BobuA.00645.a

hypothetical protein (BB_0099)

CENTER ID: BobuA.00645.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00645.a.A1.GE26275 Full length( BobuA.00645.a ) 1 307
BobuA.00645.a.B1.GE27140 Full length( BobuA.00645.a ) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_132289
PATRIC ID: fig|224326.49.peg.497
RefSeq: NP_212233.1
UniProt: O51126
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNYLEFEVIW GVNNIYSILE LNSKLIYEGI FKGKVLETGC KEYSPLVPGD IVLGYIYSSR KVYIDKRLSR KNILWRYNKK ADLRQTIVSN IDNVLIVSSA NFPEMKNFFI DRVLIVAEEQ NIVPIIVINK IDKGISQKAQ EFSEIYENLG YKVLKTSVKT YEGIKEVKEV LKNSRTSFIG QSGVGKSSLI NLIDSRASQS VNEISHKYSR GKHTTVYSIS FHSDSGIIVD TPGIKEFGIE TLPFENLKYY FKEFENFASF CKYKSCLHVS EPYCSVTSSL DNGISKLRYE SYLKILSELK NYKNYAR
NT Sequence
TTGAATTATC TTGAATTTGA AGTTATTTGG GGAGTTAATA ATATATATTC TATTTTAGAG CTTAACAGTA AGTTAATTTA TGAAGGAATT TTTAAGGGTA AGGTATTAGA AACAGGTTGC AAGGAGTACA GTCCTTTAGT TCCGGGGGAT ATAGTCTTAG GATATATTTA TAGTTCTCGC AAAGTGTATA TTGATAAGCG ATTAAGCAGG AAAAATATTC TTTGGCGTTA CAATAAGAAA GCAGATCTTA GACAGACAAT TGTTTCTAAT ATTGATAATG TTTTGATTGT AAGTTCTGCT AATTTTCCTG AGATGAAAAA TTTCTTTATT GACAGAGTTC TTATTGTTGC AGAAGAACAA AATATTGTTC CTATTATTGT GATTAATAAA ATTGACAAAG GAATAAGCCA AAAGGCACAA GAATTTTCTG AAATTTATGA AAATCTAGGA TACAAGGTTT TAAAAACTTC TGTTAAAACT TATGAGGGCA TTAAAGAAGT AAAAGAGGTT TTGAAAAATT CAAGAACTTC TTTTATTGGC CAGTCTGGCG TTGGTAAATC TTCTTTGATA AATTTGATTG ATTCAAGAGC TTCTCAATCG GTAAATGAAA TTTCACATAA GTATTCTAGG GGAAAACATA CTACCGTTTA TTCAATATCA TTTCATTCTG ATAGCGGAAT AATAGTTGAT ACTCCAGGAA TAAAGGAGTT TGGAATTGAA ACATTACCCT TTGAGAATCT TAAGTATTAT TTTAAAGAGT TTGAAAATTT TGCTAGTTTT TGTAAATATA AATCCTGTTT GCATGTTAGC GAACCTTATT GTTCTGTTAC AAGTTCTTTG GATAATGGAA TTTCTAAACT TAGATACGAA AGCTATTTAA AGATTTTGTC AGAGCTTAAA AATTATAAAA ATTATGCAAG A
Details for BobuA.00645.a.A1.GE26275
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/6/2009
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMLNYLEF EVIWGVNNIY SILELNSKLI YEGIFKGKVL ETGCKEYSPL VPGDIVLGYI YSSRKVYIDK RLSRKNILWR YNKKADLRQT IVSNIDNVLI VSSANFPEMK NFFIDRVLIV AEEQNIVPII VINKIDKGIS QKAQEFSEIY ENLGYKVLKT SVKTYEGIKE VKEVLKNSRT SFIGQSGVGK SSLINLIDSR ASQSVNEISH KYSRGKHTTV YSISFHSDSG IIVDTPGIKE FGIETLPFEN LKYYFKEFEN FASFCKYKSC LHVSEPYCSV TSSLDNGISK LRYESYLKIL SELKNYKNYA R
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgaa ttatcttgaa tttgaagtta tttggggagt taataatata tattctattt tagagcttaa cagtaagtta atttatgaag gaatttttaa gggtaaggta ttagaaacag gttgcaagga gtacagtcct ttagttccgg gggatatagt cttaggatat atttatagtt ctcgcaaagt gtatattgat aagcgattaa gcaggaaaaa tattctttgg cgttacaata agaaagcaga tcttagacag acaattgttt ctaatattga taatgttttg attgtaagtt ctgctaattt tcctgagatg aaaaatttct ttattgacag agttcttatt gttgcagaag aacaaaatat tgttcctatt attgtgatta ataaaattga caaaggaata agccaaaagg cacaagaatt ttctgaaatt tatgaaaatc taggatacaa ggttttaaaa acttctgtta aaacttatga gggcattaaa gaagtaaaag aggttttgaa aaattcaaga acttctttta ttggccagtc tggcgttggt aaatcttctt tgataaattt gattgattca agagcttctc aatcggtaaa tgaaatttca cataagtatt ctaggggaaa acatactacc gtttattcaa tatcatttca ttctgatagc ggaataatag ttgatactcc aggaataaag gagtttggaa ttgaaacatt accctttgag aatcttaagt attattttaa agagtttgaa aattttgcta gtttttgtaa atataaatcc tgtttgcatg ttagcgaacc ttattgttct gttacaagtt ctttggataa tggaatttct aaacttagat acgaaagcta tttaaagatt ttgtcagagc ttaaaaatta taaaaattat gcaagataat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLNYLEFEV IWGVNNIYSI LELNSKLIYE GIFKGKVLET GCKEYSPLVP GDIVLGYIYS SRKVYIDKRL SRKNILWRYN KKADLRQTIV SNIDNVLIVS SANFPEMKNF FIDRVLIVAE EQNIVPIIVI NKIDKGISQK AQEFSEIYEN LGYKVLKTSV KTYEGIKEVK EVLKNSRTSF IGQSGVGKSS LINLIDSRAS QSVNEISHKY SRGKHTTVYS ISFHSDSGII VDTPGIKEFG IETLPFENLK YYFKEFENFA SFCKYKSCLH VSEPYCSVTS SLDNGISKLR YESYLKILSE LKNYKNYAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaattat cttgaatttg aagttatttg gggagttaat aatatatatt ctattttaga gcttaacagt aagttaattt atgaaggaat ttttaagggt aaggtattag aaacaggttg caaggagtac agtcctttag ttccggggga tatagtctta ggatatattt atagttctcg caaagtgtat attgataagc gattaagcag gaaaaatatt ctttggcgtt acaataagaa agcagatctt agacagacaa ttgtttctaa tattgataat gttttgattg taagttctgc taattttcct gagatgaaaa atttctttat tgacagagtt cttattgttg cagaagaaca aaatattgtt cctattattg tgattaataa aattgacaaa ggaataagcc aaaaggcaca agaattttct gaaatttatg aaaatctagg atacaaggtt ttaaaaactt ctgttaaaac ttatgagggc attaaagaag taaaagaggt tttgaaaaat tcaagaactt cttttattgg ccagtctggc gttggtaaat cttctttgat aaatttgatt gattcaagag cttctcaatc ggtaaatgaa atttcacata agtattctag gggaaaacat actaccgttt attcaatatc atttcattct gatagcggaa taatagttga tactccagga ataaaggagt ttggaattga aacattaccc tttgagaatc ttaagtatta ttttaaagag tttgaaaatt ttgctagttt ttgtaaatat aaatcctgtt tgcatgttag cgaaccttat tgttctgtta caagttcttt ggataatgga atttctaaac ttagatacga aagctattta aagattttgt cagagcttaa aaattataaa aattatgcaa gatgaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BobuA.00645.a.B1.GE27140
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML NYLEFEVIWG VNNIYSILEL NSKLIYEGIF KGKVLETGCK EYSPLVPGDI VLGYIYSSRK VYIDKRLSRK NILWRYNKKA DLRQTIVSNI DNVLIVSSAN FPEMKNFFID RVLIVAEEQN IVPIIVINKI DKGISQKAQE FSEIYENLGY KVLKTSVKTY EGIKEVKEVL KNSRTSFIGQ SGVGKSSLIN LIDSRASQSV NEISHKYSRG KHTTVYSISF HSDSGIIVDT PGIKEFGIET LPFENLKYYF KEFENFASFC KYKSCLHVSE PYCSVTSSLD NGISKLRYES YLKILSELKN YKNYAR
Validated NT Sequence
actcacttat cttgcataat ttttataatt tttaagctct gacaaaatct ttaaatagct ttcgtatcta agtttagaaa ttccattatc caaagaactt gtaacagaac aataaggttc gctaacatgc aaacaggatt tatatttaca aaaactagca aaattttcaa actctttaaa ataatactta agattctcaa agggtaatgt ttcaattcca aactccttta ttcctggagt atcaactatt attccgctat cagaatgaaa tgatattgaa taaacggtag tatgttttcc cctagaatac ttatgtgaaa tttcatttac cgattgagaa gctcttgaat caatcaaatt tatcaaagaa gatttaccaa cgccagactg gccaataaaa gaagttcttg aatttttcaa aacctctttt acttctttaa tgccctcata agttttaaca gaagttttta aaaccttgta tcctagattt tcataaattt cagaaaattc ttgtgccttt tggcttattc ctttgtcaat tttattaatc acaataatag gaacaatatt ttgttcttct gcaacaataa gaactctgtc aataaagaaa tttttcatct caggaaaatt agcagaactt acaatcaaaa cattatcaat attagaaaca attgtctgtc taagatctgc tttcttattg taacgccaaa gaatattttt cctgcttaat cgcttatcaa tatacacttt gcgagaacta taaatatatc ctaagactat atcccccgga actaaaggac tgtactcctt gcaacctgtt tctaatacct tacccttaaa aattccttca taaattaact tactgttaag ctctaaaata gaatatatat tattaactcc ccaaataact tcaaattcaa gataattcaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHML NYLEFEVIWG VNNIYSILEL NSKLIYEGIF KGKVLETGCK EYSPLVPGDI VLGYIYSSRK VYIDKRLSRK NILWRYNKKA DLRQTIVSNI DNVLIVSSAN FPEMKNFFID RVLIVAEEQN IVPIIVINKI DKGISQKAQE FSEIYENLGY KVLKTSVKTY EGIKEVKEVL KNSRTSFIGQ SGVGKSSLIN LIDSRASQSV NEISHKYSRG KHTTVYSISF HSDSGIIVDT PGIKEFGIET LPFENLKYYF KEFENFASFC KYKSCLHVSE PYCSVTSSLD NGISKLRYES YLKILSELKN YKNYAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga attatcttga atttgaagtt atttggggag ttaataatat atattctatt ttagagctta acagtaagtt aatttatgaa ggaattttta agggtaaggt attagaaaca ggttgcaagg agtacagtcc tttagttccg ggggatatag tcttaggata tatttatagt tctcgcaaag tgtatattga taagcgatta agcaggaaaa atattctttg gcgttacaat aagaaagcag atcttagaca gacaattgtt tctaatattg ataatgtttt gattgtaagt tctgctaatt ttcctgagat gaaaaatttc tttattgaca gagttcttat tgttgcagaa gaacaaaata ttgttcctat tattgtgatt aataaaattg acaaaggaat aagccaaaag gcacaagaat tttctgaaat ttatgaaaat ctaggataca aggttttaaa aacttctgtt aaaacttatg agggcattaa agaagtaaaa gaggttttga aaaattcaag aacttctttt attggccagt ctggcgttgg taaatcttct ttgataaatt tgattgattc aagagcttct caatcggtaa atgaaatttc acataagtat tctaggggaa aacatactac cgtttattca atatcatttc attctgatag cggaataata gttgatactc caggaataaa ggagtttgga attgaaacat taccctttga gaatcttaag tattatttta aagagtttga aaattttgct agtttttgta aatataaatc ctgtttgcat gttagcgaac cttattgttc tgttacaagt tctttggata atggaatttc taaacttaga tacgaaagct atttaaagat tttgtcagag cttaaaaatt ataaaaatta tgcaagatga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat