BobuA.00145.a

aspartyl-tRNA synthetase (BB_0446)

CENTER ID: BobuA.00145.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BobuA.00145.a.A1.GE25197 Full length( BobuA.00145.a ) 1 586
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BobuA.00145.a.A1.PW27472 Full length( BobuA.00145.a ) 1 586
BobuA.00145.a.A1.PW27473 Full length( BobuA.00145.a ) 1 586
BobuA.00145.a.A1.PW27474 Full length( BobuA.00145.a ) 1 586
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127662
PATRIC ID: fig|224326.49.peg.838
RefSeq: NP_212580.2
UniProt: O51402
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKVIKCNEL NEKLIDKKVE INAWVKKIRH HGKFIFLNIR DRYEKAQVLV NEEKLLKIAE KIKLEYCIKI QGLLIKRPPN MINANMKTGH FEILAKNIEI ISKCNELPFM IEDDNNASEN SKLEYRYLDL RRDSLKNKII LRCQATHLIR NFLIKRKFLE LETPTFVKST PEGARDFVIP SRIHKGSFYA LPQSPQLYKQ LIMIAGFDKY FQIARCYRDE DSRGDRQPEF TQLDLEMSFV KKENIFKLIE NMLFLIFKNC ININLPKKFK KITYKKAMNK YGSDKPDTRF ELELQDISRN LKNSEFNIFK DTLKNKGSIK ILIVKDKADK FSRAKINNLE EIAKLYKTQG LYFTKIENNK FSGGIAKFLK TEEQELIKTY SLENNDIIFF TANNNWETAC KAMGQIRIKI ANDLGLIDEN KFEFLWVYDF PLFEYDENTK TYSPAHHMFS LPKKQYIANL EKNPKKTIGE IYDLVLNGVE LGSGSIRIHN KELQQRIFKI IGFQKEKSED RFGFFLKALE YGAPNHGGIA IGIDRLIMLM TKSTSIKDVI LFPKNSFAAS PLDNSPSKIS NEQLKELGIN IVDGDN
NT Sequence
ATGTTTAAAG TTATCAAATG TAATGAATTG AATGAAAAAT TGATAGACAA AAAAGTTGAA ATAAATGCTT GGGTAAAAAA AATTAGACAC CACGGAAAAT TTATCTTTCT AAATATAAGA GACAGATATG AAAAAGCTCA AGTTCTGGTA AATGAAGAAA AGCTTCTAAA GATCGCAGAA AAAATAAAAC TTGAATATTG CATTAAAATT CAAGGACTGT TGATCAAAAG ACCCCCCAAC ATGATAAATG CAAATATGAA AACAGGACAT TTTGAAATAT TGGCAAAAAA CATTGAAATT ATCTCAAAGT GCAATGAATT GCCATTTATG ATAGAAGATG ACAATAATGC AAGTGAAAAC TCAAAACTTG AATACAGATA CTTAGATTTA AGAAGAGATT CCTTGAAAAA TAAAATTATT TTAAGATGTC AGGCTACTCA TCTTATTAGA AATTTTTTAA TAAAAAGAAA ATTTTTAGAG CTAGAAACTC CAACTTTTGT AAAATCAACG CCAGAAGGTG CAAGAGATTT TGTAATCCCA TCAAGGATTC ACAAAGGATC TTTTTATGCA CTACCTCAAT CTCCACAACT TTACAAACAA CTCATAATGA TAGCAGGATT TGACAAATAC TTTCAAATAG CCCGCTGCTA TAGAGACGAA GATTCAAGAG GGGACAGACA ACCAGAGTTC ACCCAGCTCG ATCTTGAAAT GAGCTTTGTC AAAAAAGAAA ATATTTTTAA ATTAATAGAA AATATGCTTT TTTTAATATT CAAAAATTGC ATCAATATTA ACCTACCTAA AAAATTCAAA AAAATAACAT ACAAAAAGGC AATGAACAAA TATGGAAGCG ACAAACCAGA TACTAGATTT GAACTTGAAT TACAAGATAT AAGTCGTAAT CTAAAAAATT CAGAATTTAA TATATTCAAA GATACTCTAA AAAACAAAGG TTCAATTAAA ATTTTAATAG TAAAAGATAA AGCTGACAAG TTTTCAAGAG CAAAAATAAA CAATTTAGAA GAAATTGCAA AGCTTTACAA AACACAAGGG CTTTATTTTA CAAAAATTGA AAACAATAAA TTTTCCGGGG GAATTGCAAA ATTTTTAAAA ACAGAAGAAC AGGAATTAAT AAAAACCTAT TCTTTAGAAA ATAATGACAT AATTTTCTTT ACAGCTAATA ATAACTGGGA AACTGCATGT AAAGCAATGG GTCAAATTAG AATAAAAATT GCAAATGATC TTGGACTAAT AGATGAAAAT AAATTTGAAT TTCTATGGGT CTATGATTTT CCACTATTTG AATATGACGA AAATACAAAA ACCTATTCAC CAGCTCACCA CATGTTCTCG CTTCCCAAAA AGCAATATAT TGCTAATTTA GAAAAAAATC CAAAAAAAAC TATAGGTGAA ATTTACGATC TTGTTTTAAA TGGCGTAGAA CTTGGCTCAG GTTCAATTAG AATACATAAC AAAGAGCTTC AACAAAGAAT TTTCAAAATA ATAGGATTTC AAAAAGAAAA ATCAGAAGAT AGATTTGGAT TTTTTCTAAA AGCATTAGAA TATGGAGCTC CTAATCATGG TGGCATTGCT ATTGGCATTG ATAGACTAAT AATGTTGATG ACAAAATCAA CTTCAATAAA AGATGTAATA CTGTTTCCTA AAAATTCTTT TGCAGCAAGC CCTCTTGATA ATTCCCCCTC TAAAATCTCA AATGAACAAC TCAAAGAACT GGGAATTAAT ATTGTTGATG GTGACAAT
Details for BobuA.00145.a.A1.GE25197
HARVESTED ON: 5/6/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 70kDa
OBSERVED MW: 71kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFKVIKCNE LNEKLIDKKV EINAWVKKIR HHGKFIFLNI RDRYEKAQVL VNEEKLLKIA EKIKLEYCIK IQGLLIKRPP NMINANMKTG HFEILAKNIE IISKCNELPF MIEDDNNASE NSKLEYRYLD LRRDSLKNKI ILRCQATHLI RNFLIKRKFL ELETPTFVKS TPEGARDFVI PSRIHKGSFY ALPQSPQLYK QLIMIAGFDK YFQIARCYRD EDSRGDRQPE FTQLDLEMSF VKKENIFKLI ENMLFLIFKN CININLPKKF KKITYKKAMN KYGSDKPDTR FELELXI
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtttaa agttatcaaa tgtaatgaat tgaatgaaaa attgatagac aaaaaagttg aaataaatgc ttgggtaaaa aaaattagac accacggaaa atttatcttt ctaaatataa gagacagata tgaaaaagct caagttctgg taaatgaaga aaagcttcta aagatcgcag aaaaaataaa acttgaatat tgcattaaaa ttcaaggact gttgatcaaa agacccccca acatgataaa tgcaaatatg aaaacaggac attttgaaat attggcaaaa aacattgaaa ttatctcaaa gtgcaatgaa ttgccattta tgatagaaga tgacaataat gcaagtgaaa actcaaaact tgaatacaga tacttagatt taagaagaga ttccttgaaa aataaaatta ttttaagatg tcaggctact catcttatta gaaatttttt aataaaaaga aaatttttag agctagaaac tccaactttt gtaaaatcaa cgccagaagg tgcaagagat tttgtaatcc catcaaggat tcacaaagga tctttttatg cactacctca atctccacaa ctttacaaac aactcataat gatagcagga tttgacaaat actttcaaat agcccgctgc tatagagacg aagattcaag aggggacaga caaccagagt tcacccagct cgatcttgaa atgagctttg tcaaaaaaga aaatattttt aaattaatag aaaatatgct ttttttaata ttcaaaaatt gcatcaatat taacctacct aaaaaattca aaaaaataac atacaaaaag gcaatgaaca aatatggaag cgacaaacca gatactagat ttgaacttga attananata taagtcgtaa tctaaaaaat tcaganntta atatattcaa agatactcta aaaaacaaag nntncaatta aaattttaat agtaaaagat aaagctgaca ngttttcaag agcaaaaata aacaatttag aagaaattgc aaagctttac aaaacacaag ggctttattt tacaaaaatt gaaaacnaat aaattttccg ggggaattgc aaaattttta aaaacagaag aacaggaatt aataaaaacc tattctttag aaaataatga cataattttc tttacagcta ataataactg ggaaactgca tgtaaagcaa tgggtcaaat tagaataaaa attgcaaatg atcttggact aatagatgaa aataaatttg aatttctatg ggtctatgat tttccactat ttgaatatga cgaaaataca aaaacctatt caccagctca ccacatgttc tcgcttccca aaaagcaata tattgctaat ttagaaaaaa atccaaaaaa aactataggt gaaatttacg atcttgtttt aaatggcgta gaacttggct caggttcaat tagaatacat aacaaagagc ttcaacaaag aattttcaaa ataataggat ttcaaaaaga aaaatcagaa gatagatttg gattttttct aaaagcatta gaatatggag ctcctaatca tggtggcatt gctattggca ttgatagact aataatgttg atgacaaaat caacttcaat aaaagatgta atactgtttc ctaaaaattc ttttgcagca agccctcttg ataattcccc ctctaaaatc tcaaatgaac aactcaaaga actgggaatt aatattgttg atggtg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKVIKCNE LNEKLIDKKV EINAWVKKIR HHGKFIFLNI RDRYEKAQVL VNEEKLLKIA EKIKLEYCIK IQGLLIKRPP NMINANMKTG HFEILAKNIE IISKCNELPF MIEDDNNASE NSKLEYRYLD LRRDSLKNKI ILRCQATHLI RNFLIKRKFL ELETPTFVKS TPEGARDFVI PSRIHKGSFY ALPQSPQLYK QLIMIAGFDK YFQIARCYRD EDSRGDRQPE FTQLDLEMSF VKKENIFKLI ENMLFLIFKN CININLPKKF KKITYKKAMN KYGSDKPDTR FELELQDISR NLKNSEFNIF KDTLKNKGSI KILIVKDKAD KFSRAKINNL EEIAKLYKTQ GLYFTKIENN KFSGGIAKFL KTEEQELIKT YSLENNDIIF FTANNNWETA CKAMGQIRIK IANDLGLIDE NKFEFLWVYD FPLFEYDENT KTYSPAHHMF SLPKKQYIAN LEKNPKKTIG EIYDLVLNGV ELGSGSIRIH NKELQQRIFK IIGFQKEKSE DRFGFFLKAL EYGAPNHGGI AIGIDRLIML MTKSTSIKDV ILFPKNSFAA SPLDNSPSKI SNEQLKELGI NIVDGDN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaaagtt atcaaatgta atgaattgaa tgaaaaattg atagacaaaa aagttgaaat aaatgcttgg gtaaaaaaaa ttagacacca cggaaaattt atctttctaa atataagaga cagatatgaa aaagctcaag ttctggtaaa tgaagaaaag cttctaaaga tcgcagaaaa aataaaactt gaatattgca ttaaaattca aggactgttg atcaaaagac cccccaacat gataaatgca aatatgaaaa caggacattt tgaaatattg gcaaaaaaca ttgaaattat ctcaaagtgc aatgaattgc catttatgat agaagatgac aataatgcaa gtgaaaactc aaaacttgaa tacagatact tagatttaag aagagattcc ttgaaaaata aaattatttt aagatgtcag gctactcatc ttattagaaa ttttttaata aaaagaaaat ttttagagct agaaactcca acttttgtaa aatcaacgcc agaaggtgca agagattttg taatcccatc aaggattcac aaaggatctt tttatgcact acctcaatct ccacaacttt acaaacaact cataatgata gcaggatttg acaaatactt tcaaatagcc cgctgctata gagacgaaga ttcaagaggg gacagacaac cagagttcac ccagctcgat cttgaaatga gctttgtcaa aaaagaaaat atttttaaat taatagaaaa tatgcttttt ttaatattca aaaattgcat caatattaac ctacctaaaa aattcaaaaa aataacatac aaaaaggcaa tgaacaaata tggaagcgac aaaccagata ctagatttga acttgaatta caagatataa gtcgtaatct aaaaaattca gaatttaata tattcaaaga tactctaaaa aacaaaggtt caattaaaat tttaatagta aaagataaag ctgacaagtt ttcaagagca aaaataaaca atttagaaga aattgcaaag ctttacaaaa cacaagggct ttattttaca aaaattgaaa acaataaatt ttccggggga attgcaaaat ttttaaaaac agaagaacag gaattaataa aaacctattc tttagaaaat aatgacataa ttttctttac agctaataat aactgggaaa ctgcatgtaa agcaatgggt caaattagaa taaaaattgc aaatgatctt ggactaatag atgaaaataa atttgaattt ctatgggtct atgattttcc actatttgaa tatgacgaaa atacaaaaac ctattcacca gctcaccaca tgttctcgct tcccaaaaag caatatattg ctaatttaga aaaaaatcca aaaaaaacta taggtgaaat ttacgatctt gttttaaatg gcgtagaact tggctcaggt tcaattagaa tacataacaa agagcttcaa caaagaattt tcaaaataat aggatttcaa aaagaaaaat cagaagatag atttggattt tttctaaaag cattagaata tggagctcct aatcatggtg gcattgctat tggcattgat agactaataa tgttgatgac aaaatcaact tcaataaaag atgtaatact gtttcctaaa aattcttttg cagcaagccc tcttgataat tccccctcta aaatctcaaa tgaacaactc aaagaactgg gaattaatat tgttgatggt gacaattaaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BobuA.00145.a.A1.PW27472
PURIFICATION DATe: 8/10/2009
CONCENTRATION: 11.06mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 50µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 52
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFKVIKCNE LNEKLIDKKV EINAWVKKIR HHGKFIFLNI RDRYEKAQVL VNEEKLLKIA EKIKLEYCIK IQGLLIKRPP NMINANMKTG HFEILAKNIE IISKCNELPF MIEDDNNASE NSKLEYRYLD LRRDSLKNKI ILRCQATHLI RNFLIKRKFL ELETPTFVKS TPEGARDFVI PSRIHKGSFY ALPQSPQLYK QLIMIAGFDK YFQIARCYRD EDSRGDRQPE FTQLDLEMSF VKKENIFKLI ENMLFLIFKN CININLPKKF KKITYKKAMN KYGSDKPDTR FELELXI
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtttaa agttatcaaa tgtaatgaat tgaatgaaaa attgatagac aaaaaagttg aaataaatgc ttgggtaaaa aaaattagac accacggaaa atttatcttt ctaaatataa gagacagata tgaaaaagct caagttctgg taaatgaaga aaagcttcta aagatcgcag aaaaaataaa acttgaatat tgcattaaaa ttcaaggact gttgatcaaa agacccccca acatgataaa tgcaaatatg aaaacaggac attttgaaat attggcaaaa aacattgaaa ttatctcaaa gtgcaatgaa ttgccattta tgatagaaga tgacaataat gcaagtgaaa actcaaaact tgaatacaga tacttagatt taagaagaga ttccttgaaa aataaaatta ttttaagatg tcaggctact catcttatta gaaatttttt aataaaaaga aaatttttag agctagaaac tccaactttt gtaaaatcaa cgccagaagg tgcaagagat tttgtaatcc catcaaggat tcacaaagga tctttttatg cactacctca atctccacaa ctttacaaac aactcataat gatagcagga tttgacaaat actttcaaat agcccgctgc tatagagacg aagattcaag aggggacaga caaccagagt tcacccagct cgatcttgaa atgagctttg tcaaaaaaga aaatattttt aaattaatag aaaatatgct ttttttaata ttcaaaaatt gcatcaatat taacctacct aaaaaattca aaaaaataac atacaaaaag gcaatgaaca aatatggaag cgacaaacca gatactagat ttgaacttga attananata taagtcgtaa tctaaaaaat tcaganntta atatattcaa agatactcta aaaaacaaag nntncaatta aaattttaat agtaaaagat aaagctgaca ngttttcaag agcaaaaata aacaatttag aagaaattgc aaagctttac aaaacacaag ggctttattt tacaaaaatt gaaaacnaat aaattttccg ggggaattgc aaaattttta aaaacagaag aacaggaatt aataaaaacc tattctttag aaaataatga cataattttc tttacagcta ataataactg ggaaactgca tgtaaagcaa tgggtcaaat tagaataaaa attgcaaatg atcttggact aatagatgaa aataaatttg aatttctatg ggtctatgat tttccactat ttgaatatga cgaaaataca aaaacctatt caccagctca ccacatgttc tcgcttccca aaaagcaata tattgctaat ttagaaaaaa atccaaaaaa aactataggt gaaatttacg atcttgtttt aaatggcgta gaacttggct caggttcaat tagaatacat aacaaagagc ttcaacaaag aattttcaaa ataataggat ttcaaaaaga aaaatcagaa gatagatttg gattttttct aaaagcatta gaatatggag ctcctaatca tggtggcatt gctattggca ttgatagact aataatgttg atgacaaaat caacttcaat aaaagatgta atactgtttc ctaaaaattc ttttgcagca agccctcttg ataattcccc ctctaaaatc tcaaatgaac aactcaaaga actgggaatt aatattgttg atggtg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKVIKCNE LNEKLIDKKV EINAWVKKIR HHGKFIFLNI RDRYEKAQVL VNEEKLLKIA EKIKLEYCIK IQGLLIKRPP NMINANMKTG HFEILAKNIE IISKCNELPF MIEDDNNASE NSKLEYRYLD LRRDSLKNKI ILRCQATHLI RNFLIKRKFL ELETPTFVKS TPEGARDFVI PSRIHKGSFY ALPQSPQLYK QLIMIAGFDK YFQIARCYRD EDSRGDRQPE FTQLDLEMSF VKKENIFKLI ENMLFLIFKN CININLPKKF KKITYKKAMN KYGSDKPDTR FELELQDISR NLKNSEFNIF KDTLKNKGSI KILIVKDKAD KFSRAKINNL EEIAKLYKTQ GLYFTKIENN KFSGGIAKFL KTEEQELIKT YSLENNDIIF FTANNNWETA CKAMGQIRIK IANDLGLIDE NKFEFLWVYD FPLFEYDENT KTYSPAHHMF SLPKKQYIAN LEKNPKKTIG EIYDLVLNGV ELGSGSIRIH NKELQQRIFK IIGFQKEKSE DRFGFFLKAL EYGAPNHGGI AIGIDRLIML MTKSTSIKDV ILFPKNSFAA SPLDNSPSKI SNEQLKELGI NIVDGDN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTTAAAGTT ATCAAATGTA ATGAATTGAA TGAAAAATTG ATAGACAAAA AAGTTGAAAT AAATGCTTGG GTAAAAAAAA TTAGACACCA CGGAAAATTT ATCTTTCTAA ATATAAGAGA CAGATATGAA AAAGCTCAAG TTCTGGTAAA TGAAGAAAAG CTTCTAAAGA TCGCAGAAAA AATAAAACTT GAATATTGCA TTAAAATTCA AGGACTGTTG ATCAAAAGAC CCCCCAACAT GATAAATGCA AATATGAAAA CAGGACATTT TGAAATATTG GCAAAAAACA TTGAAATTAT CTCAAAGTGC AATGAATTGC CATTTATGAT AGAAGATGAC AATAATGCAA GTGAAAACTC AAAACTTGAA TACAGATACT TAGATTTAAG AAGAGATTCC TTGAAAAATA AAATTATTTT AAGATGTCAG GCTACTCATC TTATTAGAAA TTTTTTAATA AAAAGAAAAT TTTTAGAGCT AGAAACTCCA ACTTTTGTAA AATCAACGCC AGAAGGTGCA AGAGATTTTG TAATCCCATC AAGGATTCAC AAAGGATCTT TTTATGCACT ACCTCAATCT CCACAACTTT ACAAACAACT CATAATGATA GCAGGATTTG ACAAATACTT TCAAATAGCC CGCTGCTATA GAGACGAAGA TTCAAGAGGG GACAGACAAC CAGAGTTCAC CCAGCTCGAT CTTGAAATGA GCTTTGTCAA AAAAGAAAAT ATTTTTAAAT TAATAGAAAA TATGCTTTTT TTAATATTCA AAAATTGCAT CAATATTAAC CTACCTAAAA AATTCAAAAA AATAACATAC AAAAAGGCAA TGAACAAATA TGGAAGCGAC AAACCAGATA CTAGATTTGA ACTTGAATTA CAAGATATAA GTCGTAATCT AAAAAATTCA GAATTTAATA TATTCAAAGA TACTCTAAAA AACAAAGGTT CAATTAAAAT TTTAATAGTA AAAGATAAAG CTGACAAGTT TTCAAGAGCA AAAATAAACA ATTTAGAAGA AATTGCAAAG CTTTACAAAA CACAAGGGCT TTATTTTACA AAAATTGAAA ACAATAAATT TTCCGGGGGA ATTGCAAAAT TTTTAAAAAC AGAAGAACAG GAATTAATAA AAACCTATTC TTTAGAAAAT AATGACATAA TTTTCTTTAC AGCTAATAAT AACTGGGAAA CTGCATGTAA AGCAATGGGT CAAATTAGAA TAAAAATTGC AAATGATCTT GGACTAATAG ATGAAAATAA ATTTGAATTT CTATGGGTCT ATGATTTTCC ACTATTTGAA TATGACGAAA ATACAAAAAC CTATTCACCA GCTCACCACA TGTTCTCGCT TCCCAAAAAG CAATATATTG CTAATTTAGA AAAAAATCCA AAAAAAACTA TAGGTGAAAT TTACGATCTT GTTTTAAATG GCGTAGAACT TGGCTCAGGT TCAATTAGAA TACATAACAA AGAGCTTCAA CAAAGAATTT TCAAAATAAT AGGATTTCAA AAAGAAAAAT CAGAAGATAG ATTTGGATTT TTTCTAAAAG CATTAGAATA TGGAGCTCCT AATCATGGTG GCATTGCTAT TGGCATTGAT AGACTAATAA TGTTGATGAC AAAATCAACT TCAATAAAAG ATGTAATACT GTTTCCTAAA AATTCTTTTG CAGCAAGCCC TCTTGATAAT TCCCCCTCTA AAATCTCAAA TGAACAACTC AAAGAACTGG GAATTAATAT TGTTGATGGT GACAATTAAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BobuA.00145.a.A1.PW27473
PURIFICATION DATe: 8/10/2009
CONCENTRATION: 12.72mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 50µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 52
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFKVIKCNE LNEKLIDKKV EINAWVKKIR HHGKFIFLNI RDRYEKAQVL VNEEKLLKIA EKIKLEYCIK IQGLLIKRPP NMINANMKTG HFEILAKNIE IISKCNELPF MIEDDNNASE NSKLEYRYLD LRRDSLKNKI ILRCQATHLI RNFLIKRKFL ELETPTFVKS TPEGARDFVI PSRIHKGSFY ALPQSPQLYK QLIMIAGFDK YFQIARCYRD EDSRGDRQPE FTQLDLEMSF VKKENIFKLI ENMLFLIFKN CININLPKKF KKITYKKAMN KYGSDKPDTR FELELXI
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtttaa agttatcaaa tgtaatgaat tgaatgaaaa attgatagac aaaaaagttg aaataaatgc ttgggtaaaa aaaattagac accacggaaa atttatcttt ctaaatataa gagacagata tgaaaaagct caagttctgg taaatgaaga aaagcttcta aagatcgcag aaaaaataaa acttgaatat tgcattaaaa ttcaaggact gttgatcaaa agacccccca acatgataaa tgcaaatatg aaaacaggac attttgaaat attggcaaaa aacattgaaa ttatctcaaa gtgcaatgaa ttgccattta tgatagaaga tgacaataat gcaagtgaaa actcaaaact tgaatacaga tacttagatt taagaagaga ttccttgaaa aataaaatta ttttaagatg tcaggctact catcttatta gaaatttttt aataaaaaga aaatttttag agctagaaac tccaactttt gtaaaatcaa cgccagaagg tgcaagagat tttgtaatcc catcaaggat tcacaaagga tctttttatg cactacctca atctccacaa ctttacaaac aactcataat gatagcagga tttgacaaat actttcaaat agcccgctgc tatagagacg aagattcaag aggggacaga caaccagagt tcacccagct cgatcttgaa atgagctttg tcaaaaaaga aaatattttt aaattaatag aaaatatgct ttttttaata ttcaaaaatt gcatcaatat taacctacct aaaaaattca aaaaaataac atacaaaaag gcaatgaaca aatatggaag cgacaaacca gatactagat ttgaacttga attananata taagtcgtaa tctaaaaaat tcaganntta atatattcaa agatactcta aaaaacaaag nntncaatta aaattttaat agtaaaagat aaagctgaca ngttttcaag agcaaaaata aacaatttag aagaaattgc aaagctttac aaaacacaag ggctttattt tacaaaaatt gaaaacnaat aaattttccg ggggaattgc aaaattttta aaaacagaag aacaggaatt aataaaaacc tattctttag aaaataatga cataattttc tttacagcta ataataactg ggaaactgca tgtaaagcaa tgggtcaaat tagaataaaa attgcaaatg atcttggact aatagatgaa aataaatttg aatttctatg ggtctatgat tttccactat ttgaatatga cgaaaataca aaaacctatt caccagctca ccacatgttc tcgcttccca aaaagcaata tattgctaat ttagaaaaaa atccaaaaaa aactataggt gaaatttacg atcttgtttt aaatggcgta gaacttggct caggttcaat tagaatacat aacaaagagc ttcaacaaag aattttcaaa ataataggat ttcaaaaaga aaaatcagaa gatagatttg gattttttct aaaagcatta gaatatggag ctcctaatca tggtggcatt gctattggca ttgatagact aataatgttg atgacaaaat caacttcaat aaaagatgta atactgtttc ctaaaaattc ttttgcagca agccctcttg ataattcccc ctctaaaatc tcaaatgaac aactcaaaga actgggaatt aatattgttg atggtg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKVIKCNE LNEKLIDKKV EINAWVKKIR HHGKFIFLNI RDRYEKAQVL VNEEKLLKIA EKIKLEYCIK IQGLLIKRPP NMINANMKTG HFEILAKNIE IISKCNELPF MIEDDNNASE NSKLEYRYLD LRRDSLKNKI ILRCQATHLI RNFLIKRKFL ELETPTFVKS TPEGARDFVI PSRIHKGSFY ALPQSPQLYK QLIMIAGFDK YFQIARCYRD EDSRGDRQPE FTQLDLEMSF VKKENIFKLI ENMLFLIFKN CININLPKKF KKITYKKAMN KYGSDKPDTR FELELQDISR NLKNSEFNIF KDTLKNKGSI KILIVKDKAD KFSRAKINNL EEIAKLYKTQ GLYFTKIENN KFSGGIAKFL KTEEQELIKT YSLENNDIIF FTANNNWETA CKAMGQIRIK IANDLGLIDE NKFEFLWVYD FPLFEYDENT KTYSPAHHMF SLPKKQYIAN LEKNPKKTIG EIYDLVLNGV ELGSGSIRIH NKELQQRIFK IIGFQKEKSE DRFGFFLKAL EYGAPNHGGI AIGIDRLIML MTKSTSIKDV ILFPKNSFAA SPLDNSPSKI SNEQLKELGI NIVDGDN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTTAAAGTT ATCAAATGTA ATGAATTGAA TGAAAAATTG ATAGACAAAA AAGTTGAAAT AAATGCTTGG GTAAAAAAAA TTAGACACCA CGGAAAATTT ATCTTTCTAA ATATAAGAGA CAGATATGAA AAAGCTCAAG TTCTGGTAAA TGAAGAAAAG CTTCTAAAGA TCGCAGAAAA AATAAAACTT GAATATTGCA TTAAAATTCA AGGACTGTTG ATCAAAAGAC CCCCCAACAT GATAAATGCA AATATGAAAA CAGGACATTT TGAAATATTG GCAAAAAACA TTGAAATTAT CTCAAAGTGC AATGAATTGC CATTTATGAT AGAAGATGAC AATAATGCAA GTGAAAACTC AAAACTTGAA TACAGATACT TAGATTTAAG AAGAGATTCC TTGAAAAATA AAATTATTTT AAGATGTCAG GCTACTCATC TTATTAGAAA TTTTTTAATA AAAAGAAAAT TTTTAGAGCT AGAAACTCCA ACTTTTGTAA AATCAACGCC AGAAGGTGCA AGAGATTTTG TAATCCCATC AAGGATTCAC AAAGGATCTT TTTATGCACT ACCTCAATCT CCACAACTTT ACAAACAACT CATAATGATA GCAGGATTTG ACAAATACTT TCAAATAGCC CGCTGCTATA GAGACGAAGA TTCAAGAGGG GACAGACAAC CAGAGTTCAC CCAGCTCGAT CTTGAAATGA GCTTTGTCAA AAAAGAAAAT ATTTTTAAAT TAATAGAAAA TATGCTTTTT TTAATATTCA AAAATTGCAT CAATATTAAC CTACCTAAAA AATTCAAAAA AATAACATAC AAAAAGGCAA TGAACAAATA TGGAAGCGAC AAACCAGATA CTAGATTTGA ACTTGAATTA CAAGATATAA GTCGTAATCT AAAAAATTCA GAATTTAATA TATTCAAAGA TACTCTAAAA AACAAAGGTT CAATTAAAAT TTTAATAGTA AAAGATAAAG CTGACAAGTT TTCAAGAGCA AAAATAAACA ATTTAGAAGA AATTGCAAAG CTTTACAAAA CACAAGGGCT TTATTTTACA AAAATTGAAA ACAATAAATT TTCCGGGGGA ATTGCAAAAT TTTTAAAAAC AGAAGAACAG GAATTAATAA AAACCTATTC TTTAGAAAAT AATGACATAA TTTTCTTTAC AGCTAATAAT AACTGGGAAA CTGCATGTAA AGCAATGGGT CAAATTAGAA TAAAAATTGC AAATGATCTT GGACTAATAG ATGAAAATAA ATTTGAATTT CTATGGGTCT ATGATTTTCC ACTATTTGAA TATGACGAAA ATACAAAAAC CTATTCACCA GCTCACCACA TGTTCTCGCT TCCCAAAAAG CAATATATTG CTAATTTAGA AAAAAATCCA AAAAAAACTA TAGGTGAAAT TTACGATCTT GTTTTAAATG GCGTAGAACT TGGCTCAGGT TCAATTAGAA TACATAACAA AGAGCTTCAA CAAAGAATTT TCAAAATAAT AGGATTTCAA AAAGAAAAAT CAGAAGATAG ATTTGGATTT TTTCTAAAAG CATTAGAATA TGGAGCTCCT AATCATGGTG GCATTGCTAT TGGCATTGAT AGACTAATAA TGTTGATGAC AAAATCAACT TCAATAAAAG ATGTAATACT GTTTCCTAAA AATTCTTTTG CAGCAAGCCC TCTTGATAAT TCCCCCTCTA AAATCTCAAA TGAACAACTC AAAGAACTGG GAATTAATAT TGTTGATGGT GACAATTAAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BobuA.00145.a.A1.PW27474
PURIFICATION DATe: 8/10/2009
CONCENTRATION: 14.33mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 52
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFKVIKCNE LNEKLIDKKV EINAWVKKIR HHGKFIFLNI RDRYEKAQVL VNEEKLLKIA EKIKLEYCIK IQGLLIKRPP NMINANMKTG HFEILAKNIE IISKCNELPF MIEDDNNASE NSKLEYRYLD LRRDSLKNKI ILRCQATHLI RNFLIKRKFL ELETPTFVKS TPEGARDFVI PSRIHKGSFY ALPQSPQLYK QLIMIAGFDK YFQIARCYRD EDSRGDRQPE FTQLDLEMSF VKKENIFKLI ENMLFLIFKN CININLPKKF KKITYKKAMN KYGSDKPDTR FELELXI
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtttaa agttatcaaa tgtaatgaat tgaatgaaaa attgatagac aaaaaagttg aaataaatgc ttgggtaaaa aaaattagac accacggaaa atttatcttt ctaaatataa gagacagata tgaaaaagct caagttctgg taaatgaaga aaagcttcta aagatcgcag aaaaaataaa acttgaatat tgcattaaaa ttcaaggact gttgatcaaa agacccccca acatgataaa tgcaaatatg aaaacaggac attttgaaat attggcaaaa aacattgaaa ttatctcaaa gtgcaatgaa ttgccattta tgatagaaga tgacaataat gcaagtgaaa actcaaaact tgaatacaga tacttagatt taagaagaga ttccttgaaa aataaaatta ttttaagatg tcaggctact catcttatta gaaatttttt aataaaaaga aaatttttag agctagaaac tccaactttt gtaaaatcaa cgccagaagg tgcaagagat tttgtaatcc catcaaggat tcacaaagga tctttttatg cactacctca atctccacaa ctttacaaac aactcataat gatagcagga tttgacaaat actttcaaat agcccgctgc tatagagacg aagattcaag aggggacaga caaccagagt tcacccagct cgatcttgaa atgagctttg tcaaaaaaga aaatattttt aaattaatag aaaatatgct ttttttaata ttcaaaaatt gcatcaatat taacctacct aaaaaattca aaaaaataac atacaaaaag gcaatgaaca aatatggaag cgacaaacca gatactagat ttgaacttga attananata taagtcgtaa tctaaaaaat tcaganntta atatattcaa agatactcta aaaaacaaag nntncaatta aaattttaat agtaaaagat aaagctgaca ngttttcaag agcaaaaata aacaatttag aagaaattgc aaagctttac aaaacacaag ggctttattt tacaaaaatt gaaaacnaat aaattttccg ggggaattgc aaaattttta aaaacagaag aacaggaatt aataaaaacc tattctttag aaaataatga cataattttc tttacagcta ataataactg ggaaactgca tgtaaagcaa tgggtcaaat tagaataaaa attgcaaatg atcttggact aatagatgaa aataaatttg aatttctatg ggtctatgat tttccactat ttgaatatga cgaaaataca aaaacctatt caccagctca ccacatgttc tcgcttccca aaaagcaata tattgctaat ttagaaaaaa atccaaaaaa aactataggt gaaatttacg atcttgtttt aaatggcgta gaacttggct caggttcaat tagaatacat aacaaagagc ttcaacaaag aattttcaaa ataataggat ttcaaaaaga aaaatcagaa gatagatttg gattttttct aaaagcatta gaatatggag ctcctaatca tggtggcatt gctattggca ttgatagact aataatgttg atgacaaaat caacttcaat aaaagatgta atactgtttc ctaaaaattc ttttgcagca agccctcttg ataattcccc ctctaaaatc tcaaatgaac aactcaaaga actgggaatt aatattgttg atggtg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKVIKCNE LNEKLIDKKV EINAWVKKIR HHGKFIFLNI RDRYEKAQVL VNEEKLLKIA EKIKLEYCIK IQGLLIKRPP NMINANMKTG HFEILAKNIE IISKCNELPF MIEDDNNASE NSKLEYRYLD LRRDSLKNKI ILRCQATHLI RNFLIKRKFL ELETPTFVKS TPEGARDFVI PSRIHKGSFY ALPQSPQLYK QLIMIAGFDK YFQIARCYRD EDSRGDRQPE FTQLDLEMSF VKKENIFKLI ENMLFLIFKN CININLPKKF KKITYKKAMN KYGSDKPDTR FELELQDISR NLKNSEFNIF KDTLKNKGSI KILIVKDKAD KFSRAKINNL EEIAKLYKTQ GLYFTKIENN KFSGGIAKFL KTEEQELIKT YSLENNDIIF FTANNNWETA CKAMGQIRIK IANDLGLIDE NKFEFLWVYD FPLFEYDENT KTYSPAHHMF SLPKKQYIAN LEKNPKKTIG EIYDLVLNGV ELGSGSIRIH NKELQQRIFK IIGFQKEKSE DRFGFFLKAL EYGAPNHGGI AIGIDRLIML MTKSTSIKDV ILFPKNSFAA SPLDNSPSKI SNEQLKELGI NIVDGDN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTTAAAGTT ATCAAATGTA ATGAATTGAA TGAAAAATTG ATAGACAAAA AAGTTGAAAT AAATGCTTGG GTAAAAAAAA TTAGACACCA CGGAAAATTT ATCTTTCTAA ATATAAGAGA CAGATATGAA AAAGCTCAAG TTCTGGTAAA TGAAGAAAAG CTTCTAAAGA TCGCAGAAAA AATAAAACTT GAATATTGCA TTAAAATTCA AGGACTGTTG ATCAAAAGAC CCCCCAACAT GATAAATGCA AATATGAAAA CAGGACATTT TGAAATATTG GCAAAAAACA TTGAAATTAT CTCAAAGTGC AATGAATTGC CATTTATGAT AGAAGATGAC AATAATGCAA GTGAAAACTC AAAACTTGAA TACAGATACT TAGATTTAAG AAGAGATTCC TTGAAAAATA AAATTATTTT AAGATGTCAG GCTACTCATC TTATTAGAAA TTTTTTAATA AAAAGAAAAT TTTTAGAGCT AGAAACTCCA ACTTTTGTAA AATCAACGCC AGAAGGTGCA AGAGATTTTG TAATCCCATC AAGGATTCAC AAAGGATCTT TTTATGCACT ACCTCAATCT CCACAACTTT ACAAACAACT CATAATGATA GCAGGATTTG ACAAATACTT TCAAATAGCC CGCTGCTATA GAGACGAAGA TTCAAGAGGG GACAGACAAC CAGAGTTCAC CCAGCTCGAT CTTGAAATGA GCTTTGTCAA AAAAGAAAAT ATTTTTAAAT TAATAGAAAA TATGCTTTTT TTAATATTCA AAAATTGCAT CAATATTAAC CTACCTAAAA AATTCAAAAA AATAACATAC AAAAAGGCAA TGAACAAATA TGGAAGCGAC AAACCAGATA CTAGATTTGA ACTTGAATTA CAAGATATAA GTCGTAATCT AAAAAATTCA GAATTTAATA TATTCAAAGA TACTCTAAAA AACAAAGGTT CAATTAAAAT TTTAATAGTA AAAGATAAAG CTGACAAGTT TTCAAGAGCA AAAATAAACA ATTTAGAAGA AATTGCAAAG CTTTACAAAA CACAAGGGCT TTATTTTACA AAAATTGAAA ACAATAAATT TTCCGGGGGA ATTGCAAAAT TTTTAAAAAC AGAAGAACAG GAATTAATAA AAACCTATTC TTTAGAAAAT AATGACATAA TTTTCTTTAC AGCTAATAAT AACTGGGAAA CTGCATGTAA AGCAATGGGT CAAATTAGAA TAAAAATTGC AAATGATCTT GGACTAATAG ATGAAAATAA ATTTGAATTT CTATGGGTCT ATGATTTTCC ACTATTTGAA TATGACGAAA ATACAAAAAC CTATTCACCA GCTCACCACA TGTTCTCGCT TCCCAAAAAG CAATATATTG CTAATTTAGA AAAAAATCCA AAAAAAACTA TAGGTGAAAT TTACGATCTT GTTTTAAATG GCGTAGAACT TGGCTCAGGT TCAATTAGAA TACATAACAA AGAGCTTCAA CAAAGAATTT TCAAAATAAT AGGATTTCAA AAAGAAAAAT CAGAAGATAG ATTTGGATTT TTTCTAAAAG CATTAGAATA TGGAGCTCCT AATCATGGTG GCATTGCTAT TGGCATTGAT AGACTAATAA TGTTGATGAC AAAATCAACT TCAATAAAAG ATGTAATACT GTTTCCTAAA AATTCTTTTG CAGCAAGCCC TCTTGATAAT TCCCCCTCTA AAATCTCAAA TGAACAACTC AAAGAACTGG GAATTAATAT TGTTGATGGT GACAATTAAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A