SSGCID: Target Page

BobuA.00339.a

alanine racemase (alr) (BB_0160)

CENTER ID:	BobuA.00339.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00339.a.B1.GE39056	full length		1	372

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.557
RefSeq:	NP_212294.1
UniProt:	O51182

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYNNKTMGSN KTIIINLNNL EHNLNLIKNK IGEKEIVATL KGDAYGHGLI NIFKFLKSKN INYFGLSNIE DAKTLKKIDK NIKILMYIKV DKKEIKNLIK FELVPFVSDF EYLFLIEKEC ALQKNKIKVH LKIDIGMNRY GIKIDDALEI ATYIQNSKFL ELEGICSHLP SIENFKTTQK QIEQFLFFLE TLKQKNIHPK FVHISNSGHI INYKLNPQFN MVRPGLILYG YCQSLKNKKP ALNFKPVLSL FSKVIFIKNV KKGEKISYSG IFQAKEDMKI GIIPIGYFDG IPQNISNDFY FLINNKKCKI RGKVCMNLTI VEIPKDLKVK TGSKVEIVSE KLSIDEMSKF SKRSHYELLC NIGKYEKRKY LD

NT Sequence

atgtataata ataaaacaat gggtagtaac aaaacaataa taataaattt aaataattta gaacataact taaatttaat taaaaataaa attggcgaaa aagaaatagt ggccacctta aaaggtgacg catatggtca cggacttata aacatcttta aatttttaaa atcaaaaaac ataaattatt ttggactttc taatattgaa gatgctaaaa cacttaaaaa aattgataaa aatataaaaa tattaatgta cattaaggtg gataaaaaag aaattaaaaa tttgattaaa tttgagttag taccatttgt ttctgatttt gaatatctat ttttaataga aaaagaatgc gcattgcaaa aaaataaaat aaaagtccac ttaaaaattg atatcggaat gaatagatat ggaataaaaa tagatgatgc ccttgaaata gctacatata ttcaaaattc aaaatttcta gaactagaag ggatctgctc acatctccca tcaatagaaa actttaaaac cacacaaaag caaatagagc aattcttatt ttttttagaa acactaaaac aaaaaaatat tcatcccaaa tttgtccata tttctaattc agggcacatt attaactaca aactaaatcc acaatttaat atggtaagac caggtcttat tctttacggt tactgtcaat cgctaaaaaa caaaaaacct gctttaaatt ttaagcctgt attaagttta ttttcaaaag taatatttat caaaaatgtc aaaaagggtg aaaaaatttc atattcgggt atatttcaag ccaaagaaga tatgaaaata ggaattattc caatcggata ttttgatgga atacctcaaa atataagtaa tgatttttat tttttaataa acaataaaaa atgtaaaata agaggaaaag tttgcatgaa tctgacaata gtagaaattc ccaaagacct aaaagttaaa acaggttcaa aagtagaaat tgtatcagaa aaattaagta tagatgaaat gagtaaattt tctaaaagaa gtcattatga attactatgt aatataggaa aatacgaaaa gagaaaatac ttagattaa

Details for BobuA.00339.a.B1.GE39056

HARVESTED ON:	3/31/2015
SEQUENCED ON:	3/27/2015
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMY NNKTMGSNKT IIINLNNLEH NLNLIKNKIG EKEIVATLKG DAYGHGLINI FKFLKSKNIN YFGLSNIEDA KTLKKIDKNI KILMYIKVDK KEIKNLIKFE LVPFVSDFEY LFLIEKECAL QKNKIKVHLK IDIGMNRYGI KIDDALEIAT YIQNSKFLEL EGICSHLPSI ENFKTTQKQI EQFLFFLETL KQKNIHPKFV HISNSGHIIN YKLNPQFNMV RPGLILYGYC QSLKNKKPAL NFKPVLSLFS KVIFIKNVKK GEKISYSGIF QAKEDMKIGI IPIGYFDGIP QNISNDFYFL INNKKCKIRG KVCMNLTIVE IPKDLKVKTG SKVEIVSEKL SIDEMSKFSK RSHYELLCNI G

Validated NT Sequence

tttcctatat tacatagtaa ttcataatga cttcttttag aaaatttact catttcatct atacttaatt tttctgatac aatttctact tttgaacctg ttttaacttt taggtctttg ggaatttcta ctattgtcag attcatgcaa acttttcctc ttattttaca ttttttattg tttattaaaa aataaaaatc attacttata ttttgaggta ttccatcaaa atatccgatt ggaataattc ctattttcat atcttctttg gcttgaaata tacccgaata tgaaattttt tcaccctttt tgacattttt gataaatatt acttttgaaa ataaacttaa tacaggctta aaatttaaag caggtttttt gttttttagc gattgacagt aaccgtaaag aataagacct ggtcttacca tattaaattg tggatttagt ttgtagttaa taatgtgccc tgaattagaa atatggacaa atttgggatg aatatttttt tgttttagtg tttctaaaaa aaataagaat tgctctattt gcttttgtgt ggttttaaag ttttctattg atgggagatg tgagcagatc ccttctagtt ctagaaattt tgaattttga atatatgtag ctatttcaag ggcatcatct atttttattc catatctatt cattccgata tcaattttta agtggacttt tattttattt ttttgcaatg cgcattcttt ttctattaaa aatagatatt caaaatcaga aacaaatggt actaactcaa atttaatcaa atttttaatt tcttttttat ccaccttaat gtacattaat atttttatat ttttatcaat ttttttaagt gttttagcat cttcaatatt agaaagtcca aaataattta tgttttttga ttttaaaaat ttaaagatgt ttataagtcc gtgaccatat gcgtcacctt ttaaggtggc cactatttct ttttcgccaa ttttattttt aattaaattt aagttatgtt ctaaattatt taaatttatt attattgttt tgttactacc cattgtttta ttattataca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtata ataataaaac aatgggtagt aacaaaacaa taataataaa tttaaataat ttagaacata acttaaattt aattaaaaat aaaattggcg aaaaagaaat agtggccacc ttaaaaggtg acgcatatgg tcacggactt ataaacatct ttaaattttt aaaatcaaaa aacataaatt attttggact ttctaatatt gaagatgcta aaacacttaa aaaaattgat aaaaatataa aaatattaat gtacattaag gtggataaaa aagaaattaa aaatttgatt aaatttgagt tagtaccatt tgtttctgat tttgaatatc tatttttaat agaaaaagaa tgcgcattgc aaaaaaataa aataaaagtc cacttaaaaa ttgatatcgg aatgaataga tatggaataa aaatagatga tgcccttgaa atagctacat atattcaaaa ttcaaaattt ctagaactag aagggatctg ctcacatctc ccatcaatag aaaactttaa aaccacacaa aagcaaatag agcaattctt atttttttta gaaacactaa aacaaaaaaa tattcatccc aaatttgtcc atatttctaa ttcagggcac attattaact acaaactaaa tccacaattt aatatggtaa gaccaggtct tattctttac ggttactgtc aatcgctaaa aaacaaaaaa cctgctttaa attttaagcc tgtattaagt ttattttcaa aagtaatatt tatcaaaaat gtcaaaaagg gtgaaaaaat ttcatattcg ggtatatttc aagccaaaga agatatgaaa ataggaatta ttccaatcgg atattttgat ggaatacctc aaaatataag taatgatttt tattttttaa taaacaataa aaaatgtaaa ataagaggaa aagtttgcat gaatctgaca atagtagaaa ttcccaaaga cctaaaagtt aaaacaggtt caaaagtaga aattgtatca gaaaaattaa gtatagatga aatgagtaaa ttttctaaaa gaagtcatta tgaattacta tgtaatatag gaaaatacga aaagagaaaa tacttagatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat