SSGCID: Target Page

BobuA.00710.a

coenzyme A disulfide reductase (BB_0728)

CENTER ID:	BobuA.00710.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00710.a.A1.GE26283	Full length( BobuA.00710.a )		1	444

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00710.a.A1.PS00258	Full length( BobuA.00710.a )		1	444

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126784
PATRIC ID:	fig\|224326.49.peg.1119
RefSeq:	NP_212862.1
UniProt:	O51670

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMKIIIIGGT SAGTSAAAKA NRLNKKLDIT IYEKTNIVSF GTCGLPYFVG GFFDNPNTMI SRTQEEFEKT GISVKTNHEV IKVDAKNNTI VIKNQKTGTI FNNTYDQLMI ATGAKPIIPP INNINLENFH TLKNLEDGQK IKKLMDREEI KNIVIIGGGY IGIEMVEAAK NKRKNVRLIQ LDKHILIDSF DEEIVTIMEE ELTKKGVNLH TNEFVKSLIG EKKAEGVVTN KNTYQADAVI LATGIKPDTE FLENQLKTTK NGAIIVNEYG ETSIKNIFSA GDCATIYNIV SKKNEYIPLA TTANKLGRIV GENLAGNHTA FKGTLGSASI KILSLEAART GLTEKDAKKL QIKYKTIFVK DKNHTNYYPG QEDLYIKLIY EENTKIILGA QAIGKNGAVI RIHALSIAIY SKLTTKELGM MDFSYSPPFS RTWDILNIAG NAAK

NT Sequence

ATGATGAAAA TAATAATTAT TGGGGGCACA TCAGCAGGAA CTAGTGCCGC AGCTAAAGCA AACCGCTTAA ACAAAAAGCT AGACATTACT ATCTATGAAA AAACAAATAT TGTATCTTTT GGAACCTGTG GCCTGCCTTA CTTTGTGGGG GGATTCTTTG ACAACCCCAA TACAATGATC TCAAGAACAC AAGAAGAATT CGAAAAAACT GGAATCTCTG TTAAAACTAA CCACGAAGTT ATCAAAGTAG ATGCAAAAAA CAATACAATT GTAATAAAAA ATCAAAAAAC AGGAACCATT TTTAACAATA CTTACGATCA ACTTATGATA GCAACTGGTG CAAAACCTAT TATTCCACCA ATCAATAATA TCAATCTAGA AAATTTTCAT ACTCTGAAAA ATTTAGAAGA CGGTCAAAAA ATAAAAAAAT TAATGGATAG AGAAGAGATT AAAAATATAG TGATAATTGG TGGTGGATAC ATTGGAATTG AAATGGTAGA AGCAGCAAAA AATAAAAGAA AAAATGTAAG ATTAATTCAA CTAGATAAGC ACATACTCAT AGATTCCTTT GACGAAGAAA TAGTCACAAT AATGGAAGAA GAACTAACAA AAAAGGGGGT TAATCTTCAT ACAAATGAGT TTGTAAAAAG TTTAATAGGA GAAAAAAAGG CAGAAGGAGT AGTAACAAAC AAAAATACTT ATCAAGCTGA CGCTGTTATA CTTGCTACCG GAATAAAACC TGACACTGAA TTTTTAGAAA ACCAGCTTAA AACTACTAAA AATGGAGCAA TAATTGTAAA TGAGTATGGC GAAACTAGCA TAAAAAATAT TTTTTCTGCA GGAGATTGTG CAACTATTTA TAATATAGTA AGTAAAAAAA ATGAATACAT ACCCTTGGCA ACAACAGCCA ACAAACTTGG AAGAATAGTT GGTGAAAATT TAGCTGGGAA TCATACAGCA TTTAAAGGCA CATTGGGCTC AGCTTCAATT AAAATACTAT CTTTAGAAGC TGCAAGAACA GGACTTACAG AAAAAGATGC AAAAAAGCTC CAAATAAAAT ATAAAACGAT TTTTGTAAAG GACAAAAATC ATACAAATTA TTATCCAGGC CAAGAAGATC TTTATATTAA ATTAATTTAT GAGGAAAATA CCAAAATAAT CCTTGGGGCA CAAGCAATAG GAAAAAATGG AGCCGTAATA AGAATTCATG CTTTATCAAT TGCAATCTAT TCAAAACTTA CAACAAAAGA GCTAGGGATG ATGGATTTCT CATATTCCCC ACCCTTCTCA AGAACTTGGG ATATATTAAA TATTGCTGGC AATGCTGCCA AA

Details for BobuA.00710.a.A1.GE26283

HARVESTED ON:	3/2/2009
SEQUENCED ON:	7/12/2017
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMMKIIIIGG TSAGTSAAAK ANRLNKKLDI TIYEKTNIVS FGTCGLPYFV GGFFDNPNTM ISRTQEEFEK TGISVKTNHE VIKVDAKNNT IVIKNQKTGT IFNNTYDQLM IATGAKPIIP PINNINLENF HTLKNLEDGQ KIKKLMDREE IKNIVIIGGG YIGIEMVEAA KNKRKNVRLI QLDKHILIDS FDEEIVTIME EELTKKGVNL HTNEFVKSLI GEKKAEGVVT NKNTYQADAV ILATGIKPDT EFLENQLKTT KNGAIIVNEY GETSIKNIFS AGDCATIYNI VSKKNEYIPL ATTANKLGRI VGENLAGNHT AFKGTLGSAS IKILSLEAAR TGLTEKDAKK LQIKYKTIFV KDKNHTNYYP GQEDLYIKLI YEENTKIILG AQAIGKNGAV IRIHALSIAI YSKLTTKELG MMDFSYSPPF SRTWDILNIA GNAAK

Validated NT Sequence

ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgatgaaa ataataatta ttgggggcac atcagcagga actagtgccg cagctaaagc aaaccgctta aacaaaaagc tagacattac tatctatgaa aaaacaaata ttgtatcttt tggaacctgt ggcctgcctt actttgtggg gggattcttt gacaacccca atacaatgat ctcaagaaca caagaagaat tcgaaaaaac tggaatctct gttaaaacta accacgaagt tatcaaagta gatgcaaaaa acaatacaat tgtaataaaa aatcaaaaaa caggaaccat ttttaacaat acttacgatc aacttatgat agcaactggt gcaaaaccta ttattccacc aatcaataat atcaatctag aaaattttca tactctgaaa aatttagaag acggtcaaaa aataaaaaaa ttaatggata gagaagagat taaaaatata gtgataattg gtggtggata cattggaatt gaaatggtag aagcagcaaa aaataaaaga aaaaatgtaa gattaattca actagataag cacatactca tagattcctt tgacgaagaa atagtcacaa taatggaaga agaactaaca aaaaaggggg ttaatcttca tacaaatgag tttgtaaaaa gtttaatagg agaaaaaaag gcagaaggag tagtaacaaa caaaaatact tatcaagctg acgctgttat acttgctacc ggaataaaac ctgacactga atttttagaa aaccagctta aaactactaa aaatggagca ataattgtaa atgagtatgg cgaaactagc ataaaaaata ttttttctgc aggagattgt gcaactattt ataatatagt aagtaaaaaa aatgaataca tacccttggc aacaacagcc aacaaacttg gaagaatagt tggtgaaaat ttagctggga atcatacagc atttaaaggc acattgggct cagcttcaat taaaatacta tctttagaag ctgcaagaac aggacttaca gaaaaagatg caaaaaagct ccaaataaaa tataaaacga tttttgtaaa ggacaaaaat catacaaatt attatccagg ccaagaagat ctttatatta aattaattta tgaggaaaat accaaaataa tccttggggc acaagcaata ggaaaaaatg gagccgtaat aagaattcat gctttatcaa ttgcaatcta ttcaaaactt acaacaaaag agctagggat gatggatttc tcatattccc cacccttctc aagaacttgg gatatattaa atattgctgg caatgctgcc aaataataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgaaaata ataattattg ggggcacatc agcaggaact agtgccgcag ctaaagcaaa ccgcttaaac aaaaagctag acattactat ctatgaaaaa acaaatattg tatcttttgg aacctgtggc ctgccttact ttgtgggggg attctttgac aaccccaata caatgatctc aagaacacaa gaagaattcg aaaaaactgg aatctctgtt aaaactaacc acgaagttat caaagtagat gcaaaaaaca atacaattgt aataaaaaat caaaaaacag gaaccatttt taacaatact tacgatcaac ttatgatagc aactggtgca aaacctatta ttccaccaat caataatatc aatctagaaa attttcatac tctgaaaaat ttagaagacg gtcaaaaaat aaaaaaatta atggatagag aagagattaa aaatatagtg ataattggtg gtggatacat tggaattgaa atggtagaag cagcaaaaaa taaaagaaaa aatgtaagat taattcaact agataagcac atactcatag attcctttga cgaagaaata gtcacaataa tggaagaaga actaacaaaa aagggggtta atcttcatac aaatgagttt gtaaaaagtt taataggaga aaaaaaggca gaaggagtag taacaaacaa aaatacttat caagctgacg ctgttatact tgctaccgga ataaaacctg acactgaatt tttagaaaac cagcttaaaa ctactaaaaa tggagcaata attgtaaatg agtatggcga aactagcata aaaaatattt tttctgcagg agattgtgca actatttata atatagtaag taaaaaaaat gaatacatac ccttggcaac aacagccaac aaacttggaa gaatagttgg tgaaaattta gctgggaatc atacagcatt taaaggcaca ttgggctcag cttcaattaa aatactatct ttagaagctg caagaacagg acttacagaa aaagatgcaa aaaagctcca aataaaatat aaaacgattt ttgtaaagga caaaaatcat acaaattatt atccaggcca agaagatctt tatattaaat taatttatga ggaaaatacc aaaataatcc ttggggcaca agcaatagga aaaaatggag ccgtaataag aattcatgct ttatcaattg caatctattc aaaacttaca acaaaagagc tagggatgat ggatttctca tattccccac ccttctcaag aacttgggat atattaaata ttgctggcaa tgctgccaaa tagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BobuA.00710.a.A1.PS00258

PURIFICATION DATe:	5/13/2009
CONCENTRATION:	52.65mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGAAAATA ATAATTATTG GGGGCACATC AGCAGGAACT AGTGCCGCAG CTAAAGCAAA CCGCTTAAAC AAAAAGCTAG ACATTACTAT CTATGAAAAA ACAAATATTG TATCTTTTGG AACCTGTGGC CTGCCTTACT TTGTGGGGGG ATTCTTTGAC AACCCCAATA CAATGATCTC AAGAACACAA GAAGAATTCG AAAAAACTGG AATCTCTGTT AAAACTAACC ACGAAGTTAT CAAAGTAGAT GCAAAAAACA ATACAATTGT AATAAAAAAT CAAAAAACAG GAACCATTTT TAACAATACT TACGATCAAC TTATGATAGC AACTGGTGCA AAACCTATTA TTCCACCAAT CAATAATATC AATCTAGAAA ATTTTCATAC TCTGAAAAAT TTAGAAGACG GTCAAAAAAT AAAAAAATTA ATGGATAGAG AAGAGATTAA AAATATAGTG ATAATTGGTG GTGGATACAT TGGAATTGAA ATGGTAGAAG CAGCAAAAAA TAAAAGAAAA AATGTAAGAT TAATTCAACT AGATAAGCAC ATACTCATAG ATTCCTTTGA CGAAGAAATA GTCACAATAA TGGAAGAAGA ACTAACAAAA AAGGGGGTTA ATCTTCATAC AAATGAGTTT GTAAAAAGTT TAATAGGAGA AAAAAAGGCA GAAGGAGTAG TAACAAACAA AAATACTTAT CAAGCTGACG CTGTTATACT TGCTACCGGA ATAAAACCTG ACACTGAATT TTTAGAAAAC CAGCTTAAAA CTACTAAAAA TGGAGCAATA ATTGTAAATG AGTATGGCGA AACTAGCATA AAAAATATTT TTTCTGCAGG AGATTGTGCA ACTATTTATA ATATAGTAAG TAAAAAAAAT GAATACATAC CCTTGGCAAC AACAGCCAAC AAACTTGGAA GAATAGTTGG TGAAAATTTA GCTGGGAATC ATACAGCATT TAAAGGCACA TTGGGCTCAG CTTCAATTAA AATACTATCT TTAGAAGCTG CAAGAACAGG ACTTACAGAA AAAGATGCAA AAAAGCTCCA AATAAAATAT AAAACGATTT TTGTAAAGGA CAAAAATCAT ACAAATTATT ATCCAGGCCA AGAAGATCTT TATATTAAAT TAATTTATGA GGAAAATACC AAAATAATCC TTGGGGCACA AGCAATAGGA AAAAATGGAG CCGTAATAAG AATTCATGCT TTATCAATTG CAATCTATTC AAAACTTACA ACAAAAGAGC TAGGGATGAT GGATTTCTCA TATTCCCCAC CCTTCTCAAG AACTTGGGAT ATATTAAATA TTGCTGGCAA TGCTGCCAAA TAGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA