BobuA.00535.a

DNA-directed RNA polymerase subunit alpha (BB_0502)

CENTER ID:	BobuA.00535.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00535.a.A1.GE26267	Full length( BobuA.00535.a )		1	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00535.a.A1.PS00253	Full length( BobuA.00535.a )		1	345

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130460
PATRIC ID:	fig\|224326.49.peg.893
RefSeq:	NP_212636.1
UniProt:	O51455

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMLVEKFLKD FTIPEKIEFL KSQGDGSYGK FTIYPFERGF GITIGNTLRR VLLSSIEGYA ITAMRVQSNN KDSSSKVVSS EFDLIPGVSE DTLEIIANIK NIHLKLGEGE QRKTISFSVS GKDTNVLKAS HFERDGVEVF NKDLVIATLS HDVNLDLEFQ INYGRGYVSS EQNSKYLEEV NVIALDSIFS PIEKVSYSVE DTRVGQRSDY DKLVMEIWTT GVISAKDAIK KAASIVREFL FPLVDFEDNV NTSFEKSKSE SSNLLDMSIE KLNLSVRSLN CLAKENVRTL GELISKNAEE LSKARNFGKK SLEEIIEKLG SYRLYLGMSK EDALSVLSKN VKISE

NT Sequence

ATGATGCTTG TGGAAAAATT TTTGAAAGAT TTCACTATAC CTGAAAAAAT TGAATTTTTG AAAAGCCAAG GTGATGGGTC TTATGGTAAA TTTACGATAT ATCCTTTTGA AAGAGGCTTT GGAATTACTA TAGGTAATAC TTTAAGACGT GTGTTACTTT CTTCTATTGA AGGGTATGCG ATTACTGCTA TGAGAGTTCA GTCTAACAAT AAAGACTCTT CGTCAAAGGT TGTTTCAAGT GAATTTGATT TGATTCCTGG AGTTTCTGAA GATACTCTTG AGATTATTGC TAATATTAAA AATATTCATT TGAAACTTGG AGAAGGAGAG CAAAGAAAGA CAATAAGCTT TAGTGTTAGC GGTAAAGACA CCAATGTTTT AAAAGCTTCT CATTTTGAAA GAGATGGAGT TGAGGTTTTT AATAAAGATT TAGTTATAGC TACTTTATCA CATGATGTTA ATTTAGATCT TGAATTTCAA ATTAATTATG GTAGGGGCTA TGTGTCTTCT GAGCAAAATT CTAAGTATTT AGAAGAGGTT AATGTTATTG CTTTAGATTC TATATTTTCT CCTATAGAGA AAGTTTCGTA TTCTGTAGAA GATACTAGGG TTGGTCAAAG GTCAGATTAT GACAAGCTTG TAATGGAAAT TTGGACTACA GGTGTGATTT CTGCCAAAGA TGCAATAAAA AAGGCTGCAT CAATAGTAAG AGAGTTTTTG TTTCCTCTTG TTGATTTTGA AGACAATGTT AATACATCTT TTGAGAAATC AAAATCAGAA AGTTCTAACT TGCTTGATAT GAGTATTGAG AAATTGAATT TGTCAGTCAG ATCTTTAAAT TGTTTGGCCA AAGAAAATGT TAGGACTTTA GGAGAACTTA TTAGTAAAAA CGCAGAAGAG CTTTCTAAAG CTAGAAATTT TGGGAAAAAA AGTTTGGAAG AGATAATCGA AAAACTTGGT TCTTATCGAT TATATTTAGG AATGTCTAAA GAAGATGCTC TATCTGTATT GAGTAAGAAT GTTAAAATAT CTGAA

Details for BobuA.00535.a.A1.GE26267

HARVESTED ON:	3/2/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	40kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMMLVEKFLK DFTIPEKIEF LKSQGDGSYG KFTIYPFERG FGITIGNTLR RVLLSSIEGY AITAMRVQSN NKDSSSKVVS SEFDLIPGVS EDTLEIIANI KNIHLKLGEG EQRKTISFSV SGKDTNVLKA SHFERDGVEV FNKDLVIATL SHDVNLDLEF QINYGRGYVS SEQNSKYLEE VNVIALDSIF SPIEKVSYSV EDTRVGQRSD YDKLVMEIWT TGVISAKDAI KKAASIVREF LFPLVDFEDN VNTSFEKSKS ESSNLLDMSI EKLNLSVRSL NCLAKENVRT LGELISKNAE ELSKARNFGK KSLEEIIEKL GSYRLYLGMS KEDALSVLSK NVKISE

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatgc ttgtggaaaa atttttgaaa gatttcacta tacctgaaaa aattgaattt ttgaaaagcc aaggtgatgg gtcttatggt aaatttacga tatatccttt tgaaagaggc tttggaatta ctataggtaa tactttaaga cgtgtgttac tttcttctat tgaagggtat gcgattactg ctatgagagt tcagtctaac aataaagact cttcgtcaaa ggttgtttca agtgaatttg atttgattcc tggagtttct gaagatactc ttgagattat tgctaatatt aaaaatattc atttgaaact tggagaagga gagcaaagaa agacaataag ctttagtgtt agcggtaaag acaccaatgt tttaaaagct tctcattttg aaagagatgg agttgaggtt tttaataaag atttagttat agctacttta tcacatgatg ttaatttaga tcttgaattt caaattaatt atggtagggg ctatgtgtct tctgagcaaa attctaagta tttagaagag gttaatgtta ttgctttaga ttctatattt tctcctatag agaaagtttc gtattctgta gaagatacta gggttggtca aaggtcagat tatgacaagc ttgtaatgga aatttggact acaggtgtga tttctgccaa agatgcaata aaaaaggctg catcaatagt aagagagttt ttgtttcctc ttgttgattt tgaagacaat gttaatacat cttttgagaa atcaaaatca gaaagttcta acttgcttga tatgagtatt gagaaattga atttgtcagt cagatcttta aattgtttgg ccaaagaaaa tgttaggact ttaggagaac ttattagtaa aaacgcagaa gagctttcta aagctagaaa ttttgggaaa aaaagtttgg aagagataat cgaaaaactt ggttcttatc gattatattt aggaatgtct aaagaagatg ctctatctgt attgagtaag aatgttaaaa tatctgaata ataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgcttgtg gaaaaatttt tgaaagattt cactatacct gaaaaaattg aatttttgaa aagccaaggt gatgggtctt atggtaaatt tacgatatat ccttttgaaa gaggctttgg aattactata ggtaatactt taagacgtgt gttactttct tctattgaag ggtatgcgat tactgctatg agagttcagt ctaacaataa agactcttcg tcaaaggttg tttcaagtga atttgatttg attcctggag tttctgaaga tactcttgag attattgcta atattaaaaa tattcatttg aaacttggag aaggagagca aagaaagaca ataagcttta gtgttagcgg taaagacacc aatgttttaa aagcttctca ttttgaaaga gatggagttg aggtttttaa taaagattta gttatagcta ctttatcaca tgatgttaat ttagatcttg aatttcaaat taattatggt aggggctatg tgtcttctga gcaaaattct aagtatttag aagaggttaa tgttattgct ttagattcta tattttctcc tatagagaaa gtttcgtatt ctgtagaaga tactagggtt ggtcaaaggt cagattatga caagcttgta atggaaattt ggactacagg tgtgatttct gccaaagatg caataaaaaa ggctgcatca atagtaagag agtttttgtt tcctcttgtt gattttgaag acaatgttaa tacatctttt gagaaatcaa aatcagaaag ttctaacttg cttgatatga gtattgagaa attgaatttg tcagtcagat ctttaaattg tttggccaaa gaaaatgtta ggactttagg agaacttatt agtaaaaacg cagaagagct ttctaaagct agaaattttg ggaaaaaaag tttggaagag ataatcgaaa aacttggttc ttatcgatta tatttaggaa tgtctaaaga agatgctcta tctgtattga gtaagaatgt taaaatatct gaataaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BobuA.00535.a.A1.PS00253

PURIFICATION DATe:	4/21/2009
CONCENTRATION:	16.34mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGCTTGTG GAAAAATTTT TGAAAGATTT CACTATACCT GAAAAAATTG AATTTTTGAA AAGCCAAGGT GATGGGTCTT ATGGTAAATT TACGATATAT CCTTTTGAAA GAGGCTTTGG AATTACTATA GGTAATACTT TAAGACGTGT GTTACTTTCT TCTATTGAAG GGTATGCGAT TACTGCTATG AGAGTTCAGT CTAACAATAA AGACTCTTCG TCAAAGGTTG TTTCAAGTGA ATTTGATTTG ATTCCTGGAG TTTCTGAAGA TACTCTTGAG ATTATTGCTA ATATTAAAAA TATTCATTTG AAACTTGGAG AAGGAGAGCA AAGAAAGACA ATAAGCTTTA GTGTTAGCGG TAAAGACACC AATGTTTTAA AAGCTTCTCA TTTTGAAAGA GATGGAGTTG AGGTTTTTAA TAAAGATTTA GTTATAGCTA CTTTATCACA TGATGTTAAT TTAGATCTTG AATTTCAAAT TAATTATGGT AGGGGCTATG TGTCTTCTGA GCAAAATTCT AAGTATTTAG AAGAGGTTAA TGTTATTGCT TTAGATTCTA TATTTTCTCC TATAGAGAAA GTTTCGTATT CTGTAGAAGA TACTAGGGTT GGTCAAAGGT CAGATTATGA CAAGCTTGTA ATGGAAATTT GGACTACAGG TGTGATTTCT GCCAAAGATG CAATAAAAAA GGCTGCATCA ATAGTAAGAG AGTTTTTGTT TCCTCTTGTT GATTTTGAAG ACAATGTTAA TACATCTTTT GAGAAATCAA AATCAGAAAG TTCTAACTTG CTTGATATGA GTATTGAGAA ATTGAATTTG TCAGTCAGAT CTTTAAATTG TTTGGCCAAA GAAAATGTTA GGACTTTAGG AGAACTTATT AGTAAAAACG CAGAAGAGCT TTCTAAAGCT AGAAATTTTG GGAAAAAAAG TTTGGAAGAG ATAATCGAAA AACTTGGTTC TTATCGATTA TATTTAGGAA TGTCTAAAGA AGATGCTCTA TCTGTATTGA GTAAGAATGT TAAAATATCT GAATAAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA