BobuA.00444.a
glyceraldehyde 3-phosphate dehydrogenase (gap) (BB_0057)
| CENTER ID: | BobuA.00444.a |
| ORGANISM: | Borrelia burgdorferi B31 |
| ASSOCIATED DISEASE: | Lyme disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.00444.a.A1.GE26187 | Full length( BobuA.00444.a ) | 1 | 335 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.00444.a.A1.PW26245 | Full length( BobuA.00444.a ) | 1 | 335 |
Structures
3HJA
DEPOSITED: 5/21/2009
DETERMINATION: XRay
CLONE: BobuA.00444.a.A1.GE26187
PROTEIN: BobuA.00444.a.A1.PW26245
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126628 |
| PATRIC ID: | fig|224326.49.peg.455 |
| RefSeq: | NP_212191.1 |
| UniProt: | P46795 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLAINGFGR IGRNVFKIAF ERGIDIVAIN DLTDPKTLAH LLKYDSTFGV YNKKVESRDG AIVVDGREIK IIAERDPKNL PWAKLGIDVV IESTGVFSSA TSDKGGYLDH VNHAGAKKVI LTVPAKDEIK TIVLGVNDHD INSDLKAVSN ASCTTNCLAP LAKVLHESFG IEQGLMTTVH AYTNDQRILD LPHSDLRRAR AAALSIIPTS TGAAKAVGLV LPELKGKLNG TSMRVPVPTG SIVDLTVQLK KKDVTKEEIN SVLRKASETP ELKGILGYTE DPIVSSDIKG NSHSSIVDGL ETMVLENGFA KILSWYDNEF GYSTRVVDLA QKLVK
NT Sequence
ATGAAATTGG CTATTAATGG CTTTGGGCGT ATTGGTAGAA ATGTTTTTAA GATTGCTTTT GAAAGAGGAA TTGATATTGT CGCAATAAAT GATTTAACAG ATCCTAAAAC TCTTGCACAT CTTTTAAAGT ATGATTCGAC TTTTGGAGTA TATAATAAAA AAGTTGAGTC AAGAGATGGT GCCATTGTTG TGGATGGAAG AGAAATAAAA ATTATTGCTG AGAGAGACCC AAAAAATCTT CCTTGGGCAA AGCTTGGAAT TGATGTTGTA ATTGAGTCAA CAGGTGTTTT TTCATCAGCA ACTAGTGATA AAGGGGGATA TCTTGATCAT GTGAATCATG CTGGGGCTAA GAAAGTTATT TTAACAGTTC CTGCTAAAGA CGAGATTAAA ACAATAGTTC TTGGTGTAAA TGATCACGAT ATTAATTCTG ATTTAAAGGC TGTGTCAAAT GCTTCATGTA CAACCAATTG CTTGGCTCCT CTTGCAAAAG TACTTCATGA ATCATTTGGA ATTGAACAAG GACTTATGAC AACAGTTCAT GCTTATACTA ATGATCAAAG AATTTTGGAT CTTCCTCATT CTGATCTTAG ACGGGCAAGA GCTGCTGCAC TTTCTATTAT TCCAACTTCA ACCGGTGCTG CAAAAGCAGT TGGACTTGTT TTGCCCGAAC TTAAGGGTAA GTTGAATGGT ACTTCAATGA GGGTGCCTGT GCCAACAGGT TCAATAGTTG ACCTTACAGT TCAACTTAAG AAAAAGGATG TTACAAAAGA AGAGATTAAT TCTGTGCTCA GGAAGGCATC AGAAACGCCT GAGCTTAAAG GTATTTTGGG GTACACGGAA GATCCAATTG TATCTTCAGA TATAAAGGGG AATTCTCATT CTTCTATTGT TGATGGTCTT GAGACAATGG TTCTTGAAAA TGGATTTGCA AAGATATTGT CATGGTATGA CAATGAGTTT GGATATTCTA CAAGAGTGGT TGATCTTGCT CAAAAATTGG TAAAA
Details for BobuA.00444.a.A1.GE26187
| HARVESTED ON: | 2/9/2009 |
| SEQUENCED ON: | 4/8/2009 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKLAINGFG RIGRNVFKIA FERGIDIVAI NDLTDPKTLA HLLKYDSTFG VYNKKVESRD GAIVVDGREI KIIAERDPKN LPWAKLGIDV VIESTGVFSS ATSDKGGYLD HVNHAGAKKV ILTVPAKDEI KTIVLGVNDH DINSDLKAVS NASCTTNCLA PLAKVLHESF GIEQGLMTTV HAYTNDQRIL DLPHSDLRRA RAAALSIIPT STGAAKAVGL VLPELKGKLN GTSMRVPVPT GSIVDLTVQL KKKDVTKEEI NSVLRKASET PELKGILGYT EDPIVSSDIK GNSHSSIVDG LETMVLENGF AKILSWYDNE FGYSTRVVDL AQKLVK
Validated NT Sequence
ggagnnnnac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaaa ttggctatta atggctttgg gcgtattggt agaaatgttt ttaagattgc ttttgaaaga ggaattgata ttgtcgcaat aaatgattta acagatccta aaactcttgc acatctttta aagtatgatt cgacttttgg agtatataat aaaaaagttg agtcaagaga tggtgccatt gttgtggatg gaagagaaat aaaaattatt gctgagagag acccaaaaaa tcttccttgg gcaaagcttg gaattgatgt tgtaattgag tcaacaggtg ttttttcatc agcaactagt gataaagggg gatatcttga tcatgtgaat catgctgggg ctaagaaagt tattttaaca gttcctgcta aagacgagat taaaacaata gttcttggtg taaatgatca cgatattaat tctgatttaa aggctgtgtc aaatgcttca tgtacaacca attgcttggc tcctcttgca aaagtacttc atgaatcatt tggaattgaa caaggactta tgacaacagt tcatgcttat actaatgatc aaagaatttt ggatcttcct cattctgatc ttagacgggc aagagctgct gcactttcta ttattccaac ttcaaccggt gctgcaaaag cagttggact tgttttgccc gaacttaagg gtaagttgaa tggtacttca atgagggtgc ctgtgccaac aggttcaata gttgacctta cagttcaact taagaaaaag gatgttacaa aagaagagat taattctgtg ctcaggaagg catcagaaac gcctgagctt aaaggtattt tggggtacac ggaagatcca attgtatctt cagatataaa ggggaattct cattcttcta ttgttgatgg tcttgagaca atggttcttg aaaatggatt tgcaaagata ttgtcatggt atgacaatga gtttggatat tctacaagag tggttgatct tgctcaaaaa ttggtaaaat aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLAINGFG RIGRNVFKIA FERGIDIVAI NDLTDPKTLA HLLKYDSTFG VYNKKVESRD GAIVVDGREI KIIAERDPKN LPWAKLGIDV VIESTGVFSS ATSDKGGYLD HVNHAGAKKV ILTVPAKDEI KTIVLGVNDH DINSDLKAVS NASCTTNCLA PLAKVLHESF GIEQGLMTTV HAYTNDQRIL DLPHSDLRRA RAAALSIIPT STGAAKAVGL VLPELKGKLN GTSMRVPVPT GSIVDLTVQL KKKDVTKEEI NSVLRKASET PELKGILGYT EDPIVSSDIK GNSHSSIVDG LETMVLENGF AKILSWYDNE FGYSTRVVDL AQKLVK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaattggct attaatggct ttgggcgtat tggtagaaat gtttttaaga ttgcttttga aagaggaatt gatattgtcg caataaatga tttaacagat cctaaaactc ttgcacatct tttaaagtat gattcgactt ttggagtata taataaaaaa gttgagtcaa gagatggtgc cattgttgtg gatggaagag aaataaaaat tattgctgag agagacccaa aaaatcttcc ttgggcaaag cttggaattg atgttgtaat tgagtcaaca ggtgtttttt catcagcaac tagtgataaa gggggatatc ttgatcatgt gaatcatgct ggggctaaga aagttatttt aacagttcct gctaaagacg agattaaaac aatagttctt ggtgtaaatg atcacgatat taattctgat ttaaaggctg tgtcaaatgc ttcatgtaca accaattgct tggctcctct tgcaaaagta cttcatgaat catttggaat tgaacaagga cttatgacaa cagttcatgc ttatactaat gatcaaagaa ttttggatct tcctcattct gatcttagac gggcaagagc tgctgcactt tctattattc caacttcaac cggtgctgca aaagcagttg gacttgtttt gcccgaactt aagggtaagt tgaatggtac ttcaatgagg gtgcctgtgc caacaggttc aatagttgac cttacagttc aacttaagaa aaaggatgtt acaaaagaag agattaattc tgtgctcagg aaggcatcag aaacgcctga gcttaaaggt attttggggt acacggaaga tccaattgta tcttcagata taaaggggaa ttctcattct tctattgttg atggtcttga gacaatggtt cttgaaaatg gatttgcaaa gatattgtca tggtatgaca atgagtttgg atattctaca agagtggttg atcttgctca aaaattggta aaataaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BobuA.00444.a.A1.PW26245
| PURIFICATION DATe: | 3/16/2009 |
| CONCENTRATION: | 30.05mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 40 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKLAINGFG RIGRNVFKIA FERGIDIVAI NDLTDPKTLA HLLKYDSTFG VYNKKVESRD GAIVVDGREI KIIAERDPKN LPWAKLGIDV VIESTGVFSS ATSDKGGYLD HVNHAGAKKV ILTVPAKDEI KTIVLGVNDH DINSDLKAVS NASCTTNCLA PLAKVLHESF GIEQGLMTTV HAYTNDQRIL DLPHSDLRRA RAAALSIIPT STGAAKAVGL VLPELKGKLN GTSMRVPVPT GSIVDLTVQL KKKDVTKEEI NSVLRKASET PELKGILGYT EDPIVSSDIK GNSHSSIVDG LETMVLENGF AKILSWYDNE FGYSTRVVDL AQKLVK
Validated NT Sequence
ggagnnnnac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaaa ttggctatta atggctttgg gcgtattggt agaaatgttt ttaagattgc ttttgaaaga ggaattgata ttgtcgcaat aaatgattta acagatccta aaactcttgc acatctttta aagtatgatt cgacttttgg agtatataat aaaaaagttg agtcaagaga tggtgccatt gttgtggatg gaagagaaat aaaaattatt gctgagagag acccaaaaaa tcttccttgg gcaaagcttg gaattgatgt tgtaattgag tcaacaggtg ttttttcatc agcaactagt gataaagggg gatatcttga tcatgtgaat catgctgggg ctaagaaagt tattttaaca gttcctgcta aagacgagat taaaacaata gttcttggtg taaatgatca cgatattaat tctgatttaa aggctgtgtc aaatgcttca tgtacaacca attgcttggc tcctcttgca aaagtacttc atgaatcatt tggaattgaa caaggactta tgacaacagt tcatgcttat actaatgatc aaagaatttt ggatcttcct cattctgatc ttagacgggc aagagctgct gcactttcta ttattccaac ttcaaccggt gctgcaaaag cagttggact tgttttgccc gaacttaagg gtaagttgaa tggtacttca atgagggtgc ctgtgccaac aggttcaata gttgacctta cagttcaact taagaaaaag gatgttacaa aagaagagat taattctgtg ctcaggaagg catcagaaac gcctgagctt aaaggtattt tggggtacac ggaagatcca attgtatctt cagatataaa ggggaattct cattcttcta ttgttgatgg tcttgagaca atggttcttg aaaatggatt tgcaaagata ttgtcatggt atgacaatga gtttggatat tctacaagag tggttgatct tgctcaaaaa ttggtaaaat aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLAINGFG RIGRNVFKIA FERGIDIVAI NDLTDPKTLA HLLKYDSTFG VYNKKVESRD GAIVVDGREI KIIAERDPKN LPWAKLGIDV VIESTGVFSS ATSDKGGYLD HVNHAGAKKV ILTVPAKDEI KTIVLGVNDH DINSDLKAVS NASCTTNCLA PLAKVLHESF GIEQGLMTTV HAYTNDQRIL DLPHSDLRRA RAAALSIIPT STGAAKAVGL VLPELKGKLN GTSMRVPVPT GSIVDLTVQL KKKDVTKEEI NSVLRKASET PELKGILGYT EDPIVSSDIK GNSHSSIVDG LETMVLENGF AKILSWYDNE FGYSTRVVDL AQKLVK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATTGGCT ATTAATGGCT TTGGGCGTAT TGGTAGAAAT GTTTTTAAGA TTGCTTTTGA AAGAGGAATT GATATTGTCG CAATAAATGA TTTAACAGAT CCTAAAACTC TTGCACATCT TTTAAAGTAT GATTCGACTT TTGGAGTATA TAATAAAAAA GTTGAGTCAA GAGATGGTGC CATTGTTGTG GATGGAAGAG AAATAAAAAT TATTGCTGAG AGAGACCCAA AAAATCTTCC TTGGGCAAAG CTTGGAATTG ATGTTGTAAT TGAGTCAACA GGTGTTTTTT CATCAGCAAC TAGTGATAAA GGGGGATATC TTGATCATGT GAATCATGCT GGGGCTAAGA AAGTTATTTT AACAGTTCCT GCTAAAGACG AGATTAAAAC AATAGTTCTT GGTGTAAATG ATCACGATAT TAATTCTGAT TTAAAGGCTG TGTCAAATGC TTCATGTACA ACCAATTGCT TGGCTCCTCT TGCAAAAGTA CTTCATGAAT CATTTGGAAT TGAACAAGGA CTTATGACAA CAGTTCATGC TTATACTAAT GATCAAAGAA TTTTGGATCT TCCTCATTCT GATCTTAGAC GGGCAAGAGC TGCTGCACTT TCTATTATTC CAACTTCAAC CGGTGCTGCA AAAGCAGTTG GACTTGTTTT GCCCGAACTT AAGGGTAAGT TGAATGGTAC TTCAATGAGG GTGCCTGTGC CAACAGGTTC AATAGTTGAC CTTACAGTTC AACTTAAGAA AAAGGATGTT ACAAAAGAAG AGATTAATTC TGTGCTCAGG AAGGCATCAG AAACGCCTGA GCTTAAAGGT ATTTTGGGGT ACACGGAAGA TCCAATTGTA TCTTCAGATA TAAAGGGGAA TTCTCATTCT TCTATTGTTG ATGGTCTTGA GACAATGGTT CTTGAAAATG GATTTGCAAA GATATTGTCA TGGTATGACA ATGAGTTTGG ATATTCTACA AGAGTGGTTG ATCTTGCTCA AAAATTGGTA AAATAAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA