BobuA.00133.a
cysteinyl-tRNA synthetase (cysS) (BB_0599)
CENTER ID:
BobuA.00133.a
ORGANISM:
Borrelia burgdorferi B31
ASSOCIATED DISEASE:
Lyme disease
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
I
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BobuA.00133.a.A1.GE25195
Full length( BobuA.00133.a )
info
1
480
BobuA.00133.a.A1.GE25195
BobuA.00133.a.A1.GE25195
Full length( BobuA.00133.a )
info
1
480
BobuA.00133.a.A1.GE25195
BobuA.00133.a.B1.GE25291
Full length( BobuA.00133.a )
info
1
480
BobuA.00133.a.B1.GE25291
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BobuA.00133.a.B1.PS00097
Full length( BobuA.00133.a )
info
1
480
PS00097
BobuA.00133.a.A1.PW25233
Full length( BobuA.00133.a )
info
1
480
PW25233
BobuA.00133.a.A1.PW27585
Full length( BobuA.00133.a )
info
1
480
PW27585
Structures
3SP1
DEPOSITED: 6/30/2011
DETERMINATION: XRay
CLONE: BobuA.00133.a.A1.GE25195
PROTEIN: BobuA.00133.a.A1.PW27585
Publications by SSGCID
Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Clifton MC, Dranow DM, Edwards TE, Lorimer DD, Manoil C, Moen S, Myler PJ, Sankaran B, Sharma A, Staker BL, Van Voorhis WC
Sci Rep - 2017
volume 7, issue 1, pages 223
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILKLYNTRT KDFSELTNFE NVKVYACGPT VYNYAHIGNF RTYIFGDLLI KTLRFLGYKV NYAMNITDIG HLTGDLDDGE DKVAKTAREK GLTVYEISEF FTEAFFNDCR KLNIVYPDKV LVASKHIPIM IEVVKILEEK KITYFSNGNV YFDTSCFKSY GEMAGIDLID KDMTLPRVDV DKFKRNKTDF VLWFTNSKFK DQEMKWDSPW GFGYPSWHLE CAAMNLEYFK DALDIHLGGV DHIGVHHINE IAIAECFLNK KWCDVFVHGE FLIMDYNKMS KSRGNFITVK DLEDQNFSPL DFRYLCLTSH YRNQLKFSLD NLQASKIARE NLINKLSYFY ESLDPVDLNT LNKDLKNFGF SVEKEYYDSF VEKISFDLNV AQGLALLWEI IKSDNLSFVS KLRLAFIFDE IMSLNLREEI LKNLQNHDVV IDENMKALIE ERRIAKCEKN FKRADEIRDF FAKKGFVLVD TKEGTKVKRG
NT Sequence
ATGATTTTAA AGTTATATAA TACTAGAACA AAGGATTTTT CAGAATTAAC AAATTTTGAA AATGTTAAAG TGTATGCTTG CGGGCCTACT GTTTATAATT ATGCTCACAT CGGGAATTTT AGAACTTATA TTTTTGGAGA TTTGTTAATT AAAACTTTAA GGTTTTTGGG GTATAAAGTT AATTATGCGA TGAATATTAC AGATATTGGA CATTTAACAG GTGATCTTGA TGATGGAGAA GATAAGGTTG CTAAGACCGC AAGAGAGAAG GGCCTTACAG TTTATGAGAT TAGTGAATTT TTCACAGAGG CTTTTTTTAA CGATTGTAGA AAATTAAACA TTGTATATCC CGACAAAGTT CTTGTTGCAA GTAAACATAT TCCCATAATG ATAGAGGTTG TTAAAATTCT TGAAGAAAAA AAAATTACTT ATTTTTCTAA TGGTAATGTG TATTTTGATA CTTCTTGTTT TAAAAGCTAT GGTGAGATGG CCGGCATTGA TCTTATTGAT AAAGATATGA CTTTACCCAG AGTTGATGTT GATAAATTTA AAAGGAATAA AACCGATTTT GTTTTGTGGT TTACTAATTC TAAGTTTAAA GATCAGGAGA TGAAATGGGA TTCTCCTTGG GGATTTGGTT ATCCAAGTTG GCATTTGGAG TGCGCTGCGA TGAATTTGGA GTATTTCAAA GATGCTCTTG ATATTCATTT GGGAGGAGTT GATCATATTG GAGTTCACCA CATAAATGAA ATAGCAATAG CAGAGTGTTT TTTGAATAAA AAATGGTGTG ATGTCTTTGT TCATGGAGAA TTTTTGATTA TGGATTATAA TAAGATGTCA AAGTCACGTG GAAATTTTAT TACAGTTAAA GATTTAGAAG ATCAAAATTT TTCCCCTCTT GATTTTAGAT ATTTATGTTT GACATCGCAC TACAGGAACC AATTAAAATT TTCATTAGAT AATCTTCAAG CAAGCAAGAT TGCTAGAGAA AATTTGATAA ACAAGCTAAG TTATTTTTAT GAATCTTTAG ATCCAGTTGA TTTAAATACA CTTAATAAGG ATTTAAAAAA TTTTGGTTTT AGTGTAGAAA AAGAATATTA TGACTCTTTT GTAGAAAAAA TTTCTTTTGA TTTAAATGTT GCTCAAGGAT TGGCTTTGCT TTGGGAGATA ATTAAATCTG ACAATCTAAG CTTTGTTTCA AAGCTTAGAT TAGCTTTTAT TTTTGATGAG ATTATGTCAC TTAATCTGAG AGAAGAAATT TTAAAAAATT TACAAAACCA TGACGTAGTT ATTGATGAGA ATATGAAAGC TTTGATTGAA GAGAGAAGAA TAGCTAAATG TGAAAAAAAT TTTAAGCGTG CTGATGAAAT CAGAGATTTT TTTGCCAAAA AAGGTTTTGT TTTGGTTGAT ACTAAGGAAG GAACTAAGGT TAAAAGAGGC
Details for BobuA.00133.a.A1.GE25195
HARVESTED ON:
5/6/2008
SEQUENCED ON:
3/16/2012
EXPECTED MW:
58kDa
OBSERVED MW:
58kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BobuA.00133.a.A1.GE25195
HARVESTED ON:
5/6/2008
SEQUENCED ON:
3/16/2012
EXPECTED MW:
58kDa
OBSERVED MW:
58kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BobuA.00133.a.B1.GE25291
HARVESTED ON:
6/3/2008
SEQUENCED ON:
3/16/2012
EXPECTED MW:
57kDa
OBSERVED MW:
58kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BobuA.00133.a.B1.PS00097
PURIFICATION DATe:
10/5/2008
CONCENTRATION:
17.94mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
7
VIAL VOLUME:
100µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BobuA.00133.a.A1.PW25233
PURIFICATION DATe:
5/30/2008
CONCENTRATION:
26.9mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
8
VIAL VOLUME:
data unavailable
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for BobuA.00133.a.A1.PW27585
PURIFICATION DATe:
9/22/2009
CONCENTRATION:
25mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
4
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100