BewuA.18928.a

orf1ab polyprotein

CENTER ID: BewuA.18928.a
ORGANISM: Betacoronavirus SARS-CoV-2 (2019-nCoV) (COVID-19) isolate Wuhan-Hu-1
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BewuA.18928.a.MX101.PB00110 nsp9 4141 4253
BewuA.18928.a.MX81.PB00111 nsp7 3860 3942
BewuA.18928.a.R91.PB00112 nsp8 3943 4140
BewuA.18928.a.MX81.PS38718 nsp7 3860 3942
BewuA.18928.a.MX111.PS38719 nsp10 4254 4392
BewuA.18928.a.R91.PW38804 nsp8 3943 4140
BewuA.18928.a.MX101.PW38805 nsp9 4141 4253
BewuA.18928.a.MX161.PW38807 nsp16 (2prime-O-methyltransferase) 6799 7096
BewuA.18928.a.MX111.PW38819 nsp10 4254 4392
BewuA.18928.a.A142.PW38844 ExoN (Guanine-N7 methyltransferase) (nsp14) 6212 6452

Structures

6XDH
DEPOSITED: 6/10/2020
DETERMINATION: XRay
CLONE: BewuA.18928.a.MX151.GE43650
PROTEIN: BewuA.18928.a.MX151.PW38806
8U2X
DEPOSITED: 9/6/2023
DETERMINATION: XRay
CLONE: BewuA.18928.a.MX152.GE44774
PROTEIN: BewuA.18928.a.MX152.PW39137

Molecular models

METHOD RESULTS
comparative modelling Robetta_15668
comparative modelling Robetta_15669
comparative modelling Robetta_15670
comparative modelling Robetta_15671
comparative modelling Robetta_15672
comparative modelling Robetta_15673
comparative modelling Robetta_15661
comparative modelling Robetta_15732
comparative modelling Robetta_15733
comparative modelling Robetta_15666
comparative modelling Robetta_15667

External Resources

RESOURCE REFERENCE ID
GenBank: QHD43415
UniProt: P0DTC1
ViPR: link

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESLVPGFNE KTHVQLSLPV LQVRDVLVRG FGDSVEEVLS EARQHLKDGT CGLVEVEKGV LPQLEQPYVF IKRSDARTAP HGHVMVELVA ELEGIQYGRS GETLGVLVPH VGEIPVAYRK VLLRKNGNKG AGGHSYGADL KSFDLGDELG TDPYEDFQEN WNTKHSSGVT RELMRELNGG AYTRYVDNNF CGPDGYPLEC IKDLLARAGK ASCTLSEQLD FIDTKRGVYC CREHEHEIAW YTERSEKSYE LQTPFEIKLA KKFDTFNGEC PNFVFPLNSI IKTIQPRVEK KKLDGFMGRI RSVYPVASPN ECNQMCLSTL MKCDHCGETS WQTGDFVKAT CEFCGTENLT KEGATTCGYL PQNAVVKIYC PACHNSEVGP EHSLAEYHNE SGLKTILRKG GRTIAFGGCV FSYVGCHNKC AYWVPRASAN IGCNHTGVVG EGSEGLNDNL LEILQKEKVN INIVGDFKLN EEIAIILASF SASTSAFVET VKGLDYKAFK QIVESCGNFK VTKGKAKKGA WNIGEQKSIL SPLYAFASEA ARVVRSIFSR TLETAQNSVR VLQKAAITIL DGISQYSLRL IDAMMFTSDL ATNNLVVMAY ITGGVVQLTS QWLTNIFGTV YEKLKPVLDW LEEKFKEGVE FLRDGWEIVK FISTCACEIV GGQIVTCAKE IKESVQTFFK LVNKFLALCA DSIIIGGAKL KALNLGETFV THSKGLYRKC VKSREETGLL MPLKAPKEII FLEGETLPTE VLTEEVVLKT GDLQPLEQPT SEAVEAPLVG TPVCINGLML LEIKDTEKYC ALAPNMMVTN NTFTLKGGAP TKVTFGDDTV IEVQGYKSVN ITFELDERID KVLNEKCSAY TVELGTEVNE FACVVADAVI KTLQPVSELL TPLGIDLDEW SMATYYLFDE SGEFKLASHM YCSFYPPDED EEEGDCEEEE FEPSTQYEYG TEDDYQGKPL EFGATSAALQ PEEEQEEDWL DDDSQQTVGQ QDGSEDNQTT TIQTIVEVQP QLEMELTPVV QTIEVNSFSG YLKLTDNVYI KNADIVEEAK KVKPTVVVNA ANVYLKHGGG VAGALNKATN NAMQVESDDY IATNGPLKVG GSCVLSGHNL AKHCLHVVGP NVNKGEDIQL LKSAYENFNQ HEVLLAPLLS AGIFGADPIH SLRVCVDTVR TNVYLAVFDK NLYDKLVSSF LEMKSEKQVE QKIAEIPKEE VKPFITESKP SVEQRKQDDK KIKACVEEVT TTLEETKFLT ENLLLYIDIN GNLHPDSATL VSDIDITFLK KDAPYIVGDV VQEGVLTAVV IPTKKAGGTT EMLAKALRKV PTDNYITTYP GQGLNGYTVE EAKTVLKKCK SAFYILPSII SNEKQEILGT VSWNLREMLA HAEETRKLMP VCVETKAIVS TIQRKYKGIK IQEGVVDYGA RFYFYTSKTT VASLINTLND LNETLVTMPL GYVTHGLNLE EAARYMRSLK VPATVSVSSP DAVTAYNGYL TSSSKTPEEH FIETISLAGS YKDWSYSGQS TQLGIEFLKR GDKSVYYTSN PTTFHLDGEV ITFDNLKTLL SLREVRTIKV FTTVDNINLH TQVVDMSMTY GQQFGPTYLD GADVTKIKPH NSHEGKTFYV LPNDDTLRVE AFEYYHTTDP SFLGRYMSAL NHTKKWKYPQ VNGLTSIKWA DNNCYLATAL LTLQQIELKF NPPALQDAYY RARAGEAANF CALILAYCNK TVGELGDVRE TMSYLFQHAN LDSCKRVLNV VCKTCGQQQT TLKGVEAVMY MGTLSYEQFK KGVQIPCTCG KQATKYLVQQ ESPFVMMSAP PAQYELKHGT FTCASEYTGN YQCGHYKHIT SKETLYCIDG ALLTKSSEYK GPITDVFYKE NSYTTTIKPV TYKLDGVVCT EIDPKLDNYY KKDNSYFTEQ PIDLVPNQPY PNASFDNFKF VCDNIKFADD LNQLTGYKKP ASRELKVTFF PDLNGDVVAI DYKHYTPSFK KGAKLLHKPI VWHVNNATNK ATYKPNTWCI RCLWSTKPVE TSNSFDVLKS EDAQGMDNLA CEDLKPVSEE VVENPTIQKD VLECNVKTTE VVGDIILKPA NNSLKITEEV GHTDLMAAYV DNSSLTIKKP NELSRVLGLK TLATHGLAAV NSVPWDTIAN YAKPFLNKVV STTTNIVTRC LNRVCTNYMP YFFTLLLQLC TFTRSTNSRI KASMPTTIAK NTVKSVGKFC LEASFNYLKS PNFSKLINII IWFLLLSVCL GSLIYSTAAL GVLMSNLGMP SYCTGYREGY LNSTNVTIAT YCTGSIPCSV CLSGLDSLDT YPSLETIQIT ISSFKWDLTA FGLVAEWFLA YILFTRFFYV LGLAAIMQLF FSYFAVHFIS NSWLMWLIIN LVQMAPISAM VRMYIFFASF YYVWKSYVHV VDGCNSSTCM MCYKRNRATR VECTTIVNGV RRSFYVYANG GKGFCKLHNW NCVNCDTFCA GSTFISDEVA RDLSLQFKRP INPTDQSSYI VDSVTVKNGS IHLYFDKAGQ KTYERHSLSH FVNLDNLRAN NTKGSLPINV IVFDGKSKCE ESSAKSASVY YSQLMCQPIL LLDQALVSDV GDSAEVAVKM FDAYVNTFSS TFNVPMEKLK TLVATAEAEL AKNVSLDNVL STFISAARQG FVDSDVETKD VVECLKLSHQ SDIEVTGDSC NNYMLTYNKV ENMTPRDLGA CIDCSARHIN AQVAKSHNIA LIWNVKDFMS LSEQLRKQIR SAAKKNNLPF KLTCATTRQV VNVVTTKIAL KGGKIVNNWL KQLIKVTLVF LFVAAIFYLI TPVHVMSKHT DFSSEIIGYK AIDGGVTRDI ASTDTCFANK HADFDTWFSQ RGGSYTNDKA CPLIAAVITR EVGFVVPGLP GTILRTTNGD FLHFLPRVFS AVGNICYTPS KLIEYTDFAT SACVLAAECT IFKDASGKPV PYCYDTNVLE GSVAYESLRP DTRYVLMDGS IIQFPNTYLE GSVRVVTTFD SEYCRHGTCE RSEAGVCVST SGRWVLNNDY YRSLPGVFCG VDAVNLLTNM FTPLIQPIGA LDISASIVAG GIVAIVVTCL AYYFMRFRRA FGEYSHVVAF NTLLFLMSFT VLCLTPVYSF LPGVYSVIYL YLTFYLTNDV SFLAHIQWMV MFTPLVPFWI TIAYIICIST KHFYWFFSNY LKRRVVFNGV SFSTFEEAAL CTFLLNKEMY LKLRSDVLLP LTQYNRYLAL YNKYKYFSGA MDTTSYREAA CCHLAKALND FSNSGSDVLY QPPQTSITSA VLQSGFRKMA FPSGKVEGCM VQVTCGTTTL NGLWLDDVVY CPRHVICTSE DMLNPNYEDL LIRKSNHNFL VQAGNVQLRV IGHSMQNCVL KLKVDTANPK TPKYKFVRIQ PGQTFSVLAC YNGSPSGVYQ CAMRPNFTIK GSFLNGSCGS VGFNIDYDCV SFCYMHHMEL PTGVHAGTDL EGNFYGPFVD RQTAQAAGTD TTITVNVLAW LYAAVINGDR WFLNRFTTTL NDFNLVAMKY NYEPLTQDHV DILGPLSAQT GIAVLDMCAS LKELLQNGMN GRTILGSALL EDEFTPFDVV RQCSGVTFQS AVKRTIKGTH HWLLLTILTS LLVLVQSTQW SLFFFLYENA FLPFAMGIIA MSAFAMMFVK HKHAFLCLFL LPSLATVAYF NMVYMPASWV MRIMTWLDMV DTSLSGFKLK DCVMYASAVV LLILMTARTV YDDGARRVWT LMNVLTLVYK VYYGNALDQA ISMWALIISV TSNYSGVVTT VMFLARGIVF MCVEYCPIFF ITGNTLQCIM LVYCFLGYFC TCYFGLFCLL NRYFRLTLGV YDYLVSTQEF RYMNSQGLLP PKNSIDAFKL NIKLLGVGGK PCIKVATVQS KMSDVKCTSV VLLSVLQQLR VESSSKLWAQ CVQLHNDILL AKDTTEAFEK MVSLLSVLLS MQGAVDINKL CEEMLDNRAT LQAIASEFSS LPSYAAFATA QEAYEQAVAN GDSEVVLKKL KKSLNVAKSE FDRDAAMQRK LEKMADQAMT QMYKQARSED KRAKVTSAMQ TMLFTMLRKL DNDALNNIIN NARDGCVPLN IIPLTTAAKL MVVIPDYNTY KNTCDGTTFT YASALWEIQQ VVDADSKIVQ LSEISMDNSP NLAWPLIVTA LRANSAVKLQ NNELSPVALR QMSCAAGTTQ TACTDDNALA YYNTTKGGRF VLALLSDLQD LKWARFPKSD GTGTIYTELE PPCRFVTDTP KGPKVKYLYF IKGLNNLNRG MVLGSLAATV RLQAGNATEV PANSTVLSFC AFAVDAAKAY KDYLASGGQP ITNCVKMLCT HTGTGQAITV TPEANMDQES FGGASCCLYC RCHIDHPNPK GFCDLKGKYV QIPTTCANDP VGFTLKNTVC TVCGMWKGYG CSCDQLREPM LQSADAQSFL NRVCGVSAAR LTPCGTGTST DVVYRAFDIY NDKVAGFAKF LKTNCCRFQE KDEDDNLIDS YFVVKRHTFS NYQHEETIYN LLKDCPAVAK HDFFKFRIDG DMVPHISRQR LTKYTMADLV YALRHFDEGN CDTLKEILVT YNCCDDDYFN KKDWYDFVEN PDILRVYANL GERVRQALLK TVQFCDAMRN AGIVGVLTLD NQDLNGNWYD FGDFIQTTPG SGVPVVDSYY SLLMPILTLT RALTAESHVD TDLTKPYIKW DLLKYDFTEE RLKLFDRYFK YWDQTYHPNC VNCLDDRCIL HCANFNVLFS TVFPPTSFGP LVRKIFVDGV PFVVSTGYHF RELGVVHNQD VNLHSSRLSF KELLVYAADP AMHAASGNLL LDKRTTCFSV AALTNNVAFQ TVKPGNFNKD FYDFAVSKGF FKEGSSVELK HFFFAQDGNA AISDYDYYRY NLPTMCDIRQ LLFVVEVVDK YFDCYDGGCI NANQVIVNNL DKSAGFPFNK WGKARLYYDS MSYEDQDALF AYTKRNVIPT ITQMNLKYAI SAKNRARTVA GVSICSTMTN RQFHQKLLKS IAATRGATVV IGTSKFYGGW HNMLKTVYSD VENPHLMGWD YPKCDRAMPN MLRIMASLVL ARKHTTCCSL SHRFYRLANE CAQVLSEMVM CGGSLYVKPG GTSSGDATTA YANSVFNICQ AVTANVNALL STDGNKIADK YVRNLQHRLY ECLYRNRDVD TDFVNEFYAY LRKHFSMMIL SDDAVVCFNS TYASQGLVAS IKNFKSVLYY QNNVFMSEAK CWTETDLTKG PHEFCSQHTM LVKQGDDYVY LPYPDPSRIL GAGCFVDDIV KTDGTLMIER FVSLAIDAYP LTKHPNQEYA DVFHLYLQYI RKLHDELTGH MLDMYSVMLT NDNTSRYWEP EFYEAMYTPH TVLQAVGACV LCNSQTSLRC GACIRRPFLC CKCCYDHVIS TSHKLVLSVN PYVCNAPGCD VTDVTQLYLG GMSYYCKSHK PPISFPLCAN GQVFGLYKNT CVGSDNVTDF NAIATCDWTN AGDYILANTC TERLKLFAAE TLKATEETFK LSYGIATVRE VLSDRELHLS WEVGKPRPPL NRNYVFTGYR VTKNSKVQIG EYTFEKGDYG DAVVYRGTTT YKLNVGDYFV LTSHTVMPLS APTLVPQEHY VRITGLYPTL NISDEFSSNV ANYQKVGMQK YSTLQGPPGT GKSHFAIGLA LYYPSARIVY TACSHAAVDA LCEKALKYLP IDKCSRIIPA RARVECFDKF KVNSTLEQYV FCTVNALPET TADIVVFDEI SMATNYDLSV VNARLRAKHY VYIGDPAQLP APRTLLTKGT LEPEYFNSVC RLMKTIGPDM FLGTCRRCPA EIVDTVSALV YDNKLKAHKD KSAQCFKMFY KGVITHDVSS AINRPQIGVV REFLTRNPAW RKAVFISPYN SQNAVASKIL GLPTQTVDSS QGSEYDYVIF TQTTETAHSC NVNRFNVAIT RAKVGILCIM SDRDLYDKLQ FTSLEIPRRN VATLQAENVT GLFKDCSKVI TGLHPTQAPT HLSVDTKFKT EGLCVDIPGI PKDMTYRRLI SMMGFKMNYQ VNGYPNMFIT REEAIRHVRA WIGFDVEGCH ATREAVGTNL PLQLGFSTGV NLVAVPTGYV DTPNNTDFSR VSAKPPPGDQ FKHLIPLMYK GLPWNVVRIK IVQMLSDTLK NLSDRVVFVL WAHGFELTSM KYFVKIGPER TCCLCDRRAT CFSTASDTYA CWHHSIGFDY VYNPFMIDVQ QWGFTGNLQS NHDLYCQVHG NAHVASCDAI MTRCLAVHEC FVKRVDWTIE YPIIGDELKI NAACRKVQHM VVKAALLADK FPVLHDIGNP KAIKCVPQAD VEWKFYDAQP CSDKAYKIEE LFYSYATHSD KFTDGVCLFW NCNVDRYPAN SIVCRFDTRV LSNLNLPGCD GGSLYVNKHA FHTPAFDKSA FVNLKQLPFF YYSDSPCESH GKQVVSDIDY VPLKSATCIT RCNLGGAVCR HHANEYRLYL DAYNMMISAG FSLWVYKQFD TYNLWNTFTR LQSLENVAFN VVNKGHFDGQ QGEVPVSIIN NTVYTKVDGV DVELFENKTT LPVNVAFELW AKRNIKPVPE VKILNNLGVD IAANTVIWDY KRDAPAHIST IGVCSMTDIA KKPTETICAP LTVFFDGRVD GQVDLFRNAR NGVLITEGSV KGLQPSVGPK QASLNGVTLI GEAVKTQFNY YKKVDGVVQQ LPETYFTQSR NLQEFKPRSQ MEIDFLELAM DEFIERYKLE GYAFEHIVYG DFSHSQLGGL HLLIGLAKRF KESPFELEDF IPMDSTVKNY FITDAQTGSS KCVCSVIDLL LDDFVEIIKS QDLSVVSKVV KVTIDYTEIS FMLWCKDGHV ETFYPKLQSS QAWQPGVAMP NLYKMQRMLL EKCDLQNYGD SATLPKGIMM NVAKYTQLCQ YLNTLTLAVP YNMRVIHFGA GSDKGVAPGT AVLRQWLPTG TLLVDSDLND FVSDADSTLI GDCATVHTAN KWDLIISDMY DPKTKNVTKE NDSKEGFFTY ICGFIQQKLA LGGSVAIKIT EHSWNADLYK LMGHFAWWTA FVTNVNASSS EAFLIGCNYL GKPREQIDGY VMHANYIFWR NTNPIQLSSY SLFDMSKFPL KLRGTAVMSL KEGQINDMIL SLLSKGRLII RENNRVVISS DVLVNN
NT Sequence
ATGGAGAGCC TTGTCCCTGG TTTCAACGAG AAAACACACG TCCAACTCAG TTTGCCTGTT TTACAGGTTC GCGACGTGCT CGTACGTGGC TTTGGAGACT CCGTGGAGGA GGTCTTATCA GAGGCACGTC AACATCTTAA AGATGGCACT TGTGGCTTAG TAGAAGTTGA AAAAGGCGTT TTGCCTCAAC TTGAACAGCC CTATGTGTTC ATCAAACGTT CGGATGCTCG AACTGCACCT CATGGTCATG TTATGGTTGA GCTGGTAGCA GAACTCGAAG GCATTCAGTA CGGTCGTAGT GGTGAGACAC TTGGTGTCCT TGTCCCTCAT GTGGGCGAAA TACCAGTGGC TTACCGCAAG GTTCTTCTTC GTAAGAACGG TAATAAAGGA GCTGGTGGCC ATAGTTACGG CGCCGATCTA AAGTCATTTG ACTTAGGCGA CGAGCTTGGC ACTGATCCTT ATGAAGATTT TCAAGAAAAC TGGAACACTA AACATAGCAG TGGTGTTACC CGTGAACTCA TGCGTGAGCT TAACGGAGGG GCATACACTC GCTATGTCGA TAACAACTTC TGTGGCCCTG ATGGCTACCC TCTTGAGTGC ATTAAAGACC TTCTAGCACG TGCTGGTAAA GCTTCATGCA CTTTGTCCGA ACAACTGGAC TTTATTGACA CTAAGAGGGG TGTATACTGC TGCCGTGAAC ATGAGCATGA AATTGCTTGG TACACGGAAC GTTCTGAAAA GAGCTATGAA TTGCAGACAC CTTTTGAAAT TAAATTGGCA AAGAAATTTG ACACCTTCAA TGGGGAATGT CCAAATTTTG TATTTCCCTT AAATTCCATA ATCAAGACTA TTCAACCAAG GGTTGAAAAG AAAAAGCTTG ATGGCTTTAT GGGTAGAATT CGATCTGTCT ATCCAGTTGC GTCACCAAAT GAATGCAACC AAATGTGCCT TTCAACTCTC ATGAAGTGTG ATCATTGTGG TGAAACTTCA TGGCAGACGG GCGATTTTGT TAAAGCCACT TGCGAATTTT GTGGCACTGA GAATTTGACT AAAGAAGGTG CCACTACTTG TGGTTACTTA CCCCAAAATG CTGTTGTTAA AATTTATTGT CCAGCATGTC ACAATTCAGA AGTAGGACCT GAGCATAGTC TTGCCGAATA CCATAATGAA TCTGGCTTGA AAACCATTCT TCGTAAGGGT GGTCGCACTA TTGCCTTTGG AGGCTGTGTG TTCTCTTATG TTGGTTGCCA TAACAAGTGT GCCTATTGGG TTCCACGTGC TAGCGCTAAC ATAGGTTGTA ACCATACAGG TGTTGTTGGA GAAGGTTCCG AAGGTCTTAA TGACAACCTT CTTGAAATAC TCCAAAAAGA GAAAGTCAAC ATCAATATTG TTGGTGACTT TAAACTTAAT GAAGAGATCG CCATTATTTT GGCATCTTTT TCTGCTTCCA CAAGTGCTTT TGTGGAAACT GTGAAAGGTT TGGATTATAA AGCATTCAAA CAAATTGTTG AATCCTGTGG TAATTTTAAA GTTACAAAAG GAAAAGCTAA AAAAGGTGCC TGGAATATTG GTGAACAGAA ATCAATACTG AGTCCTCTTT ATGCATTTGC ATCAGAGGCT GCTCGTGTTG TACGATCAAT TTTCTCCCGC ACTCTTGAAA CTGCTCAAAA TTCTGTGCGT GTTTTACAGA AGGCCGCTAT AACAATACTA GATGGAATTT CACAGTATTC ACTGAGACTC ATTGATGCTA TGATGTTCAC ATCTGATTTG GCTACTAACA ATCTAGTTGT AATGGCCTAC ATTACAGGTG GTGTTGTTCA GTTGACTTCG CAGTGGCTAA CTAACATCTT TGGCACTGTT TATGAAAAAC TCAAACCCGT CCTTGATTGG CTTGAAGAGA AGTTTAAGGA AGGTGTAGAG TTTCTTAGAG ACGGTTGGGA AATTGTTAAA TTTATCTCAA CCTGTGCTTG TGAAATTGTC GGTGGACAAA TTGTCACCTG TGCAAAGGAA ATTAAGGAGA GTGTTCAGAC ATTCTTTAAG CTTGTAAATA AATTTTTGGC TTTGTGTGCT GACTCTATCA TTATTGGTGG AGCTAAACTT AAAGCCTTGA ATTTAGGTGA AACATTTGTC ACGCACTCAA AGGGATTGTA CAGAAAGTGT GTTAAATCCA GAGAAGAAAC TGGCCTACTC ATGCCTCTAA AAGCCCCAAA AGAAATTATC TTCTTAGAGG GAGAAACACT TCCCACAGAA GTGTTAACAG AGGAAGTTGT CTTGAAAACT GGTGATTTAC AACCATTAGA ACAACCTACT AGTGAAGCTG TTGAAGCTCC ATTGGTTGGT ACACCAGTTT GTATTAACGG GCTTATGTTG CTCGAAATCA AAGACACAGA AAAGTACTGT GCCCTTGCAC CTAATATGAT GGTAACAAAC AATACCTTCA CACTCAAAGG CGGTGCACCA ACAAAGGTTA CTTTTGGTGA TGACACTGTG ATAGAAGTGC AAGGTTACAA GAGTGTGAAT ATCACTTTTG AACTTGATGA AAGGATTGAT AAAGTACTTA ATGAGAAGTG CTCTGCCTAT ACAGTTGAAC TCGGTACAGA AGTAAATGAG TTCGCCTGTG TTGTGGCAGA TGCTGTCATA AAAACTTTGC AACCAGTATC TGAATTACTT ACACCACTGG GCATTGATTT AGATGAGTGG AGTATGGCTA CATACTACTT ATTTGATGAG TCTGGTGAGT TTAAATTGGC TTCACATATG TATTGTTCTT TCTACCCTCC AGATGAGGAT GAAGAAGAAG GTGATTGTGA AGAAGAAGAG TTTGAGCCAT CAACTCAATA TGAGTATGGT ACTGAAGATG ATTACCAAGG TAAACCTTTG GAATTTGGTG CCACTTCTGC TGCTCTTCAA CCTGAAGAAG AGCAAGAAGA AGATTGGTTA GATGATGATA GTCAACAAAC TGTTGGTCAA CAAGACGGCA GTGAGGACAA TCAGACAACT ACTATTCAAA CAATTGTTGA GGTTCAACCT CAATTAGAGA TGGAACTTAC ACCAGTTGTT CAGACTATTG AAGTGAATAG TTTTAGTGGT TATTTAAAAC TTACTGACAA TGTATACATT AAAAATGCAG ACATTGTGGA AGAAGCTAAA AAGGTAAAAC CAACAGTGGT TGTTAATGCA GCCAATGTTT ACCTTAAACA TGGAGGAGGT GTTGCAGGAG CCTTAAATAA GGCTACTAAC AATGCCATGC AAGTTGAATC TGATGATTAC ATAGCTACTA ATGGACCACT TAAAGTGGGT GGTAGTTGTG TTTTAAGCGG ACACAATCTT GCTAAACACT GTCTTCATGT TGTCGGCCCA AATGTTAACA AAGGTGAAGA CATTCAACTT CTTAAGAGTG CTTATGAAAA TTTTAATCAG CACGAAGTTC TACTTGCACC ATTATTATCA GCTGGTATTT TTGGTGCTGA CCCTATACAT TCTTTAAGAG TTTGTGTAGA TACTGTTCGC ACAAATGTCT ACTTAGCTGT CTTTGATAAA AATCTCTATG ACAAACTTGT TTCAAGCTTT TTGGAAATGA AGAGTGAAAA GCAAGTTGAA CAAAAGATCG CTGAGATTCC TAAAGAGGAA GTTAAGCCAT TTATAACTGA AAGTAAACCT TCAGTTGAAC AGAGAAAACA AGATGATAAG AAAATCAAAG CTTGTGTTGA AGAAGTTACA ACAACTCTGG AAGAAACTAA GTTCCTCACA GAAAACTTGT TACTTTATAT TGACATTAAT GGCAATCTTC ATCCAGATTC TGCCACTCTT GTTAGTGACA TTGACATCAC TTTCTTAAAG AAAGATGCTC CATATATAGT GGGTGATGTT GTTCAAGAGG GTGTTTTAAC TGCTGTGGTT ATACCTACTA AAAAGGCTGG TGGCACTACT GAAATGCTAG CGAAAGCTTT GAGAAAAGTG CCAACAGACA ATTATATAAC CACTTACCCG GGTCAGGGTT TAAATGGTTA CACTGTAGAG GAGGCAAAGA CAGTGCTTAA AAAGTGTAAA AGTGCCTTTT ACATTCTACC ATCTATTATC TCTAATGAGA AGCAAGAAAT TCTTGGAACT GTTTCTTGGA ATTTGCGAGA AATGCTTGCA CATGCAGAAG AAACACGCAA ATTAATGCCT GTCTGTGTGG AAACTAAAGC CATAGTTTCA ACTATACAGC GTAAATATAA GGGTATTAAA ATACAAGAGG GTGTGGTTGA TTATGGTGCT AGATTTTACT TTTACACCAG TAAAACAACT GTAGCGTCAC TTATCAACAC ACTTAACGAT CTAAATGAAA CTCTTGTTAC AATGCCACTT GGCTATGTAA CACATGGCTT AAATTTGGAA GAAGCTGCTC GGTATATGAG ATCTCTCAAA GTGCCAGCTA CAGTTTCTGT TTCTTCACCT GATGCTGTTA CAGCGTATAA TGGTTATCTT ACTTCTTCTT CTAAAACACC TGAAGAACAT TTTATTGAAA CCATCTCACT TGCTGGTTCC TATAAAGATT GGTCCTATTC TGGACAATCT ACACAACTAG GTATAGAATT TCTTAAGAGA GGTGATAAAA GTGTATATTA CACTAGTAAT CCTACCACAT TCCACCTAGA TGGTGAAGTT ATCACCTTTG ACAATCTTAA GACACTTCTT TCTTTGAGAG AAGTGAGGAC TATTAAGGTG TTTACAACAG TAGACAACAT TAACCTCCAC ACGCAAGTTG TGGACATGTC AATGACATAT GGACAACAGT TTGGTCCAAC TTATTTGGAT GGAGCTGATG TTACTAAAAT AAAACCTCAT AATTCACATG AAGGTAAAAC ATTTTATGTT TTACCTAATG ATGACACTCT ACGTGTTGAG GCTTTTGAGT ACTACCACAC AACTGATCCT AGTTTTCTGG GTAGGTACAT GTCAGCATTA AATCACACTA AAAAGTGGAA ATACCCACAA GTTAATGGTT TAACTTCTAT TAAATGGGCA GATAACAACT GTTATCTTGC CACTGCATTG TTAACACTCC AACAAATAGA GTTGAAGTTT AATCCACCTG CTCTACAAGA TGCTTATTAC AGAGCAAGGG CTGGTGAAGC TGCTAACTTT TGTGCACTTA TCTTAGCCTA CTGTAATAAG ACAGTAGGTG AGTTAGGTGA TGTTAGAGAA ACAATGAGTT ACTTGTTTCA ACATGCCAAT TTAGATTCTT GCAAAAGAGT CTTGAACGTG GTGTGTAAAA CTTGTGGACA ACAGCAGACA ACCCTTAAGG GTGTAGAAGC TGTTATGTAC ATGGGCACAC TTTCTTATGA ACAATTTAAG AAAGGTGTTC AGATACCTTG TACGTGTGGT AAACAAGCTA CAAAATATCT AGTACAACAG GAGTCACCTT TTGTTATGAT GTCAGCACCA CCTGCTCAGT ATGAACTTAA GCATGGTACA TTTACTTGTG CTAGTGAGTA CACTGGTAAT TACCAGTGTG GTCACTATAA ACATATAACT TCTAAAGAAA CTTTGTATTG CATAGACGGT GCTTTACTTA CAAAGTCCTC AGAATACAAA GGTCCTATTA CGGATGTTTT CTACAAAGAA AACAGTTACA CAACAACCAT AAAACCAGTT ACTTATAAAT TGGATGGTGT TGTTTGTACA GAAATTGACC CTAAGTTGGA CAATTATTAT AAGAAAGACA ATTCTTATTT CACAGAGCAA CCAATTGATC TTGTACCAAA CCAACCATAT CCAAACGCAA GCTTCGATAA TTTTAAGTTT GTATGTGATA ATATCAAATT TGCTGATGAT TTAAACCAGT TAACTGGTTA TAAGAAACCT GCTTCAAGAG AGCTTAAAGT TACATTTTTC CCTGACTTAA ATGGTGATGT GGTGGCTATT GATTATAAAC ACTACACACC CTCTTTTAAG AAAGGAGCTA AATTGTTACA TAAACCTATT GTTTGGCATG TTAACAATGC AACTAATAAA GCCACGTATA AACCAAATAC CTGGTGTATA CGTTGTCTTT GGAGCACAAA ACCAGTTGAA ACATCAAATT CGTTTGATGT ACTGAAGTCA GAGGACGCGC AGGGAATGGA TAATCTTGCC TGCGAAGATC TAAAACCAGT CTCTGAAGAA GTAGTGGAAA ATCCTACCAT ACAGAAAGAC GTTCTTGAGT GTAATGTGAA AACTACCGAA GTTGTAGGAG ACATTATACT TAAACCAGCA AATAATAGTT TAAAAATTAC AGAAGAGGTT GGCCACACAG ATCTAATGGC TGCTTATGTA GACAATTCTA GTCTTACTAT TAAGAAACCT AATGAATTAT CTAGAGTATT AGGTTTGAAA ACCCTTGCTA CTCATGGTTT AGCTGCTGTT AATAGTGTCC CTTGGGATAC TATAGCTAAT TATGCTAAGC CTTTTCTTAA CAAAGTTGTT AGTACAACTA CTAACATAGT TACACGGTGT TTAAACCGTG TTTGTACTAA TTATATGCCT TATTTCTTTA CTTTATTGCT ACAATTGTGT ACTTTTACTA GAAGTACAAA TTCTAGAATT AAAGCATCTA TGCCGACTAC TATAGCAAAG AATACTGTTA AGAGTGTCGG TAAATTTTGT CTAGAGGCTT CATTTAATTA TTTGAAGTCA CCTAATTTTT CTAAACTGAT AAATATTATA ATTTGGTTTT TACTATTAAG TGTTTGCCTA GGTTCTTTAA TCTACTCAAC CGCTGCTTTA GGTGTTTTAA TGTCTAATTT AGGCATGCCT TCTTACTGTA CTGGTTACAG AGAAGGCTAT TTGAACTCTA CTAATGTCAC TATTGCAACC TACTGTACTG GTTCTATACC TTGTAGTGTT TGTCTTAGTG GTTTAGATTC TTTAGACACC TATCCTTCTT TAGAAACTAT ACAAATTACC ATTTCATCTT TTAAATGGGA TTTAACTGCT TTTGGCTTAG TTGCAGAGTG GTTTTTGGCA TATATTCTTT TCACTAGGTT TTTCTATGTA CTTGGATTGG CTGCAATCAT GCAATTGTTT TTCAGCTATT TTGCAGTACA TTTTATTAGT AATTCTTGGC TTATGTGGTT AATAATTAAT CTTGTACAAA TGGCCCCGAT TTCAGCTATG GTTAGAATGT ACATCTTCTT TGCATCATTT TATTATGTAT GGAAAAGTTA TGTGCATGTT GTAGACGGTT GTAATTCATC AACTTGTATG ATGTGTTACA AACGTAATAG AGCAACAAGA GTCGAATGTA CAACTATTGT TAATGGTGTT AGAAGGTCCT TTTATGTCTA TGCTAATGGA GGTAAAGGCT TTTGCAAACT ACACAATTGG AATTGTGTTA ATTGTGATAC ATTCTGTGCT GGTAGTACAT TTATTAGTGA TGAAGTTGCG AGAGACTTGT CACTACAGTT TAAAAGACCA ATAAATCCTA CTGACCAGTC TTCTTACATC GTTGATAGTG TTACAGTGAA GAATGGTTCC ATCCATCTTT ACTTTGATAA AGCTGGTCAA AAGACTTATG AAAGACATTC TCTCTCTCAT TTTGTTAACT TAGACAACCT GAGAGCTAAT AACACTAAAG GTTCATTGCC TATTAATGTT ATAGTTTTTG ATGGTAAATC AAAATGTGAA GAATCATCTG CAAAATCAGC GTCTGTTTAC TACAGTCAGC TTATGTGTCA ACCTATACTG TTACTAGATC AGGCATTAGT GTCTGATGTT GGTGATAGTG CGGAAGTTGC AGTTAAAATG TTTGATGCTT ACGTTAATAC GTTTTCATCA ACTTTTAACG TACCAATGGA AAAACTCAAA ACACTAGTTG CAACTGCAGA AGCTGAACTT GCAAAGAATG TGTCCTTAGA CAATGTCTTA TCTACTTTTA TTTCAGCAGC TCGGCAAGGG TTTGTTGATT CAGATGTAGA AACTAAAGAT GTTGTTGAAT GTCTTAAATT GTCACATCAA TCTGACATAG AAGTTACTGG CGATAGTTGT AATAACTATA TGCTCACCTA TAACAAAGTT GAAAACATGA CACCCCGTGA CCTTGGTGCT TGTATTGACT GTAGTGCGCG TCATATTAAT GCGCAGGTAG CAAAAAGTCA CAACATTGCT TTGATATGGA ACGTTAAAGA TTTCATGTCA TTGTCTGAAC AACTACGAAA ACAAATACGT AGTGCTGCTA AAAAGAATAA CTTACCTTTT AAGTTGACAT GTGCAACTAC TAGACAAGTT GTTAATGTTG TAACAACAAA GATAGCACTT AAGGGTGGTA AAATTGTTAA TAATTGGTTG AAGCAGTTAA TTAAAGTTAC ACTTGTGTTC CTTTTTGTTG CTGCTATTTT CTATTTAATA ACACCTGTTC ATGTCATGTC TAAACATACT GACTTTTCAA GTGAAATCAT AGGATACAAG GCTATTGATG GTGGTGTCAC TCGTGACATA GCATCTACAG ATACTTGTTT TGCTAACAAA CATGCTGATT TTGACACATG GTTTAGCCAG CGTGGTGGTA GTTATACTAA TGACAAAGCT TGCCCATTGA TTGCTGCAGT CATAACAAGA GAAGTGGGTT TTGTCGTGCC TGGTTTGCCT GGCACGATAT TACGCACAAC TAATGGTGAC TTTTTGCATT TCTTACCTAG AGTTTTTAGT GCAGTTGGTA ACATCTGTTA CACACCATCA AAACTTATAG AGTACACTGA CTTTGCAACA TCAGCTTGTG TTTTGGCTGC TGAATGTACA ATTTTTAAAG ATGCTTCTGG TAAGCCAGTA CCATATTGTT ATGATACCAA TGTACTAGAA GGTTCTGTTG CTTATGAAAG TTTACGCCCT GACACACGTT ATGTGCTCAT GGATGGCTCT ATTATTCAAT TTCCTAACAC CTACCTTGAA GGTTCTGTTA GAGTGGTAAC AACTTTTGAT TCTGAGTACT GTAGGCACGG CACTTGTGAA AGATCAGAAG CTGGTGTTTG TGTATCTACT AGTGGTAGAT GGGTACTTAA CAATGATTAT TACAGATCTT TACCAGGAGT TTTCTGTGGT GTAGATGCTG TAAATTTACT TACTAATATG TTTACACCAC TAATTCAACC TATTGGTGCT TTGGACATAT CAGCATCTAT AGTAGCTGGT GGTATTGTAG CTATCGTAGT AACATGCCTT GCCTACTATT TTATGAGGTT TAGAAGAGCT TTTGGTGAAT ACAGTCATGT AGTTGCCTTT AATACTTTAC TATTCCTTAT GTCATTCACT GTACTCTGTT TAACACCAGT TTACTCATTC TTACCTGGTG TTTATTCTGT TATTTACTTG TACTTGACAT TTTATCTTAC TAATGATGTT TCTTTTTTAG CACATATTCA GTGGATGGTT ATGTTCACAC CTTTAGTACC TTTCTGGATA ACAATTGCTT ATATCATTTG TATTTCCACA AAGCATTTCT ATTGGTTCTT TAGTAATTAC CTAAAGAGAC GTGTAGTCTT TAATGGTGTT TCCTTTAGTA CTTTTGAAGA AGCTGCGCTG TGCACCTTTT TGTTAAATAA AGAAATGTAT CTAAAGTTGC GTAGTGATGT GCTATTACCT CTTACGCAAT ATAATAGATA CTTAGCTCTT TATAATAAGT ACAAGTATTT TAGTGGAGCA ATGGATACAA CTAGCTACAG AGAAGCTGCT TGTTGTCATC TCGCAAAGGC TCTCAATGAC TTCAGTAACT CAGGTTCTGA TGTTCTTTAC CAACCACCAC AAACCTCTAT CACCTCAGCT GTTTTGCAGA GTGGTTTTAG AAAAATGGCA TTCCCATCTG GTAAAGTTGA GGGTTGTATG GTACAAGTAA CTTGTGGTAC AACTACACTT AACGGTCTTT GGCTTGATGA CGTAGTTTAC TGTCCAAGAC ATGTGATCTG CACCTCTGAA GACATGCTTA ACCCTAATTA TGAAGATTTA CTCATTCGTA AGTCTAATCA TAATTTCTTG GTACAGGCTG GTAATGTTCA ACTCAGGGTT ATTGGACATT CTATGCAAAA TTGTGTACTT AAGCTTAAGG TTGATACAGC CAATCCTAAG ACACCTAAGT ATAAGTTTGT TCGCATTCAA CCAGGACAGA CTTTTTCAGT GTTAGCTTGT TACAATGGTT CACCATCTGG TGTTTACCAA TGTGCTATGA GGCCCAATTT CACTATTAAG GGTTCATTCC TTAATGGTTC ATGTGGTAGT GTTGGTTTTA ACATAGATTA TGACTGTGTC TCTTTTTGTT ACATGCACCA TATGGAATTA CCAACTGGAG TTCATGCTGG CACAGACTTA GAAGGTAACT TTTATGGACC TTTTGTTGAC AGGCAAACAG CACAAGCAGC TGGTACGGAC ACAACTATTA CAGTTAATGT TTTAGCTTGG TTGTACGCTG CTGTTATAAA TGGAGACAGG TGGTTTCTCA ATCGATTTAC CACAACTCTT AATGACTTTA ACCTTGTGGC TATGAAGTAC AATTATGAAC CTCTAACACA AGACCATGTT GACATACTAG GACCTCTTTC TGCTCAAACT GGAATTGCCG TTTTAGATAT GTGTGCTTCA TTAAAAGAAT TACTGCAAAA TGGTATGAAT GGACGTACCA TATTGGGTAG TGCTTTATTA GAAGATGAAT TTACACCTTT TGATGTTGTT AGACAATGCT CAGGTGTTAC TTTCCAAAGT GCAGTGAAAA GAACAATCAA GGGTACACAC CACTGGTTGT TACTCACAAT TTTGACTTCA CTTTTAGTTT TAGTCCAGAG TACTCAATGG TCTTTGTTCT TTTTTTTGTA TGAAAATGCC TTTTTACCTT TTGCTATGGG TATTATTGCT ATGTCTGCTT TTGCAATGAT GTTTGTCAAA CATAAGCATG CATTTCTCTG TTTGTTTTTG TTACCTTCTC TTGCCACTGT AGCTTATTTT AATATGGTCT ATATGCCTGC TAGTTGGGTG ATGCGTATTA TGACATGGTT GGATATGGTT GATACTAGTT TGTCTGGTTT TAAGCTAAAA GACTGTGTTA TGTATGCATC AGCTGTAGTG TTACTAATCC TTATGACAGC AAGAACTGTG TATGATGATG GTGCTAGGAG AGTGTGGACA CTTATGAATG TCTTGACACT CGTTTATAAA GTTTATTATG GTAATGCTTT AGATCAAGCC ATTTCCATGT GGGCTCTTAT AATCTCTGTT ACTTCTAACT ACTCAGGTGT AGTTACAACT GTCATGTTTT TGGCCAGAGG TATTGTTTTT ATGTGTGTTG AGTATTGCCC TATTTTCTTC ATAACTGGTA ATACACTTCA GTGTATAATG CTAGTTTATT GTTTCTTAGG CTATTTTTGT ACTTGTTACT TTGGCCTCTT TTGTTTACTC AACCGCTACT TTAGACTGAC TCTTGGTGTT TATGATTACT TAGTTTCTAC ACAGGAGTTT AGATATATGA ATTCACAGGG ACTACTCCCA CCCAAGAATA GCATAGATGC CTTCAAACTC AACATTAAAT TGTTGGGTGT TGGTGGCAAA CCTTGTATCA AAGTAGCCAC TGTACAGTCT AAAATGTCAG ATGTAAAGTG CACATCAGTA GTCTTACTCT CAGTTTTGCA ACAACTCAGA GTAGAATCAT CATCTAAATT GTGGGCTCAA TGTGTCCAGT TACACAATGA CATTCTCTTA GCTAAAGATA CTACTGAAGC CTTTGAAAAA ATGGTTTCAC TACTTTCTGT TTTGCTTTCC ATGCAGGGTG CTGTAGACAT AAACAAGCTT TGTGAAGAAA TGCTGGACAA CAGGGCAACC TTACAAGCTA TAGCCTCAGA GTTTAGTTCC CTTCCATCAT ATGCAGCTTT TGCTACTGCT CAAGAAGCTT ATGAGCAGGC TGTTGCTAAT GGTGATTCTG AAGTTGTTCT TAAAAAGTTG AAGAAGTCTT TGAATGTGGC TAAATCTGAA TTTGACCGTG ATGCAGCCAT GCAACGTAAG TTGGAAAAGA TGGCTGATCA AGCTATGACC CAAATGTATA AACAGGCTAG ATCTGAGGAC AAGAGGGCAA AAGTTACTAG TGCTATGCAG ACAATGCTTT TCACTATGCT TAGAAAGTTG GATAATGATG CACTCAACAA CATTATCAAC AATGCAAGAG ATGGTTGTGT TCCCTTGAAC ATAATACCTC TTACAACAGC AGCCAAACTA ATGGTTGTCA TACCAGACTA TAACACATAT AAAAATACGT GTGATGGTAC AACATTTACT TATGCATCAG CATTGTGGGA AATCCAACAG GTTGTAGATG CAGATAGTAA AATTGTTCAA CTTAGTGAAA TTAGTATGGA CAATTCACCT AATTTAGCAT GGCCTCTTAT TGTAACAGCT TTAAGGGCCA ATTCTGCTGT CAAATTACAG AATAATGAGC TTAGTCCTGT TGCACTACGA CAGATGTCTT GTGCTGCCGG TACTACACAA ACTGCTTGCA CTGATGACAA TGCGTTAGCT TACTACAACA CAACAAAGGG AGGTAGGTTT GTACTTGCAC TGTTATCCGA TTTACAGGAT TTGAAATGGG CTAGATTCCC TAAGAGTGAT GGAACTGGTA CTATCTATAC AGAACTGGAA CCACCTTGTA GGTTTGTTAC AGACACACCT AAAGGTCCTA AAGTGAAGTA TTTATACTTT ATTAAAGGAT TAAACAACCT AAATAGAGGT ATGGTACTTG GTAGTTTAGC TGCCACAGTA CGTCTACAAG CTGGTAATGC AACAGAAGTG CCTGCCAATT CAACTGTATT ATCTTTCTGT GCTTTTGCTG TAGATGCTGC TAAAGCTTAC AAAGATTATC TAGCTAGTGG GGGACAACCA ATCACTAATT GTGTTAAGAT GTTGTGTACA CACACTGGTA CTGGTCAGGC AATAACAGTT ACACCGGAAG CCAATATGGA TCAAGAATCC TTTGGTGGTG CATCGTGTTG TCTGTACTGC CGTTGCCACA TAGATCATCC AAATCCTAAA GGATTTTGTG ACTTAAAAGG TAAGTATGTA CAAATACCTA CAACTTGTGC TAATGACCCT GTGGGTTTTA CACTTAAAAA CACAGTCTGT ACCGTCTGCG GTATGTGGAA AGGTTATGGC TGTAGTTGTG ATCAACTCCG CGAACCCATG CTTCAGTCAG CTGATGCACA ATCGTTTTTA AACCGGGTTT GCGGTGTAAG TGCAGCCCGT CTTACACCGT GCGGCACAGG CACTAGTACT GATGTCGTAT ACAGGGCTTT TGACATCTAC AATGATAAAG TAGCTGGTTT TGCTAAATTC CTAAAAACTA ATTGTTGTCG CTTCCAAGAA AAGGACGAAG ATGACAATTT AATTGATTCT TACTTTGTAG TTAAGAGACA CACTTTCTCT AACTACCAAC ATGAAGAAAC AATTTATAAT TTACTTAAGG ATTGTCCAGC TGTTGCTAAA CATGACTTCT TTAAGTTTAG AATAGACGGT GACATGGTAC CACATATATC ACGTCAACGT CTTACTAAAT ACACAATGGC AGACCTCGTC TATGCTTTAA GGCATTTTGA TGAAGGTAAT TGTGACACAT TAAAAGAAAT ACTTGTCACA TACAATTGTT GTGATGATGA TTATTTCAAT AAAAAGGACT GGTATGATTT TGTAGAAAAC CCAGATATAT TACGCGTATA CGCCAACTTA GGTGAACGTG TACGCCAAGC TTTGTTAAAA ACAGTACAAT TCTGTGATGC CATGCGAAAT GCTGGTATTG TTGGTGTACT GACATTAGAT AATCAAGATC TCAATGGTAA CTGGTATGAT TTCGGTGATT TCATACAAAC CACGCCAGGT AGTGGAGTTC CTGTTGTAGA TTCTTATTAT TCATTGTTAA TGCCTATATT AACCTTGACC AGGGCTTTAA CTGCAGAGTC ACATGTTGAC ACTGACTTAA CAAAGCCTTA CATTAAGTGG GATTTGTTAA AATATGACTT CACGGAAGAG AGGTTAAAAC TCTTTGACCG TTATTTTAAA TATTGGGATC AGACATACCA CCCAAATTGT GTTAACTGTT TGGATGACAG ATGCATTCTG CATTGTGCAA ACTTTAATGT TTTATTCTCT ACAGTGTTCC CACCTACAAG TTTTGGACCA CTAGTGAGAA AAATATTTGT TGATGGTGTT CCATTTGTAG TTTCAACTGG ATACCACTTC AGAGAGCTAG GTGTTGTACA TAATCAGGAT GTAAACTTAC ATAGCTCTAG ACTTAGTTTT AAGGAATTAC TTGTGTATGC TGCTGACCCT GCTATGCACG CTGCTTCTGG TAATCTATTA CTAGATAAAC GCACTACGTG CTTTTCAGTA GCTGCACTTA CTAACAATGT TGCTTTTCAA ACTGTCAAAC CCGGTAATTT TAACAAAGAC TTCTATGACT TTGCTGTGTC TAAGGGTTTC TTTAAGGAAG GAAGTTCTGT TGAATTAAAA CACTTCTTCT TTGCTCAGGA TGGTAATGCT GCTATCAGCG ATTATGACTA CTATCGTTAT AATCTACCAA CAATGTGTGA TATCAGACAA CTACTATTTG TAGTTGAAGT TGTTGATAAG TACTTTGATT GTTACGATGG TGGCTGTATT AATGCTAACC AAGTCATCGT CAACAACCTA GACAAATCAG CTGGTTTTCC ATTTAATAAA TGGGGTAAGG CTAGACTTTA TTATGATTCA ATGAGTTATG AGGATCAAGA TGCACTTTTC GCATATACAA AACGTAATGT CATCCCTACT ATAACTCAAA TGAATCTTAA GTATGCCATT AGTGCAAAGA ATAGAGCTCG CACCGTAGCT GGTGTCTCTA TCTGTAGTAC TATGACCAAT AGACAGTTTC ATCAAAAATT ATTGAAATCA ATAGCCGCCA CTAGAGGAGC TACTGTAGTA ATTGGAACAA GCAAATTCTA TGGTGGTTGG CACAACATGT TAAAAACTGT TTATAGTGAT GTAGAAAACC CTCACCTTAT GGGTTGGGAT TATCCTAAAT GTGATAGAGC CATGCCTAAC ATGCTTAGAA TTATGGCCTC ACTTGTTCTT GCTCGCAAAC ATACAACGTG TTGTAGCTTG TCACACCGTT TCTATAGATT AGCTAATGAG TGTGCTCAAG TATTGAGTGA AATGGTCATG TGTGGCGGTT CACTATATGT TAAACCAGGT GGAACCTCAT CAGGAGATGC CACAACTGCT TATGCTAATA GTGTTTTTAA CATTTGTCAA GCTGTCACGG CCAATGTTAA TGCACTTTTA TCTACTGATG GTAACAAAAT TGCCGATAAG TATGTCCGCA ATTTACAACA CAGACTTTAT GAGTGTCTCT ATAGAAATAG AGATGTTGAC ACAGACTTTG TGAATGAGTT TTACGCATAT TTGCGTAAAC ATTTCTCAAT GATGATACTC TCTGACGATG CTGTTGTGTG TTTCAATAGC ACTTATGCAT CTCAAGGTCT AGTGGCTAGC ATAAAGAACT TTAAGTCAGT TCTTTATTAT CAAAACAATG TTTTTATGTC TGAAGCAAAA TGTTGGACTG AGACTGACCT TACTAAAGGA CCTCATGAAT TTTGCTCTCA ACATACAATG CTAGTTAAAC AGGGTGATGA TTATGTGTAC CTTCCTTACC CAGATCCATC AAGAATCCTA GGGGCCGGCT GTTTTGTAGA TGATATCGTA AAAACAGATG GTACACTTAT GATTGAACGG TTCGTGTCTT TAGCTATAGA TGCTTACCCA CTTACTAAAC ATCCTAATCA GGAGTATGCT GATGTCTTTC ATTTGTACTT ACAATACATA AGAAAGCTAC ATGATGAGTT AACAGGACAC ATGTTAGACA TGTATTCTGT TATGCTTACT AATGATAACA CTTCAAGGTA TTGGGAACCT GAGTTTTATG AGGCTATGTA CACACCGCAT ACAGTCTTAC AGGCTGTTGG GGCTTGTGTT CTTTGCAATT CACAGACTTC ATTAAGATGT GGTGCTTGCA TACGTAGACC ATTCTTATGT TGTAAATGCT GTTACGACCA TGTCATATCA ACATCACATA AATTAGTCTT GTCTGTTAAT CCGTATGTTT GCAATGCTCC AGGTTGTGAT GTCACAGATG TGACTCAACT TTACTTAGGA GGTATGAGCT ATTATTGTAA ATCACATAAA CCACCCATTA GTTTTCCATT GTGTGCTAAT GGACAAGTTT TTGGTTTATA TAAAAATACA TGTGTTGGTA GCGATAATGT TACTGACTTT AATGCAATTG CAACATGTGA CTGGACAAAT GCTGGTGATT ACATTTTAGC TAACACCTGT ACTGAAAGAC TCAAGCTTTT TGCAGCAGAA ACGCTCAAAG CTACTGAGGA GACATTTAAA CTGTCTTATG GTATTGCTAC TGTACGTGAA GTGCTGTCTG ACAGAGAATT ACATCTTTCA TGGGAAGTTG GTAAACCTAG ACCACCACTT AACCGAAATT ATGTCTTTAC TGGTTATCGT GTAACTAAAA ACAGTAAAGT ACAAATAGGA GAGTACACCT TTGAAAAAGG TGACTATGGT GATGCTGTTG TTTACCGAGG TACAACAACT TACAAATTAA ATGTTGGTGA TTATTTTGTG CTGACATCAC ATACAGTAAT GCCATTAAGT GCACCTACAC TAGTGCCACA AGAGCACTAT GTTAGAATTA CTGGCTTATA CCCAACACTC AATATCTCAG ATGAGTTTTC TAGCAATGTT GCAAATTATC AAAAGGTTGG TATGCAAAAG TATTCTACAC TCCAGGGACC ACCTGGTACT GGTAAGAGTC ATTTTGCTAT TGGCCTAGCT CTCTACTACC CTTCTGCTCG CATAGTGTAT ACAGCTTGCT CTCATGCCGC TGTTGATGCA CTATGTGAGA AGGCATTAAA ATATTTGCCT ATAGATAAAT GTAGTAGAAT TATACCTGCA CGTGCTCGTG TAGAGTGTTT TGATAAATTC AAAGTGAATT CAACATTAGA ACAGTATGTC TTTTGTACTG TAAATGCATT GCCTGAGACG ACAGCAGATA TAGTTGTCTT TGATGAAATT TCAATGGCCA CAAATTATGA TTTGAGTGTT GTCAATGCCA GATTACGTGC TAAGCACTAT GTGTACATTG GCGACCCTGC TCAATTACCT GCACCACGCA CATTGCTAAC TAAGGGCACA CTAGAACCAG AATATTTCAA TTCAGTGTGT AGACTTATGA AAACTATAGG TCCAGACATG TTCCTCGGAA CTTGTCGGCG TTGTCCTGCT GAAATTGTTG ACACTGTGAG TGCTTTGGTT TATGATAATA AGCTTAAAGC ACATAAAGAC AAATCAGCTC AATGCTTTAA AATGTTTTAT AAGGGTGTTA TCACGCATGA TGTTTCATCT GCAATTAACA GGCCACAAAT AGGCGTGGTA AGAGAATTCC TTACACGTAA CCCTGCTTGG AGAAAAGCTG TCTTTATTTC ACCTTATAAT TCACAGAATG CTGTAGCCTC AAAGATTTTG GGACTACCAA CTCAAACTGT TGATTCATCA CAGGGCTCAG AATATGACTA TGTCATATTC ACTCAAACCA CTGAAACAGC TCACTCTTGT AATGTAAACA GATTTAATGT TGCTATTACC AGAGCAAAAG TAGGCATACT TTGCATAATG TCTGATAGAG ACCTTTATGA CAAGTTGCAA TTTACAAGTC TTGAAATTCC ACGTAGGAAT GTGGCAACTT TACAAGCTGA AAATGTAACA GGACTCTTTA AAGATTGTAG TAAGGTAATC ACTGGGTTAC ATCCTACACA GGCACCTACA CACCTCAGTG TTGACACTAA ATTCAAAACT GAAGGTTTAT GTGTTGACAT ACCTGGCATA CCTAAGGACA TGACCTATAG AAGACTCATC TCTATGATGG GTTTTAAAAT GAATTATCAA GTTAATGGTT ACCCTAACAT GTTTATCACC CGCGAAGAAG CTATAAGACA TGTACGTGCA TGGATTGGCT TCGATGTCGA GGGGTGTCAT GCTACTAGAG AAGCTGTTGG TACCAATTTA CCTTTACAGC TAGGTTTTTC TACAGGTGTT AACCTAGTTG CTGTACCTAC AGGTTATGTT GATACACCTA ATAATACAGA TTTTTCCAGA GTTAGTGCTA AACCACCGCC TGGAGATCAA TTTAAACACC TCATACCACT TATGTACAAA GGACTTCCTT GGAATGTAGT GCGTATAAAG ATTGTACAAA TGTTAAGTGA CACACTTAAA AATCTCTCTG ACAGAGTCGT ATTTGTCTTA TGGGCACATG GCTTTGAGTT GACATCTATG AAGTATTTTG TGAAAATAGG ACCTGAGCGC ACCTGTTGTC TATGTGATAG ACGTGCCACA TGCTTTTCCA CTGCTTCAGA CACTTATGCC TGTTGGCATC ATTCTATTGG ATTTGATTAC GTCTATAATC CGTTTATGAT TGATGTTCAA CAATGGGGTT TTACAGGTAA CCTACAAAGC AACCATGATC TGTATTGTCA AGTCCATGGT AATGCACATG TAGCTAGTTG TGATGCAATC ATGACTAGGT GTCTAGCTGT CCACGAGTGC TTTGTTAAGC GTGTTGACTG GACTATTGAA TATCCTATAA TTGGTGATGA ACTGAAGATT AATGCGGCTT GTAGAAAGGT TCAACACATG GTTGTTAAAG CTGCATTATT AGCAGACAAA TTCCCAGTTC TTCACGACAT TGGTAACCCT AAAGCTATTA AGTGTGTACC TCAAGCTGAT GTAGAATGGA AGTTCTATGA TGCACAGCCT TGTAGTGACA AAGCTTATAA AATAGAAGAA TTATTCTATT CTTATGCCAC ACATTCTGAC AAATTCACAG ATGGTGTATG CCTATTTTGG AATTGCAATG TCGATAGATA TCCTGCTAAT TCCATTGTTT GTAGATTTGA CACTAGAGTG CTATCTAACC TTAACTTGCC TGGTTGTGAT GGTGGCAGTT TGTATGTAAA TAAACATGCA TTCCACACAC CAGCTTTTGA TAAAAGTGCT TTTGTTAATT TAAAACAATT ACCATTTTTC TATTACTCTG ACAGTCCATG TGAGTCTCAT GGAAAACAAG TAGTGTCAGA TATAGATTAT GTACCACTAA AGTCTGCTAC GTGTATAACA CGTTGCAATT TAGGTGGTGC TGTCTGTAGA CATCATGCTA ATGAGTACAG ATTGTATCTC GATGCTTATA ACATGATGAT CTCAGCTGGC TTTAGCTTGT GGGTTTACAA ACAATTTGAT ACTTATAACC TCTGGAACAC TTTTACAAGA CTTCAGAGTT TAGAAAATGT GGCTTTTAAT GTTGTAAATA AGGGACACTT TGATGGACAA CAGGGTGAAG TACCAGTTTC TATCATTAAT AACACTGTTT ACACAAAAGT TGATGGTGTT GATGTAGAAT TGTTTGAAAA TAAAACAACA TTACCTGTTA ATGTAGCATT TGAGCTTTGG GCTAAGCGCA ACATTAAACC AGTACCAGAG GTGAAAATAC TCAATAATTT GGGTGTGGAC ATTGCTGCTA ATACTGTGAT CTGGGACTAC AAAAGAGATG CTCCAGCACA TATATCTACT ATTGGTGTTT GTTCTATGAC TGACATAGCC AAGAAACCAA CTGAAACGAT TTGTGCACCA CTCACTGTCT TTTTTGATGG TAGAGTTGAT GGTCAAGTAG ACTTATTTAG AAATGCCCGT AATGGTGTTC TTATTACAGA AGGTAGTGTT AAAGGTTTAC AACCATCTGT AGGTCCCAAA CAAGCTAGTC TTAATGGAGT CACATTAATT GGAGAAGCCG TAAAAACACA GTTCAATTAT TATAAGAAAG TTGATGGTGT TGTCCAACAA TTACCTGAAA CTTACTTTAC TCAGAGTAGA AATTTACAAG AATTTAAACC CAGGAGTCAA ATGGAAATTG ATTTCTTAGA ATTAGCTATG GATGAATTCA TTGAACGGTA TAAATTAGAA GGCTATGCCT TCGAACATAT CGTTTATGGA GATTTTAGTC ATAGTCAGTT AGGTGGTTTA CATCTACTGA TTGGACTAGC TAAACGTTTT AAGGAATCAC CTTTTGAATT AGAAGATTTT ATTCCTATGG ACAGTACAGT TAAAAACTAT TTCATAACAG ATGCGCAAAC AGGTTCATCT AAGTGTGTGT GTTCTGTTAT TGATTTATTA CTTGATGATT TTGTTGAAAT AATAAAATCC CAAGATTTAT CTGTAGTTTC TAAGGTTGTC AAAGTGACTA TTGACTATAC AGAAATTTCA TTTATGCTTT GGTGTAAAGA TGGCCATGTA GAAACATTTT ACCCAAAATT ACAATCTAGT CAAGCGTGGC AACCGGGTGT TGCTATGCCT AATCTTTACA AAATGCAAAG AATGCTATTA GAAAAGTGTG ACCTTCAAAA TTATGGTGAT AGTGCAACAT TACCTAAAGG CATAATGATG AATGTCGCAA AATATACTCA ACTGTGTCAA TATTTAAACA CATTAACATT AGCTGTACCC TATAATATGA GAGTTATACA TTTTGGTGCT GGTTCTGATA AAGGAGTTGC ACCAGGTACA GCTGTTTTAA GACAGTGGTT GCCTACGGGT ACGCTGCTTG TCGATTCAGA TCTTAATGAC TTTGTCTCTG ATGCAGATTC AACTTTGATT GGTGATTGTG CAACTGTACA TACAGCTAAT AAATGGGATC TCATTATTAG TGATATGTAC GACCCTAAGA CTAAAAATGT TACAAAAGAA AATGACTCTA AAGAGGGTTT TTTCACTTAC ATTTGTGGGT TTATACAACA AAAGCTAGCT CTTGGAGGTT CCGTGGCTAT AAAGATAACA GAACATTCTT GGAATGCTGA TCTTTATAAG CTCATGGGAC ACTTCGCATG GTGGACAGCC TTTGTTACTA ATGTGAATGC GTCATCATCT GAAGCATTTT TAATTGGATG TAATTATCTT GGCAAACCAC GCGAACAAAT AGATGGTTAT GTCATGCATG CAAATTACAT ATTTTGGAGG AATACAAATC CAATTCAGTT GTCTTCCTAT TCTTTATTTG ACATGAGTAA ATTTCCCCTT AAATTAAGGG GTACTGCTGT TATGTCTTTA AAAGAAGGTC AAATCAATGA TATGATTTTA TCTCTTCTTA GTAAAGGTAG ACTTATAATT AGAGAAAACA ACAGAGTTGT TATTTCTAGT GATGTTCTTG TTAACAAC
Details for BewuA.18928.a.MX101.PB00110
PURIFICATION DATe: 4/21/2020
CONCENTRATION: 14.4mg/ml
OBSERVED MW: 150kDa
EXPRESSION LEVEL: Data Unavailable
PROTEIN PURIFICATION BUFFER: 150 mM NaCl, 50 NaPO4, 1 mM DTT, pH 6.5
EXPRESSION HOST: Data not available
VIAL COUNT (approx.): 1
VIAL VOLUME: 400µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGENLYFQG HMNNELSPVA LRQMSCAAGT TQTACTDDNA LAYYNTTKGG RFVLALLSDL QDLKWARFPK SDGTGTIYTE LEPPCRFVTD TPKGPKVKYL YFIKGLNNLN RGMVLGSLAA TVRLQ
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCG AGAATCTTTA TTTTCAGGGC CATATGAACA ACGAGCTGAG CCCGGTTGCG CTGCGTCAGA TGAGCTGCGC GGCGGGTACC ACCCAAACCG CGTGCACCGA CGATAACGCG CTGGCGTACT ATAACACCAC CAAGGGTGGC CGTTTCGTTC TGGCGCTGCT GAGCGACCTG CAGGATCTGA AGTGGGCGCG TTTTCCGAAA AGCGACGGTA CCGGCACCAT CTACACCGAG CTGGAACCGC CGTGCCGTTT CGTGACCGAT ACCCCGAAGG GTCCGAAGGT TAAGTACCTG TACTTCATCA AGGGCCTGAA CAACCTGAAC CGTGGTATGG TGCTGGGCAG CCTGGCGGCG ACCGTTCGTC TGCAATAAGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for BewuA.18928.a.MX81.PB00111
PURIFICATION DATe: 4/21/2020
CONCENTRATION: 7.4mg/ml
OBSERVED MW: 119kDa
EXPRESSION LEVEL: Data Unavailable
PROTEIN PURIFICATION BUFFER: 150 mM NaCl, 50 NaPO4, 1 mM DTT, pH 6.5
EXPRESSION HOST: Data not available
VIAL COUNT (approx.): 1
VIAL VOLUME: 500µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGENLYFQG HMSKMSDVKC TSVVLLSVLQ QLRVESSSKL WAQCVQLHND ILLAKDTTEA FEKMVSLLSV LLSMQGAVDI NKLCEEMLDN RATLQ
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCG AGAATCTTTA TTTTCAGGGC CATATGAGCA AGATGAGCGA CGTGAAATGC ACCAGCGTGG TTCTGCTGAG CGTGCTGCAG CAACTGCGTG TTGAGAGCAG CAGCAAGCTG TGGGCGCAGT GCGTTCAACT GCACAACGAC ATCCTGCTGG CGAAGGATAC CACCGAGGCG TTCGAAAAAA TGGTGAGCCT GCTGAGCGTT CTGCTGAGCA TGCAGGGTGC GGTGGACATT AACAAACTGT GCGAGGAAAT GCTGGATAAC CGTGCGACCC TGCAATAAGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for BewuA.18928.a.R91.PB00112
PURIFICATION DATe: 4/21/2020
CONCENTRATION: 23mg/ml
OBSERVED MW: 24kDa
EXPRESSION LEVEL: Data Unavailable
PROTEIN PURIFICATION BUFFER: 150 mM NaCl, 50 NaPO4, 1 mM DTT, pH 6.5
EXPRESSION HOST: Data not available
VIAL COUNT (approx.): 1
VIAL VOLUME: 500µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGLVPRGSH MAIASEFSSL PSYAAFATAQ EAYEQAVANG DSEVVLKKLK KSLNVAKSEF DRDAAMQRKL EKMADQAMTQ MYKQARSEDK RAKVTSAMQT MLFTMLRKLD NDALNNIINN ARDGCVPLNI IPLTTAAKLM VVIPDYNTYK NTCDGTTFTY ASALWEIQQV VDADSKIVQL SEISMDNSPN LAWPLIVTAL RANSAVKLQ
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT GCGATCGCGA GCGAGTTCAG CAGCCTGCCG AGCTACGCGG CGTTTGCGAC CGCGCAAGAG GCGTATGAAC AGGCGGTGGC GAACGGTGAC AGCGAAGTGG TTCTGAAGAA ACTGAAGAAA AGCCTGAACG TTGCGAAGAG CGAGTTCGAC CGTGATGCGG CGATGCAGCG TAAGCTGGAA AAAATGGCGG ACCAGGCGAT GACCCAAATG TACAAGCAGG CGCGTAGCGA GGATAAGCGT GCGAAAGTGA CCAGCGCGAT GCAGACCATG CTGTTTACCA TGCTGCGTAA ACTGGACAAC GATGCGCTGA ACAACATCAT TAACAACGCG CGTGACGGTT GCGTTCCGCT GAACATCATT CCGCTGACCA CCGCGGCGAA GCTGATGGTG GTTATCCCGG ACTACAACAC CTATAAAAAC ACCTGCGATG GCACCACCTT CACCTATGCG AGCGCGCTGT GGGAGATTCA GCAAGTGGTT GACGCGGATA GCAAGATCGT GCAACTGAGC GAAATTAGCA TGGATAACAG CCCGAACCTG GCGTGGCCGC TGATTGTGAC CGCGCTGCGT GCGAACAGCG CGGTTAAACT GCAGTAACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for BewuA.18928.a.MX81.PS38718
PURIFICATION DATe: 3/24/2023
CONCENTRATION: 4.727mg/ml
OBSERVED MW: 12kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: Rosetta2 (DE3)
VIAL COUNT (approx.): 19
VIAL VOLUME: 500µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGENLYFQG HMSKMSDVKC TSVVLLSVLQ QLRVESSSKL WAQCVQLHND ILLAKDTTEA FEKMVSLLSV LLSMQGAVDI NKLCEEMLDN RATLQ
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCG AGAATCTTTA TTTTCAGGGC CATATGAGCA AGATGAGCGA CGTGAAATGC ACCAGCGTGG TTCTGCTGAG CGTGCTGCAG CAACTGCGTG TTGAGAGCAG CAGCAAGCTG TGGGCGCAGT GCGTTCAACT GCACAACGAC ATCCTGCTGG CGAAGGATAC CACCGAGGCG TTCGAAAAAA TGGTGAGCCT GCTGAGCGTT CTGCTGAGCA TGCAGGGTGC GGTGGACATT AACAAACTGT GCGAGGAAAT GCTGGATAAC CGTGCGACCC TGCAATAAGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for BewuA.18928.a.MX111.PS38719
PURIFICATION DATe: 3/31/2023
CONCENTRATION: 5.55mg/ml
OBSERVED MW: 18kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: Rosetta2 (DE3)
VIAL COUNT (approx.): 10
VIAL VOLUME: 500µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGENLYFQG HMAGNATEVP ANSTVLSFCA FAVDAAKAYK DYLASGGQPI TNCVKMLCTH TGTGQAITVT PEANMDQESF GGASCCLYCR CHIDHPNPKG FCDLKGKYVQ IPTTCANDPV GFTLKNTVCT VCGMWKGYGC SCDQLREPML Q
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCG AGAATCTTTA TTTTCAGGGC CATATGGCGG GTAATGCGAC CGAAGTTCCG GCGAATAGCA CCGTTCTGAG CTTTTGCGCG TTTGCGGTTG ATGCGGCGAA GGCGTACAAG GATTACCTGG CGAGCGGTGG CCAGCCGATC ACCAACTGCG TGAAGATGCT GTGCACCCAC ACCGGTACCG GTCAGGCGAT TACCGTTACC CCGGAGGCGA ACATGGACCA AGAAAGCTTT GGTGGCGCGA GCTGCTGCCT GTACTGCCGT TGCCACATCG ACCACCCGAA CCCGAAGGGT TTTTGCGATC TGAAGGGCAA ATATGTGCAA ATTCCGACCA CCTGCGCGAA CGATCCGGTT GGTTTCACCC TGAAAAACAC CGTGTGCACC GTTTGCGGTA TGTGGAAGGG TTACGGTTGC AGCTGCGACC AACTGCGTGA GCCGATGCTG CAATAAGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT
Details for BewuA.18928.a.R91.PW38804
PURIFICATION DATe: 3/5/2020
CONCENTRATION: 23mg/ml
OBSERVED MW: 24kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: Data not available
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGLVPRGSH MAIASEFSSL PSYAAFATAQ EAYEQAVANG DSEVVLKKLK KSLNVAKSEF DRDAAMQRKL EKMADQAMTQ MYKQARSEDK RAKVTSAMQT MLFTMLRKLD NDALNNIINN ARDGCVPLNI IPLTTAAKLM VVIPDYNTYK NTCDGTTFTY ASALWEIQQV VDADSKIVQL SEISMDNSPN LAWPLIVTAL RANSAVKLQ
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT GCGATCGCGA GCGAGTTCAG CAGCCTGCCG AGCTACGCGG CGTTTGCGAC CGCGCAAGAG GCGTATGAAC AGGCGGTGGC GAACGGTGAC AGCGAAGTGG TTCTGAAGAA ACTGAAGAAA AGCCTGAACG TTGCGAAGAG CGAGTTCGAC CGTGATGCGG CGATGCAGCG TAAGCTGGAA AAAATGGCGG ACCAGGCGAT GACCCAAATG TACAAGCAGG CGCGTAGCGA GGATAAGCGT GCGAAAGTGA CCAGCGCGAT GCAGACCATG CTGTTTACCA TGCTGCGTAA ACTGGACAAC GATGCGCTGA ACAACATCAT TAACAACGCG CGTGACGGTT GCGTTCCGCT GAACATCATT CCGCTGACCA CCGCGGCGAA GCTGATGGTG GTTATCCCGG ACTACAACAC CTATAAAAAC ACCTGCGATG GCACCACCTT CACCTATGCG AGCGCGCTGT GGGAGATTCA GCAAGTGGTT GACGCGGATA GCAAGATCGT GCAACTGAGC GAAATTAGCA TGGATAACAG CCCGAACCTG GCGTGGCCGC TGATTGTGAC CGCGCTGCGT GCGAACAGCG CGGTTAAACT GCAGTAACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for BewuA.18928.a.MX101.PW38805
PURIFICATION DATe: 3/5/2020
CONCENTRATION: 34mg/ml
OBSERVED MW: 15kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: Data not available
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGENLYFQG HMNNELSPVA LRQMSCAAGT TQTACTDDNA LAYYNTTKGG RFVLALLSDL QDLKWARFPK SDGTGTIYTE LEPPCRFVTD TPKGPKVKYL YFIKGLNNLN RGMVLGSLAA TVRLQ
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCG AGAATCTTTA TTTTCAGGGC CATATGAACA ACGAGCTGAG CCCGGTTGCG CTGCGTCAGA TGAGCTGCGC GGCGGGTACC ACCCAAACCG CGTGCACCGA CGATAACGCG CTGGCGTACT ATAACACCAC CAAGGGTGGC CGTTTCGTTC TGGCGCTGCT GAGCGACCTG CAGGATCTGA AGTGGGCGCG TTTTCCGAAA AGCGACGGTA CCGGCACCAT CTACACCGAG CTGGAACCGC CGTGCCGTTT CGTGACCGAT ACCCCGAAGG GTCCGAAGGT TAAGTACCTG TACTTCATCA AGGGCCTGAA CAACCTGAAC CGTGGTATGG TGCTGGGCAG CCTGGCGGCG ACCGTTCGTC TGCAATAAGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for BewuA.18928.a.MX161.PW38807
PURIFICATION DATe: 3/4/2020
CONCENTRATION: 19mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: Data not available
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGENLYFQG HMSSQAWQPG VAMPNLYKMQ RMLLEKCDLQ NYGDSATLPK GIMMNVAKYT QLCQYLNTLT LAVPYNMRVI HFGAGSDKGV APGTAVLRQW LPTGTLLVDS DLNDFVSDAD STLIGDCATV HTANKWDLII SDMYDPKTKN VTKENDSKEG FFTYICGFIQ QKLALGGSVA IKITEHSWNA DLYKLMGHFA WWTAFVTNVN ASSSEAFLIG CNYLGKPREQ IDGYVMHANY IFWRNTNPIQ LSSYSLFDMS KFPLKLRGTA VMSLKEGQIN DMILSLLSKG RLIIRENNRV VISSDVLVNN
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCG AGAATCTTTA TTTTCAGGGC CATATGAGCA GCCAAGCGTG GCAACCGGGT GTGGCGATGC CGAACCTGTA TAAAATGCAA CGTATGCTGC TGGAGAAATG CGACCTGCAA AACTACGGTG ACAGCGCGAC CCTGCCGAAG GGCATCATGA TGAACGTGGC GAAATACACC CAGCTGTGCC AATATCTGAA CACCCTGACC CTGGCGGTGC CGTATAACAT GCGTGTTATT CACTTCGGTG CGGGTAGCGA CAAGGGTGTG GCGCCGGGTA CCGCGGTTCT GCGTCAGTGG CTGCCGACCG GTACCCTGCT GGTTGACAGC GATCTGAACG ATTTTGTTAG CGACGCGGAT AGCACCCTGA TTGGTGACTG CGCGACCGTG CACACCGCGA ACAAATGGGA TCTGATCATT AGCGACATGT ACGATCCGAA GACCAAAAAC GTTACCAAGG AGAACGACAG CAAAGAAGGT TTCTTTACCT ATATCTGCGG CTTCATTCAG CAAAAGCTGG CGCTGGGTGG CAGCGTTGCG ATCAAAATTA CCGAGCACAG CTGGAACGCG GATCTGTACA AGCTGATGGG TCACTTCGCG TGGTGGACCG CGTTTGTGAC CAACGTTAAC GCGAGCAGCA GCGAGGCGTT CCTGATCGGT TGCAACTATC TGGGCAAACC GCGTGAACAG ATTGATGGCT ACGTGATGCA CGCGAACTAT ATCTTTTGGC GTAACACCAA CCCGATTCAA CTGAGCAGCT ACAGCCTGTT CGACATGAGC AAGTTTCCGC TGAAACTGCG TGGTACCGCG GTTATGAGCC TGAAGGAAGG CCAAATCAAC GATATGATTC TGAGCCTGCT GAGCAAAGGT CGTCTGATTA TCCGTGAAAA CAACCGTGTG GTGATTAGCA GCGACGTTCT GGTGAACAAC TAAGCGGCCG CACTCGAGCA CCACCACCAC CACCACTGAG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGAT
Details for BewuA.18928.a.MX111.PW38819
PURIFICATION DATe: 4/27/2020
CONCENTRATION: 9.4mg/ml
OBSERVED MW: 17kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: Data not available
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
TEV cleaved
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGSSHHHHHH SSGENLYFQG HMAGNATEVP ANSTVLSFCA FAVDAAKAYK DYLASGGQPI TNCVKMLCTH TGTGQAITVT PEANMDQESF GGASCCLYCR CHIDHPNPKG FCDLKGKYVQ IPTTCANDPV GFTLKNTVCT VCGMWKGYGC SCDQLREPML Q
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCG AGAATCTTTA TTTTCAGGGC CATATGGCGG GTAATGCGAC CGAAGTTCCG GCGAATAGCA CCGTTCTGAG CTTTTGCGCG TTTGCGGTTG ATGCGGCGAA GGCGTACAAG GATTACCTGG CGAGCGGTGG CCAGCCGATC ACCAACTGCG TGAAGATGCT GTGCACCCAC ACCGGTACCG GTCAGGCGAT TACCGTTACC CCGGAGGCGA ACATGGACCA AGAAAGCTTT GGTGGCGCGA GCTGCTGCCT GTACTGCCGT TGCCACATCG ACCACCCGAA CCCGAAGGGT TTTTGCGATC TGAAGGGCAA ATATGTGCAA ATTCCGACCA CCTGCGCGAA CGATCCGGTT GGTTTCACCC TGAAAAACAC CGTGTGCACC GTTTGCGGTA TGTGGAAGGG TTACGGTTGC AGCTGCGACC AACTGCGTGA GCCGATGCTG CAATAAGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT
Details for BewuA.18928.a.A142.PW38844
PURIFICATION DATe: 6/2/2020
CONCENTRATION: 27mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
try rGTP, AdoMet, and Sinefungin co-crystallization
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SVKRVDWTIE YPIIGDELKI NAACRKVQHM VVKAALLADK FPVLHDIGNP KAIKCVPQAD VEWKFYDAQP CSDKAYKIEE LFYSYATHSD KFTDGVCLFW NCNVDRYPAN SIVCRFDTRV LSNLNLPGCD GGSLYVNKHA FHTPAFDKSA FVNLKQLPFF YYSDSPCESH GKQVVSDIDY VPLKSATCIT RCNLGGAVCR HHANEYRLYL DAYNMMISAG FSLWVYKQFD TYNLWNTFTR LQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGT GAAACGTGTT GACTGGACCA TCGAGTACCC GATCATTGGT GATGAACTGA AGATTAACGC GGCGTGCCGT AAAGTGCAGC ACATGGTGGT TAAGGCGGCG CTGCTGGCGG ACAAATTTCC GGTTCTGCAC GACATCGGCA ACCCGAAGGC GATTAAATGC GTGCCGCAGG CGGATGTTGA ATGGAAATTT TACGACGCGC AACCGTGCAG CGATAAGGCG TATAAAATCG AGGAACTGTT CTACAGCTAT GCGACCCACA GCGACAAGTT TACCGATGGT GTGTGCCTGT TCTGGAACTG CAACGTTGAC CGTTACCCGG CGAACAGCAT TGTGTGCCGT TTCGATACCC GTGTTCTGAG CAACCTGAAC CTGCCGGGTT GCGATGGTGG CAGCCTGTAT GTGAACAAGC ACGCGTTCCA CACCCCGGCG TTTGATAAGA GCGCGTTCGT TAACCTGAAA CAGCTGCCGT TCTTTTACTA TAGCGACAGC CCGTGCGAGA GCCACGGTAA ACAAGTGGTT AGCGACATCG ATTACGTGCC GCTGAAGAGC GCGACCTGCA TTACCCGTTG CAACCTGGGT GGCGCGGTTT GCCGTCACCA CGCGAACGAA TACCGTCTGT ATCTGGACGC GTATAACATG ATGATCAGCG CGGGTTTCAG CCTGTGGGTT TACAAGCAAT TTGATACCTA CAATCTGTGG AACACCTTCA CCCGCCTGCA ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA