BobuA.00133.a

cysteinyl-tRNA synthetase (cysS) (BB_0599)

CENTER ID: BobuA.00133.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BobuA.00133.a.A1.GE25195 Full length( BobuA.00133.a ) 1 480
BobuA.00133.a.A1.GE25195 Full length( BobuA.00133.a ) 1 480
BobuA.00133.a.B1.GE25291 Full length( BobuA.00133.a ) 1 480
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BobuA.00133.a.B1.PS00097 Full length( BobuA.00133.a ) 1 480
BobuA.00133.a.A1.PW25233 Full length( BobuA.00133.a ) 1 480
BobuA.00133.a.A1.PW27585 Full length( BobuA.00133.a ) 1 480
Structures
3SP1
DEPOSITED: 6/30/2011
DETERMINATION: XRay
CLONE: BobuA.00133.a.A1.GE25195
PROTEIN: BobuA.00133.a.A1.PW27585
Publications by SSGCID
Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Clifton MC, Dranow DM, Edwards TE, Lorimer DD, Manoil C, Moen S, Myler PJ, Sankaran B, Sharma A, Staker BL, Van Voorhis WC
Sci Rep - 2017
volume 7, issue 1, pages 223
PMID: 28303005; PMCID: PMC5428304
PDB: 3SP1, 3TZE, 4E51, 4EX5, 4G6Z, 4GRI
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126755
PATRIC ID: fig|224326.49.peg.990
RefSeq: NP_212733.1
UniProt: O51545
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILKLYNTRT KDFSELTNFE NVKVYACGPT VYNYAHIGNF RTYIFGDLLI KTLRFLGYKV NYAMNITDIG HLTGDLDDGE DKVAKTAREK GLTVYEISEF FTEAFFNDCR KLNIVYPDKV LVASKHIPIM IEVVKILEEK KITYFSNGNV YFDTSCFKSY GEMAGIDLID KDMTLPRVDV DKFKRNKTDF VLWFTNSKFK DQEMKWDSPW GFGYPSWHLE CAAMNLEYFK DALDIHLGGV DHIGVHHINE IAIAECFLNK KWCDVFVHGE FLIMDYNKMS KSRGNFITVK DLEDQNFSPL DFRYLCLTSH YRNQLKFSLD NLQASKIARE NLINKLSYFY ESLDPVDLNT LNKDLKNFGF SVEKEYYDSF VEKISFDLNV AQGLALLWEI IKSDNLSFVS KLRLAFIFDE IMSLNLREEI LKNLQNHDVV IDENMKALIE ERRIAKCEKN FKRADEIRDF FAKKGFVLVD TKEGTKVKRG
NT Sequence
ATGATTTTAA AGTTATATAA TACTAGAACA AAGGATTTTT CAGAATTAAC AAATTTTGAA AATGTTAAAG TGTATGCTTG CGGGCCTACT GTTTATAATT ATGCTCACAT CGGGAATTTT AGAACTTATA TTTTTGGAGA TTTGTTAATT AAAACTTTAA GGTTTTTGGG GTATAAAGTT AATTATGCGA TGAATATTAC AGATATTGGA CATTTAACAG GTGATCTTGA TGATGGAGAA GATAAGGTTG CTAAGACCGC AAGAGAGAAG GGCCTTACAG TTTATGAGAT TAGTGAATTT TTCACAGAGG CTTTTTTTAA CGATTGTAGA AAATTAAACA TTGTATATCC CGACAAAGTT CTTGTTGCAA GTAAACATAT TCCCATAATG ATAGAGGTTG TTAAAATTCT TGAAGAAAAA AAAATTACTT ATTTTTCTAA TGGTAATGTG TATTTTGATA CTTCTTGTTT TAAAAGCTAT GGTGAGATGG CCGGCATTGA TCTTATTGAT AAAGATATGA CTTTACCCAG AGTTGATGTT GATAAATTTA AAAGGAATAA AACCGATTTT GTTTTGTGGT TTACTAATTC TAAGTTTAAA GATCAGGAGA TGAAATGGGA TTCTCCTTGG GGATTTGGTT ATCCAAGTTG GCATTTGGAG TGCGCTGCGA TGAATTTGGA GTATTTCAAA GATGCTCTTG ATATTCATTT GGGAGGAGTT GATCATATTG GAGTTCACCA CATAAATGAA ATAGCAATAG CAGAGTGTTT TTTGAATAAA AAATGGTGTG ATGTCTTTGT TCATGGAGAA TTTTTGATTA TGGATTATAA TAAGATGTCA AAGTCACGTG GAAATTTTAT TACAGTTAAA GATTTAGAAG ATCAAAATTT TTCCCCTCTT GATTTTAGAT ATTTATGTTT GACATCGCAC TACAGGAACC AATTAAAATT TTCATTAGAT AATCTTCAAG CAAGCAAGAT TGCTAGAGAA AATTTGATAA ACAAGCTAAG TTATTTTTAT GAATCTTTAG ATCCAGTTGA TTTAAATACA CTTAATAAGG ATTTAAAAAA TTTTGGTTTT AGTGTAGAAA AAGAATATTA TGACTCTTTT GTAGAAAAAA TTTCTTTTGA TTTAAATGTT GCTCAAGGAT TGGCTTTGCT TTGGGAGATA ATTAAATCTG ACAATCTAAG CTTTGTTTCA AAGCTTAGAT TAGCTTTTAT TTTTGATGAG ATTATGTCAC TTAATCTGAG AGAAGAAATT TTAAAAAATT TACAAAACCA TGACGTAGTT ATTGATGAGA ATATGAAAGC TTTGATTGAA GAGAGAAGAA TAGCTAAATG TGAAAAAAAT TTTAAGCGTG CTGATGAAAT CAGAGATTTT TTTGCCAAAA AAGGTTTTGT TTTGGTTGAT ACTAAGGAAG GAACTAAGGT TAAAAGAGGC
Details for BobuA.00133.a.A1.GE25195
HARVESTED ON: 5/6/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMILKLYNTR TKDFSELTNF ENVKVYACGP TVYNYAHIGN FRTYIFGDLL IKTLRFLGYK VNYAMNITDI GHLTGDLDDG EDKVAKTARE KGLTVYEISE FFTEAFFNDC RKLNIVYPDK VLVASKHIPI MIEVVKILEE KKITYFSNGN VYFDTSCFKS YGEMAGIDLI DKDMTLPRVD VDKFKRNKTD FVLWFTNSKF KDQEMKWDSP WGFGYPSWHL ECAAMNLEYF KDALDIHLGG VDHIGVHHIN EIAIAECFLN KKWCDVFVHG EFLIMDYNKM SKSRGNFITV KDLEDQNFSP LDFRYLCLTS HYRNQLKFSL DNLQASKIAR ENLINKLSYF YESLDPVDLN TLNKDLKNFG FSVEKEYYDS FVEKISFDLN VAQGLALLWE IIKSDNLSFV SKLRLAFIFD EIMSLNLREE ILKNLQNHDV VIDENMKALI EERRIAKCEK NFKRADEIRD FFAKKGFVLV DTKEGTKVKR G
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatttt aaagttatat aatactagaa caaaggattt ttcagaatta acaaattttg aaaatgttaa agtgtatgct tgcgggccta ctgtttataa ttatgctcac atcgggaatt ttagaactta tatttttgga gatttgttaa ttaaaacttt aaggtttttg gggtataaag ttaattatgc gatgaatatt acagatattg gacatttaac aggtgatctt gatgatggag aagataaggt tgctaagacc gcaagagaga agggccttac agtttatgag attagtgaat ttttcacaga ggcttttttt aacgattgta gaaaattaaa cattgtatat cccgacaaag ttcttgttgc aagtaaacat attcccataa tgatagaggt tgttaaaatt cttgaagaaa aaaaaattac ttatttttct aatggtaatg tgtattttga tacttcttgt tttaaaagct atggtgagat ggccggcatt gatcttattg ataaagatat gactttaccc agagttgatg ttgataaatt taaaaggaat aaaaccgatt ttgttttgtg gtttactaat tctaagttta aagatcagga gatgaaatgg gattctcctt ggggatttgg ttatccaagt tggcatttgg agtgcgctgc gatgaatttg gagtatttca aagatgctct tgatattcat ttgggaggag ttgatcatat tggagttcac cacataaatg aaatagcaat agcagagtgt tttttgaata aaaaatggtg tgatgtcttt gttcatggag aatttttgat tatggattat aataagatgt caaagtcacg tggaaatttt attacagtta aagatttaga agatcaaaat ttttcccctc ttgattttag atatttatgt ttgacatcgc actacaggaa ccaattaaaa ttttcattag ataatcttca agcaagcaag attgctagag aaaatttgat aaacaagcta agttattttt atgaatcttt agatccagtt gatttaaata cacttaataa ggatttaaaa aattttggtt ttagtgtaga aaaagaatat tatgactctt ttgtagaaaa aatttctttt gatttaaatg ttgctcaagg attggctttg ctttgggaga taattaaatc tgacaatcta agctttgttt caaagcttag attagctttt atttttgatg agattatgtc acttaatctg agagaagaaa ttttaaaaaa tttacaaaac catgacgtag ttattgatga gaatatgaaa gctttgattg aagagagaag aatagctaaa tgtgaaaaaa attttaagcg tgctgatgaa atcagagatt tttttgccaa aaaaggtttt gttttggttg atactaagga aggaactaag gttaaaagag gctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILKLYNTR TKDFSELTNF ENVKVYACGP TVYNYAHIGN FRTYIFGDLL IKTLRFLGYK VNYAMNITDI GHLTGDLDDG EDKVAKTARE KGLTVYEISE FFTEAFFNDC RKLNIVYPDK VLVASKHIPI MIEVVKILEE KKITYFSNGN VYFDTSCFKS YGEMAGIDLI DKDMTLPRVD VDKFKRNKTD FVLWFTNSKF KDQEMKWDSP WGFGYPSWHL ECAAMNLEYF KDALDIHLGG VDHIGVHHIN EIAIAECFLN KKWCDVFVHG EFLIMDYNKM SKSRGNFITV KDLEDQNFSP LDFRYLCLTS HYRNQLKFSL DNLQASKIAR ENLINKLSYF YESLDPVDLN TLNKDLKNFG FSVEKEYYDS FVEKISFDLN VAQGLALLWE IIKSDNLSFV SKLRLAFIFD EIMSLNLREE ILKNLQNHDV VIDENMKALI EERRIAKCEK NFKRADEIRD FFAKKGFVLV DTKEGTKVKR G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattttaaag ttatataata ctagaacaaa ggatttttca gaattaacaa attttgaaaa tgttaaagtg tatgcttgcg ggcctactgt ttataattat gctcacatcg ggaattttag aacttatatt tttggagatt tgttaattaa aactttaagg tttttggggt ataaagttaa ttatgcgatg aatattacag atattggaca tttaacaggt gatcttgatg atggagaaga taaggttgct aagaccgcaa gagagaaggg ccttacagtt tatgagatta gtgaattttt cacagaggct ttttttaacg attgtagaaa attaaacatt gtatatcccg acaaagttct tgttgcaagt aaacatattc ccataatgat agaggttgtt aaaattcttg aagaaaaaaa aattacttat ttttctaatg gtaatgtgta ttttgatact tcttgtttta aaagctatgg tgagatggcc ggcattgatc ttattgataa agatatgact ttacccagag ttgatgttga taaatttaaa aggaataaaa ccgattttgt tttgtggttt actaattcta agtttaaaga tcaggagatg aaatgggatt ctccttgggg atttggttat ccaagttggc atttggagtg cgctgcgatg aatttggagt atttcaaaga tgctcttgat attcatttgg gaggagttga tcatattgga gttcaccaca taaatgaaat agcaatagca gagtgttttt tgaataaaaa atggtgtgat gtctttgttc atggagaatt tttgattatg gattataata agatgtcaaa gtcacgtgga aattttatta cagttaaaga tttagaagat caaaattttt cccctcttga ttttagatat ttatgtttga catcgcacta caggaaccaa ttaaaatttt cattagataa tcttcaagca agcaagattg ctagagaaaa tttgataaac aagctaagtt atttttatga atctttagat ccagttgatt taaatacact taataaggat ttaaaaaatt ttggttttag tgtagaaaaa gaatattatg actcttttgt agaaaaaatt tcttttgatt taaatgttgc tcaaggattg gctttgcttt gggagataat taaatctgac aatctaagct ttgtttcaaa gcttagatta gcttttattt ttgatgagat tatgtcactt aatctgagag aagaaatttt aaaaaattta caaaaccatg acgtagttat tgatgagaat atgaaagctt tgattgaaga gagaagaata gctaaatgtg aaaaaaattt taagcgtgct gatgaaatca gagatttttt tgccaaaaaa ggttttgttt tggttgatac taaggaagga actaaggtta aaagaggcta aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BobuA.00133.a.A1.GE25195
HARVESTED ON: 5/6/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMILKLYNTR TKDFSELTNF ENVKVYACGP TVYNYAHIGN FRTYIFGDLL IKTLRFLGYK VNYAMNITDI GHLTGDLDDG EDKVAKTARE KGLTVYEISE FFTEAFFNDC RKLNIVYPDK VLVASKHIPI MIEVVKILEE KKITYFSNGN VYFDTSCFKS YGEMAGIDLI DKDMTLPRVD VDKFKRNKTD FVLWFTNSKF KDQEMKWDSP WGFGYPSWHL ECAAMNLEYF KDALDIHLGG VDHIGVHHIN EIAIAECFLN KKWCDVFVHG EFLIMDYNKM SKSRGNFITV KDLEDQNFSP LDFRYLCLTS HYRNQLKFSL DNLQASKIAR ENLINKLSYF YESLDPVDLN TLNKDLKNFG FSVEKEYYDS FVEKISFDLN VAQGLALLWE IIKSDNLSFV SKLRLAFIFD EIMSLNLREE ILKNLQNHDV VIDENMKALI EERRIAKCEK NFKRADEIRD FFAKKGFVLV DTKEGTKVKR G
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatttt aaagttatat aatactagaa caaaggattt ttcagaatta acaaattttg aaaatgttaa agtgtatgct tgcgggccta ctgtttataa ttatgctcac atcgggaatt ttagaactta tatttttgga gatttgttaa ttaaaacttt aaggtttttg gggtataaag ttaattatgc gatgaatatt acagatattg gacatttaac aggtgatctt gatgatggag aagataaggt tgctaagacc gcaagagaga agggccttac agtttatgag attagtgaat ttttcacaga ggcttttttt aacgattgta gaaaattaaa cattgtatat cccgacaaag ttcttgttgc aagtaaacat attcccataa tgatagaggt tgttaaaatt cttgaagaaa aaaaaattac ttatttttct aatggtaatg tgtattttga tacttcttgt tttaaaagct atggtgagat ggccggcatt gatcttattg ataaagatat gactttaccc agagttgatg ttgataaatt taaaaggaat aaaaccgatt ttgttttgtg gtttactaat tctaagttta aagatcagga gatgaaatgg gattctcctt ggggatttgg ttatccaagt tggcatttgg agtgcgctgc gatgaatttg gagtatttca aagatgctct tgatattcat ttgggaggag ttgatcatat tggagttcac cacataaatg aaatagcaat agcagagtgt tttttgaata aaaaatggtg tgatgtcttt gttcatggag aatttttgat tatggattat aataagatgt caaagtcacg tggaaatttt attacagtta aagatttaga agatcaaaat ttttcccctc ttgattttag atatttatgt ttgacatcgc actacaggaa ccaattaaaa ttttcattag ataatcttca agcaagcaag attgctagag aaaatttgat aaacaagcta agttattttt atgaatcttt agatccagtt gatttaaata cacttaataa ggatttaaaa aattttggtt ttagtgtaga aaaagaatat tatgactctt ttgtagaaaa aatttctttt gatttaaatg ttgctcaagg attggctttg ctttgggaga taattaaatc tgacaatcta agctttgttt caaagcttag attagctttt atttttgatg agattatgtc acttaatctg agagaagaaa ttttaaaaaa tttacaaaac catgacgtag ttattgatga gaatatgaaa gctttgattg aagagagaag aatagctaaa tgtgaaaaaa attttaagcg tgctgatgaa atcagagatt tttttgccaa aaaaggtttt gttttggttg atactaagga aggaactaag gttaaaagag gctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILKLYNTR TKDFSELTNF ENVKVYACGP TVYNYAHIGN FRTYIFGDLL IKTLRFLGYK VNYAMNITDI GHLTGDLDDG EDKVAKTARE KGLTVYEISE FFTEAFFNDC RKLNIVYPDK VLVASKHIPI MIEVVKILEE KKITYFSNGN VYFDTSCFKS YGEMAGIDLI DKDMTLPRVD VDKFKRNKTD FVLWFTNSKF KDQEMKWDSP WGFGYPSWHL ECAAMNLEYF KDALDIHLGG VDHIGVHHIN EIAIAECFLN KKWCDVFVHG EFLIMDYNKM SKSRGNFITV KDLEDQNFSP LDFRYLCLTS HYRNQLKFSL DNLQASKIAR ENLINKLSYF YESLDPVDLN TLNKDLKNFG FSVEKEYYDS FVEKISFDLN VAQGLALLWE IIKSDNLSFV SKLRLAFIFD EIMSLNLREE ILKNLQNHDV VIDENMKALI EERRIAKCEK NFKRADEIRD FFAKKGFVLV DTKEGTKVKR G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattttaaag ttatataata ctagaacaaa ggatttttca gaattaacaa attttgaaaa tgttaaagtg tatgcttgcg ggcctactgt ttataattat gctcacatcg ggaattttag aacttatatt tttggagatt tgttaattaa aactttaagg tttttggggt ataaagttaa ttatgcgatg aatattacag atattggaca tttaacaggt gatcttgatg atggagaaga taaggttgct aagaccgcaa gagagaaggg ccttacagtt tatgagatta gtgaattttt cacagaggct ttttttaacg attgtagaaa attaaacatt gtatatcccg acaaagttct tgttgcaagt aaacatattc ccataatgat agaggttgtt aaaattcttg aagaaaaaaa aattacttat ttttctaatg gtaatgtgta ttttgatact tcttgtttta aaagctatgg tgagatggcc ggcattgatc ttattgataa agatatgact ttacccagag ttgatgttga taaatttaaa aggaataaaa ccgattttgt tttgtggttt actaattcta agtttaaaga tcaggagatg aaatgggatt ctccttgggg atttggttat ccaagttggc atttggagtg cgctgcgatg aatttggagt atttcaaaga tgctcttgat attcatttgg gaggagttga tcatattgga gttcaccaca taaatgaaat agcaatagca gagtgttttt tgaataaaaa atggtgtgat gtctttgttc atggagaatt tttgattatg gattataata agatgtcaaa gtcacgtgga aattttatta cagttaaaga tttagaagat caaaattttt cccctcttga ttttagatat ttatgtttga catcgcacta caggaaccaa ttaaaatttt cattagataa tcttcaagca agcaagattg ctagagaaaa tttgataaac aagctaagtt atttttatga atctttagat ccagttgatt taaatacact taataaggat ttaaaaaatt ttggttttag tgtagaaaaa gaatattatg actcttttgt agaaaaaatt tcttttgatt taaatgttgc tcaaggattg gctttgcttt gggagataat taaatctgac aatctaagct ttgtttcaaa gcttagatta gcttttattt ttgatgagat tatgtcactt aatctgagag aagaaatttt aaaaaattta caaaaccatg acgtagttat tgatgagaat atgaaagctt tgattgaaga gagaagaata gctaaatgtg aaaaaaattt taagcgtgct gatgaaatca gagatttttt tgccaaaaaa ggttttgttt tggttgatac taaggaagga actaaggtta aaagaggcta aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BobuA.00133.a.B1.GE25291
HARVESTED ON: 6/3/2008
SEQUENCED ON: 3/16/2012
EXPECTED MW: 57kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI LKLYNTRTKD FSELTNFENV KVYACGPTVY NYAHIGNFRT YIFGDLLIKT LRFLGYKVNY AMNITDIGHL TGDLDDGEDK VAKTAREKGL TVYEISEFFT EAFFNDCRKL NIVYPDKVLV ASKHIPIMIE VVKILEEKKI TYFSNGNVYF DTSCFKSYGE MAGIDLIDKD MTLPRVDVDK FKRNKTDFVL WFTNSKFKDQ EMKWDSPWGF GYPSWHLECA AMNLEYFKDA LDIHLGGVDH IGVHHINEIA IAECFLNKKW CDVFVHGEFL IMDYNKMSKS RGNFITVKDL EDQNFSPLDF RYLCLTSHYR NQLKFSLDNL QASKIARENL INKLSYFYES LDPVDLNTLN KDLKNFGFSV EKEYYDSFVE KISFDLNVAQ GLALLWEIIK SDNLSFVSKL RLAFIFDEIM SLNLREEILK NLQNHDVVID ENMKALIEER RIAKCEKNFK RADEIRDFFA KKGFVLVDTK EGTKVKRG
Validated NT Sequence
ttaagaagga gatataccat ggctcaccac caccaccacc atatgatttt aaagttatat aatactagaa caaaggattt ttcagaatta acaaattttg aaaatgttaa agtgtatgct tgcgggccta ctgtttataa ttatgctcac atcgggaatt ttagaactta tatttttgga gatttgttaa ttaaaacttt aaggtttttg gggtataaag ttaattatgc gatgaatatt acagatattg gacatttaac aggtgatctt gatgatggag aagataaggt tgctaagacc gcaagagaga agggccttac agtttatgag attagtgaat ttttcacaga ggcttttttt aacgattgta gaaaattaaa cattgtatat cccgacaaag ttcttgttgc aagtaaacat attcccataa tgatagaggt tgttaaaatt cttgaagaaa aaaaaattac ttatttttct aatggtaatg tgtattttga tacttcttgt tttaaaagct atggtgagat ggccggcatt gatcttattg ataaagatat gactttaccc agagttgatg ttgataaatt taaaaggaat aaaaccgatt ttgttttgtg gtttactaat tctaagttta aagatcagga gatgaaatgg gattctcctt ggggatttgg ttatccaagt tggcatttgg agtgcgctgc gatgaatttg gagtatttca aagatgctct tgatattcat ttgggaggag ttgatcatat tggagttcac cacataaatg aaatagcaat agcagagtgt tttttgaata aaaaatggtg tgatgtcttt gttcatggag aatttttgat tatggattat aataagatgt caaagtcacg tggaaatttt attacagtta aagatttaga agatcaaaat ttttcccctc ttgattttag atatttatgt ttgacatcgc actacaggaa ccaattaaaa ttttcattag ataatcttca agcaagcaag attgctagag aaaatttgat aaacaagcta agttattttt atgaatcttt agatccagtt gatttaaata cacttaataa ggatttaaaa aattttggtt ttagtgtaga aaaagaatat tatgactctt ttgtagaaaa aatttctttt gatttaaatg ttgctcaagg attggctttg ctttgggaga taattaaatc tgacaatcta agctttgttt caaagcttag attagctttt atttttgatg agattatgtc acttaatctg agagaagaaa ttttaaaaaa tttacaaaac catgacgtag ttattgatga gaatatgaaa gctttgattg aagagagaag aatagctaaa tgtgaaaaaa attttaagcg tgctgatgaa atcagagatt tttttgccaa aaaaggtttt gttttggttg atactaagga aggaactaag gttaaaagag gctaagtgag taagat
Expected Protein Sequence
MAHHHHHHMI LKLYNTRTKD FSELTNFENV KVYACGPTVY NYAHIGNFRT YIFGDLLIKT LRFLGYKVNY AMNITDIGHL TGDLDDGEDK VAKTAREKGL TVYEISEFFT EAFFNDCRKL NIVYPDKVLV ASKHIPIMIE VVKILEEKKI TYFSNGNVYF DTSCFKSYGE MAGIDLIDKD MTLPRVDVDK FKRNKTDFVL WFTNSKFKDQ EMKWDSPWGF GYPSWHLECA AMNLEYFKDA LDIHLGGVDH IGVHHINEIA IAECFLNKKW CDVFVHGEFL IMDYNKMSKS RGNFITVKDL EDQNFSPLDF RYLCLTSHYR NQLKFSLDNL QASKIARENL INKLSYFYES LDPVDLNTLN KDLKNFGFSV EKEYYDSFVE KISFDLNVAQ GLALLWEIIK SDNLSFVSKL RLAFIFDEIM SLNLREEILK NLQNHDVVID ENMKALIEER RIAKCEKNFK RADEIRDFFA KKGFVLVDTK EGTKVKRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattt taaagttata taatactaga acaaaggatt tttcagaatt aacaaatttt gaaaatgtta aagtgtatgc ttgcgggcct actgtttata attatgctca catcgggaat tttagaactt atatttttgg agatttgtta attaaaactt taaggttttt ggggtataaa gttaattatg cgatgaatat tacagatatt ggacatttaa caggtgatct tgatgatgga gaagataagg ttgctaagac cgcaagagag aagggcctta cagtttatga gattagtgaa tttttcacag aggctttttt taacgattgt agaaaattaa acattgtata tcccgacaaa gttcttgttg caagtaaaca tattcccata atgatagagg ttgttaaaat tcttgaagaa aaaaaaatta cttatttttc taatggtaat gtgtattttg atacttcttg ttttaaaagc tatggtgaga tggccggcat tgatcttatt gataaagata tgactttacc cagagttgat gttgataaat ttaaaaggaa taaaaccgat tttgttttgt ggtttactaa ttctaagttt aaagatcagg agatgaaatg ggattctcct tggggatttg gttatccaag ttggcatttg gagtgcgctg cgatgaattt ggagtatttc aaagatgctc ttgatattca tttgggagga gttgatcata ttggagttca ccacataaat gaaatagcaa tagcagagtg ttttttgaat aaaaaatggt gtgatgtctt tgttcatgga gaatttttga ttatggatta taataagatg tcaaagtcac gtggaaattt tattacagtt aaagatttag aagatcaaaa tttttcccct cttgatttta gatatttatg tttgacatcg cactacagga accaattaaa attttcatta gataatcttc aagcaagcaa gattgctaga gaaaatttga taaacaagct aagttatttt tatgaatctt tagatccagt tgatttaaat acacttaata aggatttaaa aaattttggt tttagtgtag aaaaagaata ttatgactct tttgtagaaa aaatttcttt tgatttaaat gttgctcaag gattggcttt gctttgggag ataattaaat ctgacaatct aagctttgtt tcaaagctta gattagcttt tatttttgat gagattatgt cacttaatct gagagaagaa attttaaaaa atttacaaaa ccatgacgta gttattgatg agaatatgaa agctttgatt gaagagagaa gaatagctaa atgtgaaaaa aattttaagc gtgctgatga aatcagagat ttttttgcca aaaaaggttt tgttttggtt gatactaagg aaggaactaa ggttaaaaga ggctaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BobuA.00133.a.B1.PS00097
PURIFICATION DATe: 10/5/2008
CONCENTRATION: 17.94mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI LKLYNTRTKD FSELTNFENV KVYACGPTVY NYAHIGNFRT YIFGDLLIKT LRFLGYKVNY AMNITDIGHL TGDLDDGEDK VAKTAREKGL TVYEISEFFT EAFFNDCRKL NIVYPDKVLV ASKHIPIMIE VVKILEEKKI TYFSNGNVYF DTSCFKSYGE MAGIDLIDKD MTLPRVDVDK FKRNKTDFVL WFTNSKFKDQ EMKWDSPWGF GYPSWHLECA AMNLEYFKDA LDIHLGGVDH IGVHHINEIA IAECFLNKKW CDVFVHGEFL IMDYNKMSKS RGNFITVKDL EDQNFSPLDF RYLCLTSHYR NQLKFSLDNL QASKIARENL INKLSYFYES LDPVDLNTLN KDLKNFGFSV EKEYYDSFVE KISFDLNVAQ GLALLWEIIK SDNLSFVSKL RLAFIFDEIM SLNLREEILK NLQNHDVVID ENMKALIEER RIAKCEKNFK RADEIRDFFA KKGFVLVDTK EGTKVKRG
Validated NT Sequence
ttaagaagga gatataccat ggctcaccac caccaccacc atatgatttt aaagttatat aatactagaa caaaggattt ttcagaatta acaaattttg aaaatgttaa agtgtatgct tgcgggccta ctgtttataa ttatgctcac atcgggaatt ttagaactta tatttttgga gatttgttaa ttaaaacttt aaggtttttg gggtataaag ttaattatgc gatgaatatt acagatattg gacatttaac aggtgatctt gatgatggag aagataaggt tgctaagacc gcaagagaga agggccttac agtttatgag attagtgaat ttttcacaga ggcttttttt aacgattgta gaaaattaaa cattgtatat cccgacaaag ttcttgttgc aagtaaacat attcccataa tgatagaggt tgttaaaatt cttgaagaaa aaaaaattac ttatttttct aatggtaatg tgtattttga tacttcttgt tttaaaagct atggtgagat ggccggcatt gatcttattg ataaagatat gactttaccc agagttgatg ttgataaatt taaaaggaat aaaaccgatt ttgttttgtg gtttactaat tctaagttta aagatcagga gatgaaatgg gattctcctt ggggatttgg ttatccaagt tggcatttgg agtgcgctgc gatgaatttg gagtatttca aagatgctct tgatattcat ttgggaggag ttgatcatat tggagttcac cacataaatg aaatagcaat agcagagtgt tttttgaata aaaaatggtg tgatgtcttt gttcatggag aatttttgat tatggattat aataagatgt caaagtcacg tggaaatttt attacagtta aagatttaga agatcaaaat ttttcccctc ttgattttag atatttatgt ttgacatcgc actacaggaa ccaattaaaa ttttcattag ataatcttca agcaagcaag attgctagag aaaatttgat aaacaagcta agttattttt atgaatcttt agatccagtt gatttaaata cacttaataa ggatttaaaa aattttggtt ttagtgtaga aaaagaatat tatgactctt ttgtagaaaa aatttctttt gatttaaatg ttgctcaagg attggctttg ctttgggaga taattaaatc tgacaatcta agctttgttt caaagcttag attagctttt atttttgatg agattatgtc acttaatctg agagaagaaa ttttaaaaaa tttacaaaac catgacgtag ttattgatga gaatatgaaa gctttgattg aagagagaag aatagctaaa tgtgaaaaaa attttaagcg tgctgatgaa atcagagatt tttttgccaa aaaaggtttt gttttggttg atactaagga aggaactaag gttaaaagag gctaagtgag taagat
Expressed Protein Sequence
MAHHHHHHMI LKLYNTRTKD FSELTNFENV KVYACGPTVY NYAHIGNFRT YIFGDLLIKT LRFLGYKVNY AMNITDIGHL TGDLDDGEDK VAKTAREKGL TVYEISEFFT EAFFNDCRKL NIVYPDKVLV ASKHIPIMIE VVKILEEKKI TYFSNGNVYF DTSCFKSYGE MAGIDLIDKD MTLPRVDVDK FKRNKTDFVL WFTNSKFKDQ EMKWDSPWGF GYPSWHLECA AMNLEYFKDA LDIHLGGVDH IGVHHINEIA IAECFLNKKW CDVFVHGEFL IMDYNKMSKS RGNFITVKDL EDQNFSPLDF RYLCLTSHYR NQLKFSLDNL QASKIARENL INKLSYFYES LDPVDLNTLN KDLKNFGFSV EKEYYDSFVE KISFDLNVAQ GLALLWEIIK SDNLSFVSKL RLAFIFDEIM SLNLREEILK NLQNHDVVID ENMKALIEER RIAKCEKNFK RADEIRDFFA KKGFVLVDTK EGTKVKRG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTT TAAAGTTATA TAATACTAGA ACAAAGGATT TTTCAGAATT AACAAATTTT GAAAATGTTA AAGTGTATGC TTGCGGGCCT ACTGTTTATA ATTATGCTCA CATCGGGAAT TTTAGAACTT ATATTTTTGG AGATTTGTTA ATTAAAACTT TAAGGTTTTT GGGGTATAAA GTTAATTATG CGATGAATAT TACAGATATT GGACATTTAA CAGGTGATCT TGATGATGGA GAAGATAAGG TTGCTAAGAC CGCAAGAGAG AAGGGCCTTA CAGTTTATGA GATTAGTGAA TTTTTCACAG AGGCTTTTTT TAACGATTGT AGAAAATTAA ACATTGTATA TCCCGACAAA GTTCTTGTTG CAAGTAAACA TATTCCCATA ATGATAGAGG TTGTTAAAAT TCTTGAAGAA AAAAAAATTA CTTATTTTTC TAATGGTAAT GTGTATTTTG ATACTTCTTG TTTTAAAAGC TATGGTGAGA TGGCCGGCAT TGATCTTATT GATAAAGATA TGACTTTACC CAGAGTTGAT GTTGATAAAT TTAAAAGGAA TAAAACCGAT TTTGTTTTGT GGTTTACTAA TTCTAAGTTT AAAGATCAGG AGATGAAATG GGATTCTCCT TGGGGATTTG GTTATCCAAG TTGGCATTTG GAGTGCGCTG CGATGAATTT GGAGTATTTC AAAGATGCTC TTGATATTCA TTTGGGAGGA GTTGATCATA TTGGAGTTCA CCACATAAAT GAAATAGCAA TAGCAGAGTG TTTTTTGAAT AAAAAATGGT GTGATGTCTT TGTTCATGGA GAATTTTTGA TTATGGATTA TAATAAGATG TCAAAGTCAC GTGGAAATTT TATTACAGTT AAAGATTTAG AAGATCAAAA TTTTTCCCCT CTTGATTTTA GATATTTATG TTTGACATCG CACTACAGGA ACCAATTAAA ATTTTCATTA GATAATCTTC AAGCAAGCAA GATTGCTAGA GAAAATTTGA TAAACAAGCT AAGTTATTTT TATGAATCTT TAGATCCAGT TGATTTAAAT ACACTTAATA AGGATTTAAA AAATTTTGGT TTTAGTGTAG AAAAAGAATA TTATGACTCT TTTGTAGAAA AAATTTCTTT TGATTTAAAT GTTGCTCAAG GATTGGCTTT GCTTTGGGAG ATAATTAAAT CTGACAATCT AAGCTTTGTT TCAAAGCTTA GATTAGCTTT TATTTTTGAT GAGATTATGT CACTTAATCT GAGAGAAGAA ATTTTAAAAA ATTTACAAAA CCATGACGTA GTTATTGATG AGAATATGAA AGCTTTGATT GAAGAGAGAA GAATAGCTAA ATGTGAAAAA AATTTTAAGC GTGCTGATGA AATCAGAGAT TTTTTTGCCA AAAAAGGTTT TGTTTTGGTT GATACTAAGG AAGGAACTAA GGTTAAAAGA GGCTAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for BobuA.00133.a.A1.PW25233
PURIFICATION DATe: 5/30/2008
CONCENTRATION: 26.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMILKLYNTR TKDFSELTNF ENVKVYACGP TVYNYAHIGN FRTYIFGDLL IKTLRFLGYK VNYAMNITDI GHLTGDLDDG EDKVAKTARE KGLTVYEISE FFTEAFFNDC RKLNIVYPDK VLVASKHIPI MIEVVKILEE KKITYFSNGN VYFDTSCFKS YGEMAGIDLI DKDMTLPRVD VDKFKRNKTD FVLWFTNSKF KDQEMKWDSP WGFGYPSWHL ECAAMNLEYF KDALDIHLGG VDHIGVHHIN EIAIAECFLN KKWCDVFVHG EFLIMDYNKM SKSRGNFITV KDLEDQNFSP LDFRYLCLTS HYRNQLKFSL DNLQASKIAR ENLINKLSYF YESLDPVDLN TLNKDLKNFG FSVEKEYYDS FVEKISFDLN VAQGLALLWE IIKSDNLSFV SKLRLAFIFD EIMSLNLREE ILKNLQNHDV VIDENMKALI EERRIAKCEK NFKRADEIRD FFAKKGFVLV DTKEGTKVKR G
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatttt aaagttatat aatactagaa caaaggattt ttcagaatta acaaattttg aaaatgttaa agtgtatgct tgcgggccta ctgtttataa ttatgctcac atcgggaatt ttagaactta tatttttgga gatttgttaa ttaaaacttt aaggtttttg gggtataaag ttaattatgc gatgaatatt acagatattg gacatttaac aggtgatctt gatgatggag aagataaggt tgctaagacc gcaagagaga agggccttac agtttatgag attagtgaat ttttcacaga ggcttttttt aacgattgta gaaaattaaa cattgtatat cccgacaaag ttcttgttgc aagtaaacat attcccataa tgatagaggt tgttaaaatt cttgaagaaa aaaaaattac ttatttttct aatggtaatg tgtattttga tacttcttgt tttaaaagct atggtgagat ggccggcatt gatcttattg ataaagatat gactttaccc agagttgatg ttgataaatt taaaaggaat aaaaccgatt ttgttttgtg gtttactaat tctaagttta aagatcagga gatgaaatgg gattctcctt ggggatttgg ttatccaagt tggcatttgg agtgcgctgc gatgaatttg gagtatttca aagatgctct tgatattcat ttgggaggag ttgatcatat tggagttcac cacataaatg aaatagcaat agcagagtgt tttttgaata aaaaatggtg tgatgtcttt gttcatggag aatttttgat tatggattat aataagatgt caaagtcacg tggaaatttt attacagtta aagatttaga agatcaaaat ttttcccctc ttgattttag atatttatgt ttgacatcgc actacaggaa ccaattaaaa ttttcattag ataatcttca agcaagcaag attgctagag aaaatttgat aaacaagcta agttattttt atgaatcttt agatccagtt gatttaaata cacttaataa ggatttaaaa aattttggtt ttagtgtaga aaaagaatat tatgactctt ttgtagaaaa aatttctttt gatttaaatg ttgctcaagg attggctttg ctttgggaga taattaaatc tgacaatcta agctttgttt caaagcttag attagctttt atttttgatg agattatgtc acttaatctg agagaagaaa ttttaaaaaa tttacaaaac catgacgtag ttattgatga gaatatgaaa gctttgattg aagagagaag aatagctaaa tgtgaaaaaa attttaagcg tgctgatgaa atcagagatt tttttgccaa aaaaggtttt gttttggttg atactaagga aggaactaag gttaaaagag gctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILKLYNTR TKDFSELTNF ENVKVYACGP TVYNYAHIGN FRTYIFGDLL IKTLRFLGYK VNYAMNITDI GHLTGDLDDG EDKVAKTARE KGLTVYEISE FFTEAFFNDC RKLNIVYPDK VLVASKHIPI MIEVVKILEE KKITYFSNGN VYFDTSCFKS YGEMAGIDLI DKDMTLPRVD VDKFKRNKTD FVLWFTNSKF KDQEMKWDSP WGFGYPSWHL ECAAMNLEYF KDALDIHLGG VDHIGVHHIN EIAIAECFLN KKWCDVFVHG EFLIMDYNKM SKSRGNFITV KDLEDQNFSP LDFRYLCLTS HYRNQLKFSL DNLQASKIAR ENLINKLSYF YESLDPVDLN TLNKDLKNFG FSVEKEYYDS FVEKISFDLN VAQGLALLWE IIKSDNLSFV SKLRLAFIFD EIMSLNLREE ILKNLQNHDV VIDENMKALI EERRIAKCEK NFKRADEIRD FFAKKGFVLV DTKEGTKVKR G
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTTTAAAG TTATATAATA CTAGAACAAA GGATTTTTCA GAATTAACAA ATTTTGAAAA TGTTAAAGTG TATGCTTGCG GGCCTACTGT TTATAATTAT GCTCACATCG GGAATTTTAG AACTTATATT TTTGGAGATT TGTTAATTAA AACTTTAAGG TTTTTGGGGT ATAAAGTTAA TTATGCGATG AATATTACAG ATATTGGACA TTTAACAGGT GATCTTGATG ATGGAGAAGA TAAGGTTGCT AAGACCGCAA GAGAGAAGGG CCTTACAGTT TATGAGATTA GTGAATTTTT CACAGAGGCT TTTTTTAACG ATTGTAGAAA ATTAAACATT GTATATCCCG ACAAAGTTCT TGTTGCAAGT AAACATATTC CCATAATGAT AGAGGTTGTT AAAATTCTTG AAGAAAAAAA AATTACTTAT TTTTCTAATG GTAATGTGTA TTTTGATACT TCTTGTTTTA AAAGCTATGG TGAGATGGCC GGCATTGATC TTATTGATAA AGATATGACT TTACCCAGAG TTGATGTTGA TAAATTTAAA AGGAATAAAA CCGATTTTGT TTTGTGGTTT ACTAATTCTA AGTTTAAAGA TCAGGAGATG AAATGGGATT CTCCTTGGGG ATTTGGTTAT CCAAGTTGGC ATTTGGAGTG CGCTGCGATG AATTTGGAGT ATTTCAAAGA TGCTCTTGAT ATTCATTTGG GAGGAGTTGA TCATATTGGA GTTCACCACA TAAATGAAAT AGCAATAGCA GAGTGTTTTT TGAATAAAAA ATGGTGTGAT GTCTTTGTTC ATGGAGAATT TTTGATTATG GATTATAATA AGATGTCAAA GTCACGTGGA AATTTTATTA CAGTTAAAGA TTTAGAAGAT CAAAATTTTT CCCCTCTTGA TTTTAGATAT TTATGTTTGA CATCGCACTA CAGGAACCAA TTAAAATTTT CATTAGATAA TCTTCAAGCA AGCAAGATTG CTAGAGAAAA TTTGATAAAC AAGCTAAGTT ATTTTTATGA ATCTTTAGAT CCAGTTGATT TAAATACACT TAATAAGGAT TTAAAAAATT TTGGTTTTAG TGTAGAAAAA GAATATTATG ACTCTTTTGT AGAAAAAATT TCTTTTGATT TAAATGTTGC TCAAGGATTG GCTTTGCTTT GGGAGATAAT TAAATCTGAC AATCTAAGCT TTGTTTCAAA GCTTAGATTA GCTTTTATTT TTGATGAGAT TATGTCACTT AATCTGAGAG AAGAAATTTT AAAAAATTTA CAAAACCATG ACGTAGTTAT TGATGAGAAT ATGAAAGCTT TGATTGAAGA GAGAAGAATA GCTAAATGTG AAAAAAATTT TAAGCGTGCT GATGAAATCA GAGATTTTTT TGCCAAAAAA GGTTTTGTTT TGGTTGATAC TAAGGAAGGA ACTAAGGTTA AAAGAGGCTA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for BobuA.00133.a.A1.PW27585
PURIFICATION DATe: 9/22/2009
CONCENTRATION: 25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMILKLYNTR TKDFSELTNF ENVKVYACGP TVYNYAHIGN FRTYIFGDLL IKTLRFLGYK VNYAMNITDI GHLTGDLDDG EDKVAKTARE KGLTVYEISE FFTEAFFNDC RKLNIVYPDK VLVASKHIPI MIEVVKILEE KKITYFSNGN VYFDTSCFKS YGEMAGIDLI DKDMTLPRVD VDKFKRNKTD FVLWFTNSKF KDQEMKWDSP WGFGYPSWHL ECAAMNLEYF KDALDIHLGG VDHIGVHHIN EIAIAECFLN KKWCDVFVHG EFLIMDYNKM SKSRGNFITV KDLEDQNFSP LDFRYLCLTS HYRNQLKFSL DNLQASKIAR ENLINKLSYF YESLDPVDLN TLNKDLKNFG FSVEKEYYDS FVEKISFDLN VAQGLALLWE IIKSDNLSFV SKLRLAFIFD EIMSLNLREE ILKNLQNHDV VIDENMKALI EERRIAKCEK NFKRADEIRD FFAKKGFVLV DTKEGTKVKR G
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatttt aaagttatat aatactagaa caaaggattt ttcagaatta acaaattttg aaaatgttaa agtgtatgct tgcgggccta ctgtttataa ttatgctcac atcgggaatt ttagaactta tatttttgga gatttgttaa ttaaaacttt aaggtttttg gggtataaag ttaattatgc gatgaatatt acagatattg gacatttaac aggtgatctt gatgatggag aagataaggt tgctaagacc gcaagagaga agggccttac agtttatgag attagtgaat ttttcacaga ggcttttttt aacgattgta gaaaattaaa cattgtatat cccgacaaag ttcttgttgc aagtaaacat attcccataa tgatagaggt tgttaaaatt cttgaagaaa aaaaaattac ttatttttct aatggtaatg tgtattttga tacttcttgt tttaaaagct atggtgagat ggccggcatt gatcttattg ataaagatat gactttaccc agagttgatg ttgataaatt taaaaggaat aaaaccgatt ttgttttgtg gtttactaat tctaagttta aagatcagga gatgaaatgg gattctcctt ggggatttgg ttatccaagt tggcatttgg agtgcgctgc gatgaatttg gagtatttca aagatgctct tgatattcat ttgggaggag ttgatcatat tggagttcac cacataaatg aaatagcaat agcagagtgt tttttgaata aaaaatggtg tgatgtcttt gttcatggag aatttttgat tatggattat aataagatgt caaagtcacg tggaaatttt attacagtta aagatttaga agatcaaaat ttttcccctc ttgattttag atatttatgt ttgacatcgc actacaggaa ccaattaaaa ttttcattag ataatcttca agcaagcaag attgctagag aaaatttgat aaacaagcta agttattttt atgaatcttt agatccagtt gatttaaata cacttaataa ggatttaaaa aattttggtt ttagtgtaga aaaagaatat tatgactctt ttgtagaaaa aatttctttt gatttaaatg ttgctcaagg attggctttg ctttgggaga taattaaatc tgacaatcta agctttgttt caaagcttag attagctttt atttttgatg agattatgtc acttaatctg agagaagaaa ttttaaaaaa tttacaaaac catgacgtag ttattgatga gaatatgaaa gctttgattg aagagagaag aatagctaaa tgtgaaaaaa attttaagcg tgctgatgaa atcagagatt tttttgccaa aaaaggtttt gttttggttg atactaagga aggaactaag gttaaaagag gctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILKLYNTR TKDFSELTNF ENVKVYACGP TVYNYAHIGN FRTYIFGDLL IKTLRFLGYK VNYAMNITDI GHLTGDLDDG EDKVAKTARE KGLTVYEISE FFTEAFFNDC RKLNIVYPDK VLVASKHIPI MIEVVKILEE KKITYFSNGN VYFDTSCFKS YGEMAGIDLI DKDMTLPRVD VDKFKRNKTD FVLWFTNSKF KDQEMKWDSP WGFGYPSWHL ECAAMNLEYF KDALDIHLGG VDHIGVHHIN EIAIAECFLN KKWCDVFVHG EFLIMDYNKM SKSRGNFITV KDLEDQNFSP LDFRYLCLTS HYRNQLKFSL DNLQASKIAR ENLINKLSYF YESLDPVDLN TLNKDLKNFG FSVEKEYYDS FVEKISFDLN VAQGLALLWE IIKSDNLSFV SKLRLAFIFD EIMSLNLREE ILKNLQNHDV VIDENMKALI EERRIAKCEK NFKRADEIRD FFAKKGFVLV DTKEGTKVKR G
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTTTAAAG TTATATAATA CTAGAACAAA GGATTTTTCA GAATTAACAA ATTTTGAAAA TGTTAAAGTG TATGCTTGCG GGCCTACTGT TTATAATTAT GCTCACATCG GGAATTTTAG AACTTATATT TTTGGAGATT TGTTAATTAA AACTTTAAGG TTTTTGGGGT ATAAAGTTAA TTATGCGATG AATATTACAG ATATTGGACA TTTAACAGGT GATCTTGATG ATGGAGAAGA TAAGGTTGCT AAGACCGCAA GAGAGAAGGG CCTTACAGTT TATGAGATTA GTGAATTTTT CACAGAGGCT TTTTTTAACG ATTGTAGAAA ATTAAACATT GTATATCCCG ACAAAGTTCT TGTTGCAAGT AAACATATTC CCATAATGAT AGAGGTTGTT AAAATTCTTG AAGAAAAAAA AATTACTTAT TTTTCTAATG GTAATGTGTA TTTTGATACT TCTTGTTTTA AAAGCTATGG TGAGATGGCC GGCATTGATC TTATTGATAA AGATATGACT TTACCCAGAG TTGATGTTGA TAAATTTAAA AGGAATAAAA CCGATTTTGT TTTGTGGTTT ACTAATTCTA AGTTTAAAGA TCAGGAGATG AAATGGGATT CTCCTTGGGG ATTTGGTTAT CCAAGTTGGC ATTTGGAGTG CGCTGCGATG AATTTGGAGT ATTTCAAAGA TGCTCTTGAT ATTCATTTGG GAGGAGTTGA TCATATTGGA GTTCACCACA TAAATGAAAT AGCAATAGCA GAGTGTTTTT TGAATAAAAA ATGGTGTGAT GTCTTTGTTC ATGGAGAATT TTTGATTATG GATTATAATA AGATGTCAAA GTCACGTGGA AATTTTATTA CAGTTAAAGA TTTAGAAGAT CAAAATTTTT CCCCTCTTGA TTTTAGATAT TTATGTTTGA CATCGCACTA CAGGAACCAA TTAAAATTTT CATTAGATAA TCTTCAAGCA AGCAAGATTG CTAGAGAAAA TTTGATAAAC AAGCTAAGTT ATTTTTATGA ATCTTTAGAT CCAGTTGATT TAAATACACT TAATAAGGAT TTAAAAAATT TTGGTTTTAG TGTAGAAAAA GAATATTATG ACTCTTTTGT AGAAAAAATT TCTTTTGATT TAAATGTTGC TCAAGGATTG GCTTTGCTTT GGGAGATAAT TAAATCTGAC AATCTAAGCT TTGTTTCAAA GCTTAGATTA GCTTTTATTT TTGATGAGAT TATGTCACTT AATCTGAGAG AAGAAATTTT AAAAAATTTA CAAAACCATG ACGTAGTTAT TGATGAGAAT ATGAAAGCTT TGATTGAAGA GAGAAGAATA GCTAAATGTG AAAAAAATTT TAAGCGTGCT GATGAAATCA GAGATTTTTT TGCCAAAAAA GGTTTTGTTT TGGTTGATAC TAAGGAAGGA ACTAAGGTTA AAAGAGGCTA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA