BobuA.00567.a

tRNA-guanine transglycosylase (tgt) (BB_0809)

CENTER ID: BobuA.00567.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00567.a.A1.GE26269 Full length( BobuA.00567.a ) 1 375
BobuA.00567.a.B1.GE27138 Full length( BobuA.00567.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126946
PATRIC ID: fig|224326.49.peg.1201
RefSeq: NP_212943.1
UniProt: O51749
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFSVIKNDKH FNARVGFLNL PHGRVDIPCF MPVGTLGAMK GLKHAVLEKL ECNLMLANTY HLYLRLGIKT VEKYVGLHNF TIWNKNFLTD SGGFRVFSFS DLRKIDLKGV HFKSHIDGSY HYFTSEGIFA MQEIFGSDII MPLDICSSYG IDYNEANLYT NITTNWASST FKSSKNRKEG YNGLLFLITQ GNFFKDLRKR SINDILELDS PGIAIGGISV GEPREKYLEI LEYSFLLIPK EKPRYVMGIG TPHYILNAIY YGIDIFDCFN PARITRHGSL LTDNGIMCIG RKEYKDDTSK VEKNCICTLC KRYSRGYLRH LIKSKELFGI VLASEHNIHY MFRLISKIRA AILNDDFLNF RTSYLKKYEE ENFDE
NT Sequence
ATGTTTAGTG TAATCAAGAA TGATAAGCAT TTTAATGCAA GGGTTGGATT TTTAAATCTT CCTCATGGTA GAGTAGATAT TCCTTGTTTT ATGCCAGTTG GTACTTTGGG GGCAATGAAA GGGTTAAAAC ATGCTGTTCT TGAGAAGTTA GAATGTAATT TGATGCTTGC AAATACTTAT CATTTATATT TAAGGCTGGG AATTAAAACT GTTGAAAAAT ATGTTGGTCT TCATAATTTT ACAATTTGGA ATAAAAATTT TTTAACCGAT TCGGGCGGAT TTCGGGTGTT TTCTTTTTCT GATCTGAGAA AAATTGATCT AAAAGGTGTG CATTTTAAAT CTCATATAGA TGGATCGTAC CATTATTTTA CTTCCGAGGG AATATTTGCT ATGCAAGAAA TTTTTGGCAG TGATATTATT ATGCCACTTG ACATTTGCAG TTCTTATGGG ATTGATTATA ATGAAGCCAA TTTATATACA AATATTACAA CCAATTGGGC TAGCAGCACG TTCAAATCTT CTAAAAACAG AAAAGAGGGA TACAACGGGC TTTTATTTTT AATAACTCAA GGAAATTTTT TTAAAGATTT AAGGAAAAGA AGTATCAATG ATATATTGGA ATTAGACAGC CCAGGTATTG CCATTGGAGG CATTTCTGTT GGAGAACCAA GGGAGAAATA TTTAGAAATC CTTGAATATA GTTTTTTGTT GATACCAAAA GAAAAACCAA GGTATGTAAT GGGTATTGGC ACTCCCCATT ACATACTTAA TGCCATATAT TATGGCATTG ATATTTTTGA TTGCTTTAAT CCCGCAAGAA TTACTAGGCA TGGGTCTCTT TTGACAGATA ATGGGATTAT GTGCATTGGT AGAAAGGAGT ATAAGGATGA TACTTCTAAG GTAGAGAAAA ATTGCATCTG TACTTTATGT AAAAGGTATT CAAGAGGATA TTTAAGACAT TTAATAAAAT CGAAAGAGCT TTTTGGAATA GTTTTGGCAA GTGAACATAA TATCCACTAT ATGTTTCGAT TGATTTCAAA GATCAGAGCC GCAATTCTAA ATGATGATTT TTTAAATTTT AGAACTTCAT ATTTAAAAAA GTATGAAGAG GAAAATTTCG ATGAA
Details for BobuA.00567.a.A1.GE26269
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/21/2012
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFSVIKNDK HFNARVGFLN LPHGRVDIPC FMPVGTLGAM KGLKHAVLEK LECNLMLANT YHLYLRLGIK TVEKYVGLHN FTIWNKNFLT DSGGFRVFSF SDLRKIDLKG VHFKSHIDGS YHYFTSEGIF AMQEIFGSDI IMPLDICSSY GIDYNEANLY TNITTNWASS TFKSSKNRKE GYNGLLFLIT QGNFFKDLRK RSINDILELD SPGIAIGGIS VGEPREKYLE ILEYSFLXIP KEKPRYVMGI GTPHYILNAI YYGIDIFDCF NPARITRHGS LXXDNGXMCI GRKEYKXDTS KVEKNCICTX CKRYSRGYXR HLIKSKXXFG IVXAXEHNIH XMFRXISKX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttta gtgtaatcaa gaatgataag cattttaatg caagggttgg atttttaaat cttcctcatg gtagagtaga tattccttgt tttatgccag ttggtacttt gggggcaatg aaagggttaa aacatgctgt tcttgagaag ttagaatgta atttgatgct tgcaaatact tatcatttat atttaaggct gggaattaaa actgttgaaa aatatgttgg tcttcataat tttacaattt ggaataaaaa ttttttaacc gattcgggcg gatttcgggt gttttctttt tctgatctga gaaaaattga tctaaaaggt gtgcatttta aatctcatat agatggatcg taccattatt ttacttccga gggaatattt gctatgcaag aaatttttgg cagtgatatt attatgccac ttgacatttg cagttcttat gggattgatt ataatgaagc caatttatat acaaatatta caaccaattg ggctagcagc acgttcaaat cttctaaaaa cagaaaagag ggatacaacg ggcttttatt tttaataact caaggaaatt tttttaaaga tttaaggaaa agaagtatca atgatatatt ggaattagac agcccaggta ttgccattgg aggcatttct gttggagaac caagggagaa atatttagaa atccttgaat atagtttttt gntgatacca aaagaaaaac caaggtatgt aatgggtatt ggcactcccc attacatact taatgccata tattatggca ttgatatttt tgattgcttt aatcccgcaa gaattactag gcatgggtct ctttnnanag ataatgggan tatgtgcatt ggtagaaagg agtataagna tgatacttct aaggtagaga aaaattgcat ctgtacttna tgtaaaaggt attcaagagg atatttnaga catttaataa aatcgaaaga nntttttgga atagtttngg caantgaaca taatatccac tanatgtttc gnntnatttc aaagancaga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFSVIKNDK HFNARVGFLN LPHGRVDIPC FMPVGTLGAM KGLKHAVLEK LECNLMLANT YHLYLRLGIK TVEKYVGLHN FTIWNKNFLT DSGGFRVFSF SDLRKIDLKG VHFKSHIDGS YHYFTSEGIF AMQEIFGSDI IMPLDICSSY GIDYNEANLY TNITTNWASS TFKSSKNRKE GYNGLLFLIT QGNFFKDLRK RSINDILELD SPGIAIGGIS VGEPREKYLE ILEYSFLLIP KEKPRYVMGI GTPHYILNAI YYGIDIFDCF NPARITRHGS LLTDNGIMCI GRKEYKDDTS KVEKNCICTL CKRYSRGYLR HLIKSKELFG IVLASEHNIH YMFRLISKIR AAILNDDFLN FRTSYLKKYE EENFDE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttagtgta atcaagaatg ataagcattt taatgcaagg gttggatttt taaatcttcc tcatggtaga gtagatattc cttgttttat gccagttggt actttggggg caatgaaagg gttaaaacat gctgttcttg agaagttaga atgtaatttg atgcttgcaa atacttatca tttatattta aggctgggaa ttaaaactgt tgaaaaatat gttggtcttc ataattttac aatttggaat aaaaattttt taaccgattc gggcggattt cgggtgtttt ctttttctga tctgagaaaa attgatctaa aaggtgtgca ttttaaatct catatagatg gatcgtacca ttattttact tccgagggaa tatttgctat gcaagaaatt tttggcagtg atattattat gccacttgac atttgcagtt cttatgggat tgattataat gaagccaatt tatatacaaa tattacaacc aattgggcta gcagcacgtt caaatcttct aaaaacagaa aagagggata caacgggctt ttatttttaa taactcaagg aaattttttt aaagatttaa ggaaaagaag tatcaatgat atattggaat tagacagccc aggtattgcc attggaggca tttctgttgg agaaccaagg gagaaatatt tagaaatcct tgaatatagt tttttgttga taccaaaaga aaaaccaagg tatgtaatgg gtattggcac tccccattac atacttaatg ccatatatta tggcattgat atttttgatt gctttaatcc cgcaagaatt actaggcatg ggtctctttt gacagataat gggattatgt gcattggtag aaaggagtat aaggatgata cttctaaggt agagaaaaat tgcatctgta ctttatgtaa aaggtattca agaggatatt taagacattt aataaaatcg aaagagcttt ttggaatagt tttggcaagt gaacataata tccactatat gtttcgattg atttcaaaga tcagagccgc aattctaaat gatgattttt taaattttag aacttcatat ttaaaaaagt atgaagagga aaatttcgat gaataaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BobuA.00567.a.B1.GE27138
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 88
Validated AA Sequence
MPVGTLGAMK GLKHAVLEKL ECNLMLANTY HLYLRLGIKT VEKYVGLHNF TIWDKNFLTD SGGFRVFSFS DLRKIDLKGV HFKSHIDGSY HYFTSEGIFA MQEIFGSDII MPLDICSSYG IDYNEANLYT NITTNWASST FKSSKNRKEG YNGLLFLITQ GNFFKDLRKR SINDILELDS PGIAIGGISV GEPREKYLEI LEYSFLLIPK EKPRYVMGIG TPHYILNAIY YGIDIFDCFN PARITRHGSL LTDNGIMCIG RKEYKDDTSK VEKNCICTLC KRYSRGYLRH LIKSKELFGI VLASEHNIHY MFRLISKIRA AILNDDFLNF RTSYLKKY
Validated NT Sequence
tgtaatcaag aatgataagc attttaatgc aagggttgga tttttaaatc ttcctcatgg tagagtagat attccttgtt ttatgccagt tggtactttg ggggcaatga aagggttaaa acatgctgtt cttgagaagt tagaatgtaa tttgatgctt gcaaatactt atcatttata tttaaggctg ggaattaaaa ctgttgaaaa atatgttggt cttcataatt ttacaatttg ggataaaaat tttttaaccg attcgggcgg atttcgggtg ttttcttttt ctgatctgag aaaaattgat ctaaaaggtg tgcattttaa atctcatata gatggatcgt accattattt tacttccgag ggaatatttg ctatgcaaga aatttttggc agtgatatta ttatgccact tgacatttgc agttcttatg ggattgatta taatgaagcc aatttatata caaatattac aaccaattgg gctagcagca cgttcaaatc ttctaaaaac agaaaagagg gatacaacgg gcttttattt ttaataactc aaggaaattt ttttaaagat ttaaggaaaa gaagtatcaa tgatatattg gaattagaca gcccaggtat tgccattgga ggcatttctg ttggagaacc aagggagaaa tatttagaaa tccttgaata tagttttttg ttgataccaa aagaaaaacc aaggtatgta atgggtattg gcactcccca ttacatactt aatgccatat attatggcat tgatattttt gattgcttta atcccgcaag aattactagg catgggtctc ttttgacaga taatgggatt atgtgcattg gtagaaagga gtataaggat gatacttcta aggtagagaa aaattgcatc tgtactttat gtaaaaggta ttcaagagga tatttaagac atttaataaa atcgaaagag ctttttggaa tagttttggc aagtgaacat aatatccact atatgtttcg attgatttca aagatcagag ccgcaattct aaatgatgat tttttaaatt ttagaacttc atatttaaaa aagtatgaa
Expected Protein Sequence
MAHHHHHHMF SVIKNDKHFN ARVGFLNLPH GRVDIPCFMP VGTLGAMKGL KHAVLEKLEC NLMLANTYHL YLRLGIKTVE KYVGLHNFTI WNKNFLTDSG GFRVFSFSDL RKIDLKGVHF KSHIDGSYHY FTSEGIFAMQ EIFGSDIIMP LDICSSYGID YNEANLYTNI TTNWASSTFK SSKNRKEGYN GLLFLITQGN FFKDLRKRSI NDILELDSPG IAIGGISVGE PREKYLEILE YSFLLIPKEK PRYVMGIGTP HYILNAIYYG IDIFDCFNPA RITRHGSLLT DNGIMCIGRK EYKDDTSKVE KNCICTLCKR YSRGYLRHLI KSKELFGIVL ASEHNIHYMF RLISKIRAAI LNDDFLNFRT SYLKKYEEEN FDE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta gtgtaatcaa gaatgataag cattttaatg caagggttgg atttttaaat cttcctcatg gtagagtaga tattccttgt tttatgccag ttggtacttt gggggcaatg aaagggttaa aacatgctgt tcttgagaag ttagaatgta atttgatgct tgcaaatact tatcatttat atttaaggct gggaattaaa actgttgaaa aatatgttgg tcttcataat tttacaattt ggaataaaaa ttttttaacc gattcgggcg gatttcgggt gttttctttt tctgatctga gaaaaattga tctaaaaggt gtgcatttta aatctcatat agatggatcg taccattatt ttacttccga gggaatattt gctatgcaag aaatttttgg cagtgatatt attatgccac ttgacatttg cagttcttat gggattgatt ataatgaagc caatttatat acaaatatta caaccaattg ggctagcagc acgttcaaat cttctaaaaa cagaaaagag ggatacaacg ggcttttatt tttaataact caaggaaatt tttttaaaga tttaaggaaa agaagtatca atgatatatt ggaattagac agcccaggta ttgccattgg aggcatttct gttggagaac caagggagaa atatttagaa atccttgaat atagtttttt gttgatacca aaagaaaaac caaggtatgt aatgggtatt ggcactcccc attacatact taatgccata tattatggca ttgatatttt tgattgcttt aatcccgcaa gaattactag gcatgggtct cttttgacag ataatgggat tatgtgcatt ggtagaaagg agtataagga tgatacttct aaggtagaga aaaattgcat ctgtacttta tgtaaaaggt attcaagagg atatttaaga catttaataa aatcgaaaga gctttttgga atagttttgg caagtgaaca taatatccac tatatgtttc gattgatttc aaagatcaga gccgcaattc taaatgatga ttttttaaat tttagaactt catatttaaa aaagtatgaa gaggaaaatt tcgatgaata atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat