BobuA.00616.a

aspartyl/glutamyl-tRNA amidotransferase subunit B (BB_0341)

CENTER ID: BobuA.00616.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00616.a.A1.GE26273 Full length( BobuA.00616.a ) 1 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00616.a.A1.PS00255 Full length( BobuA.00616.a ) 1 485
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127330
PATRIC ID: fig|224326.49.peg.737
RefSeq: NP_212475.1
UniProt: O51316
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEYKLVIGLE IHVQLGLKTK AFCGCKNEFG GVPNSRVCPI CLGLPGSLPS VNVELINSAI LAGHATNSKI RNVVKFDRKH YYYPDLPKGY QISQNDKPIC EGGSLLIETP SGPKKINIIR IHMEEDSGKS LHLLDSENQS YVDFNRSGAP LLEIVSAPDI NSGDEAVAFL SSLREIFRYL DLSECNMENG SFRCDVNVNL IVKENGVEHK TPIAEIKNLN SFKSIKAAIE YEELRQQQEW IQFKKTLNSC GKHTRGFDDR SGVTVIQRNK ETVSDYRYFQ EPDLPLIEID DSYIDNIKNL KLIELPFHAR IRLKGQYGLS DFDVITLTAD KHLLKYFEEA VINSSDPKKV ANWILSEVLS VLNDKGISVL EFNLLPSYIT ELVEFIVAGK ISGKMAKRVF SEMMTRGVSA SVVISENQLE QVSDKFVIKQ IVLEVLDENP KSIELYKKGK DHAIKFMMGQ IMKKSSGKIN PILANEILLE SLSNV
NT Sequence
ATGGAATATA AATTAGTTAT TGGATTAGAA ATTCATGTTC AACTGGGTTT AAAAACAAAG GCTTTTTGTG GATGTAAAAA TGAGTTTGGA GGAGTTCCCA ACTCTCGTGT TTGTCCAATT TGTCTTGGAT TGCCAGGTTC ATTACCAAGT GTGAATGTAG AGCTTATTAA TAGTGCAATT TTAGCGGGGC ATGCCACAAA TTCAAAGATT AGAAATGTTG TTAAATTTGA TAGAAAGCAT TATTATTATC CAGATTTGCC AAAAGGATAT CAAATCTCGC AAAATGATAA GCCAATTTGT GAGGGGGGAA GCTTATTGAT TGAAACCCCT TCTGGACCCA AAAAGATTAA CATTATTAGA ATTCATATGG AAGAAGATTC TGGCAAGAGT CTACATTTAC TGGACAGTGA AAATCAAAGT TATGTTGATT TTAATCGCTC GGGTGCTCCT TTGCTTGAGA TTGTTTCTGC TCCAGATATT AACAGTGGAG ATGAAGCAGT TGCTTTCCTA AGCTCTTTAA GAGAAATTTT TAGGTATCTT GATTTGTCCG AATGTAATAT GGAGAATGGT TCTTTTAGAT GCGATGTAAA TGTTAATTTA ATTGTTAAAG AGAATGGTGT TGAACATAAA ACTCCTATAG CTGAAATAAA GAATTTAAAT TCTTTTAAAT CTATTAAAGC GGCCATTGAA TATGAAGAAT TAAGGCAGCA ACAGGAGTGG ATTCAATTTA AGAAAACTCT TAATAGTTGT GGTAAGCACA CTAGAGGATT TGATGATAGG AGCGGAGTAA CGGTTATTCA AAGAAATAAA GAGACAGTAT CTGATTATCG CTATTTTCAA GAACCCGACC TGCCTTTAAT AGAGATTGAT GATTCTTATA TTGATAATAT TAAAAATTTA AAGTTGATTG AACTTCCATT TCATGCAAGA ATTAGGCTTA AGGGCCAATA TGGGCTAAGT GATTTTGATG TTATTACTTT AACAGCAGAT AAGCATCTGC TTAAATATTT TGAAGAGGCT GTTATTAATT CAAGCGATCC CAAAAAAGTA GCCAATTGGA TATTGTCTGA AGTTTTAAGC GTTCTTAATG ATAAAGGAAT TAGTGTTCTT GAATTTAATT TGCTTCCAAG CTATATTACA GAGCTTGTTG AATTTATTGT TGCTGGCAAA ATAAGTGGCA AAATGGCAAA AAGGGTATTT TCAGAGATGA TGACTAGAGG AGTTTCTGCC TCTGTTGTTA TAAGTGAAAA TCAATTAGAG CAAGTAAGTG ATAAGTTTGT TATTAAGCAG ATTGTGCTTG AAGTTTTAGA TGAAAATCCT AAATCAATTG AACTTTACAA AAAGGGCAAA GACCATGCTA TCAAATTTAT GATGGGGCAA ATAATGAAAA AATCTTCAGG AAAGATTAAT CCTATACTTG CAAATGAAAT TCTTTTAGAA AGTTTATCAA ATGTA
Details for BobuA.00616.a.A1.GE26273
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/6/2009
EXPECTED MW: 56kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEYKLVIGL EIHVQLGLKT KAFCGCKNEF GGVPNSRVCP ICLGLPGSLP SVNVELINSA ILAGHATNSK IRNVVKFDRK HYYYPDLPKG YQISQNDKPI CEGGSLLIET PSGPKKINII RIHMEEDSGK SLHLLDSENQ SYVDFNRSGA PLLEIVSAPD INSGDEAVAF LSSLREIFRY LDLSXCNMEN GSFRCDVNVN LIVKENGVEH KTPIAEIKNL NSFKSIKAAI EYEELRQQQE WIQFKKTLNS CGKHTRGFDD RSGVTVIQRN KETVSDYRYF QEPDLPLIEI DDSYIDNIKN LKLIELPFHA RIRLKGQYGL SDFDVITLTA DKHLLKYFEE AVINSSDPKK VANWILSEVL SVLNDKGISV LEFNLLPSYI TELVEFIVAG KISGKMAKRV FSEMMTRGVS ASVVISENQL EQVSDKFVIK QIVLEVLDEN PKSIELYKKG KDHAIKFMMG QIMKKSSGKI NPILANEILL ESL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaat ataaattagt tattggatta gaaattcatg ttcaactggg tttaaaaaca aaggcttttt gtggatgtaa aaatgagttt ggaggagttc ccaactctcg tgtttgtcca atttgtcttg gattgccagg ttcattacca agtgtgaatg tagagcttat taatagtgca attttagcgg ggcatgccac aaattcaaag attagaaatg ttgttaaatt tgatagaaag cattattatt atccagattt gccaaaagga tatcaaatct cgcaaaatga taagccaatt tgtgaggggg gaagcttatt gattgaaacc ccttctggac ccaaaaagat taacattatt agaattcata tggaagaaga ttctggcaag agtctacatt tactggacag tgaaaatcaa agttatgttg attttaatcg ctcgggtgct cctttgcttg agattgtttc tgctccagat attaacagtg gagatgaagc agttgctttc ctaagctctt taagagaaat ttttaggtat cttgatttgt ccnaatgtaa tatggagaat ggttctttta gatgcgatgt aaatgttaat ttaattgtta aagagaatgg tgttgaacat aaaactccta tagctgaaat aaagaattta aattctttta aatctattaa agcggccatt gaatatgaag aattaaggca gcaacaggag tggattcaat ttaagaaaac tcttaatagt tgtggtaagc acactagagg atttgatgat aggagcggag taacggttat tcaaagaaat aaagagacag tatctgatta tcgctatttt caagaacccg acctgccttt aatagagatt gatgattctt atattgataa tattaaaaat ttaaagttga ttgaacttcc atttcatgca agaattaggc ttaagggcca atatgggcta agtgattttg atgttattac tttaacagca gataagcatc tgcttaaata ttttgaagag gctgttatta attcaagcga tcccaaaaaa gtagccaatt ggatattgtc tgaagtttta agcgttctta atgataaagg aattagtgtt cttgaattta atttgcttcc aagctatatt acagagcttg ttgaatttat tgttgctggc aaaataagtg gcaaaatggc aaaaagggta ttttcagaaa tgatgactag aggagtttct gcctctgttg ttataagtga aaatcaatta gagcaagtaa gtgataagtt tgttattaag cagattgtgc ttgaagtttt agatgaaaat cctaaatcaa ttgaacttta caaaaagggc aaagaccatg ctatcaaatt tatgatgggg caaataatga aaaaatcttc aggaaagatt aatcctatac ttgcaaatga aattctttta gaaagtttat ca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEYKLVIGL EIHVQLGLKT KAFCGCKNEF GGVPNSRVCP ICLGLPGSLP SVNVELINSA ILAGHATNSK IRNVVKFDRK HYYYPDLPKG YQISQNDKPI CEGGSLLIET PSGPKKINII RIHMEEDSGK SLHLLDSENQ SYVDFNRSGA PLLEIVSAPD INSGDEAVAF LSSLREIFRY LDLSECNMEN GSFRCDVNVN LIVKENGVEH KTPIAEIKNL NSFKSIKAAI EYEELRQQQE WIQFKKTLNS CGKHTRGFDD RSGVTVIQRN KETVSDYRYF QEPDLPLIEI DDSYIDNIKN LKLIELPFHA RIRLKGQYGL SDFDVITLTA DKHLLKYFEE AVINSSDPKK VANWILSEVL SVLNDKGISV LEFNLLPSYI TELVEFIVAG KISGKMAKRV FSEMMTRGVS ASVVISENQL EQVSDKFVIK QIVLEVLDEN PKSIELYKKG KDHAIKFMMG QIMKKSSGKI NPILANEILL ESLSNV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatataaa ttagttattg gattagaaat tcatgttcaa ctgggtttaa aaacaaaggc tttttgtgga tgtaaaaatg agtttggagg agttcccaac tctcgtgttt gtccaatttg tcttggattg ccaggttcat taccaagtgt gaatgtagag cttattaata gtgcaatttt agcggggcat gccacaaatt caaagattag aaatgttgtt aaatttgata gaaagcatta ttattatcca gatttgccaa aaggatatca aatctcgcaa aatgataagc caatttgtga ggggggaagc ttattgattg aaaccccttc tggacccaaa aagattaaca ttattagaat tcatatggaa gaagattctg gcaagagtct acatttactg gacagtgaaa atcaaagtta tgttgatttt aatcgctcgg gtgctccttt gcttgagatt gtttctgctc cagatattaa cagtggagat gaagcagttg ctttcctaag ctctttaaga gaaattttta ggtatcttga tttgtccgaa tgtaatatgg agaatggttc ttttagatgc gatgtaaatg ttaatttaat tgttaaagag aatggtgttg aacataaaac tcctatagct gaaataaaga atttaaattc ttttaaatct attaaagcgg ccattgaata tgaagaatta aggcagcaac aggagtggat tcaatttaag aaaactctta atagttgtgg taagcacact agaggatttg atgataggag cggagtaacg gttattcaaa gaaataaaga gacagtatct gattatcgct attttcaaga acccgacctg cctttaatag agattgatga ttcttatatt gataatatta aaaatttaaa gttgattgaa cttccatttc atgcaagaat taggcttaag ggccaatatg ggctaagtga ttttgatgtt attactttaa cagcagataa gcatctgctt aaatattttg aagaggctgt tattaattca agcgatccca aaaaagtagc caattggata ttgtctgaag ttttaagcgt tcttaatgat aaaggaatta gtgttcttga atttaatttg cttccaagct atattacaga gcttgttgaa tttattgttg ctggcaaaat aagtggcaaa atggcaaaaa gggtattttc agagatgatg actagaggag tttctgcctc tgttgttata agtgaaaatc aattagagca agtaagtgat aagtttgtta ttaagcagat tgtgcttgaa gttttagatg aaaatcctaa atcaattgaa ctttacaaaa agggcaaaga ccatgctatc aaatttatga tggggcaaat aatgaaaaaa tcttcaggaa agattaatcc tatacttgca aatgaaattc ttttagaaag tttatcaaat gtatgaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BobuA.00616.a.A1.PS00255
PURIFICATION DATe: 5/14/2009
CONCENTRATION: 11.35mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEYKLVIGL EIHVQLGLKT KAFCGCKNEF GGVPNSRVCP ICLGLPGSLP SVNVELINSA ILAGHATNSK IRNVVKFDRK HYYYPDLPKG YQISQNDKPI CEGGSLLIET PSGPKKINII RIHMEEDSGK SLHLLDSENQ SYVDFNRSGA PLLEIVSAPD INSGDEAVAF LSSLREIFRY LDLSXCNMEN GSFRCDVNVN LIVKENGVEH KTPIAEIKNL NSFKSIKAAI EYEELRQQQE WIQFKKTLNS CGKHTRGFDD RSGVTVIQRN KETVSDYRYF QEPDLPLIEI DDSYIDNIKN LKLIELPFHA RIRLKGQYGL SDFDVITLTA DKHLLKYFEE AVINSSDPKK VANWILSEVL SVLNDKGISV LEFNLLPSYI TELVEFIVAG KISGKMAKRV FSEMMTRGVS ASVVISENQL EQVSDKFVIK QIVLEVLDEN PKSIELYKKG KDHAIKFMMG QIMKKSSGKI NPILANEILL ESL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaat ataaattagt tattggatta gaaattcatg ttcaactggg tttaaaaaca aaggcttttt gtggatgtaa aaatgagttt ggaggagttc ccaactctcg tgtttgtcca atttgtcttg gattgccagg ttcattacca agtgtgaatg tagagcttat taatagtgca attttagcgg ggcatgccac aaattcaaag attagaaatg ttgttaaatt tgatagaaag cattattatt atccagattt gccaaaagga tatcaaatct cgcaaaatga taagccaatt tgtgaggggg gaagcttatt gattgaaacc ccttctggac ccaaaaagat taacattatt agaattcata tggaagaaga ttctggcaag agtctacatt tactggacag tgaaaatcaa agttatgttg attttaatcg ctcgggtgct cctttgcttg agattgtttc tgctccagat attaacagtg gagatgaagc agttgctttc ctaagctctt taagagaaat ttttaggtat cttgatttgt ccnaatgtaa tatggagaat ggttctttta gatgcgatgt aaatgttaat ttaattgtta aagagaatgg tgttgaacat aaaactccta tagctgaaat aaagaattta aattctttta aatctattaa agcggccatt gaatatgaag aattaaggca gcaacaggag tggattcaat ttaagaaaac tcttaatagt tgtggtaagc acactagagg atttgatgat aggagcggag taacggttat tcaaagaaat aaagagacag tatctgatta tcgctatttt caagaacccg acctgccttt aatagagatt gatgattctt atattgataa tattaaaaat ttaaagttga ttgaacttcc atttcatgca agaattaggc ttaagggcca atatgggcta agtgattttg atgttattac tttaacagca gataagcatc tgcttaaata ttttgaagag gctgttatta attcaagcga tcccaaaaaa gtagccaatt ggatattgtc tgaagtttta agcgttctta atgataaagg aattagtgtt cttgaattta atttgcttcc aagctatatt acagagcttg ttgaatttat tgttgctggc aaaataagtg gcaaaatggc aaaaagggta ttttcagaaa tgatgactag aggagtttct gcctctgttg ttataagtga aaatcaatta gagcaagtaa gtgataagtt tgttattaag cagattgtgc ttgaagtttt agatgaaaat cctaaatcaa ttgaacttta caaaaagggc aaagaccatg ctatcaaatt tatgatgggg caaataatga aaaaatcttc aggaaagatt aatcctatac ttgcaaatga aattctttta gaaagtttat ca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEYKLVIGL EIHVQLGLKT KAFCGCKNEF GGVPNSRVCP ICLGLPGSLP SVNVELINSA ILAGHATNSK IRNVVKFDRK HYYYPDLPKG YQISQNDKPI CEGGSLLIET PSGPKKINII RIHMEEDSGK SLHLLDSENQ SYVDFNRSGA PLLEIVSAPD INSGDEAVAF LSSLREIFRY LDLSECNMEN GSFRCDVNVN LIVKENGVEH KTPIAEIKNL NSFKSIKAAI EYEELRQQQE WIQFKKTLNS CGKHTRGFDD RSGVTVIQRN KETVSDYRYF QEPDLPLIEI DDSYIDNIKN LKLIELPFHA RIRLKGQYGL SDFDVITLTA DKHLLKYFEE AVINSSDPKK VANWILSEVL SVLNDKGISV LEFNLLPSYI TELVEFIVAG KISGKMAKRV FSEMMTRGVS ASVVISENQL EQVSDKFVIK QIVLEVLDEN PKSIELYKKG KDHAIKFMMG QIMKKSSGKI NPILANEILL ESLSNV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATATAAA TTAGTTATTG GATTAGAAAT TCATGTTCAA CTGGGTTTAA AAACAAAGGC TTTTTGTGGA TGTAAAAATG AGTTTGGAGG AGTTCCCAAC TCTCGTGTTT GTCCAATTTG TCTTGGATTG CCAGGTTCAT TACCAAGTGT GAATGTAGAG CTTATTAATA GTGCAATTTT AGCGGGGCAT GCCACAAATT CAAAGATTAG AAATGTTGTT AAATTTGATA GAAAGCATTA TTATTATCCA GATTTGCCAA AAGGATATCA AATCTCGCAA AATGATAAGC CAATTTGTGA GGGGGGAAGC TTATTGATTG AAACCCCTTC TGGACCCAAA AAGATTAACA TTATTAGAAT TCATATGGAA GAAGATTCTG GCAAGAGTCT ACATTTACTG GACAGTGAAA ATCAAAGTTA TGTTGATTTT AATCGCTCGG GTGCTCCTTT GCTTGAGATT GTTTCTGCTC CAGATATTAA CAGTGGAGAT GAAGCAGTTG CTTTCCTAAG CTCTTTAAGA GAAATTTTTA GGTATCTTGA TTTGTCCGAA TGTAATATGG AGAATGGTTC TTTTAGATGC GATGTAAATG TTAATTTAAT TGTTAAAGAG AATGGTGTTG AACATAAAAC TCCTATAGCT GAAATAAAGA ATTTAAATTC TTTTAAATCT ATTAAAGCGG CCATTGAATA TGAAGAATTA AGGCAGCAAC AGGAGTGGAT TCAATTTAAG AAAACTCTTA ATAGTTGTGG TAAGCACACT AGAGGATTTG ATGATAGGAG CGGAGTAACG GTTATTCAAA GAAATAAAGA GACAGTATCT GATTATCGCT ATTTTCAAGA ACCCGACCTG CCTTTAATAG AGATTGATGA TTCTTATATT GATAATATTA AAAATTTAAA GTTGATTGAA CTTCCATTTC ATGCAAGAAT TAGGCTTAAG GGCCAATATG GGCTAAGTGA TTTTGATGTT ATTACTTTAA CAGCAGATAA GCATCTGCTT AAATATTTTG AAGAGGCTGT TATTAATTCA AGCGATCCCA AAAAAGTAGC CAATTGGATA TTGTCTGAAG TTTTAAGCGT TCTTAATGAT AAAGGAATTA GTGTTCTTGA ATTTAATTTG CTTCCAAGCT ATATTACAGA GCTTGTTGAA TTTATTGTTG CTGGCAAAAT AAGTGGCAAA ATGGCAAAAA GGGTATTTTC AGAGATGATG ACTAGAGGAG TTTCTGCCTC TGTTGTTATA AGTGAAAATC AATTAGAGCA AGTAAGTGAT AAGTTTGTTA TTAAGCAGAT TGTGCTTGAA GTTTTAGATG AAAATCCTAA ATCAATTGAA CTTTACAAAA AGGGCAAAGA CCATGCTATC AAATTTATGA TGGGGCAAAT AATGAAAAAA TCTTCAGGAA AGATTAATCC TATACTTGCA AATGAAATTC TTTTAGAAAG TTTATCAAAT GTATGAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA