BobuA.00085.a

nucleotide sugar epimerase (BB_0444)

CENTER ID: BobuA.00085.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.00085.a.B2.GE39050 soluble domain 19 355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.835
RefSeq: NP_212578.1
UniProt: O51400

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIFLTGIAG FIGFHVAKKL VEKGHEVLGI DVLNDYYELK FKHERLEALG FCSKDVKTHK IIKSEKYNNL SFAYLDILNK DKLLELFKDH KFTHVCHLAA QAGIRDSLEN PDSYVSINIV GFFNVLDVCR VYKENIEHFV YASTSSVYGI NENMPSSEDS ITDHPLNLYA ASKKSNEMMA HAYSASFNIP TTGLRFFTVY GTYGRPDMAL YLFSDGIKNG KAINIFNNGN MARDFTYVGD IADGVYKVLK NPAKSDCNFD VKNPNSSTSS FPYRIYNIGT GHATKLLDFI SELEANFDKK ALKNYMPMQK ADVVESCCDI LKLKNDVGYE AKVSIKEGIK EFSQWYKMLE STKKT
NT Sequence
atgaagattt ttttaaccgg aattgcaggg tttattggat ttcatgtagc taaaaagctt gtggaaaaag ggcatgaagt tttgggaata gacgtattaa atgattatta tgaactcaaa tttaagcatg aaaggttaga agctttaggg ttttgttcta aggatgttaa aacgcataag attattaaga gtgaaaaata taataattta tcttttgctt atcttgatat actaaataag gataaactgc tagaactttt taaagatcat aagtttacac atgtttgcca tttggctgct caagcgggca ttagagatag tcttgaaaat cctgatagct atgtttcaat aaatattgtt gggtttttta atgttttgga tgtatgtaga gtttataaag aaaacatcga gcattttgtt tatgcatcaa cgtcatcggt ttatggcata aatgaaaata tgccgtctag tgaagattct attacggatc accctttaaa tttatatgca gctagtaaaa aatctaatga gatgatggcg catgcttata gtgcatcttt taatattccc acaacagggc ttagattttt cacagtttat gggacttatg gaagacccga tatggcttta tatttatttt cagatggaat taaaaatggc aaggctatta atatttttaa taatgggaat atggctagag attttacata tgtgggtgac attgcagacg gtgtttacaa agttttgaaa aatccggcta agagtgattg taattttgac gttaaaaatc caaattcatc aacttcttct tttccttaca gaatatataa tataggaact ggacacgcaa ctaaactgct agattttatt agcgagcttg aagcaaattt tgataaaaag gccttaaaaa attatatgcc tatgcaaaaa gcagatgttg tagaaagttg ttgtgatatt ttaaagctta aaaatgatgt tgggtatgag gctaaagttt ctattaaaga aggaataaaa gaattttcac agtggtataa aatgctagag tctaccaaga agacttga
Details for BobuA.00085.a.B2.GE39050
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM KLVEKGHEVL GIDVLNDYYE LKFKHERLEA LGFCSKDVKT HKIIKSEKYN NLSFAYLDIL NKDKLLELFK DHKFTHVCHL AAQAGIRDSL ENPDSYVSIN IVGFFNVLDV CRVYKENIEH FVYASTSSVY GINENMPSSE DSITDHPLNL YAASKKSNEM MAHAYSASFN IPTTGLRFFT VYGTYGRPDM ALYLFSDGIK NGKAINIFNN GNMARDFTYV GDIADGVYKV LKNPAKSDCN FDVKNPNSST SSFPYRIYNI GTGHATKLLD FISELEANFD KKALKNYMPM QKADVVESCC DILKLKNDVG YEAKVSIKEG IKEFSQWYKM LESTKKT
Validated NT Sequence
tcacttaagt cttcttggta gactctagca ttttatacca ctgtgaaaat tcttttattc cttctttaat agaaacttta gcctcatacc caacatcatt tttaagcttt aaaatatcac aacaactttc tacaacatct gctttttgca taggcatata attttttaag gcctttttat caaaatttgc ttcaagctcg ctaataaaat ctagcagttt agttgcgtgt ccagttccta tattatatat tctgtaagga aaagaagaag ttgatgaatt tggattttta acgtcaaaat tacaatcact cttagccgga tttttcaaaa ctttgtaaac accgtctgca atgtcaccca catatgtaaa atctctagcc atattcccat tattaaaaat attaatagcc ttgccatttt taattccatc tgaaaataaa tataaagcca tatcgggtct tccataagtc ccataaactg tgaaaaatct aagccctgtt gtgggaatat taaaagatgc actataagca tgcgccatca tctcattaga ttttttacta gctgcatata aatttaaagg gtgatccgta atagaatctt cactagacgg catattttca tttatgccat aaaccgatga cgttgatgca taaacaaaat gctcgatgtt ttctttataa actctacata catccaaaac attaaaaaac ccaacaatat ttattgaaac atagctatca ggattttcaa gactatctct aatgcccgct tgagcagcca aatggcaaac atgtgtaaac ttatgatctt taaaaagttc tagcagttta tccttattta gtatatcaag ataagcaaaa gataaattat tatatttttc actcttaata atcttatgcg ttttaacatc cttagaacaa aaccctaaag cttctaacct ttcatgctta aatttgagtt cataataatc atttaatacg tctattccca aaacttcatg ccctttttcc acaagcttca tatggtggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHKL VEKGHEVLGI DVLNDYYELK FKHERLEALG FCSKDVKTHK IIKSEKYNNL SFAYLDILNK DKLLELFKDH KFTHVCHLAA QAGIRDSLEN PDSYVSINIV GFFNVLDVCR VYKENIEHFV YASTSSVYGI NENMPSSEDS ITDHPLNLYA ASKKSNEMMA HAYSASFNIP TTGLRFFTVY GTYGRPDMAL YLFSDGIKNG KAINIFNNGN MARDFTYVGD IADGVYKVLK NPAKSDCNFD VKNPNSSTSS FPYRIYNIGT GHATKLLDFI SELEANFDKK ALKNYMPMQK ADVVESCCDI LKLKNDVGYE AKVSIKEGIK EFSQWYKMLE STKKT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagcttg tggaaaaagg gcatgaagtt ttgggaatag acgtattaaa tgattattat gaactcaaat ttaagcatga aaggttagaa gctttagggt tttgttctaa ggatgttaaa acgcataaga ttattaagag tgaaaaatat aataatttat cttttgctta tcttgatata ctaaataagg ataaactgct agaacttttt aaagatcata agtttacaca tgtttgccat ttggctgctc aagcgggcat tagagatagt cttgaaaatc ctgatagcta tgtttcaata aatattgttg ggttttttaa tgttttggat gtatgtagag tttataaaga aaacatcgag cattttgttt atgcatcaac gtcatcggtt tatggcataa atgaaaatat gccgtctagt gaagattcta ttacggatca ccctttaaat ttatatgcag ctagtaaaaa atctaatgag atgatggcgc atgcttatag tgcatctttt aatattccca caacagggct tagatttttc acagtttatg ggacttatgg aagacccgat atggctttat atttattttc agatggaatt aaaaatggca aggctattaa tatttttaat aatgggaata tggctagaga ttttacatat gtgggtgaca ttgcagacgg tgtttacaaa gttttgaaaa atccggctaa gagtgattgt aattttgacg ttaaaaatcc aaattcatca acttcttctt ttccttacag aatatataat ataggaactg gacacgcaac taaactgcta gattttatta gcgagcttga agcaaatttt gataaaaagg ccttaaaaaa ttatatgcct atgcaaaaag cagatgttgt agaaagttgt tgtgatattt taaagcttaa aaatgatgtt gggtatgagg ctaaagtttc tattaaagaa ggaataaaag aattttcaca gtggtataaa atgctagagt ctaccaagaa gacttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat