SSGCID: Target Page

BobuA.00629.a

long-chain-fatty-acid CoAligase (BB_0137)

CENTER ID:	BobuA.00629.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00629.a.B2.GE39062	soluble domain #1		1	200

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.535
RefSeq:	NP_212271.1
UniProt:	O51162

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIAKAFFEV ADQQKDKIAQ MYKVCNGYAH VTYGDLKNNV LKLASFLKSI NVKHQDKIFI CSENRAEWTV IDFAILSLGA VDIPKGSDVT LFEAEIIFNS VLPSVVILEN LNLLNMFIQI KFTVKPIFII IENLNQEDRL KYSDFEIYTY SDCILFGDTL RKDSEIIEIA SKVDSNDMAT IIYTSGTTGH PKGVMLSHAN LLYQVSSFSL MVDTHVGQIF MCILPIWHSF QRSFSYNIFL KGMVCLFSTI VPRAMLDDIK NVNPHYIAAV PRLWIAIRQN IYKEVSKKPF ISRMIFHFFV KAAFLSDICY RVVMGLYPDN GFSLFFPIKK ILGIFGLICA FPFKALGNVL IFNKINKILG NNFVVGITGG GSMPLSAVRF FNSIGIELAN AYGLTETSPG VASNKHKKVI IGTCGKILPG TVAEIRDIDG NKLKKPGKGI LFVKGPQVML GYYKDKEATC KIIGSDGFLN TGDIAKLSKD NVVQIIGREK DTIVLNNGEN VEPAPIEIKL EESALIEKAV VVGQDQKFLG ALILPNFEEI NKYLESVGQK IFDANNRRQI IANNIVLKAI NDEIKKLINK ANGFKPFEQI LKFTLLEKPF EVGKEMSIKM DIKRSYILNF YKNEIKNLFI

NT Sequence

atgtcaatag caaaggcatt ttttgaagtg gctgatcaac aaaaagataa aattgctcaa atgtataaag tttgcaatgg atatgcccac gttacttatg gcgacttaaa aaacaatgtt ttaaagcttg cttccttttt aaagtcaatc aatgttaagc atcaagataa aatatttatt tgctctgaga atagggctga gtggactgtg atagattttg caattttatc tttgggtgcc gttgatattc caaagggttc tgatgttact ctttttgaag cggaaattat ttttaactcc gttcttccaa gtgtagttat tttagaaaat ttaaatcttc ttaatatgtt tattcaaatt aaatttacag taaagccaat atttattatt attgaaaatt taaaccaaga ggatagatta aaatatagtg attttgaaat ttatacttat agcgattgta ttttatttgg agatacctta agaaaagatt cagaaattat tgagattgca agtaaagttg attctaatga tatggcaaca ataatatata cttctggaac aacaggccac ccaaaggggg taatgctcag ccatgccaat cttctttatc aggtttctag ttttagttta atggttgata cgcatgtggg acaaatattt atgtgcattt tgccaatttg gcattccttt caaaggtctt tttcttacaa tattttcctc aagggtatgg tttgtttatt ttccaccatt gttccaaggg caatgcttga tgatattaaa aatgttaatc ctcattatat tgcagctgtt cctaggcttt ggattgcaat aagacaaaat atttacaaag aagtttctaa gaaaccgttt atttctagaa tgatttttca tttttttgta aaagcggcat ttcttagcga tatttgctat agggtggtaa tggggcttta tccggacaac gggtttagtt tatttttccc tataaagaaa attttaggaa tatttggatt aatctgtgca tttcctttta aggctttggg gaatgtttta atttttaata aaataaataa gatcttgggc aataattttg ttgttgggat tactggtggc gggagtatgc cattgtctgc tgttagattt tttaattcaa ttggtattga gcttgccaat gcttatggat tgactgaaac ctcgcctgga gtggcttcta ataagcataa aaaagtgatt attgggactt gtggtaaaat tttgcccgga actgttgctg aaattagaga tattgatggg aataagctta aaaagcctgg aaagggaatt ttgtttgtaa aagggcctca agtaatgctt ggatattaca aggacaaaga agcaacttgt aagattattg gttctgatgg gtttttaaac acgggtgata ttgcaaaatt atccaaggat aatgttgttc aaattattgg tcgagaaaaa gatacaattg ttttgaataa tggagagaat gttgagcctg ctccaattga gattaagctt gaagaatcag cattaataga aaaagcagtt gttgtagggc aagatcaaaa attcttaggc gctcttattc ttccaaattt tgaagaaata aataaatatc tagaaagtgt tggacaaaaa atttttgatg ctaataatag gcgtcaaatt attgcgaata atattgttct taaggccata aatgatgaaa taaaaaaatt aatcaataaa gccaatggat ttaagccttt tgagcagata ttaaagttta ctcttttaga aaaaccattt gaagttggta aagaaatgtc tattaaaatg gacattaagc gaagctatat tttaaatttt tataaaaatg aaattaaaaa tttatttatt taa

Details for BobuA.00629.a.B2.GE39062

HARVESTED ON:	3/31/2015
SEQUENCED ON:	3/27/2015
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS IAKAFFEVAD QQKDKIAQMY KVCNGYAHVT YGDLKNNVLK LASFLKSINV KHQDKIFICS ENRAEWTVID FAILSLGAVD IPKGSDVTLF EAEIIFNSVL PSVVILENLN LLNMFIQIKF TVKPIFIIIE NLNQEDRLKY SDFEIYTYSD CILFGDTLRK DSEIIEIASK VDSNDMATII YTSGTTGHPK GVMLSHAN

Validated NT Sequence

atcttactca cttaattggc atggctgagc attaccccct ttgggtggcc tgttgttcca gaagtatata ttattgttgc catatcatta gaatcaactt tacttgcaat ctcaataatt tctgaatctt ttcttaaggt atctccaaat aaaatacaat cgctataagt ataaatttca aaatcactat attttaatct atcctcttgg tttaaatttt caataataat aaatattggc tttactgtaa atttaatttg aataaacata ttaagaagat ttaaattttc taaaataact acacttggaa gaacggagtt aaaaataatt tccgcttcaa aaagagtaac atcagaaccc tttggaatat caacggcacc caaagataaa attgcaaaat ctatcacagt ccactcagcc ctattctcag agcaaataaa tattttatct tgatgcttaa cattgattga ctttaaaaag gaagcaagct ttaaaacatt gttttttaag tcgccataag taacgtgggc atatccattg caaactttat acatttgagc aattttatct ttttgttgat cagccacttc aaaaaatgcc tttgctattg acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa tagcaaaggc attttttgaa gtggctgatc aacaaaaaga taaaattgct caaatgtata aagtttgcaa tggatatgcc cacgttactt atggcgactt aaaaaacaat gttttaaagc ttgcttcctt tttaaagtca atcaatgtta agcatcaaga taaaatattt atttgctctg agaatagggc tgagtggact gtgatagatt ttgcaatttt atctttgggt gccgttgata ttccaaaggg ttctgatgtt actctttttg aagcggaaat tatttttaac tccgttcttc caagtgtagt tattttagaa aatttaaatc ttcttaatat gtttattcaa attaaattta cagtaaagcc aatatttatt attattgaaa atttaaacca agaggataga ttaaaatata gtgattttga aatttatact tatagcgatt gtattttatt tggagatacc ttaagaaaag attcagaaat tattgagatt gcaagtaaag ttgattctaa tgatatggca acaataatat atacttctgg aacaacaggc cacccaaagg gggtaatgct cagccatgcc aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat