BobuA.00564.a
glucosamine-6-phosphate deaminase (BB_0152)
| CENTER ID: | BobuA.00564.a |
| ORGANISM: | Borrelia burgdorferi B31 |
| ASSOCIATED DISEASE: | Lyme disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.00564.a.A1.GE26268 | Full length( BobuA.00564.a ) | 1 | 268 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.00564.a.A1.PW26326 | Full length( BobuA.00564.a ) | 1 | 268 | ||
| BobuA.00564.a.A1.PW26787 | Full length( BobuA.00564.a ) | 1 | 268 |
Structures
3HN6
DEPOSITED: 5/29/2009
DETERMINATION: XRay
CLONE: BobuA.00564.a.A1.GE26268
PROTEIN: BobuA.00564.a.A1.PW26326
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128189 |
| PATRIC ID: | fig|224326.49.peg.549 |
| RefSeq: | NP_212286.1 |
| UniProt: | O30564 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLIIRPTYE DISKWAANHV AQKINEFSPT KENPFILGLP TGSSPIGMYK NLIELNKNKK ISFQNVITFN MDEYIGIEEN HPESYHSFMW NNFFSHIDIK KENINILNGN ASNLKKECEE YEKKIKSFGG IMLFVGGIGP DGHIAFNEPG SSLTSRTRIK TLTQDTIIAN SRFFEGDVNK VPKNALTVGI GTIMDSQEVL IIVNGHNKAR ALKHAIEKGV NHMWTISALQ LHKNAIIVSD KNATYELKVG TVEYFNDIER KNFNNDLK
NT Sequence
ATGAGATTAA TAATCAGACC CACATACGAA GACATATCAA AATGGGCGGC TAATCATGTA GCACAAAAAA TTAATGAATT TTCCCCAACA AAAGAAAATC CATTTATCCT TGGACTTCCA ACAGGAAGCT CTCCGATTGG CATGTACAAA AACTTAATTG AATTAAATAA AAATAAAAAA ATTTCATTTC AAAATGTCAT AACTTTTAAT ATGGATGAAT ACATAGGAAT TGAAGAAAAT CATCCTGAAA GTTACCATTC ATTTATGTGG AACAATTTTT TTTCCCACAT TGACATTAAA AAAGAAAACA TAAATATACT AAACGGAAAT GCTTCAAATC TCAAAAAAGA ATGCGAAGAA TATGAAAAAA AAATCAAATC TTTCGGGGGA ATAATGCTTT TTGTAGGGGG AATAGGCCCC GATGGTCACA TTGCTTTTAA TGAGCCGGGC TCCTCCTTAA CATCAAGAAC AAGAATTAAA ACTTTAACCC AGGATACAAT TATTGCTAAT TCAAGATTTT TTGAAGGCGA TGTAAACAAA GTTCCCAAAA ATGCACTAAC TGTTGGAATT GGAACGATCA TGGATTCACA AGAAGTTTTA ATAATAGTAA ATGGACATAA CAAAGCAAGA GCATTAAAGC ATGCCATTGA AAAAGGCGTT AACCACATGT GGACAATTAG TGCACTCCAA CTACATAAAA ATGCAATCAT AGTATCTGAC AAAAATGCAA CTTACGAATT AAAAGTTGGA ACAGTAGAAT ACTTTAATGA TATAGAAAGA AAAAACTTTA ATAATGACTT AAAA
Details for BobuA.00564.a.A1.GE26268
| HARVESTED ON: | 3/2/2009 |
| SEQUENCED ON: | 3/21/2012 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLIIRPTY EDISKWAANH VAQKINEFSP TKENPFILGL PTGSSPIGMY KNLIELNKNK KISFQNVITF NMDEYIGIEE NHPESYHSFM WNNFFSHIDI KKENINILNG NASNLKKECE EYEKKIKSFG GIMLFVGGIG PDGHIAFNEP GSSLTSRTRI KTLTQDTIIA NSRFFEGDVN KVPKNALTVG IGTIMDSQEV LIIVNGHNKA RALKHAIEKG VNHMWTISAL QLHKNAIIVS DKNATYELKV GTVEYFNDIE RKNFNNDLK
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagatt aataatcaga cccacatacg aagacatatc aaaatgggcg gctaatcatg tagcacaaaa aattaatgaa ttttccccaa caaaagaaaa tccatttatc cttggacttc caacaggaag ctctccgatt ggcatgtaca aaaacttaat tgaattaaat aaaaataaaa aaatttcatt tcaaaatgtc ataactttta atatggatga atacatagga attgaagaaa atcatcctga aagttaccat tcatttatgt ggaacaattt tttttcccac attgacatta aaaaagaaaa cataaatata ctaaacggaa atgcttcaaa tctcaaaaaa gaatgcgaag aatatgaaaa aaaaatcaaa tctttcgggg gaataatgct ttttgtaggg ggaataggcc ccgatggtca cattgctttt aatgagccgg gctcctcctt aacatcaaga acaagaatta aaactttaac ccaggataca attattgcta attcaagatt ttttgaaggc gatgtaaaca aagttcccaa aaatgcacta actgttggaa ttggaacgat catggattca caagaagttt taataatagt aaatggacat aacaaagcaa gagcattaaa gcatgccatt gaaaaaggcg ttaaccacat gtggacaatt agtgcactcc aactacataa aaatgcaatc atagtatctg acaaaaatgc aacttacgaa ttaaaagttg gaacagtaga atactttaat gatatagaaa gaaaaaactt taataatgac ttaaaataat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLIIRPTY EDISKWAANH VAQKINEFSP TKENPFILGL PTGSSPIGMY KNLIELNKNK KISFQNVITF NMDEYIGIEE NHPESYHSFM WNNFFSHIDI KKENINILNG NASNLKKECE EYEKKIKSFG GIMLFVGGIG PDGHIAFNEP GSSLTSRTRI KTLTQDTIIA NSRFFEGDVN KVPKNALTVG IGTIMDSQEV LIIVNGHNKA RALKHAIEKG VNHMWTISAL QLHKNAIIVS DKNATYELKV GTVEYFNDIE RKNFNNDLK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagattaata atcagaccca catacgaaga catatcaaaa tgggcggcta atcatgtagc acaaaaaatt aatgaatttt ccccaacaaa agaaaatcca tttatccttg gacttccaac aggaagctct ccgattggca tgtacaaaaa cttaattgaa ttaaataaaa ataaaaaaat ttcatttcaa aatgtcataa cttttaatat ggatgaatac ataggaattg aagaaaatca tcctgaaagt taccattcat ttatgtggaa caattttttt tcccacattg acattaaaaa agaaaacata aatatactaa acggaaatgc ttcaaatctc aaaaaagaat gcgaagaata tgaaaaaaaa atcaaatctt tcgggggaat aatgcttttt gtagggggaa taggccccga tggtcacatt gcttttaatg agccgggctc ctccttaaca tcaagaacaa gaattaaaac tttaacccag gatacaatta ttgctaattc aagatttttt gaaggcgatg taaacaaagt tcccaaaaat gcactaactg ttggaattgg aacgatcatg gattcacaag aagttttaat aatagtaaat ggacataaca aagcaagagc attaaagcat gccattgaaa aaggcgttaa ccacatgtgg acaattagtg cactccaact acataaaaat gcaatcatag tatctgacaa aaatgcaact tacgaattaa aagttggaac agtagaatac tttaatgata tagaaagaaa aaactttaat aatgacttaa aataaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BobuA.00564.a.A1.PW26326
| PURIFICATION DATe: | 4/14/2009 |
| CONCENTRATION: | 21.85mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLIIRPTY EDISKWAANH VAQKINEFSP TKENPFILGL PTGSSPIGMY KNLIELNKNK KISFQNVITF NMDEYIGIEE NHPESYHSFM WNNFFSHIDI KKENINILNG NASNLKKECE EYEKKIKSFG GIMLFVGGIG PDGHIAFNEP GSSLTSRTRI KTLTQDTIIA NSRFFEGDVN KVPKNALTVG IGTIMDSQEV LIIVNGHNKA RALKHAIEKG VNHMWTISAL QLHKNAIIVS DKNATYELKV GTVEYFNDIE RKNFNNDLK
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagatt aataatcaga cccacatacg aagacatatc aaaatgggcg gctaatcatg tagcacaaaa aattaatgaa ttttccccaa caaaagaaaa tccatttatc cttggacttc caacaggaag ctctccgatt ggcatgtaca aaaacttaat tgaattaaat aaaaataaaa aaatttcatt tcaaaatgtc ataactttta atatggatga atacatagga attgaagaaa atcatcctga aagttaccat tcatttatgt ggaacaattt tttttcccac attgacatta aaaaagaaaa cataaatata ctaaacggaa atgcttcaaa tctcaaaaaa gaatgcgaag aatatgaaaa aaaaatcaaa tctttcgggg gaataatgct ttttgtaggg ggaataggcc ccgatggtca cattgctttt aatgagccgg gctcctcctt aacatcaaga acaagaatta aaactttaac ccaggataca attattgcta attcaagatt ttttgaaggc gatgtaaaca aagttcccaa aaatgcacta actgttggaa ttggaacgat catggattca caagaagttt taataatagt aaatggacat aacaaagcaa gagcattaaa gcatgccatt gaaaaaggcg ttaaccacat gtggacaatt agtgcactcc aactacataa aaatgcaatc atagtatctg acaaaaatgc aacttacgaa ttaaaagttg gaacagtaga atactttaat gatatagaaa gaaaaaactt taataatgac ttaaaataat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLIIRPTY EDISKWAANH VAQKINEFSP TKENPFILGL PTGSSPIGMY KNLIELNKNK KISFQNVITF NMDEYIGIEE NHPESYHSFM WNNFFSHIDI KKENINILNG NASNLKKECE EYEKKIKSFG GIMLFVGGIG PDGHIAFNEP GSSLTSRTRI KTLTQDTIIA NSRFFEGDVN KVPKNALTVG IGTIMDSQEV LIIVNGHNKA RALKHAIEKG VNHMWTISAL QLHKNAIIVS DKNATYELKV GTVEYFNDIE RKNFNNDLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGATTAATA ATCAGACCCA CATACGAAGA CATATCAAAA TGGGCGGCTA ATCATGTAGC ACAAAAAATT AATGAATTTT CCCCAACAAA AGAAAATCCA TTTATCCTTG GACTTCCAAC AGGAAGCTCT CCGATTGGCA TGTACAAAAA CTTAATTGAA TTAAATAAAA ATAAAAAAAT TTCATTTCAA AATGTCATAA CTTTTAATAT GGATGAATAC ATAGGAATTG AAGAAAATCA TCCTGAAAGT TACCATTCAT TTATGTGGAA CAATTTTTTT TCCCACATTG ACATTAAAAA AGAAAACATA AATATACTAA ACGGAAATGC TTCAAATCTC AAAAAAGAAT GCGAAGAATA TGAAAAAAAA ATCAAATCTT TCGGGGGAAT AATGCTTTTT GTAGGGGGAA TAGGCCCCGA TGGTCACATT GCTTTTAATG AGCCGGGCTC CTCCTTAACA TCAAGAACAA GAATTAAAAC TTTAACCCAG GATACAATTA TTGCTAATTC AAGATTTTTT GAAGGCGATG TAAACAAAGT TCCCAAAAAT GCACTAACTG TTGGAATTGG AACGATCATG GATTCACAAG AAGTTTTAAT AATAGTAAAT GGACATAACA AAGCAAGAGC ATTAAAGCAT GCCATTGAAA AAGGCGTTAA CCACATGTGG ACAATTAGTG CACTCCAACT ACATAAAAAT GCAATCATAG TATCTGACAA AAATGCAACT TACGAATTAA AAGTTGGAAC AGTAGAATAC TTTAATGATA TAGAAAGAAA AAACTTTAAT AATGACTTAA AATAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BobuA.00564.a.A1.PW26787
| PURIFICATION DATe: | 4/14/2009 |
| CONCENTRATION: | 27.22mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLIIRPTY EDISKWAANH VAQKINEFSP TKENPFILGL PTGSSPIGMY KNLIELNKNK KISFQNVITF NMDEYIGIEE NHPESYHSFM WNNFFSHIDI KKENINILNG NASNLKKECE EYEKKIKSFG GIMLFVGGIG PDGHIAFNEP GSSLTSRTRI KTLTQDTIIA NSRFFEGDVN KVPKNALTVG IGTIMDSQEV LIIVNGHNKA RALKHAIEKG VNHMWTISAL QLHKNAIIVS DKNATYELKV GTVEYFNDIE RKNFNNDLK
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagatt aataatcaga cccacatacg aagacatatc aaaatgggcg gctaatcatg tagcacaaaa aattaatgaa ttttccccaa caaaagaaaa tccatttatc cttggacttc caacaggaag ctctccgatt ggcatgtaca aaaacttaat tgaattaaat aaaaataaaa aaatttcatt tcaaaatgtc ataactttta atatggatga atacatagga attgaagaaa atcatcctga aagttaccat tcatttatgt ggaacaattt tttttcccac attgacatta aaaaagaaaa cataaatata ctaaacggaa atgcttcaaa tctcaaaaaa gaatgcgaag aatatgaaaa aaaaatcaaa tctttcgggg gaataatgct ttttgtaggg ggaataggcc ccgatggtca cattgctttt aatgagccgg gctcctcctt aacatcaaga acaagaatta aaactttaac ccaggataca attattgcta attcaagatt ttttgaaggc gatgtaaaca aagttcccaa aaatgcacta actgttggaa ttggaacgat catggattca caagaagttt taataatagt aaatggacat aacaaagcaa gagcattaaa gcatgccatt gaaaaaggcg ttaaccacat gtggacaatt agtgcactcc aactacataa aaatgcaatc atagtatctg acaaaaatgc aacttacgaa ttaaaagttg gaacagtaga atactttaat gatatagaaa gaaaaaactt taataatgac ttaaaataat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLIIRPTY EDISKWAANH VAQKINEFSP TKENPFILGL PTGSSPIGMY KNLIELNKNK KISFQNVITF NMDEYIGIEE NHPESYHSFM WNNFFSHIDI KKENINILNG NASNLKKECE EYEKKIKSFG GIMLFVGGIG PDGHIAFNEP GSSLTSRTRI KTLTQDTIIA NSRFFEGDVN KVPKNALTVG IGTIMDSQEV LIIVNGHNKA RALKHAIEKG VNHMWTISAL QLHKNAIIVS DKNATYELKV GTVEYFNDIE RKNFNNDLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGATTAATA ATCAGACCCA CATACGAAGA CATATCAAAA TGGGCGGCTA ATCATGTAGC ACAAAAAATT AATGAATTTT CCCCAACAAA AGAAAATCCA TTTATCCTTG GACTTCCAAC AGGAAGCTCT CCGATTGGCA TGTACAAAAA CTTAATTGAA TTAAATAAAA ATAAAAAAAT TTCATTTCAA AATGTCATAA CTTTTAATAT GGATGAATAC ATAGGAATTG AAGAAAATCA TCCTGAAAGT TACCATTCAT TTATGTGGAA CAATTTTTTT TCCCACATTG ACATTAAAAA AGAAAACATA AATATACTAA ACGGAAATGC TTCAAATCTC AAAAAAGAAT GCGAAGAATA TGAAAAAAAA ATCAAATCTT TCGGGGGAAT AATGCTTTTT GTAGGGGGAA TAGGCCCCGA TGGTCACATT GCTTTTAATG AGCCGGGCTC CTCCTTAACA TCAAGAACAA GAATTAAAAC TTTAACCCAG GATACAATTA TTGCTAATTC AAGATTTTTT GAAGGCGATG TAAACAAAGT TCCCAAAAAT GCACTAACTG TTGGAATTGG AACGATCATG GATTCACAAG AAGTTTTAAT AATAGTAAAT GGACATAACA AAGCAAGAGC ATTAAAGCAT GCCATTGAAA AAGGCGTTAA CCACATGTGG ACAATTAGTG CACTCCAACT ACATAAAAAT GCAATCATAG TATCTGACAA AAATGCAACT TACGAATTAA AAGTTGGAAC AGTAGAATAC TTTAATGATA TAGAAAGAAA AAACTTTAAT AATGACTTAA AATAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA