BobuA.00177.a

heat shock protein 70 (dnaK-1)

CENTER ID: BobuA.00177.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00177.a.A1.GE26166 Full length( BobuA.00177.a ) 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.663
RefSeq: NP_212398.1
UniProt: O51279

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFMKKWIGID LGTTNTVASY FDVSSKIILN ERGERITPSI VSFSDKDVLV GSAAKNQILV NPQKTFYNFK TNIGSNNFYK VDGEFYKAEY ISAHLLASVK RNAEKFLDEE IENAVITVPA YFSEIQRRCV VEAANFAGLN CKAILNEPTA AAIAYAFERQ IDGIFLIYDL GGGTFDVTLM EKQGDTYTVL SVKGQSRLGG NDFNKIIEKY VLDSFKNKYP DFNLEDIFLL EQLRERIEEG KKNLSVMEEV DITLLFLDGK HLNYNLKRDE FNSMISEYID KTIQLSMECI ADSGVDISSV SKIILSGGST RIPLIEKVLK ESFPSATILD ALNQDEVVAI GAGIHAFSLS RNDSVIKFKD VTPYSLGLEI KDNEFFTLIK RNTALPISRS KLFTTTNDYQ DEIEIHILQG EYKKASLNYS IGRFSFGNIQ KALKGIPKIE VLFTLNESGI LSVNAKDLET NSSNFIEIKI TSSSDNVAKE SLSNTFTSII D
NT Sequence
GTGTTCATGA AAAAATGGAT AGGTATTGAT CTTGGAACCA CAAATACTGT TGCATCGTAT TTTGATGTTA GTTCTAAGAT AATATTAAAT GAAAGGGGTG AGCGAATAAC CCCTTCTATT GTTTCTTTCT CAGATAAGGA TGTTCTTGTT GGAAGTGCCG CTAAAAATCA AATATTAGTT AATCCTCAAA AAACATTTTA TAATTTTAAG ACCAATATAG GCTCTAATAA TTTTTATAAA GTAGATGGTG AATTTTATAA AGCAGAATAT ATTTCAGCAC ATTTGCTCGC TAGTGTTAAA AGGAACGCTG AAAAATTTTT AGATGAAGAG ATTGAAAATG CTGTAATAAC AGTCCCTGCC TATTTTTCTG AGATTCAAAG AAGATGTGTT GTTGAGGCTG CAAATTTTGC TGGTTTGAAT TGTAAGGCCA TACTTAATGA ACCAACCGCA GCTGCTATTG CTTATGCCTT TGAAAGACAG ATTGATGGAA TTTTTCTTAT TTATGATCTT GGGGGTGGAA CTTTTGATGT AACTCTTATG GAAAAACAAG GCGACACCTA TACTGTTCTT TCGGTCAAAG GGCAAAGTCG ACTTGGAGGT AATGACTTTA ATAAAATCAT TGAAAAGTAT GTTTTAGATA GTTTTAAAAA TAAATATCCT GATTTTAATC TAGAAGATAT TTTTCTTCTA GAACAGTTAA GAGAGCGAAT TGAAGAGGGT AAAAAAAATT TATCTGTTAT GGAAGAGGTC GACATTACTT TGCTTTTTCT TGATGGCAAG CATTTGAATT ATAATCTTAA AAGGGATGAA TTTAATTCAA TGATAAGTGA ATATATTGAC AAAACTATTC AGTTATCTAT GGAATGTATT GCTGATTCTG GGGTTGATAT TAGTAGTGTT TCAAAAATCA TACTTTCTGG TGGTTCAACA AGGATCCCCT TGATTGAAAA AGTTTTAAAG GAATCCTTTC CTTCTGCTAC AATTTTAGAT GCTTTAAATC AAGATGAAGT TGTAGCTATT GGCGCAGGTA TTCATGCTTT TAGTCTTTCT AGAAATGATT CTGTTATTAA GTTTAAGGAT GTAACTCCTT ATTCTCTTGG ACTTGAGATA AAGGATAATG AATTTTTTAC TCTAATAAAG AGAAATACTG CTTTGCCAAT TTCTAGAAGC AAACTATTTA CGACTACTAA TGATTATCAA GATGAAATTG AGATTCACAT ACTTCAAGGT GAATACAAAA AAGCCTCTTT AAATTATTCT ATAGGTAGGT TTTCCTTTGG CAATATTCAA AAAGCTTTAA AAGGAATTCC CAAAATTGAG GTACTTTTTA CTTTAAATGA AAGTGGTATT TTGAGTGTTA ATGCTAAAGA TTTAGAGACC AATTCTTCTA ATTTTATTGA AATAAAAATT ACAAGTTCAT CTGATAATGT AGCAAAAGAA AGTCTTTCAA ACACTTTTAC AAGTATTATT GAT
Details for BobuA.00177.a.A1.GE26166
HARVESTED ON: 2/9/2009
SEQUENCED ON: 3/27/2012
EXPECTED MW: 57kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFMKKWIG IDLGTTNTVA SYFDVSSKII LNERGERITP SIVSFSDKDV LVGSAAKNQI LVNPQKTFYN FKTNIGSNNF YKVDGEFYKA EYISAHLLAS VKRNAEKFLD EEIENAVITV PAYFSEIQRR CVVEAANFAG LNCKAILNEP TAAAIAYAFE RQIDGIFLIY DLGGGTFDVT LMEKQGDTYT VLSVKGQSRL GGNDFNKIIE KYVLDSFKNK YPDFNLEDIF LLEQLRERIE EGKKNLSVME EVDITLLFLD GKHLNYNLKR DEFNSMISEY IDKXIQLSME CIADSGVDIS SVSKIILSGG STRIPLIEKV XKXSFXXATI LDALNQDEVV AIGAGIHAFS LSRNDSVIKF KDVTPYSLGL EIKDNEFFTL IKRNTALPIS RSKLFTTTND YQDEIEIHIL QGEYKKASLN YSIGRFSFGN IQKALKGIPK IEVLFTLNES GILSVNAKDL ETNSSNFIEI KITSSSDNVA KESLSNTFTS IID
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt tcatgaaaaa atggataggt attgatcttg gaaccacaaa tactgttgca tcgtattttg atgttagttc taagataata ttaaatgaaa ggggtgagcg aataacccct tctattgttt ctttctcaga taaggatgtt cttgttggaa gtgccgctaa aaatcaaata ttagttaatc ctcaaaaaac attttataat tttaagacca atataggctc taataatttt tataaagtag atggtgaatt ttataaagca gaatatattt cagcacattt gctcgctagt gttaaaagga acgctgaaaa atttttagat gaagagattg aaaatgctgt aataacagtc cctgcctatt tttctgagat tcaaagaaga tgtgttgttg aggctgcaaa ttttgctggt ttgaattgta aggccatact taatgaacca accgcagctg ctattgctta tgcctttgaa agacagattg atggaatttt tcttatttat gatcttgggg gtggaacttt tgatgtaact cttatggaaa aacaaggcga cacctatact gttctttcgg tcaaagggca aagtcgactt ggaggtaatg actttaataa aatcattgaa aagtatgttt tagatagttt taaaaataaa tatcctgatt ttaatctaga agatattttt cttctagaac agttaagaga gcgaattgaa gagggtaaaa aaaatttatc tgttatggaa gaggtcgaca ttactttgct ttttcttgat ggcaagcatt tgaattataa tcttaaaagg gatgaattta attcaatgat aagtgaatat attgacaaan ctattcagtt atctatggaa tgtattgctg attctggggt tgatattagt agtgtttcaa aaatcatact ttctggtggt tcaacaagga tccccttgat tgaaaaagtt tnaaaggant cctttcnttn tgctacaatt ttagatgctt taaatcaaga tgaagtngta gctattggcg caggtattca tgcttttagt ctttctagaa atgattctgt tattaagttt aaggatgtaa ctccttattc tcttggactt gagataaagg ataatgaatt ttttactcta ataaagagaa atactgcttt gccaatttct agaagcaaac tatttacgac tactaatgat tatcaagatg aaattgagat tcacatactt caaggtgaat acaaaaaagc ctctttaaat tattctatag gtaggttttc ctttggcaat attcaaaaag ctttaaaagg aattcccaaa attgaggtac tttttacttt aaatgaaagt ggtattttga gtgttaatgc taaagattta gagaccaatt cttctaattt tattgaaata aaaattacaa gttcatctga taatgtagca aaagaaagtc tttcaaacac ttttacaagt attattgatt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFMKKWIG IDLGTTNTVA SYFDVSSKII LNERGERITP SIVSFSDKDV LVGSAAKNQI LVNPQKTFYN FKTNIGSNNF YKVDGEFYKA EYISAHLLAS VKRNAEKFLD EEIENAVITV PAYFSEIQRR CVVEAANFAG LNCKAILNEP TAAAIAYAFE RQIDGIFLIY DLGGGTFDVT LMEKQGDTYT VLSVKGQSRL GGNDFNKIIE KYVLDSFKNK YPDFNLEDIF LLEQLRERIE EGKKNLSVME EVDITLLFLD GKHLNYNLKR DEFNSMISEY IDKTIQLSME CIADSGVDIS SVSKIILSGG STRIPLIEKV LKESFPSATI LDALNQDEVV AIGAGIHAFS LSRNDSVIKF KDVTPYSLGL EIKDNEFFTL IKRNTALPIS RSKLFTTTND YQDEIEIHIL QGEYKKASLN YSIGRFSFGN IQKALKGIPK IEVLFTLNES GILSVNAKDL ETNSSNFIEI KITSSSDNVA KESLSNTFTS IID
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttcatg aaaaaatgga taggtattga tcttggaacc acaaatactg ttgcatcgta ttttgatgtt agttctaaga taatattaaa tgaaaggggt gagcgaataa ccccttctat tgtttctttc tcagataagg atgttcttgt tggaagtgcc gctaaaaatc aaatattagt taatcctcaa aaaacatttt ataattttaa gaccaatata ggctctaata atttttataa agtagatggt gaattttata aagcagaata tatttcagca catttgctcg ctagtgttaa aaggaacgct gaaaaatttt tagatgaaga gattgaaaat gctgtaataa cagtccctgc ctatttttct gagattcaaa gaagatgtgt tgttgaggct gcaaattttg ctggtttgaa ttgtaaggcc atacttaatg aaccaaccgc agctgctatt gcttatgcct ttgaaagaca gattgatgga atttttctta tttatgatct tgggggtgga acttttgatg taactcttat ggaaaaacaa ggcgacacct atactgttct ttcggtcaaa gggcaaagtc gacttggagg taatgacttt aataaaatca ttgaaaagta tgttttagat agttttaaaa ataaatatcc tgattttaat ctagaagata tttttcttct agaacagtta agagagcgaa ttgaagaggg taaaaaaaat ttatctgtta tggaagaggt cgacattact ttgctttttc ttgatggcaa gcatttgaat tataatctta aaagggatga atttaattca atgataagtg aatatattga caaaactatt cagttatcta tggaatgtat tgctgattct ggggttgata ttagtagtgt ttcaaaaatc atactttctg gtggttcaac aaggatcccc ttgattgaaa aagttttaaa ggaatccttt ccttctgcta caattttaga tgctttaaat caagatgaag ttgtagctat tggcgcaggt attcatgctt ttagtctttc tagaaatgat tctgttatta agtttaagga tgtaactcct tattctcttg gacttgagat aaaggataat gaatttttta ctctaataaa gagaaatact gctttgccaa tttctagaag caaactattt acgactacta atgattatca agatgaaatt gagattcaca tacttcaagg tgaatacaaa aaagcctctt taaattattc tataggtagg ttttcctttg gcaatattca aaaagcttta aaaggaattc ccaaaattga ggtacttttt actttaaatg aaagtggtat tttgagtgtt aatgctaaag atttagagac caattcttct aattttattg aaataaaaat tacaagttca tctgataatg tagcaaaaga aagtctttca aacactttta caagtattat tgattaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa