BobuA.00558.a

thioredoxin reductase (trxB) (BB_0515)

CENTER ID: BobuA.00558.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00558.a.A1.GE26261 Full length( BobuA.00558.a ) 1 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00558.a.A1.PS00254 Full length( BobuA.00558.a ) 1 326
BobuA.00558.a.A1.PW26690 Full length( BobuA.00558.a ) 1 326
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127532
PATRIC ID: fig|224326.49.peg.906
RefSeq: NP_212649.1
UniProt: P94284
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEFETIDIN LTKKKNLSQK EVDFIEDVII VGSGPAGLTA GIYSVMSNYK AAILEGPEPG GQLTTTTEVY NYPGFKNGIS GRNLMLNMRE QVVNLGAKTF PETVFSIKRK GNIFYLYTEN YIYKSKAVII AVGSKPKKLE TLKNSGLFWN KGISVCAICD GHLFKGKRVA VIGGGNTALS ESIYLSKLVD KVYLIVRKNN LRAIAMLRDS VAKLPNIEIL YNSEAIEVDG KSSVSSVKIF NKKDNVVYEL EVSAVFMAVG YKPNTEFLKG FLDLDEEGFI VTKDVVKTSV DGVFSCGDVS NKLYAQAITA AAEGFIASVE LGNFLK
NT Sequence
ATGTTGGAAT TTGAAACTAT TGATATAAAT CTAACCAAGA AGAAAAATCT ATCTCAAAAA GAGGTAGATT TTATTGAAGA TGTAATAATT GTAGGATCTG GCCCGGCTGG ACTAACAGCT GGGATTTATT CTGTTATGAG TAATTATAAG GCTGCTATTT TGGAAGGTCC TGAACCCGGG GGACAGCTTA CTACAACCAC AGAAGTTTAC AATTATCCTG GCTTTAAAAA TGGAATAAGT GGTAGAAATT TGATGTTAAA TATGAGGGAG CAAGTAGTAA ATCTTGGGGC TAAAACTTTT CCCGAAACCG TTTTTTCTAT AAAAAGGAAG GGTAATATTT TTTACCTTTA TACAGAAAAT TATATTTATA AAAGTAAAGC TGTTATTATT GCTGTGGGAT CAAAACCCAA AAAACTTGAA ACTCTTAAAA ATTCGGGTTT ATTTTGGAAT AAAGGTATTT CTGTTTGTGC TATTTGTGAT GGACATCTTT TTAAAGGGAA AAGGGTTGCA GTAATTGGTG GAGGCAACAC TGCCCTTTCA GAATCAATTT ATTTAAGCAA ATTGGTGGAC AAGGTTTATC TTATTGTAAG AAAAAATAAT CTTAGAGCTA TTGCTATGTT AAGAGATAGT GTTGCTAAGT TACCTAATAT TGAAATTTTG TATAATTCAG AAGCCATAGA AGTAGATGGT AAATCTTCTG TTTCTTCGGT TAAGATTTTT AATAAAAAAG ATAATGTTGT TTATGAATTA GAAGTGAGTG CTGTATTTAT GGCTGTTGGC TATAAGCCAA ATACAGAATT TTTAAAGGGA TTTTTGGATT TGGACGAAGA GGGATTTATT GTCACTAAAG ATGTTGTTAA AACAAGCGTT GATGGTGTTT TTTCATGTGG AGATGTTAGC AATAAACTTT ATGCTCAAGC CATTACTGCT GCTGCTGAGG GGTTTATTGC ATCTGTTGAG TTAGGAAATT TTTTAAAA
Details for BobuA.00558.a.A1.GE26261
HARVESTED ON: 3/2/2009
SEQUENCED ON: 3/6/2009
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMLEFETI DINLTKKKNL SQKEVDFIED VIIVGSGPAG LTAGIYSVMS NYKAAILEGP EPGGQLTTTT EVYNYPGFKN GISGRNLMLN MREQVVNLGA KTFPETVFSI KRKGNIFYLY TENYIYKSKA VIIAVGSKPK KLETLKNSGL FWNKGISVCA ICDGHLFKGK RVAVIGGGNT ALSESIYLSK LVDKVYLIVR KNNLRAIAML RDSVAKLPNI EILYNSEAIE VDGKSSVSSV KIFNKKDNVV YELEVSAVFM AVGYKPNTEF LKGFLDLDEE GFIVTKDVVK TSVDGVFSCG DVSNKLYAQA ITAAAEGFIA SVELGNFLK
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgga atttgaaact attgatataa atctaaccaa gaagaaaaat ctatctcaaa aagaggtaga ttttattgaa gatgtaataa ttgtaggatc tggcccggct ggactaacag ctgggattta ttctgttatg agtaattata aggctgctat tttggaaggt cctgaacccg ggggacagct tactacaacc acagaagttt acaattatcc tggctttaaa aatggaataa gtggtagaaa tttgatgtta aatatgaggg agcaagtagt aaatcttggg gctaaaactt ttcccgaaac cgttttttct ataaaaagga agggtaatat tttttacctt tatacagaaa attatattta taaaagtaaa gctgttatta ttgctgtggg atcaaaaccc aaaaaacttg aaactcttaa aaattcgggt ttattttgga ataaaggtat ttctgtttgt gctatttgtg atggacatct ttttaaaggg aaaagggttg cagtaattgg tggaggcaac actgcccttt cagaatcaat ttatttaagc aaattggtgg acaaggttta tcttattgta agaaaaaata atcttagagc tattgctatg ttaagagata gtgttgctaa gttacctaat attgaaattt tgtataattc agaagccata gaagtagatg gtaaatcttc tgtttcttcg gttaagattt ttaataaaaa agataatgtt gtttatgaat tagaagtgag tgctgtattt atggctgttg gctataagcc aaatacagaa tttttaaagg gatttttgga tttggacgaa gagggattta ttgtcactaa agatgttgtt aaaacaagcg ttgatggtgt tttttcatgt ggagatgtta gcaataaact ttatgctcaa gccattactg ctgctgctga ggggtttatt gcatctgttg agttaggaaa ttttttaaaa taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLEFETIDI NLTKKKNLSQ KEVDFIEDVI IVGSGPAGLT AGIYSVMSNY KAAILEGPEP GGQLTTTTEV YNYPGFKNGI SGRNLMLNMR EQVVNLGAKT FPETVFSIKR KGNIFYLYTE NYIYKSKAVI IAVGSKPKKL ETLKNSGLFW NKGISVCAIC DGHLFKGKRV AVIGGGNTAL SESIYLSKLV DKVYLIVRKN NLRAIAMLRD SVAKLPNIEI LYNSEAIEVD GKSSVSSVKI FNKKDNVVYE LEVSAVFMAV GYKPNTEFLK GFLDLDEEGF IVTKDVVKTS VDGVFSCGDV SNKLYAQAIT AAAEGFIASV ELGNFLK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggaattt gaaactattg atataaatct aaccaagaag aaaaatctat ctcaaaaaga ggtagatttt attgaagatg taataattgt aggatctggc ccggctggac taacagctgg gatttattct gttatgagta attataaggc tgctattttg gaaggtcctg aacccggggg acagcttact acaaccacag aagtttacaa ttatcctggc tttaaaaatg gaataagtgg tagaaatttg atgttaaata tgagggagca agtagtaaat cttggggcta aaacttttcc cgaaaccgtt ttttctataa aaaggaaggg taatattttt tacctttata cagaaaatta tatttataaa agtaaagctg ttattattgc tgtgggatca aaacccaaaa aacttgaaac tcttaaaaat tcgggtttat tttggaataa aggtatttct gtttgtgcta tttgtgatgg acatcttttt aaagggaaaa gggttgcagt aattggtgga ggcaacactg ccctttcaga atcaatttat ttaagcaaat tggtggacaa ggtttatctt attgtaagaa aaaataatct tagagctatt gctatgttaa gagatagtgt tgctaagtta cctaatattg aaattttgta taattcagaa gccatagaag tagatggtaa atcttctgtt tcttcggtta agatttttaa taaaaaagat aatgttgttt atgaattaga agtgagtgct gtatttatgg ctgttggcta taagccaaat acagaatttt taaagggatt tttggatttg gacgaagagg gatttattgt cactaaagat gttgttaaaa caagcgttga tggtgttttt tcatgtggag atgttagcaa taaactttat gctcaagcca ttactgctgc tgctgagggg tttattgcat ctgttgagtt aggaaatttt ttaaaataga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BobuA.00558.a.A1.PS00254
PURIFICATION DATe: 7/6/2009
CONCENTRATION: 81.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMLEFETI DINLTKKKNL SQKEVDFIED VIIVGSGPAG LTAGIYSVMS NYKAAILEGP EPGGQLTTTT EVYNYPGFKN GISGRNLMLN MREQVVNLGA KTFPETVFSI KRKGNIFYLY TENYIYKSKA VIIAVGSKPK KLETLKNSGL FWNKGISVCA ICDGHLFKGK RVAVIGGGNT ALSESIYLSK LVDKVYLIVR KNNLRAIAML RDSVAKLPNI EILYNSEAIE VDGKSSVSSV KIFNKKDNVV YELEVSAVFM AVGYKPNTEF LKGFLDLDEE GFIVTKDVVK TSVDGVFSCG DVSNKLYAQA ITAAAEGFIA SVELGNFLK
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgga atttgaaact attgatataa atctaaccaa gaagaaaaat ctatctcaaa aagaggtaga ttttattgaa gatgtaataa ttgtaggatc tggcccggct ggactaacag ctgggattta ttctgttatg agtaattata aggctgctat tttggaaggt cctgaacccg ggggacagct tactacaacc acagaagttt acaattatcc tggctttaaa aatggaataa gtggtagaaa tttgatgtta aatatgaggg agcaagtagt aaatcttggg gctaaaactt ttcccgaaac cgttttttct ataaaaagga agggtaatat tttttacctt tatacagaaa attatattta taaaagtaaa gctgttatta ttgctgtggg atcaaaaccc aaaaaacttg aaactcttaa aaattcgggt ttattttgga ataaaggtat ttctgtttgt gctatttgtg atggacatct ttttaaaggg aaaagggttg cagtaattgg tggaggcaac actgcccttt cagaatcaat ttatttaagc aaattggtgg acaaggttta tcttattgta agaaaaaata atcttagagc tattgctatg ttaagagata gtgttgctaa gttacctaat attgaaattt tgtataattc agaagccata gaagtagatg gtaaatcttc tgtttcttcg gttaagattt ttaataaaaa agataatgtt gtttatgaat tagaagtgag tgctgtattt atggctgttg gctataagcc aaatacagaa tttttaaagg gatttttgga tttggacgaa gagggattta ttgtcactaa agatgttgtt aaaacaagcg ttgatggtgt tttttcatgt ggagatgtta gcaataaact ttatgctcaa gccattactg ctgctgctga ggggtttatt gcatctgttg agttaggaaa ttttttaaaa taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLEFETIDI NLTKKKNLSQ KEVDFIEDVI IVGSGPAGLT AGIYSVMSNY KAAILEGPEP GGQLTTTTEV YNYPGFKNGI SGRNLMLNMR EQVVNLGAKT FPETVFSIKR KGNIFYLYTE NYIYKSKAVI IAVGSKPKKL ETLKNSGLFW NKGISVCAIC DGHLFKGKRV AVIGGGNTAL SESIYLSKLV DKVYLIVRKN NLRAIAMLRD SVAKLPNIEI LYNSEAIEVD GKSSVSSVKI FNKKDNVVYE LEVSAVFMAV GYKPNTEFLK GFLDLDEEGF IVTKDVVKTS VDGVFSCGDV SNKLYAQAIT AAAEGFIASV ELGNFLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGAATTT GAAACTATTG ATATAAATCT AACCAAGAAG AAAAATCTAT CTCAAAAAGA GGTAGATTTT ATTGAAGATG TAATAATTGT AGGATCTGGC CCGGCTGGAC TAACAGCTGG GATTTATTCT GTTATGAGTA ATTATAAGGC TGCTATTTTG GAAGGTCCTG AACCCGGGGG ACAGCTTACT ACAACCACAG AAGTTTACAA TTATCCTGGC TTTAAAAATG GAATAAGTGG TAGAAATTTG ATGTTAAATA TGAGGGAGCA AGTAGTAAAT CTTGGGGCTA AAACTTTTCC CGAAACCGTT TTTTCTATAA AAAGGAAGGG TAATATTTTT TACCTTTATA CAGAAAATTA TATTTATAAA AGTAAAGCTG TTATTATTGC TGTGGGATCA AAACCCAAAA AACTTGAAAC TCTTAAAAAT TCGGGTTTAT TTTGGAATAA AGGTATTTCT GTTTGTGCTA TTTGTGATGG ACATCTTTTT AAAGGGAAAA GGGTTGCAGT AATTGGTGGA GGCAACACTG CCCTTTCAGA ATCAATTTAT TTAAGCAAAT TGGTGGACAA GGTTTATCTT ATTGTAAGAA AAAATAATCT TAGAGCTATT GCTATGTTAA GAGATAGTGT TGCTAAGTTA CCTAATATTG AAATTTTGTA TAATTCAGAA GCCATAGAAG TAGATGGTAA ATCTTCTGTT TCTTCGGTTA AGATTTTTAA TAAAAAAGAT AATGTTGTTT ATGAATTAGA AGTGAGTGCT GTATTTATGG CTGTTGGCTA TAAGCCAAAT ACAGAATTTT TAAAGGGATT TTTGGATTTG GACGAAGAGG GATTTATTGT CACTAAAGAT GTTGTTAAAA CAAGCGTTGA TGGTGTTTTT TCATGTGGAG ATGTTAGCAA TAAACTTTAT GCTCAAGCCA TTACTGCTGC TGCTGAGGGG TTTATTGCAT CTGTTGAGTT AGGAAATTTT TTAAAATAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BobuA.00558.a.A1.PW26690
PURIFICATION DATe: 4/14/2009
CONCENTRATION: 28.14mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 25
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMLEFETI DINLTKKKNL SQKEVDFIED VIIVGSGPAG LTAGIYSVMS NYKAAILEGP EPGGQLTTTT EVYNYPGFKN GISGRNLMLN MREQVVNLGA KTFPETVFSI KRKGNIFYLY TENYIYKSKA VIIAVGSKPK KLETLKNSGL FWNKGISVCA ICDGHLFKGK RVAVIGGGNT ALSESIYLSK LVDKVYLIVR KNNLRAIAML RDSVAKLPNI EILYNSEAIE VDGKSSVSSV KIFNKKDNVV YELEVSAVFM AVGYKPNTEF LKGFLDLDEE GFIVTKDVVK TSVDGVFSCG DVSNKLYAQA ITAAAEGFIA SVELGNFLK
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgga atttgaaact attgatataa atctaaccaa gaagaaaaat ctatctcaaa aagaggtaga ttttattgaa gatgtaataa ttgtaggatc tggcccggct ggactaacag ctgggattta ttctgttatg agtaattata aggctgctat tttggaaggt cctgaacccg ggggacagct tactacaacc acagaagttt acaattatcc tggctttaaa aatggaataa gtggtagaaa tttgatgtta aatatgaggg agcaagtagt aaatcttggg gctaaaactt ttcccgaaac cgttttttct ataaaaagga agggtaatat tttttacctt tatacagaaa attatattta taaaagtaaa gctgttatta ttgctgtggg atcaaaaccc aaaaaacttg aaactcttaa aaattcgggt ttattttgga ataaaggtat ttctgtttgt gctatttgtg atggacatct ttttaaaggg aaaagggttg cagtaattgg tggaggcaac actgcccttt cagaatcaat ttatttaagc aaattggtgg acaaggttta tcttattgta agaaaaaata atcttagagc tattgctatg ttaagagata gtgttgctaa gttacctaat attgaaattt tgtataattc agaagccata gaagtagatg gtaaatcttc tgtttcttcg gttaagattt ttaataaaaa agataatgtt gtttatgaat tagaagtgag tgctgtattt atggctgttg gctataagcc aaatacagaa tttttaaagg gatttttgga tttggacgaa gagggattta ttgtcactaa agatgttgtt aaaacaagcg ttgatggtgt tttttcatgt ggagatgtta gcaataaact ttatgctcaa gccattactg ctgctgctga ggggtttatt gcatctgttg agttaggaaa ttttttaaaa taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLEFETIDI NLTKKKNLSQ KEVDFIEDVI IVGSGPAGLT AGIYSVMSNY KAAILEGPEP GGQLTTTTEV YNYPGFKNGI SGRNLMLNMR EQVVNLGAKT FPETVFSIKR KGNIFYLYTE NYIYKSKAVI IAVGSKPKKL ETLKNSGLFW NKGISVCAIC DGHLFKGKRV AVIGGGNTAL SESIYLSKLV DKVYLIVRKN NLRAIAMLRD SVAKLPNIEI LYNSEAIEVD GKSSVSSVKI FNKKDNVVYE LEVSAVFMAV GYKPNTEFLK GFLDLDEEGF IVTKDVVKTS VDGVFSCGDV SNKLYAQAIT AAAEGFIASV ELGNFLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGAATTT GAAACTATTG ATATAAATCT AACCAAGAAG AAAAATCTAT CTCAAAAAGA GGTAGATTTT ATTGAAGATG TAATAATTGT AGGATCTGGC CCGGCTGGAC TAACAGCTGG GATTTATTCT GTTATGAGTA ATTATAAGGC TGCTATTTTG GAAGGTCCTG AACCCGGGGG ACAGCTTACT ACAACCACAG AAGTTTACAA TTATCCTGGC TTTAAAAATG GAATAAGTGG TAGAAATTTG ATGTTAAATA TGAGGGAGCA AGTAGTAAAT CTTGGGGCTA AAACTTTTCC CGAAACCGTT TTTTCTATAA AAAGGAAGGG TAATATTTTT TACCTTTATA CAGAAAATTA TATTTATAAA AGTAAAGCTG TTATTATTGC TGTGGGATCA AAACCCAAAA AACTTGAAAC TCTTAAAAAT TCGGGTTTAT TTTGGAATAA AGGTATTTCT GTTTGTGCTA TTTGTGATGG ACATCTTTTT AAAGGGAAAA GGGTTGCAGT AATTGGTGGA GGCAACACTG CCCTTTCAGA ATCAATTTAT TTAAGCAAAT TGGTGGACAA GGTTTATCTT ATTGTAAGAA AAAATAATCT TAGAGCTATT GCTATGTTAA GAGATAGTGT TGCTAAGTTA CCTAATATTG AAATTTTGTA TAATTCAGAA GCCATAGAAG TAGATGGTAA ATCTTCTGTT TCTTCGGTTA AGATTTTTAA TAAAAAAGAT AATGTTGTTT ATGAATTAGA AGTGAGTGCT GTATTTATGG CTGTTGGCTA TAAGCCAAAT ACAGAATTTT TAAAGGGATT TTTGGATTTG GACGAAGAGG GATTTATTGT CACTAAAGAT GTTGTTAAAA CAAGCGTTGA TGGTGTTTTT TCATGTGGAG ATGTTAGCAA TAAACTTTAT GCTCAAGCCA TTACTGCTGC TGCTGAGGGG TTTATTGCAT CTGTTGAGTT AGGAAATTTT TTAAAATAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A