggtgat gttgaggtta agaaaaaatt gtttttagct ttgaatagtt ttttaaagcc aataagagat aaaagaagtt tttatgaagc caaaaaaaaa gattacattg atgaaattat ttttgatggt acaagcaagg cgaggtttat tgcaaataaa gtagtaaaag atgttaagga tttaataggg ctttcaaaaa cttggaatgg aataaaatat agcgctgaga aaaaattaaa aaatgaagaa tgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BobuA.00743.a.B1.GE27142
HARVESTED ON: 8/17/2009
SEQUENCED ON: 10/20/2009
EXPECTED MW: 41kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MLTGDRPTGA LHLGHYVGSV VNRLKFQEEY ETYFIIADLH TLTTKPDLKS INTIPSNVRE MVLDYLACGI NPDKVSIYLQ SAIPELFELH LIFSMIVTVA RLQRIPSIKD MSIAAGLKEI PYGLLGYPVL MSADILMTKA NLVPVGRDNE SHIEFARELA RRFNHLYKNN FFPIPESVFT DSRPLVGIYG KNKMSKSLDN AIFLNDDENL LEKKIMSMYT DPNRIRADIP GNVEGNPVFI YHSFFNSNHE EVEDLKSRYK KGKVGDVEVK KKLFLALNSF LKPIRDKRSF YEAKKKDYID EIIFDGTSKA RFIANKVVKD VKDLIGLSKT WNGIKYS
Validated NT Sequence
ggctcaccac caccaccacc accntnngtt gattttgaaa agaaaggtta tgcttacggg agataggcct actggcgctc ttcatttggg gcactatgtg gggtctgtag taaatcggtt aaaatttcaa gaagagtatg aaacatattt tattatagcc gatttacata ctttgactac aaagcctgac ttgaagagca tcaatacaat accttctaat gttagagaaa tggttttaga ttatcttgct tgtggaatta atcctgataa ggttagtata tatctgcaat cagccatacc cgagcttttt gagcttcatt taatattttc aatgattgtt actgttgcgc gcttgcaaag gattccaagt ataaaagaca tgagcattgc agctggactt aaagagattc cttatggcct tttggggtat cctgttctta tgagtgcaga tattttaatg acaaaagcaa atttagttcc cgttgggcgt gataatgaat ctcatattga atttgcaaga gagcttgcac gaaggtttaa ccatttgtac aaaaataatt ttttcccaat acccgaatct gttttcacag attctcgtcc tttggtgggt atttatggca aaaataaaat gagcaagagc cttgataatg caattttttt aaacgacgat gaaaatttat tagaaaaaaa aattatgtct atgtatacag atccaaatag gataagggca gatattcctg gcaatgttga aggtaatccc gtttttattt atcacagttt ttttaatagt aatcatgaag aagttgagga tcttaaaagt aggtataaga agggtaaagt aggtgatgtt gaggttaaga aaaaattgtt tttagctttg aatagttttt taaagccaat aagagataaa agaagttttt atgaagccaa aaaaaaagat tacattgatg aaattatttt tgatggtaca agcaaggcga ggtttattgc aaataaagta gtaaaagatg ttaaggattt aatagggctt tcaaaaactt ggaatggaat aaaatatagc g
Expected Protein Sequence
MAHHHHHHML ILKRKVMLTG DRPTGALHLG HYVGSVVNRL KFQEEYETYF IIADLHTLTT KPDLKSINTI PSNVREMVLD YLACGINPDK VSIYLQSAIP ELFELHLIFS MIVTVARLQR IPSIKDMSIA AGLKEIPYGL LGYPVLMSAD ILMTKANLVP VGRDNESHIE FARELARRFN HLYKNNFFPI PESVFTDSRP LVGIYGKNKM SKSLDNAIFL NDDENLLEKK IMSMYTDPNR IRADIPGNVE GNPIFIYHSF FNSNHEEVED LKSRYKKGKV GDVEVKKKLF LALNSFLKPI RDKRSFYEAK KKDYIDEIIF DGTSKARFIA NKVVKDVKDL IGLSKTWNGI KYSAEKKLKN EE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga ttttgaaaag aaaggttatg cttacgggag ataggcctac tggcgctctt catttggggc actatgtggg gtctgtagta aatcggttaa aatttcaaga agagtatgaa acatatttta ttatagccga tttacatact ttgactacaa agcctgactt gaagagcatc aatacaatac cttctaatgt tagagaaatg gttttagatt atcttgcttg tggaattaat cctgataagg ttagtatata tctgcaatca gccatacccg agctttttga gcttcattta atattttcaa tgattgttac tgttgcgcgc ttgcaaagga ttccaagtat aaaagacatg agcattgcag ctggacttaa agagattcct tatggccttt tggggtatcc tgttcttatg agtgcagata ttttaatgac aaaagcaaat ttagttcccg ttgggcgtga taatgaatct catattgaat ttgcaagaga gcttgcacga aggtttaacc atttgtacaa aaataatttt ttcccaatac ccgaatctgt tttcacagat tctcgtcctt tggtgggtat ttatggcaaa aataaaatga gcaagagcct tgataatgca atttttttaa acgacgatga aaatttatta gaaaaaaaaa ttatgtctat gtatacagat ccaaatagga taagggcaga tattcctggc aatgttgaag gtaatcccat ttttatttat cacagttttt ttaatagtaa tcatgaagaa gttgaggatc ttaaaagtag gtataagaag ggtaaagtag gtgatgttga ggttaagaaa aaattgtttt tagctttgaa tagtttttta aagccaataa gagataaaag aagtttttat gaagccaaaa aaaaagatta cattgatgaa attatttttg atggtacaag caaggcgagg tttattgcaa ataaagtagt aaaagatgtt aaggatttaa tagggctttc aaaaacttgg aatggaataa aatatagcgc tgagaaaaaa ttaaaaaatg aagaatgatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat