BobuA.00529.a

response regulatory protein (rrp-2) (BB_0763)

CENTER ID:	BobuA.00529.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00529.a.A1.GE26265	Full length( BobuA.00529.a )		1	453

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|224326.49.peg.1154
RefSeq:	NP_212897.1
UniProt:	O51704

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKMSKILVAD DEKNIREGIA TYLEDEGYFV FTASDGEEAL ETIENENLDV IISDLRMPQI SGEKLLKIVK EKNLGIPFII LTAHGTVDSA VDAMREGAYD FLTKPLDLER LLLIIKRSLN KKENNDNENA NLENILIRKD LKYYEKIMGK SLLMQKIFEL VIKIAKSNAS ILITGESGVG KEIIADAIFD LSNRNDKPFI KVNCAALSES ILESELFGHE KGAFTGAISK KKGRFELANK GTIFLDEIAE ISPEIQVKLL RVLQNKTFER VGGEATIKVD IRLLAATNKN IEEEIKKEKF REDLFYRLNI ININIPPLRE RKDDISYLTN ILIKDVAKEN NREEKTLSND AMKALYYYDW PGNIRELKNV LESALILSKG KQITKEDLPA KIKNNENLIF KITLPXGISL KEAEKEIIKQ TLFHSKNNKS KCAEILKIGR KTLHNKIIEY NID

NT Sequence

TTGAAAATGA GCAAAATACT TGTAGCTGAT GATGAAAAGA ATATTAGAGA AGGAATTGCT ACTTATCTTG AGGATGAAGG ATATTTTGTT TTCACTGCTA GTGACGGAGA AGAAGCTCTT GAAACAATTG AAAATGAAAA TCTTGATGTA ATAATATCTG ACCTGAGAAT GCCCCAGATA TCTGGAGAAA AATTGCTCAA AATAGTTAAA GAAAAAAACT TGGGAATACC TTTTATTATT CTAACAGCCC ACGGAACAGT TGATTCTGCT GTAGATGCCA TGAGAGAGGG TGCTTATGAT TTTTTAACAA AGCCCTTAGA CCTTGAAAGA CTTTTGCTAA TAATAAAAAG ATCACTAAAT AAAAAAGAAA ATAACGATAA TGAAAATGCT AATTTAGAAA ATATACTAAT AAGAAAAGAT CTAAAATACT ATGAAAAAAT CATGGGAAAA TCCCTATTAA TGCAAAAAAT TTTTGAACTT GTAATAAAAA TAGCAAAATC AAATGCATCT ATTCTTATAA CGGGCGAAAG CGGTGTTGGT AAAGAAATAA TAGCAGATGC TATTTTTGAT CTTTCAAATA GAAATGACAA ACCATTTATA AAAGTAAATT GCGCAGCACT TTCTGAAAGC ATTCTTGAAA GTGAACTTTT TGGCCATGAA AAAGGAGCAT TCACTGGAGC AATTTCCAAA AAAAAAGGCA GATTTGAACT TGCAAACAAA GGCACAATTT TTCTTGATGA GATAGCAGAA ATTTCACCTG AAATTCAAGT CAAGCTTTTA AGAGTACTGC AAAACAAAAC TTTTGAACGT GTTGGGGGAG AAGCTACAAT TAAAGTTGAT ATCAGGCTTC TGGCTGCAAC AAACAAAAAC ATTGAAGAGG AAATTAAAAA GGAAAAATTT AGAGAAGATT TATTTTATAG ATTAAATATC ATTAATATAA ACATACCGCC TTTAAGAGAA AGAAAAGATG ATATATCTTA TTTAACAAAC ATACTAATAA AAGACGTCGC AAAGGAAAAC AATAGAGAAG AAAAAACTCT TTCTAATGAT GCAATGAAAG CTCTCTATTA TTACGATTGG CCAGGAAATA TTAGAGAATT AAAAAATGTG CTTGAAAGTG CATTAATATT ATCAAAAGGC AAACAAATCA CTAAAGAAGA TTTGCCAGCA AAAATCAAAA ATAATGAAAA TCTTATATTT AAAATAACAC TACCAATNGG AATTAGCCTA AAAGAAGCTG AAAAAGAAAT AATAAAACAA ACACTTTTTC ATTCCAAAAA CAACAAAAGC AAATGCGCCG AAATACTAAA AATAGGAAGA AAAACTTTAC ACAATAAAAT AATCGAATAT AATATTGAT

Details for BobuA.00529.a.A1.GE26265

HARVESTED ON:	3/2/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	53kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKMSKILV ADDEKNIREG IATYLEDEGY FVFTASDGEE ALETIENENL DVIISDLRMP QISGEKLLKI VKEKNLGIPF IILTAHGTVD SAVDAMREGA YDFLTKPLDL ERLLLIIKRS LNKKENNDNE NANLENILIR KDLKYYEKIM GKSLLMQKIF ELVIKIAKSN ASILITGESG VGKEIIADAI FDLSNRNDKP FIKVNCAALS ESILESELFG HEKGAFTGAI SKKKGRFELA NKGTIFLDEI AEISPEIQVK LLRVLQNKTF ERVGGEATIK VDIRLLAATN KNIEEEIKKE KFREDLFYRL NIININIPPL RERKDDISYL TNILIKDVAK ENNREEKTLS NDAMKALYYY DWPGNIRELK NVLESALILS KGKQITKEDL PAKIKNNENL IFKITLPIGI SLKEAEKEII KQTLFHSKNN KSKCAEILKI GRKTLHNKII EYNID

Validated NT Sequence

tttangaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttgaa aatgagcaaa atacttgtag ctgatgatga aaagaatatt agagaaggaa ttgctactta tcttgaggat gaaggatatt ttgttttcac tgctagtgac ggagaagaag ctcttgaaac aattgaaaat gaaaatcttg atgtaataat atctgacctg agaatgcccc agatatctgg agaaaaattg ctcaaaatag ttaaagaaaa aaacttggga atacctttta ttattctaac agcccacgga acagttgatt ctgctgtaga tgccatgaga gagggtgctt atgatttttt aacaaagccc ttagaccttg aaagactttt gctaataata aaaagatcac taaataaaaa agaaaataac gataatgaaa atgctaattt agaaaatata ctaataagaa aagatctaaa atactatgaa aaaatcatgg gaaaatccct attaatgcaa aaaatttttg aacttgtaat aaaaatagca aaatcaaatg catctattct tataacgggc gaaagcggtg ttggtaaaga aataatagca gatgctattt ttgatctttc aaatagaaat gacaaaccat ttataaaagt aaattgcgca gcactttctg aaagcattct tgaaagtgaa ctttttggcc atgaaaaggg agcattcact ggagcaattt ccaaaaaaaa aggcagattt gaacttgcaa acaaaggcac aatttttctt gatgagatag cagaaatttc acctgaaatt caagtcaagc ttttaagagt actgcaaaac aaaacttttg aacgtgttgg gggagaagct acaattaaag ttgatatcag gcttctggct gcaacaaaca aaaacattga agaggaaatt aaaaaggaaa aatttagaga agatttattt tatagattaa atatcattaa tataaacata ccgcctttaa gagaaagaaa agatgatata tcttatttaa caaacatact aataaaagac gtcgcaaagg aaaacaatag agaagaaaaa actctttcta atgatgcaat gaaagctctc tattattacg attggccagg aaatattaga gaattaaaaa atgtgcttga aagtgcatta atattatcaa aaggcaaaca aatcactaaa gaagatttgc cagcaaaaat caaaaataat gaaaatctta tatttaaaat aacactacca ataggaatta gcctaaaaga agctgaaaaa gaaataataa aacaaacact ttttcattcc aaaaacaaca aaagcaaatg cgccgaaata ctaaaaatag gaagaaaaac tttacacaat aaaataatcg aatataatat tgattaataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLKMSKILV ADDEKNIREG IATYLEDEGY FVFTASDGEE ALETIENENL DVIISDLRMP QISGEKLLKI VKEKNLGIPF IILTAHGTVD SAVDAMREGA YDFLTKPLDL ERLLLIIKRS LNKKENNDNE NANLENILIR KDLKYYEKIM GKSLLMQKIF ELVIKIAKSN ASILITGESG VGKEIIADAI FDLSNRNDKP FIKVNCAALS ESILESELFG HEKGAFTGAI SKKKGRFELA NKGTIFLDEI AEISPEIQVK LLRVLQNKTF ERVGGEATIK VDIRLLAATN KNIEEEIKKE KFREDLFYRL NIININIPPL RERKDDISYL TNILIKDVAK ENNREEKTLS NDAMKALYYY DWPGNIRELK NVLESALILS KGKQITKEDL PAKIKNNENL IFKITLPXGI SLKEAEKEII KQTLFHSKNN KSKCAEILKI GRKTLHNKII EYNID

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaaaatg agcaaaatac ttgtagctga tgatgaaaag aatattagag aaggaattgc tacttatctt gaggatgaag gatattttgt tttcactgct agtgacggag aagaagctct tgaaacaatt gaaaatgaaa atcttgatgt aataatatct gacctgagaa tgccccagat atctggagaa aaattgctca aaatagttaa agaaaaaaac ttgggaatac cttttattat tctaacagcc cacggaacag ttgattctgc tgtagatgcc atgagagagg gtgcttatga ttttttaaca aagcccttag accttgaaag acttttgcta ataataaaaa gatcactaaa taaaaaagaa aataacgata atgaaaatgc taatttagaa aatatactaa taagaaaaga tctaaaatac tatgaaaaaa tcatgggaaa atccctatta atgcaaaaaa tttttgaact tgtaataaaa atagcaaaat caaatgcatc tattcttata acgggcgaaa gcggtgttgg taaagaaata atagcagatg ctatttttga tctttcaaat agaaatgaca aaccatttat aaaagtaaat tgcgcagcac tttctgaaag cattcttgaa agtgaacttt ttggccatga aaaaggagca ttcactggag caatttccaa aaaaaaaggc agatttgaac ttgcaaacaa aggcacaatt tttcttgatg agatagcaga aatttcacct gaaattcaag tcaagctttt aagagtactg caaaacaaaa cttttgaacg tgttggggga gaagctacaa ttaaagttga tatcaggctt ctggctgcaa caaacaaaaa cattgaagag gaaattaaaa aggaaaaatt tagagaagat ttattttata gattaaatat cattaatata aacataccgc ctttaagaga aagaaaagat gatatatctt atttaacaaa catactaata aaagacgtcg caaaggaaaa caatagagaa gaaaaaactc tttctaatga tgcaatgaaa gctctctatt attacgattg gccaggaaat attagagaat taaaaaatgt gcttgaaagt gcattaatat tatcaaaagg caaacaaatc actaaagaag atttgccagc aaaaatcaaa aataatgaaa atcttatatt taaaataaca ctaccaatng gaattagcct aaaagaagct gaaaaagaaa taataaaaca aacacttttt cattccaaaa acaacaaaag caaatgcgcc gaaatactaa aaataggaag aaaaacttta cacaataaaa taatcgaata taatattgat taaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa