BobuA.00314.a

pyridoxal kinase (pdxK) (BB_0768)

CENTER ID: BobuA.00314.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00314.a.B2.GE39055 mature protein 41 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1159
RefSeq: NP_212902.1
UniProt: O51709

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRILAMHDI SSMGRTSLTI CIPVISSFNM QVCPFVTAVL SASTAYKKFE IVDLTDHLEK FINIWKEQNE HFDILYTGFL GSEKQQITIE KIIKLIKFEK IVIDPVFADD GEIYPIFDNK IISGFRKIIK YANIITPNIT ELEMLSKSSK LNNKDDIIKA ILNLDTKATV VVTSVKRGNL LGNICYNPKN KEYSEFFLEG LEQNFSGTGD LFTSLLIGYL EKFETEQALE KTTKAIHLII KESIKENVSK KEGVRIENFL KNTF
NT Sequence
atgaaaagaa ttttagcaat gcatgatatt tcaagcatgg gaagaacatc tcttacaata tgcataccag taatatcttc gtttaatatg caagtttgtc cttttgtgac agctgtcctt tctgcttcca cagcttataa aaaatttgaa atagtggatt taaccgatca tttagaaaaa tttatcaata tatggaaaga acaaaatgag cactttgaca tactctatac cggatttctg ggaagcgaaa aacaacaaat aacaatagag aaaataatta aattaataaa atttgaaaaa attgtaattg atcctgtgtt tgctgacgat ggagaaattt accctatatt tgataataaa ataattagtg gatttagaaa aatcataaag tacgcaaaca taataacacc caatatcaca gaacttgaaa tgctaagcaa aagctcaaaa cttaacaaca aagatgatat cataaaagca atattaaatc ttgatacaaa agcgacggta gttgttacaa gcgttaaaag gggaaatctc ttgggaaaca tttgctacaa tcctaaaaac aaagaatact cggagttttt tttagaagga ttagaacaaa atttcagtgg aacaggagat ttatttacca gcttacttat aggatatttg gaaaaatttg aaacagagca agccttagaa aaaacaacaa aggctattca cctaataata aaagagtcaa ttaaagaaaa tgtttcaaaa aaagaagggg tccgaattga aaatttctta aaaaatacat tttga
Details for BobuA.00314.a.B2.GE39055
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS ASTAYKKFEI VDLTDHLEKF ISIWKEQNEH FDILYTGFLG SEKQQITIEK IIKLIKFEKI VIDPVFADDG EIYPIFDNKI ISGFRKIIKY ANIITPNITE LEMLSKSSKL NNKDDIIKAI LNLDTKATVV VTSVKRGNLL GNICYNPKSK EYSEFFLEGL EQNFSGTGDL FTSLLIGYLE KFETEQALEK TTKAIHLIIK ESIKENVSKK EGVRIENFLK NTF
Validated NT Sequence
atcttactca cttaaaatgt attttttaag aaattttcaa ttcggacccc ttcttttttt gaaacatttt ctttaattga ctcttttatt attaggtgaa tagcctttgt tgttttttct aaggcttgct ctgtttcaaa tttttccaaa tatcctataa gtaagctggt aaataaatct cctgttccac tgaaattttg ttctaatcct tctaaaaaaa actccgagta ttctttgctt ttaggattgt agcaaatgtt tcccaagaga tttccccttt taacgcttgt aacaactacc gtcgcttttg tatcaagatt taatattgct tttatgatat catctttgtt gttaagtttt gagcttttgc ttagcatttc aagttctgtg atattgggtg ttattatgtt tgcgtacttt atgatttttc taaatccact aattatttta ttatcaaata tagggtaaat ttctccatcg tcagcaaaca caggatcaat tacaattttt tcaaatttta ttaatttaat tattttctct attgttattt gttgtttttc gcttcccaga aatccggtat agagtatgtc aaagtgctca ttttgttctt tccatatact gataaatttt tctaaatgat cggttaaatc cactatttca aattttttat aagctgtgga agcagacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHSA STAYKKFEIV DLTDHLEKFI NIWKEQNEHF DILYTGFLGS EKQQITIEKI IKLIKFEKIV IDPVFADDGE IYPIFDNKII SGFRKIIKYA NIITPNITEL EMLSKSSKLN NKDDIIKAIL NLDTKATVVV TSVKRGNLLG NICYNPKNKE YSEFFLEGLE QNFSGTGDLF TSLLIGYLEK FETEQALEKT TKAIHLIIKE SIKENVSKKE GVRIENFLKN TF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctgctt ccacagctta taaaaaattt gaaatagtgg atttaaccga tcatttagaa aaatttatca atatatggaa agaacaaaat gagcactttg acatactcta taccggattt ctgggaagcg aaaaacaaca aataacaata gagaaaataa ttaaattaat aaaatttgaa aaaattgtaa ttgatcctgt gtttgctgac gatggagaaa tttaccctat atttgataat aaaataatta gtggatttag aaaaatcata aagtacgcaa acataataac acccaatatc acagaacttg aaatgctaag caaaagctca aaacttaaca acaaagatga tatcataaaa gcaatattaa atcttgatac aaaagcgacg gtagttgtta caagcgttaa aaggggaaat ctcttgggaa acatttgcta caatcctaaa aacaaagaat actcggagtt ttttttagaa ggattagaac aaaatttcag tggaacagga gatttattta ccagcttact tataggatat ttggaaaaat ttgaaacaga gcaagcctta gaaaaaacaa caaaggctat tcacctaata ataaaagagt caattaaaga aaatgtttca aaaaaagaag gggtccgaat tgaaaatttc ttaaaaaata cattttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat