BobuA.00515.a

S-adenosylmethionine synthetase (BB_0376)

CENTER ID:	BobuA.00515.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00515.a.B1.GE27136	Full length( BobuA.00515.a )		1	392

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126734
PATRIC ID:	fig\|224326.49.peg.771
RefSeq:	NP_212510.1
UniProt:	O50163

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKIIAANQT LTSEAVSEGH PDKIADQISD AILDEILKED KNAKVACEVI IAQNLVVIAG EINSPVKKNI DIKEVAKNII KDIGYTNIDY GLDYKTITVI DAIGNQSRDI INAIEKKGSN ALGAGDQGII FGYACDETKN FLPAPYELAN SILKKASNLR KSGAIKWLRP DSKSQVTIEY DKNRNPVKIK NIIVSHQHHP NISQKLIQQT IIEEIIKPTV QDKSMLDENT AYCINPSGNF VIGGPTGDTG LTGRKIIADS YGGFARHGGG AYSGKDATKV DRSAAYMARY IAKNMVAAGI SKEFELQLAY AIGIENPISI QITGGINDPK YANKILNFIV NNFDLTPNGI IEKLKLKQPI YLKTCTYGHF GKNEFEWEKL DFVKKIQTAL KK

NT Sequence

ATGAATAAAA TAATAGCAGC GAACCAAACT TTAACTTCTG AGGCTGTATC TGAAGGACAT CCAGACAAAA TTGCAGATCA AATCTCTGAT GCCATCCTTG ATGAAATACT AAAAGAAGAT AAAAATGCAA AAGTAGCTTG CGAGGTCATA ATTGCACAAA ATTTAGTAGT AATAGCAGGA GAAATAAATA GTCCTGTAAA AAAAAACATA GATATAAAAG AGGTTGCTAA GAATATCATC AAAGATATAG GCTATACAAA TATTGATTAT GGGCTTGATT ACAAAACAAT AACGGTAATA GACGCTATTG GCAATCAATC ACGTGACATT ATAAACGCAA TTGAAAAAAA GGGATCCAAT GCCCTTGGAG CAGGGGATCA GGGAATAATA TTTGGATATG CCTGCGATGA AACAAAAAAT TTTTTACCTG CTCCTTATGA ACTAGCCAAT TCAATTCTAA AAAAAGCTAG CAATCTTAGA AAATCAGGAG CAATAAAATG GTTAAGGCCT GACTCAAAAT CTCAAGTTAC CATAGAATAC GATAAAAACA GAAACCCTGT AAAAATAAAA AATATTATAG TCTCTCATCA ACACCATCCA AACATCTCCC AAAAACTAAT ACAACAAACA ATAATTGAAG AAATCATCAA GCCCACTGTT CAAGACAAGT CAATGCTTGA TGAAAATACT GCTTATTGCA TTAATCCTTC TGGAAATTTT GTAATTGGAG GCCCTACCGG AGACACTGGG CTTACAGGAA GAAAAATTAT TGCAGATAGC TATGGAGGAT TTGCAAGACA CGGAGGGGGA GCATACAGCG GAAAAGATGC CACAAAAGTA GATAGATCAG CTGCCTACAT GGCAAGATAT ATTGCAAAAA ATATGGTGGC AGCCGGAATT TCTAAAGAAT TTGAACTACA GCTCGCATAT GCAATTGGAA TTGAAAACCC AATATCTATT CAAATAACTG GGGGAATAAA TGATCCTAAA TATGCAAACA AAATATTAAA TTTTATTGTT AATAACTTCG ATTTAACTCC CAACGGTATA ATTGAAAAAT TAAAACTAAA ACAACCCATA TATCTTAAAA CTTGCACTTA TGGTCATTTT GGAAAAAATG AATTTGAATG GGAAAAATTA GATTTTGTAA AAAAAATACA AACAGCGCTA AAAAAA

Details for BobuA.00515.a.B1.GE27136

HARVESTED ON:	8/17/2009
SEQUENCED ON:	10/20/2009
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN KIIAANQTLT SEAVSEGHPD KIADQISDAI LDEILKEDKN AKVACEVIIA QNLVVIAGEI NSPVKKNIDI KEVAKNIIKD IGYTNIDYGL DYKTITVIDA IGNQSRDIIN AIEKKGSNAL GAGDQGIIFG YACDETKNFL PAPYELANSI LKKASNLRKS GAIKWLRPDS KSQVTIEYDK NRNPVKIKNI IVSHQHHPNI SQKLIQQTII EEIIKPTVQD KSMLDENTAY CINPSGNFVI GGPTGDTGLT GRKIIADSYG GFARHGGGAY SGKDATKVDR SAAYMARYIA KNMVAAGISK EFELQLAYAI GIENPISIQI TGGINDPKYA NKILNFIVNN FDLTPNGIIE KLKLKQPIYL KTCTYGHFGK NEFEWEKLDF VKKIQTALKK

Validated NT Sequence

tacaaaatct aatttttccc attcaaattc attttttcca aaatgaccat aagtgcaagt tttaagatat atgggttgtt ttagttttaa tttttcaatt ataccgttgg gagttaaatc gaagttatta acaataaaat ttaatatttt gtttgcatat ttaggatcat ttattccccc agttatttga atagatattg ggttttcaat tccaattgca tatgcgagct gtagttcaaa ttctttagaa attccggctg ccaccatatt ttttgcaata tatcttgcca tgtaggcagc tgatctatct acttttgtgg catcttttcc gctgtatgct ccccctccgt gtcttgcaaa tcctccatag ctatctgcaa taatttttct tcctgtaagc ccagtgtctc cggtagggcc tccaattaca aaatttccag aaggattaat gcaataagca gtattttcat caagcattga cttgtcttga acagtgggct tgatgatttc ttcaattatt gtttgttgta ttagtttttg ggagatgttt ggatggtgtt gatgagagac tataatattt tttattttta cagggtttct gtttttatcg tattctatgg taacttgaga ttttgagtca ggccttaacc attttattgc tcctgatttt ctaagattgc tagctttttt tagaattgaa ttggctagtt cataaggagc aggtaaaaaa ttttttgttt catcgcaggc atatccaaat attattccct gatcccctgc tccaagggca ttggatccct ttttttcaat tgcgtttata atgtcacgtg attgattgcc aatagcgtct attaccgtta ttgttttgta atcaagccca taatcaatat ttgtatagcc tatatctttg atgatattct tagcaacctc ttttatatct atgttttttt ttacaggact atttatttct cctgctatta ctactaaatt ttgtgcaatt atgacctcgc aagctacttt tgcattttta tcttctttta gtatttcatc aaggatggca tcagagattt gatctgcaat tttgtctgga tgtccttcag atacagcctc agaagttaaa gtttggttcg ctgct

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata aaataatagc agcgaaccaa actttaactt ctgaggctgt atctgaagga catccagaca aaattgcaga tcaaatctct gatgccatcc ttgatgaaat actaaaagaa gataaaaatg caaaagtagc ttgcgaggtc ataattgcac aaaatttagt agtaatagca ggagaaataa atagtcctgt aaaaaaaaac atagatataa aagaggttgc taagaatatc atcaaagata taggctatac aaatattgat tatgggcttg attacaaaac aataacggta atagacgcta ttggcaatca atcacgtgac attataaacg caattgaaaa aaagggatcc aatgcccttg gagcagggga tcagggaata atatttggat atgcctgcga tgaaacaaaa aattttttac ctgctcctta tgaactagcc aattcaattc taaaaaaagc tagcaatctt agaaaatcag gagcaataaa atggttaagg cctgactcaa aatctcaagt taccatagaa tacgataaaa acagaaaccc tgtaaaaata aaaaatatta tagtctctca tcaacaccat ccaaacatct cccaaaaact aatacaacaa acaataattg aagaaatcat caagcccact gttcaagaca agtcaatgct tgatgaaaat actgcttatt gcattaatcc ttctggaaat tttgtaattg gaggccctac cggagacact gggcttacag gaagaaaaat tattgcagat agctatggag gatttgcaag acacggaggg ggagcataca gcggaaaaga tgccacaaaa gtagatagat cagctgccta catggcaaga tatattgcaa aaaatatggt ggcagccgga atttctaaag aatttgaact acagctcgca tatgcaattg gaattgaaaa cccaatatct attcaaataa ctgggggaat aaatgatcct aaatatgcaa acaaaatatt aaattttatt gttaataact tcgatttaac tcccaacggt ataattgaaa aattaaaact aaaacaaccc atatatctta aaacttgcac ttatggtcat tttggaaaaa atgaatttga atgggaaaaa ttagattttg taaaaaaaat acaaacagcg ctaaaaaaat gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat