BobuA.00512.a

50S ribosomal protein L4 (BB_0479)

CENTER ID:	BobuA.00512.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00512.a.A1.GE26262	Full length( BobuA.00512.a )		1	209

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127316
PATRIC ID:	fig\|224326.49.peg.870
RefSeq:	NP_212613.1
UniProt:	P94268

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MERKVFSKDG KEIGTINLDD RVFNIEISHG SIYNAIKNEL SNLRVGTSST KTRSEVRGSS KKPWKQKGTG RARVGTKRNP VWIGGGIALG PKPRDYSYRL PKKVKKLAFK SVLSLRAADE NSFKVIENFN VESGKTKDLA LIIKNFASFN GKVVILLGND DQMIKRAGKN IRDLKILSFD KLRVVDLFYA KNLIALESAV NKLNEFYIK

NT Sequence

ATGGAAAGAA AAGTTTTTTC TAAAGATGGG AAAGAGATTG GAACTATAAA TTTGGATGAT AGAGTTTTTA ATATAGAAAT TAGTCATGGG TCTATTTATA ATGCTATAAA AAACGAGTTG TCTAATCTTA GGGTTGGAAC ATCTTCAACT AAAACCAGAT CAGAGGTTAG GGGTAGTTCT AAAAAGCCTT GGAAGCAAAA AGGAACCGGT AGAGCTAGGG TTGGTACAAA GCGAAATCCA GTTTGGATTG GCGGAGGTAT AGCATTAGGG CCAAAGCCTA GGGATTATAG CTATAGATTG CCTAAAAAGG TAAAAAAGCT TGCATTTAAG TCTGTATTAA GTTTGCGTGC TGCTGATGAA AATAGTTTTA AAGTTATTGA GAATTTTAAT GTTGAATCTG GAAAAACGAA AGATCTTGCT TTAATAATAA AAAATTTTGC AAGTTTTAAT GGTAAGGTGG TTATTCTTTT GGGCAATGAT GATCAGATGA TAAAAAGGGC TGGTAAAAAT ATAAGAGATT TAAAGATTTT ATCTTTTGAT AAACTTAGGG TTGTTGATTT ATTTTATGCT AAGAATTTAA TAGCTCTAGA ATCCGCTGTT AACAAGCTCA ATGAGTTTTA CATTAAA

Details for BobuA.00512.a.A1.GE26262

HARVESTED ON:	3/2/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMERKVFSKD GKEIGTINLD DRVFNIEISH GSIYNAIKNE LSNLRVGTSS TKTRSEVRGS SKKPWKQKGT GRARVGTKRN PVWIGGGIAL GPKPRDYSYR LPKKVKKLAF KSVLSLRAAD ENSFKVIENF NVESGKTKDL ALIIKNFASF NGKVVILLGN DDQMIKRAGK NIRDLKILSF DKLRVVDLFY AKNLIALESA VNKLNEFYIK

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaaa gaaaagtttt ttctaaagat gggaaagaga ttggaactat aaatttggat gatagagttt ttaatataga aattagtcat gggtctattt ataatgctat aaaaaacgag ttgtctaatc ttagggttgg aacatcttca actaaaacca gatcagaggt taggggtagt tctaaaaagc cttggaagca aaaaggaacc ggtagagcta gggttggtac aaagcgaaat ccagtttgga ttggcggagg tatagcatta gggccaaagc ctagggatta tagctataga ttgcctaaaa aggtaaaaaa gcttgcattt aagtctgtat taagtttgcg tgctgctgat gaaaatagtt ttaaagttat tgagaatttt aatgttgaat ctggaaaaac gaaagatctt gctttaataa taaaaaattt tgcaagtttt aatggtaagg tggttattct tttgggcaat gatgatcaga tgataaaaag ggctggtaaa aatataagag atttaaagat tttatctttt gataaactta gggttgttga tttattttat gctaagaatt taatagctct agaatccgct gttaacaagc tcaatgagtt ttacattaaa taataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaagaaaa gttttttcta aagatgggaa agagattgga actataaatt tggatgatag agtttttaat atagaaatta gtcatgggtc tatttataat gctataaaaa acgagttgtc taatcttagg gttggaacat cttcaactaa aaccagatca gaggttaggg gtagttctaa aaagccttgg aagcaaaaag gaaccggtag agctagggtt ggtacaaagc gaaatccagt ttggattggc ggaggtatag cattagggcc aaagcctagg gattatagct atagattgcc taaaaaggta aaaaagcttg catttaagtc tgtattaagt ttgcgtgctg ctgatgaaaa tagttttaaa gttattgaga attttaatgt tgaatctgga aaaacgaaag atcttgcttt aataataaaa aattttgcaa gttttaatgg taaggtggtt attcttttgg gcaatgatga tcagatgata aaaagggctg gtaaaaatat aagagattta aagattttat cttttgataa acttagggtt gttgatttat tttatgctaa gaatttaata gctctagaat ccgctgttaa caagctcaat gagttttaca ttaaataaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa