BobuA.00566.a

tRNA (guanine-N(1)-)-methyltransferase (BB_0698)

CENTER ID: BobuA.00566.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00566.a.A1.GE26264 Full length( BobuA.00566.a ) 1 239
BobuA.00566.a.B1.GE27137 Full length( BobuA.00566.a ) 1 239
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_132581
PATRIC ID: fig|224326.49.peg.1089
RefSeq: NP_212832.1
UniProt: O51641

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFTVLSLFP AIIKPFFENS IMKKAINKGI VSFELVDVRD FSKDKHKRCD DLPYGGGAGM VLKAEPISFA LEHVESAKKT TIFLSPSGIK YSQELAYSLS KREEIVIICG RYEGIDQRII DLYVDFEISI GDYVLSSGEI AALVLIDSVY RLLDGVINPN SLLEESFGVK NGLLEYPHYT RPYDFKGIKV PEVLLSGHHA NIKNWRLVKA REKTKKNRYD LYLKYLEIIG EDNGFDKKN
NT Sequence
ATGAAATTTA CGGTTTTATC CCTTTTTCCA GCAATAATTA AGCCATTTTT TGAAAATTCA ATAATGAAAA AAGCTATTAA TAAGGGAATA GTAAGTTTTG AGCTTGTTGA TGTTAGAGAT TTTTCAAAAG ATAAGCATAA AAGATGTGAT GATTTGCCTT ATGGAGGCGG TGCTGGGATG GTATTGAAGG CCGAACCGAT TTCTTTTGCT CTTGAGCATG TAGAGTCTGC CAAGAAAACG ACAATATTTT TAAGTCCTTC TGGGATAAAG TATAGCCAAG AGTTGGCGTA TTCCTTGTCA AAAAGAGAAG AAATTGTTAT AATTTGTGGA AGATATGAAG GAATTGATCA ACGTATTATA GATTTGTATG TTGATTTTGA GATTTCTATT GGAGATTATG TTTTATCTTC AGGAGAGATT GCAGCTCTTG TTTTAATAGA TAGTGTATAT AGGTTGCTAG ATGGAGTAAT AAATCCCAAT TCTTTATTAG AAGAATCATT TGGTGTAAAA AATGGATTGC TTGAATATCC TCATTATACC AGGCCCTATG ATTTTAAGGG GATAAAGGTC CCAGAAGTTC TTCTTTCGGG ACATCATGCA AATATAAAGA ATTGGAGGCT TGTTAAAGCT AGAGAAAAAA CTAAGAAAAA TAGATATGAT TTATACCTTA AATATTTAGA GATAATAGGA GAAGATAATG GATTTGATAA GAAAAAT
Details for BobuA.00566.a.A1.GE26264
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/21/2012
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKFTVLSLF PAIIKPFFEN SIMKKAINKG IVSFELVDVR DFSKDKHKRC DDLPYGGGAG MVLKAEPISF ALEHVESAKK TTIFLSPSGI KYSQELAYSL SKREEIVIIC GRYEGIDQRI IDLYVDFEIS IGDYVLSSGE IAALVLIDSV YRLLDGVINP NSLLEESFGV KNGLLEYPHY TRPYDFKGIK VPEVLLSGHH ANIKNWRLVK AREKTKKNRY DLYLKYLEII GEDNGFDK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat ttacggtttt atcccttttt ccagcaataa ttaagccatt ttttgaaaat tcaataatga aaaaagctat taataaggga atagtaagtt ttgagcttgt tgatgttaga gatttttcaa aagataagca taaaagatgt gatgatttgc cttatggagg cggtgctggg atggtattga aggccgaacc gatttctttt gctcttgagc atgtagagtc tgccaagaaa acgacaatat ttttaagtcc ttctgggata aagtatagcc aagagttggc gtattccttg tcaaaaagag aagaaattgt tataatttgt ggaagatatg aaggaattga tcaacgtatt atagatttgt atgttgattt tgagatttct attggagatt atgttttatc ttcaggagag attgcagctc ttgttttaat agatagtgta tataggttgc tagatggagt aataaatccc aattctttat tagaagaatc atttggtgta aaaaatggat tgcttgaata tcctcattat accaggccct atgattttaa ggggataaag gtcccagaag ttcttctttc gggacatcat gcaaatataa agaattggag gcttgttaaa gctagagaaa aaactaagaa aaatagatat gatttatacc ttaaatattt agagataata ggagaagata atggatttga taaga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFTVLSLF PAIIKPFFEN SIMKKAINKG IVSFELVDVR DFSKDKHKRC DDLPYGGGAG MVLKAEPISF ALEHVESAKK TTIFLSPSGI KYSQELAYSL SKREEIVIIC GRYEGIDQRI IDLYVDFEIS IGDYVLSSGE IAALVLIDSV YRLLDGVINP NSLLEESFGV KNGLLEYPHY TRPYDFKGIK VPEVLLSGHH ANIKNWRLVK AREKTKKNRY DLYLKYLEII GEDNGFDKKN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatttacg gttttatccc tttttccagc aataattaag ccattttttg aaaattcaat aatgaaaaaa gctattaata agggaatagt aagttttgag cttgttgatg ttagagattt ttcaaaagat aagcataaaa gatgtgatga tttgccttat ggaggcggtg ctgggatggt attgaaggcc gaaccgattt cttttgctct tgagcatgta gagtctgcca agaaaacgac aatattttta agtccttctg ggataaagta tagccaagag ttggcgtatt ccttgtcaaa aagagaagaa attgttataa tttgtggaag atatgaagga attgatcaac gtattataga tttgtatgtt gattttgaga tttctattgg agattatgtt ttatcttcag gagagattgc agctcttgtt ttaatagata gtgtatatag gttgctagat ggagtaataa atcccaattc tttattagaa gaatcatttg gtgtaaaaaa tggattgctt gaatatcctc attataccag gccctatgat tttaagggga taaaggtccc agaagttctt ctttcgggac atcatgcaaa tataaagaat tggaggcttg ttaaagctag agaaaaaact aagaaaaata gatatgattt ataccttaaa tatttagaga taataggaga agataatgga tttgataaga aaaattgaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BobuA.00566.a.B1.GE27137
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK FTVLSLFPAI IKPFFENSIM KKAINKGIVS FELVDVRDFS KDKHKRCDDL PYGGGAGMVL KAEPISFALE HVESAKKTTI FLSPSGIKYS QELAYSLSKR EEIVIICGRY EGIDQRIIDL YVDFEISIGD YVLSSGEIAA LVLIDSVYRL LDGVINPNSL LEESFGVKNG LLEYPHYTRP YDFKGIKVPE VLLSGHHANI KNWRLVKARE KTKKNRYDLY LKYLEIIGED NGFDKKN
Validated NT Sequence
atcttactca cttaattttt cttatcaaat ccattatctt ctcctattat ctctaaatat ttaaggtata aatcatatct atttttctta gttttttctc tagctttaac aagcctccaa ttctttatat ttgcatgatg tcccgaaaga agaacttctg ggacctttat ccccttaaaa tcatagggcc tggtataatg aggatattca agcaatccat tttttacacc aaatgattct tctaataaag aattgggatt tattactcca tctagcaacc tatatacact atctattaaa acaagagctg caatctctcc tgaagataaa acataatctc caatagaaat ctcaaaatca acatacaaat ctataatacg ttgatcaatt ccttcatatc ttccacaaat tataacaatt tcttctcttt ttgacaagga atacgccaac tcttggctat actttatccc agaaggactt aaaaatattg tcgttttctt ggcagactct acatgctcaa gagcaaaaga aatcggttcg gccttcaata ccatcccagc accgcctcca taaggcaaat catcacatct tttatgctta tcttttgaaa aatctctaac atcaacaagc tcaaaactta ctattccctt attaatagct tttttcatta ttgaattttc aaaaaatggc ttaattattg ctggaaaaag ggataaaacc gtaaatttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMK FTVLSLFPAI IKPFFENSIM KKAINKGIVS FELVDVRDFS KDKHKRCDDL PYGGGAGMVL KAEPISFALE HVESAKKTTI FLSPSGIKYS QELAYSLSKR EEIVIICGRY EGIDQRIIDL YVDFEISIGD YVLSSGEIAA LVLIDSVYRL LDGVINPNSL LEESFGVKNG LLEYPHYTRP YDFKGIKVPE VLLSGHHANI KNWRLVKARE KTKKNRYDLY LKYLEIIGED NGFDKKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat ttacggtttt atcccttttt ccagcaataa ttaagccatt ttttgaaaat tcaataatga aaaaagctat taataaggga atagtaagtt ttgagcttgt tgatgttaga gatttttcaa aagataagca taaaagatgt gatgatttgc cttatggagg cggtgctggg atggtattga aggccgaacc gatttctttt gctcttgagc atgtagagtc tgccaagaaa acgacaatat ttttaagtcc ttctgggata aagtatagcc aagagttggc gtattccttg tcaaaaagag aagaaattgt tataatttgt ggaagatatg aaggaattga tcaacgtatt atagatttgt atgttgattt tgagatttct attggagatt atgttttatc ttcaggagag attgcagctc ttgttttaat agatagtgta tataggttgc tagatggagt aataaatccc aattctttat tagaagaatc atttggtgta aaaaatggat tgcttgaata tcctcattat accaggccct atgattttaa ggggataaag gtcccagaag ttcttctttc gggacatcat gcaaatataa agaattggag gcttgttaaa gctagagaaa aaactaagaa aaatagatat gatttatacc ttaaatattt agagataata ggagaagata atggatttga taagaaaaat tgatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat