BecoA.18656.a

ORF1ab nsp15

CENTER ID:	BecoA.18656.a
ORGANISM:	Betacoronavirus Human betacoronavirus 2c EMC/2012
ASSOCIATED DISEASE:	respiratory, GI infections
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BecoA.18656.a.DG13.PD00379	Full length( BecoA.18656.a )		27	344

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1235996.21.mat_peptide.15
UniProt:	K0BWD0
ViPR:	link

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

GLENIAFNVV KQGHFIGVEG ELPVAVVNDK IFTKSGVNDI CMFENKTTLP TNIAFELYAK RAVRSHPDFK LLHNLQADIC YKFVLWDYER SNIYGTATIG VCKYTDIDVN SALNICFDIR DNCSLEKFMS TPNAIFISDR KIKKYPCMVG PDYAYFNGAI IRDSDVVKQP VKFYLYKKVN NEFIDPTECI YTQSRSCSDF LPLSDMEKDF LSFDSDVFIK KYGLENYAFE HVVYGDFSHT TLGGLHLLIG LYKKQQEGHI IMEEMLKGSS TIHNYFITET NTAAFKAVCS VIDLKLDDFV MILKSQDLGV VSKVVKVPID LTMIEFMLWC KDGQVQTFYP RLQ

NT Sequence

GGTTTGGAAA ACATTGCTTT TAATGTTGTT AAACAAGGCC ATTTTATTGG TGTTGAGGGT GAACTACCTG TAGCTGTAGT CAATGATAAG ATCTTCACCA AGAGTGGCGT TAATGACATT TGTATGTTTG AGAATAAAAC CACTTTGCCT ACTAATATAG CTTTTGAACT CTATGCTAAG CGTGCTGTAC GCTCGCATCC CGATTTCAAA TTGCTACACA ATTTACAAGC AGACATTTGC TACAAGTTCG TCCTTTGGGA TTATGAACGT AGCAATATTT ATGGTACTGC TACTATTGGT GTATGTAAGT ACACTGATAT TGATGTTAAT TCAGCTTTGA ATATATGTTT TGACATACGC GATAATTGTT CATTGGAGAA GTTCATGTCT ACTCCCAATG CCATCTTTAT TTCTGATAGA AAAATCAAGA AATACCCTTG TATGGTAGGT CCTGATTATG CTTACTTCAA TGGTGCTATC ATCCGTGATA GTGATGTTGT TAAACAACCA GTGAAGTTCT ACTTGTATAA GAAAGTCAAT AATGAGTTTA TTGATCCTAC TGAGTGTATT TACACTCAGA GTCGCTCTTG TAGTGACTTC CTACCCCTTT CTGACATGGA GAAAGACTTT CTATCTTTTG ATAGTGATGT TTTCATTAAG AAGTATGGCT TGGAAAACTA TGCTTTTGAG CACGTAGTCT ATGGAGACTT CTCTCATACT ACGTTAGGCG GTCTTCACTT GCTTATTGGT TTATACAAGA AGCAACAGGA AGGTCATATT ATTATGGAAG AAATGCTAAA AGGTAGCTCA ACTATTCATA ACTATTTTAT TACTGAGACT AACACAGCGG CTTTTAAGGC GGTGTGTTCT GTTATAGATT TAAAGCTTGA CGACTTTGTT ATGATTTTAA AGAGTCAAGA CCTTGGCGTA GTATCCAAGG TTGTCAAGGT TCCTATTGAC TTAACAATGA TTGAGTTTAT GTTATGGTGT AAGGATGGAC AGGTTCAAAC CTTCTACCCT CGACTCCAG

Details for BecoA.18656.a.DG13.PD00379

PURIFICATION DATe:	6/17/2015
CONCENTRATION:	10mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	100µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSVND KIFTKSGVND ICMFENKTTL PTNIAFELYA KRAVRSHPDF KLLHNLQADI CYKFVLWDYE RSNIYGTATI GVCKYTDIDV NSALNICFDI RDNCSLEKFM STPNAIFISD RKIKKYPCMV GPDYAYFNGA IIRDSDVVKQ PVKFYLYKKV NNEFIDPTEC IYTQSRSCSD FLPLSDMEKD FLSFDSDVFI KKYGLENYAF EHVVYGDFSH TTLGGLHLLI GLYKKQQEGH IIMEEMLKGS STIHNYFITE TNTAAFKAVC SVIDLKLDDF VMILKSQDLG VVSKVVKVPI DLTMIEFMLW CKDGQVQTFY PRLQ

Full NT Sequence (Expression Vector + Insert)

ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC GTTAACGATA AGATTTTTAC GAAATCCGGT GTTAATGACA TCTGCATGTT CGAGAATAAG ACTACCCTGC CGACTAACAT AGCTTTCGAA CTGTACGCTA AGCGTGCTGT TCGCTCCCAC CCGGACTTCA AACTGCTGCA CAACCTGCAG GCTGACATCT GCTACAAATT TGTTCTGTGG GACTACGAGC GTTCTAACAT CTACGGCACC GCCACTATAG GCGTTTGCAA ATACACTGAT ATCGACGTAA ACTCCGCGCT GAACATCTGC TTCGATATCC GCGATAACTG TTCCTTAGAA AAATTCATGT CCACTCCGAA CGCTATCTTC ATCTCTGATC GTAAAATCAA AAAATACCCG TGCATGGTAG GCCCGGACTA CGCTTACTTT AACGGTGCAA TCATCCGTGA CTCCGACGTA GTTAAACAGC CGGTGAAATT CTACCTGTAC AAGAAGGTAA ACAACGAATT TATCGACCCG ACCGAGTGCA TCTATACTCA GTCCCGTTCC TGCTCTGACT TCCTGCCGCT GTCCGATATG GAGAAGGACT TCCTGAGCTT CGACTCTGAC GTCTTTATTA AGAAATATGG TCTGGAGAAC TACGCGTTTG AACACGTTGT GTACGGTGAC TTCTCTCACA CCACCTTAGG CGGCCTTCAC TTACTGATCG GTCTGTACAA GAAGCAGCAA GAAGGTCACA TCATCATGGA AGAAATGCTG AAGGGTAGCT CCACCATCCA CAACTACTTC ATCACGGAAA CCAACACCGC TGCATTCAAA GCTGTGTGCT CCGTTATCGA CCTGAAACTT GATGACTTCG TTATGATCCT GAAATCCCAG GACCTGGGTG TTGTTTCTAA AGTGGTTAAA GTTCCGATCG ATCTGACTAT GATAGAATTT ATGCTGTGGT GCAAAGACGG CCAGGTTCAG ACCTTCTACC CGCGTCTGCA ATGATAAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTTG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATAGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCA AGGAGATGGC GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCTGCTCATG TTTGACAGCT TATC