BecoA.18657.a

ORF1ab nsp10

CENTER ID: BecoA.18657.a
ORGANISM: Betacoronavirus Human betacoronavirus 2c EMC/2012
ASSOCIATED DISEASE: respiratory, GI infections
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BecoA.18657.a.DG14.GE00191 Full length( BecoA.18657.a ) 9 136
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BecoA.18657.a.DG14.PD00375 Full length( BecoA.18657.a ) 9 136
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|1235996.21.mat_peptide.10
UniProt: K0BWD0
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
AGSNTEFASN SSVLSLVNFT VDPQKAYLDF VNAGGAPLTN CVKMLTPKTG TGIAISVKPE STADQETYGG ASVCLYCRAH IEHPDVSGVC KYKGKFVQIP AQCVRDPVGF CLSNTPCNVC QYWIGYGCNC DSLRQAALPQ
NT Sequence
GCTGGTTCTA ACACCGAGTT TGCCTCTAAT TCCTCGGTGT TGTCACTTGT TAACTTCACC GTTGATCCTC AAAAAGCTTA TCTCGATTTC GTCAATGCGG GAGGTGCCCC ATTGACAAAT TGTGTTAAGA TGCTTACTCC TAAAACTGGT ACAGGTATAG CTATATCTGT TAAACCAGAG AGTACAGCTG ATCAAGAGAC TTATGGTGGA GCTTCAGTGT GTCTCTATTG CCGTGCGCAT ATAGAACATC CTGATGTCTC TGGTGTTTGT AAATATAAGG GTAAGTTTGT CCAAATCCCT GCTCAGTGTG TCCGTGACCC TGTGGGATTT TGTTTGTCAA ATACCCCCTG TAATGTCTGT CAATATTGGA TTGGATATGG GTGCAATTGT GACTCGCTTA GGCAAGCAGC ACTGCCCCAA
Details for BecoA.18657.a.DG14.GE00191
HARVESTED ON: 11/3/2014
SEQUENCED ON: 9/30/2016
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSSNS SVLSLVNFTV DPQKAYLDFV NAGGAPLTNC VKMLTPKTGT GIAISVKPES TADQETYGGA SVCLYCRAHI EHPDVSGVCK YKGKFVQIPA QCVRDPVGFC LSNTPCNVCQ YWIGYGCNCD SLR
Validated NT Sequence
gacgcagaga atcacagttg cagccgtagc cgatccagta ctggcaaacg ttgcacggag tgttggacag gcagaaacct accgggtcac gaacgcactg tgcagggatc tgaacgaatt tacctttgta tttgcaaaca ccggaaacgt ccgggtgttc gatatgcgca cggcagtaca ggcagacaga cgcgccaccg taagtttcct ggtcagcagt agattccggt ttaacagaga tagctatgcc ggtaccagtc ttaggagtca gcatcttaac gcagttggtc agcggagcac cgcccgcgtt tacgaaatcc aggtatgcct tctgcgggtc gacagtaaag ttaaccagag acagtacaga agagttggag gatccaccga tttgttcacg gtgtgcttcg ataatgtcgt tatcttccat gtccagatct tccggagtct ggtctgcctg aatacggata ccgtcgtaca gaaagcgcag ggaatccatt tccttgccct ggcgtttagc gaatgcttcc atcaggcgac gcagcggggt ggtcttctta atcttgaaga agatttcgct gctaccgtcg ctaaccttca ggttgatgtg ggtctccggc ttcacttccg gttttacttc gccgctatgg tggtggtggt ggtgggacat
Expected Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSSNS SVLSLVNFTV DPQKAYLDFV NAGGAPLTNC VKMLTPKTGT GIAISVKPES TADQETYGGA SVCLYCRAHI EHPDVSGVCK YKGKFVQIPA QCVRDPVGFC LSNTPCNVCQ YWIGYGCNCD SLRQ
Full NT Sequence (Expression Vector + Insert)
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggatcc tccaactctt ctgtactgtc tctggttaac tttactgtcg acccgcagaa ggcatacctg gatttcgtaa acgcgggcgg tgctccgctg accaactgcg ttaagatgct gactcctaag actggtaccg gcatagctat ctctgttaaa ccggaatcta ctgctgacca ggaaacttac ggtggcgcgt ctgtctgcct gtactgccgt gcgcatatcg aacacccgga cgtttccggt gtttgcaaat acaaaggtaa attcgttcag atccctgcac agtgcgttcg tgacccggta ggtttctgcc tgtccaacac tccgtgcaac gtttgccagt actggatcgg ctacggctgc aactgtgatt ctctgcgtca gtgataagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaacttg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca aggagatggc gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctgctcatg tttgacagct tatc
Details for BecoA.18657.a.DG14.PD00375
PURIFICATION DATe: 6/17/2015
CONCENTRATION: 10.11mg/ml
OBSERVED MW: 25kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSSNS SVLSLVNFTV DPQKAYLDFV NAGGAPLTNC VKMLTPKTGT GIAISVKPES TADQETYGGA SVCLYCRAHI EHPDVSGVCK YKGKFVQIPA QCVRDPVGFC LSNTPCNVCQ YWIGYGCNCD SLR
Validated NT Sequence
gacgcagaga atcacagttg cagccgtagc cgatccagta ctggcaaacg ttgcacggag tgttggacag gcagaaacct accgggtcac gaacgcactg tgcagggatc tgaacgaatt tacctttgta tttgcaaaca ccggaaacgt ccgggtgttc gatatgcgca cggcagtaca ggcagacaga cgcgccaccg taagtttcct ggtcagcagt agattccggt ttaacagaga tagctatgcc ggtaccagtc ttaggagtca gcatcttaac gcagttggtc agcggagcac cgcccgcgtt tacgaaatcc aggtatgcct tctgcgggtc gacagtaaag ttaaccagag acagtacaga agagttggag gatccaccga tttgttcacg gtgtgcttcg ataatgtcgt tatcttccat gtccagatct tccggagtct ggtctgcctg aatacggata ccgtcgtaca gaaagcgcag ggaatccatt tccttgccct ggcgtttagc gaatgcttcc atcaggcgac gcagcggggt ggtcttctta atcttgaaga agatttcgct gctaccgtcg ctaaccttca ggttgatgtg ggtctccggc ttcacttccg gttttacttc gccgctatgg tggtggtggt ggtgggacat
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSSNS SVLSLVNFTV DPQKAYLDFV NAGGAPLTNC VKMLTPKTGT GIAISVKPES TADQETYGGA SVCLYCRAHI EHPDVSGVCK YKGKFVQIPA QCVRDPVGFC LSNTPCNVCQ YWIGYGCNCD SLRQ
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC TCCAACTCTT CTGTACTGTC TCTGGTTAAC TTTACTGTCG ACCCGCAGAA GGCATACCTG GATTTCGTAA ACGCGGGCGG TGCTCCGCTG ACCAACTGCG TTAAGATGCT GACTCCTAAG ACTGGTACCG GCATAGCTAT CTCTGTTAAA CCGGAATCTA CTGCTGACCA GGAAACTTAC GGTGGCGCGT CTGTCTGCCT GTACTGCCGT GCGCATATCG AACACCCGGA CGTTTCCGGT GTTTGCAAAT ACAAAGGTAA ATTCGTTCAG ATCCCTGCAC AGTGCGTTCG TGACCCGGTA GGTTTCTGCC TGTCCAACAC TCCGTGCAAC GTTTGCCAGT ACTGGATCGG CTACGGCTGC AACTGTGATT CTCTGCGTCA GTGATAAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTTG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATAGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCA AGGAGATGGC GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCTGCTCATG TTTGACAGCT TATC