BobuA.00667.a

hypothetical protein

CENTER ID:	BobuA.00667.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00667.a.A1.GE26279	Full length( BobuA.00667.a )		1	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_128305
PATRIC ID:	fig\|224326.49.peg.527
RefSeq:	NP_212263.1
UniProt:	O51155

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIALKAPRVH VFLAEKGVGS RRFCEELIRK KLVRVNNTIA KLGDKVTLGD RIIYKKQIFV FKDFQINNRI YLALNKPRNY LCSNFDVDGR KLAISLVQPL FKERVFSIGR LDFKSSGLLL FTNDGKFAND IIHPRQKVER EYIIESKKDI DENLLISFKS GIKVKKEFFK LKSYEILNKN SARLILDEGK NREIRKVFLS KNIFLKKIHR IRIGNINLDS LKEGQVKIVP LSKINSLKSR LEKLNDNSN

NT Sequence

ATGATTGCAC TTAAAGCCCC TAGAGTTCAC GTTTTTTTAG CTGAAAAGGG AGTGGGGTCA AGAAGATTTT GCGAGGAACT GATAAGAAAG AAACTTGTAA GAGTTAATAA CACTATTGCT AAGCTTGGGG ATAAAGTAAC TTTAGGAGAC AGAATAATTT ATAAAAAACA GATTTTTGTT TTTAAAGATT TTCAAATTAA TAATAGAATT TATCTAGCTC TTAACAAGCC TAGAAATTAT TTATGTTCTA ATTTTGATGT TGATGGAAGA AAGTTAGCAA TATCTTTGGT TCAGCCCTTA TTTAAAGAGC GTGTGTTTTC AATTGGTAGG CTTGATTTTA AAAGCTCTGG ACTTTTATTA TTCACCAATG ATGGTAAATT TGCAAACGAT ATTATTCATC CAAGGCAAAA AGTTGAAAGA GAATATATTA TTGAATCAAA AAAAGATATT GATGAAAATT TGCTTATTTC TTTTAAATCG GGTATAAAGG TAAAAAAAGA ATTTTTTAAA TTAAAATCTT ATGAAATTTT AAATAAAAAT TCTGCTAGAT TGATTTTAGA TGAAGGGAAA AATAGAGAAA TAAGAAAAGT GTTTTTGAGC AAGAATATTT TTTTAAAAAA AATTCATAGA ATTAGAATTG GCAATATTAA TTTAGATAGT TTAAAGGAGG GTCAAGTAAA AATTGTTCCT TTGTCTAAGA TAAATAGTTT AAAATCCAGG CTGGAGAAAT TAAATGATAA TAGCAAT

Details for BobuA.00667.a.A1.GE26279

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/30/2009
EXPECTED MW:	31kDa
OBSERVED MW:	16kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	failed alignment
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIALKAPRV HVFLAEKGVG SRRFCEELIR KKLVRVNNTI AKLGDKVTLG DRIIYKKQIF VFKDFQINNR IYLALNKPRN YLCSNFDVDG RKLAISLVQP LFKERVFSIG RLDFKSSGLL LFTNDGKFAN DIIHPRQKVE REYIIESKKD IDENLLISFK SGIKVKKEFF KLKSYEILNK NSARLILDEG KNREIRKVFL SKNIFLKKIH RIRIGNINLD SLKEGQVKIV PLSKINSLKS RLEKLNDNSN

Validated NT Sequence

ttattaattg ctattatcat ttaatttctc cagcctggat tttaaactat ttatcttaga caaaggaaca atttttactt gaccctcctt taaactatct aaattaatat tgccaattct aattctatga atttttttta aaaaaatatt cttgctcaaa aacacttttc ttatttctct atttttccct tcatctaaaa tcaatctagc agaattttta tttaaaattt cataagattt taatttaaaa aattcttttt ttacctttat acccgattta aaagaaataa gcaaattttc atcaatatct ttttttgatt caataatata ttctctttca actttttgcc ttggatgaat aatatcgttt gcaaatttac catcattggt gaataataaa agtccagagc ttttaaaatc aagcctacca attgaaaaca cacgctcttt aaataagggc tgaaccaaag atattgctaa ctttcttcca tcaacatcaa aattagaaca taaataattt ctaggcttgt taagagctag ataaattcta ttattaattt gaaaatcttt aaaaacaaaa atctgttttt tataaattat tctgtctcct aaagttactt tatccccaag cttagcaata gtgttattaa ctcttacaag tttctttctt atcagttcct cgcaaaatct tcttgacccc actccctttt cagctaaaaa aacgtgaact ctaggggctt taagtgcaat catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattgcactt aaagccccta gagttcacgt ttttttagct gaaaagggag tggggtcaag aagattttgc gaggaactga taagaaagaa acttgtaaga gttaataaca ctattgctaa gcttggggat aaagtaactt taggagacag aataatttat aaaaaacaga tttttgtttt taaagatttt caaattaata atagaattta tctagctctt aacaagccta gaaattattt atgttctaat tttgatgttg atggaagaaa gttagcaata tctttggttc agcccttatt taaagagcgt gtgttttcaa ttggtaggct tgattttaaa agctctggac ttttattatt caccaatgat ggtaaatttg caaacgatat tattcatcca aggcaaaaag ttgaaagaga atatattatt gaatcaaaaa aagatattga tgaaaatttg cttatttctt ttaaatcggg tataaaggta aaaaaagaat tttttaaatt aaaatcttat gaaattttaa ataaaaattc tgctagattg attttagatg aagggaaaaa tagagaaata agaaaagtgt ttttgagcaa gaatattttt ttaaaaaaaa ttcatagaat tagaattggc aatattaatt tagatagttt aaaggagggt caagtaaaaa ttgttccttt gtctaagata aatagtttaa aatccaggct ggagaaatta aatgataata gcaattgaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa