SSGCID: Target Page

BobuA.00158.a

glutamate racemase (murI) (BB_0100)

CENTER ID:	BobuA.00158.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.00158.a.B1.GE39052	full length		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.498
RefSeq:	NP_212234.1
UniProt:	O51127

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNFKEVIII FDSGIGGLSY FKYIKSRIGG CQYVYVADNK NFPYGEKSPE YLLEAVLFLI EKLKKIYNIG ALVLACNTIS VSVYNKLNFV FPVVYTLPDV SSVSDLVLKR VLLIATNTTL ESKFVKDQVN IHNDLIVKAA GELVNFVEYG ENYKKYALRC LEALKFEVVN TGREIVFLGC THYLHLKVMI EDFLKIPVYE NRELVVKNLI RSMNFSEHKG NYYKNDFDFV DDEFYLTENK NLTFYQNFCK KYNLRFKGMI V

NT Sequence

atgaaaaatt tcaaagaagt aataattatt tttgattcag gaataggagg gctttcttat tttaaatata ttaaaagtag aataggggga tgccaatatg tttatgttgc cgataataaa aatttccctt atggagaaaa aagtcctgaa tatcttctag aagcagtttt gtttttgatt gagaagctta aaaaaatcta taatattggt gcattagttt tggcttgtaa tacaatttct gttagtgtat acaataaatt aaattttgtt tttccagtag tctatacttt gccagatgta agttcagttt cagatcttgt tttaaaaaga gttcttttga ttgcaacaaa tactactctt gaaagcaaat ttgttaagga tcaagtaaat atacataatg atttgattgt aaaagctgct ggagagcttg ttaattttgt tgaatatgga gagaattaca aaaaatatgc tcttagatgt ttagaagctt taaaatttga agttgtaaat actggtagag aaattgtttt tcttggatgc acgcattatt tgcatcttaa ggtaatgata gaagattttt taaaaattcc tgtttatgag aatcgtgaat tagtggtaaa aaatcttatt agatcaatga atttttctga acacaaaggt aattattata agaatgattt tgattttgta gatgatgagt tttatttgac cgaaaataaa aatttgactt tttatcaaaa tttttgcaaa aaatataatc ttcgctttaa gggaatgata gtttga

Details for BobuA.00158.a.B1.GE39052

HARVESTED ON:	3/31/2015
SEQUENCED ON:	3/27/2015
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK NFKEVIIIFD SGIGGLSYFK YIKSRIGGCQ YVYVADNKNF PYGEKSPEYL LEAVLFLIEK LKKIYNIGAL VLACNTISVS VYNKLNFVFP VVYTLPDVSS VSDLVLKRVL LIATNTTLES KFVKDQVNIH NDLIVKAAGE LVNFVEYGEN YKKYALRCLE ALKFEVVNTG REIVFLGCTH YLHLKVMIED FLKIPVYENR ELVVKNLIRS MNFSEHKGNY YKNDFDFVDD EFYLTENKNL TFYQNFCKKY NLRFKGMIV

Validated NT Sequence

atcttactca cttaaactat cattccctta aagcgaagat tatatttttt gcaaaaattt tgataaaaag tcaaattttt attttcggtc aaataaaact catcatctac aaaatcaaaa tcattcttat aataattacc tttgtgttca gaaaaattca ttgatctaat aagatttttt accactaatt cacgattctc ataaacagga atttttaaaa aatcttctat cattacctta agatgcaaat aatgcgtgca tccaagaaaa acaatttctc taccagtatt tacaacttca aactttaaag cttctaaaca tctaagagca tattttttgt aattctctcc atattcaaca aaattaacaa gctctccagc agcttttaca atcaaatcat tatgtatatt tacttgatcc ttaacaaatt tgctttcaag agtagtattt gttgcaatca aaagaactct ttttaaaaca agatctgaaa ctgaacttac atctggcaaa gtatagacta ctggaaaaac aaaatttaat ttattgtata cactaacaga aattgtatta caagccaaaa ctaatgcacc aatattatag atttttttaa gcttctcaat caaaaacaaa actgcttcta gaagatattc aggacttttt tctccataag ggaaattttt attatcggca acataaacat attggcatcc ccctattcta cttttaatat atttaaaata agaaagccct cctattcctg aatcaaaaat aattattact tctttgaaat ttttcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa atttcaaaga agtaataatt atttttgatt caggaatagg agggctttct tattttaaat atattaaaag tagaataggg ggatgccaat atgtttatgt tgccgataat aaaaatttcc cttatggaga aaaaagtcct gaatatcttc tagaagcagt tttgtttttg attgagaagc ttaaaaaaat ctataatatt ggtgcattag ttttggcttg taatacaatt tctgttagtg tatacaataa attaaatttt gtttttccag tagtctatac tttgccagat gtaagttcag tttcagatct tgttttaaaa agagttcttt tgattgcaac aaatactact cttgaaagca aatttgttaa ggatcaagta aatatacata atgatttgat tgtaaaagct gctggagagc ttgttaattt tgttgaatat ggagagaatt acaaaaaata tgctcttaga tgtttagaag ctttaaaatt tgaagttgta aatactggta gagaaattgt ttttcttgga tgcacgcatt atttgcatct taaggtaatg atagaagatt ttttaaaaat tcctgtttat gagaatcgtg aattagtggt aaaaaatctt attagatcaa tgaatttttc tgaacacaaa ggtaattatt ataagaatga ttttgatttt gtagatgatg agttttattt gaccgaaaat aaaaatttga ctttttatca aaatttttgc aaaaaatata atcttcgctt taagggaatg atagtttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t