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Target Status
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Betacoronavirus
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BewuA.18655.a

BewuA.18655.a: Spike glycoprotein (S)

Target Characteristics

from organism: Betacoronavirus SARS-CoV-2 (2019-nCoV) (COVID-19) isolate Wuhan-Hu-1
most recent status: in PDB
center reference id: BewuA.18655.a
is community request: True
associated disease:
NIH risk group: 2
is select agent: False
NIH priority
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Structures

image of structure for 6VXX
6VXX
Deposited: 2/25/2020
Determination: CryoEM
Clone: BewuA.18655.a.MG1.GE43647
Protein: BewuA.18655.a.MG1.PE00013
image of structure for 6VYB
6VYB
Deposited: 2/25/2020
Determination: CryoEM
Clone: BewuA.18655.a.MG1.GE43647
Protein: BewuA.18655.a.MG1.PE00013
image of structure for 6WPS
6WPS
Deposited: 4/27/2020
Determination: CryoEM
Clone: BewuA.18655.a.K14.GE43854
Protein: BewuA.18655.a.K14.PE00015
image of structure for 6WPT
6WPT
Deposited: 4/27/2020
Determination: CryoEM
Clone: BewuA.18655.a.K14.GE43854
Protein: BewuA.18655.a.K14.PE00015
image of structure for 6X79
6X79
Deposited: 5/29/2020
Determination: CryoEM
Clone: BewuA.18655.a.TZ31.GE43861
Protein: BewuA.18655.a.TZ31.PE00016
image of structure for 7K4N
7K4N
Deposited: 9/15/2020
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00017
image of structure for 7K45
7K45
Deposited: 9/14/2020
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00017
image of structure for 7JZL
7JZL
Deposited: 9/2/2020
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00018
image of structure for 7JZU
7JZU
Deposited: 9/2/2020
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00018
image of structure for 7JZM
7JZM
Deposited: 9/2/2020
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00019
image of structure for 7JZN
7JZN
Deposited: 9/2/2020
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00019
image of structure for 7JX3
7JX3
Deposited: 8/26/2020
Determination: XRay
Diffraction Image
Clone: BewuA.18655.a.K2.GE44140
Protein: BewuA.18655.a.K2.PE00020
image of structure for 7K43
7K43
Deposited: 9/14/2020
Determination: CryoEM
Clone: BewuA.18655.a.PK31.GE44142
Protein: BewuA.18655.a.PK31.PE00021
image of structure for 7JVA
7JVA
Deposited: 8/20/2020
Determination: CryoEM
Clone: BewuA.18655.a.PK31.GE44142
Protein: BewuA.18655.a.PK31.PS38661
image of structure for 7JVC
7JVC
Deposited: 8/20/2020
Determination: CryoEM
Clone: BewuA.18655.a.PK31.GE44142
Protein: BewuA.18655.a.PK31.PS38661
image of structure for 7JV6
7JV6
Deposited: 8/20/2020
Determination: CryoEM
Clone: BewuA.18655.a.PK31.GE44142
Protein: BewuA.18655.a.PK31.PE00022
image of structure for 7JV4
7JV4
Deposited: 8/20/2020
Determination: CryoEM
Clone: BewuA.18655.a.PK31.GE44142
Protein: BewuA.18655.a.PK31.PE00022
image of structure for 7JV2
7JV2
Deposited: 8/20/2020
Determination: CryoEM
Clone: BewuA.18655.a.PK31.GE44142
Protein: BewuA.18655.a.PK31.PE00022
image of structure for 7JW0
7JW0
Deposited: 8/24/2020
Determination: CryoEM
Clone: BewuA.18655.a.PK31.GE44142
Protein: BewuA.18655.a.PK31.PE00023
image of structure for 7LXW
7LXW
Deposited: 3/5/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00033
image of structure for 7LXY
7LXY
Deposited: 3/5/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00034
image of structure for 7LXX
7LXX
Deposited: 3/5/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00035
image of structure for 7LXZ
7LXZ
Deposited: 3/5/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00036
image of structure for 7LY0
7LY0
Deposited: 3/5/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00037
image of structure for 7LY2
7LY2
Deposited: 3/5/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44141
Protein: BewuA.18655.a.PJ41.PE00038
image of structure for 7LY3
7LY3
Deposited: 3/5/2021
Determination: XRay
Diffraction Image
Clone: BewuA.18655.a.K41.GE44378
Protein: BewuA.18655.a.K41.PE00039
image of structure for 7RA8
7RA8
Deposited: 6/30/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44489
Protein: BewuA.18655.a.PJ41.PE00044
image of structure for 7RAL
7RAL
Deposited: 7/1/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44489
Protein: BewuA.18655.a.PJ41.PE00044
image of structure for 7N8H
7N8H
Deposited: 6/14/2021
Determination: CryoEM
Clone: BewuA.18655.a.UP41.GE44484
Protein: BewuA.18655.a.UP41.PE00042
image of structure for 7N8I
7N8I
Deposited: 6/14/2021
Determination: CryoEM
Clone: BewuA.18655.a.UP41.GE44484
Protein: BewuA.18655.a.UP41.PE00042
image of structure for 7R7N
7R7N
Deposited: 6/25/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44481
Protein: BewuA.18655.a.PJ41.PE00043
image of structure for 7TAS
7TAS
Deposited: 12/21/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44703
Protein: BewuA.18655.a.PJ41.PE00050
image of structure for 7TAT
7TAT
Deposited: 12/21/2021
Determination: CryoEM
Clone: BewuA.18655.a.PJ41.GE44703
Protein: BewuA.18655.a.PJ41.PE00050
image of structure for 8DF5
8DF5
Deposited: 6/21/2022
Determination: XRay
Diffraction Image
Clone: BewuA.18655.a.K2.GE44140
Protein: BewuA.18655.a.K2.PE00060
image of structure for 7SKZ
7SKZ
Deposited: 10/22/2021
Determination: XRay
Diffraction Image
Clone: BewuA.18655.a.K51.GE44809
Protein: BewuA.18655.a.K51.PE00061
image of structure for 7SL5
7SL5
Deposited: 10/22/2021
Determination: XRay
Diffraction Image
Clone: BewuA.18655.a.K52.GE44810
Protein: BewuA.18655.a.K52.PE00062
image of structure for 8DYA
8DYA
Deposited: 8/3/2022
Determination: CryoEM
Clone: BewuA.18655.a.UP42.GE44911
Protein: BewuA.18655.a.UP42.PS38708

Molecular models

Method Results
comparative modelling Robetta_15652

Publications by SSGCID

Structure, function and antigenicity of the SARS-CoV-2 spike glycoprotein
Wall A, Tortorici MA, Walls AC, McGuire AT, Veesler D, Park YJ
Cell - 2020
volume 181, issue 2, pages 281-292.e6
PMID: 32155444; PMCID: PMC7102599
PDB: 6VXX, 6VYB
Structure-guided covalent stabilization of coronavirus spike glycoprotein trimers in the closed conformation
Walls AC, Corti D, McCallum M, Bowen J
Nat. Struct. Mol. Biol. - 2020
volume 27, issue 10, pages 942-949
PMID: 32753755; PMCID: PMC7541350
PDB: 6VXX, 6VYB
Elicitation of potent neutralizing antibody responses by designed protein nanoparticle vaccines for SARS-CoV-2
Chen C, Walls AC, Fiala B, Hodge E, Carter L, Lee KK, King NP, Ravichandran R, Chu H, Schafer A, Wrenn S, Pham M, Murphy M, Tse L, Shehata L, O'Connor M, Navarro M, Miranda M, Kraft J, Ogohara C, Palser A, Chalk S, Lee E, Kepl E, Chow C, Sydeman C, Brown B, Fuller J, Dinnon K, Gralinski L, Leist S, Gully K, Lewis T, Guttman M, Fuller D, Baric R, Kellam P, Pepper M, Sheahan T
Cell - 2020
PMID: 32817941; PMCID: PMC7430571
PDB: 6VYB
Shifting mutational constraints in the SARS-CoV-2 receptor-binding domain during viral evolution
Veesler D, Corti D, Snell G, McCallum M, Bloom J, Loes A, Starr T, Greaney A, Dillen J, Hannon WW, Hauser K, Ferri E, Farrel AG, Dadonaite B, Matreyek KA
Science - 2022
PMID: 35762884; PMCID: PMC9273037
PDB: 8DF5
ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies
Walls AC, Veesler D, Lanzavecchia A, Corti D, Sallusto F, Tortorici MA, Pinto D, Bianchi S, Snell G, McCallum M, Bowen J, Sprugasci N, Mele F, Ceschi A, Ferrari P, Garzoni C, Benigni F, Abdelnabi R, Neyts J, Low JS, Noack J, Jerak J, Cassotta A, Montiel_Ruiz M, Joshi A, Stewart C, Foglierini M, Weynand B, Rexhepaj M, Jarrossay D, Morone D, Paparoditis P, Purcell LA
Science - 2022
PMID: 35857703
PDB: 7SKZ, 7SL5
SARS-CoV-2 spike conformation determines plasma neutralizing activity elicited by a wide panel of human vaccines
van Voorhis W, Zhang Z, Tortorici MA, Walls AC, Veesler D, Park YJ, Corti D, Sallusto F, Chu H, Snell G, McCallum M, Bowen J, Sprouse K, Low JS, Jerak J, Franko N, Logue J, Joshi A, Stewart C, Brown JT, Sharkey WK, Mazzitelli IG, Ahmed K, Gori A, Bandera A, Posavad CM, Dan JM, Weiskopf D, Sette A, Crotty S, Iqbal NT, Geffner J, Grifantini R, Nguyen AW, Silva RP, Huang Y, Tiles SW, Shariq A, Maynard JA, Simmerling C
- 2022
volume 78, issue 23 Dec 2022,
PMID: 36356052; PMCID: PMC9765460
PDB: 8DYA

External Resources

Resource Reference ID
GenBank: QHD43416
UniProt: P0DTC2
ViPR: link

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MFVFLVLLPL VSSQCVNLTT RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TQDLFLPFFS NVTWFHAIHV SGTNGTKRFD NPVLPFNDGV YFASTEKSNI IRGWIFGTTL DSKTQSLLIV NNATNVVIKV CEFQFCNDPF LGVYYHKNNK SWMESEFRVY SSANNCTFEY VSQPFLMDLE GKQGNFKNLR EFVFKNIDGY FKIYSKHTPI NLVRDLPQGF SALEPLVDLP IGINITRFQT LLALHRSYLT PGDSSSGWTA GAAAYYVGYL QPRTFLLKYN ENGTITDAVD CALDPLSETK CTLKSFTVEK GIYQTSNFRV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YLYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN FNFNGLTGTG VLTESNKKFL PFQQFGRDIA DTTDAVRDPQ TLEILDITPC SFGGVSVITP GTNTSNQVAV LYQDVNCTEV PVAIHADQLT PTWRVYSTGS NVFQTRAGCL IGAEHVNNSY ECDIPIGAGI CASYQTQTNS PRRARSVASQ SIIAYTMSLG AENSVAYSNN SIAIPTNFTI SVTTEILPVS MTKTSVDCTM YICGDSTECS NLLLQYGSFC TQLNRALTGI AVEQDKNTQE VFAQVKQIYK TPPIKDFGGF NFSQILPDPS KPSKRSFIED LLFNKVTLAD AGFIKQYGDC LGDIAARDLI CAQKFNGLTV LPPLLTDEMI AQYTSALLAG TITSGWTFGA GAALQIPFAM QMAYRFNGIG VTQNVLYENQ KLIANQFNSA IGKIQDSLSS TASALGKLQD VVNQNAQALN TLVKQLSSNF GAISSVLNDI LSRLDKVEAE VQIDRLITGR LQSLQTYVTQ QLIRAAEIRA SANLAATKMS ECVLGQSKRV DFCGKGYHLM SFPQSAPHGV VFLHVTYVPA QEKNFTTAPA ICHDGKAHFP REGVFVSNGT HWFVTQRNFY EPQIITTDNT FVSGNCDVVI GIVNNTVYDP LQPELDSFKE ELDKYFKNHT SPDVDLGDIS GINASVVNIQ KEIDRLNEVA KNLNESLIDL QELGKYEQYI KWPWYIWLGF IAGLIAIVMV TIMLCCMTSC CSCLKGCCSC GSCCKFDEDD SEPVLKGVKL HYT

NT Sequence
ATGTTTGTTT TTCTTGTTTT ATTGCCACTA GTCTCTAGTC AGTGTGTTAA TCTTACAACC AGAACTCAAT TACCCCCTGC ATACACTAAT TCTTTCACAC GTGGTGTTTA TTACCCTGAC AAAGTTTTCA GATCCTCAGT TTTACATTCA ACTCAGGACT TGTTCTTACC TTTCTTTTCC AATGTTACTT GGTTCCATGC TATACATGTC TCTGGGACCA ATGGTACTAA GAGGTTTGAT AACCCTGTCC TACCATTTAA TGATGGTGTT TATTTTGCTT CCACTGAGAA GTCTAACATA ATAAGAGGCT GGATTTTTGG TACTACTTTA GATTCGAAGA CCCAGTCCCT ACTTATTGTT AATAACGCTA CTAATGTTGT TATTAAAGTC TGTGAATTTC AATTTTGTAA TGATCCATTT TTGGGTGTTT ATTACCACAA AAACAACAAA AGTTGGATGG AAAGTGAGTT CAGAGTTTAT TCTAGTGCGA ATAATTGCAC TTTTGAATAT GTCTCTCAGC CTTTTCTTAT GGACCTTGAA GGAAAACAGG GTAATTTCAA AAATCTTAGG GAATTTGTGT TTAAGAATAT TGATGGTTAT TTTAAAATAT ATTCTAAGCA CACGCCTATT AATTTAGTGC GTGATCTCCC TCAGGGTTTT TCGGCTTTAG AACCATTGGT AGATTTGCCA ATAGGTATTA ACATCACTAG GTTTCAAACT TTACTTGCTT TACATAGAAG TTATTTGACT CCTGGTGATT CTTCTTCAGG TTGGACAGCT GGTGCTGCAG CTTATTATGT GGGTTATCTT CAACCTAGGA CTTTTCTATT AAAATATAAT GAAAATGGAA CCATTACAGA TGCTGTAGAC TGTGCACTTG ACCCTCTCTC AGAAACAAAG TGTACGTTGA AATCCTTCAC TGTAGAAAAA GGAATCTATC AAACTTCTAA CTTTAGAGTC CAACCAACAG AATCTATTGT TAGATTTCCT AATATTACAA ACTTGTGCCC TTTTGGTGAA GTTTTTAACG CCACCAGATT TGCATCTGTT TATGCTTGGA ACAGGAAGAG AATCAGCAAC TGTGTTGCTG ATTATTCTGT CCTATATAAT TCCGCATCAT TTTCCACTTT TAAGTGTTAT GGAGTGTCTC CTACTAAATT AAATGATCTC TGCTTTACTA ATGTCTATGC AGATTCATTT GTAATTAGAG GTGATGAAGT CAGACAAATC GCTCCAGGGC AAACTGGAAA GATTGCTGAT TATAATTATA AATTACCAGA TGATTTTACA GGCTGCGTTA TAGCTTGGAA TTCTAACAAT CTTGATTCTA AGGTTGGTGG TAATTATAAT TACCTGTATA GATTGTTTAG GAAGTCTAAT CTCAAACCTT TTGAGAGAGA TATTTCAACT GAAATCTATC AGGCCGGTAG CACACCTTGT AATGGTGTTG AAGGTTTTAA TTGTTACTTT CCTTTACAAT CATATGGTTT CCAACCCACT AATGGTGTTG GTTACCAACC ATACAGAGTA GTAGTACTTT CTTTTGAACT TCTACATGCA CCAGCAACTG TTTGTGGACC TAAAAAGTCT ACTAATTTGG TTAAAAACAA ATGTGTCAAT TTCAACTTCA ATGGTTTAAC AGGCACAGGT GTTCTTACTG AGTCTAACAA AAAGTTTCTG CCTTTCCAAC AATTTGGCAG AGACATTGCT GACACTACTG ATGCTGTCCG TGATCCACAG ACACTTGAGA TTCTTGACAT TACACCATGT TCTTTTGGTG GTGTCAGTGT TATAACACCA GGAACAAATA CTTCTAACCA GGTTGCTGTT CTTTATCAGG ATGTTAACTG CACAGAAGTC CCTGTTGCTA TTCATGCAGA TCAACTTACT CCTACTTGGC GTGTTTATTC TACAGGTTCT AATGTTTTTC AAACACGTGC AGGCTGTTTA ATAGGGGCTG AACATGTCAA CAACTCATAT GAGTGTGACA TACCCATTGG TGCAGGTATA TGCGCTAGTT ATCAGACTCA GACTAATTCT CCTCGGCGGG CACGTAGTGT AGCTAGTCAA TCCATCATTG CCTACACTAT GTCACTTGGT GCAGAAAATT CAGTTGCTTA CTCTAATAAC TCTATTGCCA TACCCACAAA TTTTACTATT AGTGTTACCA CAGAAATTCT ACCAGTGTCT ATGACCAAGA CATCAGTAGA TTGTACAATG TACATTTGTG GTGATTCAAC TGAATGCAGC AATCTTTTGT TGCAATATGG CAGTTTTTGT ACACAATTAA ACCGTGCTTT AACTGGAATA GCTGTTGAAC AAGACAAAAA CACCCAAGAA GTTTTTGCAC AAGTCAAACA AATTTACAAA ACACCACCAA TTAAAGATTT TGGTGGTTTT AATTTTTCAC AAATATTACC AGATCCATCA AAACCAAGCA AGAGGTCATT TATTGAAGAT CTACTTTTCA ACAAAGTGAC ACTTGCAGAT GCTGGCTTCA TCAAACAATA TGGTGATTGC CTTGGTGATA TTGCTGCTAG AGACCTCATT TGTGCACAAA AGTTTAACGG CCTTACTGTT TTGCCACCTT TGCTCACAGA TGAAATGATT GCTCAATACA CTTCTGCACT GTTAGCGGGT ACAATCACTT CTGGTTGGAC CTTTGGTGCA GGTGCTGCAT TACAAATACC ATTTGCTATG CAAATGGCTT ATAGGTTTAA TGGTATTGGA GTTACACAGA ATGTTCTCTA TGAGAACCAA AAATTGATTG CCAACCAATT TAATAGTGCT ATTGGCAAAA TTCAAGACTC ACTTTCTTCC ACAGCAAGTG CACTTGGAAA ACTTCAAGAT GTGGTCAACC AAAATGCACA AGCTTTAAAC ACGCTTGTTA AACAACTTAG CTCCAATTTT GGTGCAATTT CAAGTGTTTT AAATGATATC CTTTCACGTC TTGACAAAGT TGAGGCTGAA GTGCAAATTG ATAGGTTGAT CACAGGCAGA CTTCAAAGTT TGCAGACATA TGTGACTCAA CAATTAATTA GAGCTGCAGA AATCAGAGCT TCTGCTAATC TTGCTGCTAC TAAAATGTCA GAGTGTGTAC TTGGACAATC AAAAAGAGTT GATTTTTGTG GAAAGGGCTA TCATCTTATG TCCTTCCCTC AGTCAGCACC TCATGGTGTA GTCTTCTTGC ATGTGACTTA TGTCCCTGCA CAAGAAAAGA ACTTCACAAC TGCTCCTGCC ATTTGTCATG ATGGAAAAGC ACACTTTCCT CGTGAAGGTG TCTTTGTTTC AAATGGCACA CACTGGTTTG TAACACAAAG GAATTTTTAT GAACCACAAA TCATTACTAC AGACAACACA TTTGTGTCTG GTAACTGTGA TGTTGTAATA GGAATTGTCA ACAACACAGT TTATGATCCT TTGCAACCTG AATTAGACTC ATTCAAGGAG GAGTTAGATA AATATTTTAA GAATCATACA TCACCAGATG TTGATTTAGG TGACATCTCT GGCATTAATG CTTCAGTTGT AAACATTCAA AAAGAAATTG ACCGCCTCAA TGAGGTTGCC AAGAATTTAA ATGAATCTCT CATCGATCTC CAAGAACTTG GAAAGTATGA GCAGTATATA AAATGGCCAT GGTACATTTG GCTAGGTTTT ATAGCTGGCT TGATTGCCAT AGTAATGGTG ACAATTATGC TTTGCTGTAT GACCAGTTGC TGTAGTTGTC TCAAGGGCTG TTGTTCTTGT GGATCCTGCT GCAAATTTGA TGAAGACGAC TCTGAGCCAG TGCTCAAAGG AGTCAAATTA CATTACACA

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