BobuA.00194.a

outer surface protein B (ospB)

CENTER ID: BobuA.00194.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00194.a.A1.GE26167 Full length( BobuA.00194.a ) 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1534
RefSeq: NP_045689.1
UniProt: P17739

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLLIGFALA LALIGCAQKG AESIGSQKEN DLNLEDSSKK SHQNAKQDLP AVTEDSVSLF NGNKIFVSKE KNSSGKYDLR ATIDQVELKG TSDKNNGSGT LEGSKPDKSK VKLTVSADLN TVTLEAFDAS NQKISSKVTK KQGSITXETL KANKLDSKKL TRSNGTTLEY SQITDADNAT KAVETLKNSI KLEGSLVGGK TTVEIKEGTV TLKREIEKDG KVKVFLNDTA GSNKKTGKWE DSTSTLTISA DSKKTKDLVF LTDGTITVQQ YNTAGTSLEG SASEIKNLSE LKNALK
NT Sequence
ATGAGATTAT TAATAGGATT TGCTTTAGCG TTAGCTTTAA TAGGATGTGC ACAAAAAGGT GCTGAGTCAA TTGGTTCTCA AAAAGAAAAT GATCTAAACC TTGAAGACTC TAGTAAAAAA TCACATCAAA ACGCTAAACA AGACCTTCCT GCGGTGACAG AAGACTCAGT GTCTTTGTTT AATGGTAATA AAATTTTTGT AAGCAAAGAA AAAAATAGCT CCGGCAAATA TGATTTAAGA GCAACAATTG ATCAGGTTGA ACTTAAAGGA ACTTCCGATA AAAACAATGG TTCTGGAACC CTTGAAGGTT CAAAGCCTGA CAAGAGTAAA GTAAAATTAA CAGTTTCTGC TGATTTAAAC ACAGTAACCT TAGAAGCATT TGATGCCAGC AACCAAAAAA TTTCAAGTAA AGTTACTAAA AAACAGGGGT CAATAACAKA GGAAACTCTC AAAGCTAATA AATTAGACTC AAAGAAATTA ACAAGATCAA ACGGAACTAC ACTTGAATAC TCACAAATAA CAGATGCTGA CAATGCTACA AAAGCAGTAG AAACTCTAAA AAATAGCATT AAGCTTGAAG GAAGTCTTGT AGGCGGAAAA ACAACAGTGG AAATTAAAGA AGGTACTGTT ACTCTAAAAA GAGAAATTGA AAAAGATGGA AAAGTAAAAG TCTTTTTGAA TGACACTGCA GGTTCTAACA AAAAAACAGG TAAATGGGAA GACAGTACTA GCACTTTAAC AATTAGTGCT GACAGCAAAA AAACTAAAGA TTTGGTGTTC TTAACAGATG GTACAATTAC AGTACAACAA TACAACACAG CTGGAACCAG CCTAGAAGGA TCAGCAAGTG AAATTAAAAA TCTTTCAGAG CTTAAAAACG CTTTAAAA
Details for BobuA.00194.a.A1.GE26167
HARVESTED ON: 2/9/2009
SEQUENCED ON: 3/27/2012
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLLIGFAL ALALIGCAQK GAESIGSQKE NDLNLEDSSK KSHQNAKQDL PAVTEDSVSL FNGNKIFVSK EKNSSGKYDL RATIDQVELK GTSDKNNGSG TLEGSKPDKS KVKLTVSADL NTVTLEAFDA SNQKISSKVT KKQGSITEET LKANKLDSKK LTRSNGTTLE YSQITDADNA TKAVETLKNS IKLEGSLVVG KTTVKIKEGT VTLKREIEKD GKVKVFLNDT AGSNKKTGKW EDSTSTLTIS ADSKKTKDLV FLTDGTITVQ QYNTAGTSLX XSASEIKNLS
Validated NT Sequence
ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgagatta ttaataggat ttgctttagc gttagcttta ataggatgtg cacaaaaagg tgctgagtca attggttctc aaaaagaaaa tgatctaaac cttgaagact ctagtaaaaa atcacatcaa aacgctaaac aagaccttcc tgcggtgaca gaagactcag tgtctttgtt taatggtaat aaaatttttg taagcaaaga aaaaaatagc tccggcaaat atgatttaag agcaacaatt gatcaggttg aacttaaagg aacttccgat aaaaacaatg gttctggaac ccttgaaggt tcaaagcctg acaagagtaa agtaaaatta acagtttctg ctgatttaaa cacagtaacc ttagaagcat ttgatgccag caaccaaaaa atttcaagta aagttactaa aaaacagggg tcaataacag aggaaactct caaagctaat aaattagact caaagaaatt aacaagatca aacggaacta cacttgaata ctcacaaata acagatgctg acaatgctac aaaagcagta gaaactctaa aaaatagcat taagcttgaa ggaagtcttg tagtcggaaa aacaacagtg aaaattaaag aaggtactgt tactctaaaa agagaaattg aaaaagatgg aaaagtaaaa gtctttttga atgacactgc aggttctaac aaaaaaacag gtaaatggga agacagtact agcactttaa caattagtgc tgacagcaaa aaaactaaag atttggtgtt cttaacagat ggnacaatta cagtacaaca atacaacaca gctggaacca gcctagannn atcagcaagt gaaattaaaa atctttcana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLLIGFAL ALALIGCAQK GAESIGSQKE NDLNLEDSSK KSHQNAKQDL PAVTEDSVSL FNGNKIFVSK EKNSSGKYDL RATIDQVELK GTSDKNNGSG TLEGSKPDKS KVKLTVSADL NTVTLEAFDA SNQKISSKVT KKQGSITXET LKANKLDSKK LTRSNGTTLE YSQITDADNA TKAVETLKNS IKLEGSLVGG KTTVEIKEGT VTLKREIEKD GKVKVFLNDT AGSNKKTGKW EDSTSTLTIS ADSKKTKDLV FLTDGTITVQ QYNTAGTSLE GSASEIKNLS ELKNALK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagattatta ataggatttg ctttagcgtt agctttaata ggatgtgcac aaaaaggtgc tgagtcaatt ggttctcaaa aagaaaatga tctaaacctt gaagactcta gtaaaaaatc acatcaaaac gctaaacaag accttcctgc ggtgacagaa gactcagtgt ctttgtttaa tggtaataaa atttttgtaa gcaaagaaaa aaatagctcc ggcaaatatg atttaagagc aacaattgat caggttgaac ttaaaggaac ttccgataaa aacaatggtt ctggaaccct tgaaggttca aagcctgaca agagtaaagt aaaattaaca gtttctgctg atttaaacac agtaacctta gaagcatttg atgccagcaa ccaaaaaatt tcaagtaaag ttactaaaaa acaggggtca ataacakagg aaactctcaa agctaataaa ttagactcaa agaaattaac aagatcaaac ggaactacac ttgaatactc acaaataaca gatgctgaca atgctacaaa agcagtagaa actctaaaaa atagcattaa gcttgaagga agtcttgtag gcggaaaaac aacagtggaa attaaagaag gtactgttac tctaaaaaga gaaattgaaa aagatggaaa agtaaaagtc tttttgaatg acactgcagg ttctaacaaa aaaacaggta aatgggaaga cagtactagc actttaacaa ttagtgctga cagcaaaaaa actaaagatt tggtgttctt aacagatggt acaattacag tacaacaata caacacagct ggaaccagcc tagaaggatc agcaagtgaa attaaaaatc tttcagagct taaaaacgct ttaaaataaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a