BobuA.00420.a

acetyl-CoA C-acetyltransferase (fadA) (BB_0109)

CENTER ID:	BobuA.00420.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00420.a.A1.GE26185	Full length( BobuA.00420.a )		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126757
PATRIC ID:	fig\|224326.49.peg.507
RefSeq:	NP_212243.1
UniProt:	O51136

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIKKVAIIDG LRTPNFKFGG SFKGLNIIDE SSKVVKALLE RNKLYKVDEV IIGNVISAGL GQNIARQIAL KSDLGDTVPA FSVNKVCGSG LKALELAFNS IALGDNDIVL AGGVEDLTNS PYLLPRKIRF DGLKFGNFGI EDSIQKDALI DSLNFISMGL TAENLSEKYR ITREMQDEFA YNSHVKALKA RELGYFEDEI YPLTVFDKKT NSSVTISSDE EIRDNLTLNK LASLNPVFKE SGTVTAGNSS SLNDGACFLI LASEERVKSL GLSPLAYIGG FKSVGLDPLY MGFGAYMAIE GIISKLSLNP SEIDLIEVNE AFAAQALSIE KALFKKYNIT SDIINVNGGA IALGHPFAVS GSRILLTLSR SMKMKNKTKG IASVCIGGGQ GISSFLYR

NT Sequence

GTGATAAAAA AGGTAGCTAT TATTGATGGA CTTAGAACAC CCAATTTCAA GTTTGGAGGT TCTTTTAAGG GTTTAAATAT TATTGATGAA TCTTCAAAAG TTGTTAAAGC TTTGCTTGAA AGAAATAAGT TATACAAAGT GGATGAGGTT ATTATTGGTA ATGTAATATC TGCGGGACTT GGTCAAAATA TTGCAAGACA AATTGCTTTA AAGTCTGACT TAGGCGATAC TGTGCCTGCA TTTAGCGTTA ATAAAGTTTG TGGTTCTGGG CTTAAGGCCT TGGAGCTTGC ATTTAATTCT ATTGCCCTTG GAGACAATGA TATTGTTTTA GCTGGAGGAG TTGAAGATTT AACCAATTCT CCTTATTTAT TACCTAGAAA GATTAGATTT GACGGTCTTA AATTTGGTAA TTTTGGAATT GAAGATTCAA TCCAAAAAGA CGCCTTAATA GATTCTCTGA ATTTTATTTC TATGGGGCTT ACGGCTGAAA ATCTTTCAGA AAAATATAGA ATAACAAGAG AAATGCAAGA TGAATTTGCA TATAATTCTC ATGTAAAGGC ATTAAAGGCA AGAGAGCTTG GATATTTTGA GGATGAAATT TATCCCCTGA CTGTTTTTGA TAAGAAGACC AACTCTAGCG TAACTATCTC AAGTGATGAG GAAATAAGAG ATAATTTAAC TTTAAATAAG CTAGCATCTC TTAATCCTGT TTTTAAAGAA TCAGGCACAG TAACTGCCGG AAATTCTTCA AGTTTAAATG ATGGTGCTTG TTTTTTAATT TTAGCAAGTG AGGAAAGAGT AAAAAGCTTG GGGTTAAGTC CATTAGCTTA TATTGGAGGA TTTAAAAGCG TGGGGCTTGA TCCACTTTAT ATGGGTTTTG GAGCTTATAT GGCTATTGAA GGCATTATTA GTAAATTAAG TTTAAATCCT AGCGAAATAG ATTTGATTGA GGTAAATGAG GCATTTGCAG CTCAAGCATT AAGCATTGAA AAGGCCTTGT TTAAAAAATA CAATATAACT AGCGATATTA TTAATGTAAA TGGTGGTGCT ATTGCTTTGG GGCATCCATT TGCCGTTAGC GGTTCAAGAA TTTTATTAAC ACTTTCTCGC TCTATGAAAA TGAAGAATAA AACAAAAGGG ATAGCATCTG TTTGTATTGG TGGCGGACAA GGAATTTCTA GTTTTTTGTA TAGA

Details for BobuA.00420.a.A1.GE26185

HARVESTED ON:	2/9/2009
SEQUENCED ON:	4/16/2009
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	94

Validated AA Sequence

MVIKKVAIID GLRTPNFKFG GSFKGLNIID ESSKVVKALL ERNKLYKVDE VIIGNVISAG LGQNIARQIA LKSDLGDTVP AFSVNKVCGS GLKALELAFN SIALGDNDIV LAGGVEDLTN SPYLLPRKIR FDGLKFGNFG IEDSIQKDAL IDSLNFISMG LTAENLSEKY RITREMQDEF AYNSHVKALK ARELXYFEXE XYPLTXFDKK TXSSVXISSD EEIRDNLTLN KLASLNPVFK ESXTVTAGNS SSLNDGACFL ILASEERVKS LGLSPXAYIG GFKSVGLDPL YMGFGAYMAI EGIISKLSLN PSEIDLIEVN EAFAAQALSI EKALFKKYNI TSDIINVNGG AIALGHPFAV SGSRILLTLS RSMKMKNKTK GIASVCIGGG QGISSFL

Validated NT Sequence

acaaaaaact agaaattcct tgtccgccac caatacaaac agatgctatc ccttttgttt tattcttcat tttcatagag cgagaaagtg ttaataaaat tcttgaaccg ctaacggcaa atggatgccc caaagcaata gcaccaccat ttacattaat aatatcgcta gttatattgt attttttaaa caaggccttt tcaatgctta atgcttgagc tgcaaatgcc tcatttacct caatcaaatc tatttcgcta ggatttaaac ttaatttact aataatgcct tcaatagcca tataagctcc aaaacccata taaagtggat caagccccac gcttttaaat cctccaatat aagcnaatgg acttaacccc aagcttttta ctctttcctc acttgctaaa attaaaaaac aagcaccatc atttaaactt gaagaatttc cggcagttac tgtgcnngat tctttaaaaa caggattaag agatgctagc ttatttaaag ttaaattatc tcttatttcc tcatcacttg agatagntac gctagagtng gtcttcttat caaaancagt cagnggataa anttcntcct caaaatatnc aagctctctt gcctttaatg cctttacatg agaattatat gcaaattcat cttgcatttc tcttgttatt ctatattttt ctgaaagatt ttcagccgta agccccatag aaataaaatt cagagaatct attaaggcgt ctttttggat tgaatcttca attccaaaat taccaaattt aagaccgtca aatctaatct ttctaggtaa taaataagga gaattggtta aatcttcaac tcctccagct aaaacaatat cattgtctcc aagggcaata gaattaaatg caagctccaa ggccttaagc ccagaaccac aaactttatt aacgctaaat gcaggcacag tatcgcctaa gtcagacttt aaagcaattt gtcttgcaat attttgacca agtcccgcag atattacatt accaataata acctcatcca ctttgtataa cttatttctt tcaagcaaag ctttaacaac ttttgaagat tcatcaataa tatttaaacc cttaaaagaa cctccaaact tgaaattggg tgttctaagt ccatcaataa tagctacctt ttttatcacc atcgaaccag gaccctgggt ctgagcttcc aggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVIKKVAII DGLRTPNFKF GGSFKGLNII DESSKVVKAL LERNKLYKVD EVIIGNVISA GLGQNIARQI ALKSDLGDTV PAFSVNKVCG SGLKALELAF NSIALGDNDI VLAGGVEDLT NSPYLLPRKI RFDGLKFGNF GIEDSIQKDA LIDSLNFISM GLTAENLSEK YRITREMQDE FAYNSHVKAL KARELGYFED EIYPLTVFDK KTNSSVTISS DEEIRDNLTL NKLASLNPVF KESGTVTAGN SSSLNDGACF LILASEERVK SLGLSPLAYI GGFKSVGLDP LYMGFGAYMA IEGIISKLSL NPSEIDLIEV NEAFAAQALS IEKALFKKYN ITSDIINVNG GAIALGHPFA VSGSRILLTL SRSMKMKNKT KGIASVCIGG GQGISSFLYR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgataaaa aaggtagcta ttattgatgg acttagaaca cccaatttca agtttggagg ttcttttaag ggtttaaata ttattgatga atcttcaaaa gttgttaaag ctttgcttga aagaaataag ttatacaaag tggatgaggt tattattggt aatgtaatat ctgcgggact tggtcaaaat attgcaagac aaattgcttt aaagtctgac ttaggcgata ctgtgcctgc atttagcgtt aataaagttt gtggttctgg gcttaaggcc ttggagcttg catttaattc tattgccctt ggagacaatg atattgtttt agctggagga gttgaagatt taaccaattc tccttattta ttacctagaa agattagatt tgacggtctt aaatttggta attttggaat tgaagattca atccaaaaag acgccttaat agattctctg aattttattt ctatggggct tacggctgaa aatctttcag aaaaatatag aataacaaga gaaatgcaag atgaatttgc atataattct catgtaaagg cattaaaggc aagagagctt ggatattttg aggatgaaat ttatcccctg actgtttttg ataagaagac caactctagc gtaactatct caagtgatga ggaaataaga gataatttaa ctttaaataa gctagcatct cttaatcctg tttttaaaga atcaggcaca gtaactgccg gaaattcttc aagtttaaat gatggtgctt gttttttaat tttagcaagt gaggaaagag taaaaagctt ggggttaagt ccattagctt atattggagg atttaaaagc gtggggcttg atccacttta tatgggtttt ggagcttata tggctattga aggcattatt agtaaattaa gtttaaatcc tagcgaaata gatttgattg aggtaaatga ggcatttgca gctcaagcat taagcattga aaaggccttg tttaaaaaat acaatataac tagcgatatt attaatgtaa atggtggtgc tattgctttg gggcatccat ttgccgttag cggttcaaga attttattaa cactttctcg ctctatgaaa atgaagaata aaacaaaagg gatagcatct gtttgtattg gtggcggaca aggaatttct agttttttgt atagatgaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa