BobuA.00429.a

pyroP-fructose 6-P1-PP transferase (pfk) (BB_0727)

CENTER ID: BobuA.00429.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00429.a.B1.GE39060 full length 1 447
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1118
RefSeq: NP_212861.2
UniProt: O51669

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYRIKNENLD FKIDSLGECK QNNPLIDFYA SEGSSHFVNE KNKIKFSVYR NEDKGDRYED VLLEKAGPRE KIYFVPRHVK AAITTCGGLC PGFNDVIRSI VRTLWKIYGV RNIYGVKFGY QGLLPESNSP FINLNPDVVD DINKFGGTIL GSSRGGIKPV EIVDTLERMN INMIFNIGGD GTQKGSLLIA EEIEKRNLKI AVVGIPKTVD NDFMFVQKSF GFETAVEQAV AAVAGAHFEA NSAYNGIGLV KVMGRDSGFI AAHTALSSND VNFCLIPELD FDIEGPNGFL VHLERRLLEK ESLEEIPHAV ILIAEGAGQK YFDHFPKKKD DSGNLLYEDI GLYIKDKITE YFKAKNIQFT LKYIDPSYII RSSPANASDS LYCARLGSNA VHAAMAGKTK MLISLWSTKF VHIPIKMAVI DRNKVNPNGS FWRDVLSSTG QPISMKN
NT Sequence
gtgtatagaa ttaaaaatga aaatttagat tttaaaatag atagcttggg agaatgcaag caaaacaatc ctttgattga tttttatgct agtgagggtt cttcccattt tgttaatgaa aaaaataaaa tcaagtttag tgtatatagg aatgaggata agggagatag gtatgaagat gttcttttag aaaaagctgg gcctagagaa aaaatttatt ttgtgcccag gcatgttaaa gctgctatta ctacttgtgg tgggctttgt cctggtttta acgatgttat tcgctctatt gtgcgaactt tatggaaaat atatggggtt cgcaatattt atggagtaaa atttggatat caagggcttc ttcctgagtc aaattcacct tttattaatc ttaatccaga tgttgttgat gatattaata aatttggagg tactattctt ggttcttcaa ggggtggtat taagcctgtg gaaatagttg atactttaga gagaatgaac attaatatga tttttaacat tggcggggat ggtactcaaa aggggtctct tcttattgct gaggagatag aaaaaagaaa tttaaaaata gcagttgtgg gcattcctaa aaccgtagac aatgatttta tgtttgttca aaaatctttt ggatttgaga ctgctgtaga acaagctgtt gcagctgttg ctggtgctca ttttgaagct aatagtgctt ataatggcat tggacttgtt aaagttatgg gacgggattc tggctttatt gctgctcata ccgcactttc ttctaatgat gttaattttt gtttaatccc agaacttgat tttgacatag aaggtcctaa tggatttctt gttcatcttg aaagacgact tttagagaaa gaaagcttag aagagattcc ccatgcagta atattgatag cagaaggagc aggccaaaaa tattttgatc attttcctaa gaagaaagac gattctggga atttgcttta tgaggatatt gggctttata ttaaagataa aattacagaa tattttaaag caaaaaatat acaatttact cttaaataca ttgatcccag ctatattatt agaagttcac ctgctaatgc cagtgattcg ctttattgtg ctaggcttgg gtcgaatgct gtgcatgctg caatggctgg taagacaaaa atgttgatta gtttatggag tacaaaattt gttcatatac ctattaagat ggcagtaatt gacagaaata aggttaatcc aaatggttct ttttggagag atgttctttc aagtacgggg caaccaatta gcatgaagaa ttaa
Details for BobuA.00429.a.B1.GE39060
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 51kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV YRIKNENLDF KIDSLGECKQ NNPLIDFYAS EGSSHFVNEK NKIKFSVYRN EDKGDRYEDV LLEKAGPREK IYFVPRHVKA AITTCGGLCP GFNDVIRSIV RTLWKIYGVR NIYGVKFGYQ GLLPESNSPF INLNPDVVDD INKFGGTILG SSRGGIKPVE IVDTLERMNI NMIFNIGGDG TQKGSLLIAE EIEKRNLKIA VVGIPKTVDN DFMFVQKSFG FETAVEQAVA AVAGAHFEAN SAYNGIGLVK VMGRDSGFIA AHTALSSNDV NFCLIPELDF DIEGPNGFLV HLERRLLEKE SLEEIPHAVI LIAEGAGQKY FDHFPKKKDD SGNLLYEDIG LYIKDKITEY FKAKNIQFTL KYIDPSYIIR SSPANASDSL YCARLGSNAV HAAMAGKTKM LISLCSTKFV HIPIKMAVID RNKVNPNGSF WRDVLS
Validated NT Sequence
acttgaaaga acatctctcc aaaaagaacc atttggatta accttatttc tgtcaattac tgccatctta ataggtatat gaacaaattt tgtactacat aaactaatca acatttttgt cttaccagcc attgcagcat gcacagcatt cgacccaagc ctagcacaat aaagcgaatc actggcatta gcaggtgaac ttctaataat atagctggga tcaatgtatt taagagtaaa ttgtatattt tttgctttaa aatattctgt aattttatct ttaatataaa gcccaatatc ctcataaagc aaattcccag aatcgtcttt cttcttagga aaatgatcaa aatatttttg gcctgctcct tctgctatca atattactgc atggggaatc tcttctaagc tttctttctc taaaagtcgt ctttcaagat gaacaagaaa tccattagga ccttctatgt caaaatcaag ttctgggatt aaacaaaaat taacatcatt agaagaaagt gcggtatgag cagcaataaa gccagaatcc cgtcccataa ctttaacaag tccaatgcca ttataagcac tattagcttc aaaatgagca ccagcaacag ctgcaacagc ttgttctaca gcagtctcaa atccaaaaga tttttgaaca aacataaaat cattgtctac ggttttagga atgcccacaa ctgctatttt taaatttctt ttttctatct cctcagcaat aagaagagac cccttttgag taccatcccc gccaatgtta aaaatcatat taatgttcat tctctctaaa gtatcaacta tttccacagg cttaatacca ccccttgaag aaccaagaat agtacctcca aatttattaa tatcatcaac aacatctgga ttaagattaa taaaaggtga atttgactca ggaagaagcc cttgatatcc aaattttact ccataaatat tgcgaacccc atatattttc cataaagttc gcacaataga gcgaataaca tcgttaaaac caggacaaag cccaccacaa gtagtaatag cagctttaac atgcctgggc acaaaataaa ttttttctct aggcccagct ttttctaaaa gaacatcttc atacctatct cccttatcct cattcctata tacactaaac ttgattttat ttttttcatt aacaaaatgg gaagaaccct cactagcata aaaatcaatc aaaggattgt tttgcttgca ttctcccaag ctatctattt taaaatctaa attttcattt ttaattctat acaccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMY RIKNENLDFK IDSLGECKQN NPLIDFYASE GSSHFVNEKN KIKFSVYRNE DKGDRYEDVL LEKAGPREKI YFVPRHVKAA ITTCGGLCPG FNDVIRSIVR TLWKIYGVRN IYGVKFGYQG LLPESNSPFI NLNPDVVDDI NKFGGTILGS SRGGIKPVEI VDTLERMNIN MIFNIGGDGT QKGSLLIAEE IEKRNLKIAV VGIPKTVDND FMFVQKSFGF ETAVEQAVAA VAGAHFEANS AYNGIGLVKV MGRDSGFIAA HTALSSNDVN FCLIPELDFD IEGPNGFLVH LERRLLEKES LEEIPHAVIL IAEGAGQKYF DHFPKKKDDS GNLLYEDIGL YIKDKITEYF KAKNIQFTLK YIDPSYIIRS SPANASDSLY CARLGSNAVH AAMAGKTKML ISLWSTKFVH IPIKMAVIDR NKVNPNGSFW RDVLSSTGQP ISMKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtata gaattaaaaa tgaaaattta gattttaaaa tagatagctt gggagaatgc aagcaaaaca atcctttgat tgatttttat gctagtgagg gttcttccca ttttgttaat gaaaaaaata aaatcaagtt tagtgtatat aggaatgagg ataagggaga taggtatgaa gatgttcttt tagaaaaagc tgggcctaga gaaaaaattt attttgtgcc caggcatgtt aaagctgcta ttactacttg tggtgggctt tgtcctggtt ttaacgatgt tattcgctct attgtgcgaa ctttatggaa aatatatggg gttcgcaata tttatggagt aaaatttgga tatcaagggc ttcttcctga gtcaaattca ccttttatta atcttaatcc agatgttgtt gatgatatta ataaatttgg aggtactatt cttggttctt caaggggtgg tattaagcct gtggaaatag ttgatacttt agagagaatg aacattaata tgatttttaa cattggcggg gatggtactc aaaaggggtc tcttcttatt gctgaggaga tagaaaaaag aaatttaaaa atagcagttg tgggcattcc taaaaccgta gacaatgatt ttatgtttgt tcaaaaatct tttggatttg agactgctgt agaacaagct gttgcagctg ttgctggtgc tcattttgaa gctaatagtg cttataatgg cattggactt gttaaagtta tgggacggga ttctggcttt attgctgctc ataccgcact ttcttctaat gatgttaatt tttgtttaat cccagaactt gattttgaca tagaaggtcc taatggattt cttgttcatc ttgaaagacg acttttagag aaagaaagct tagaagagat tccccatgca gtaatattga tagcagaagg agcaggccaa aaatattttg atcattttcc taagaagaaa gacgattctg ggaatttgct ttatgaggat attgggcttt atattaaaga taaaattaca gaatatttta aagcaaaaaa tatacaattt actcttaaat acattgatcc cagctatatt attagaagtt cacctgctaa tgccagtgat tcgctttatt gtgctaggct tgggtcgaat gctgtgcatg ctgcaatggc tggtaagaca aaaatgttga ttagtttatg gagtacaaaa tttgttcata tacctattaa gatggcagta attgacagaa ataaggttaa tccaaatggt tctttttgga gagatgttct ttcaagtacg gggcaaccaa ttagcatgaa gaattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat