BecoA.18655.a

S protein (envelope protein S) (Spike glycoprotein)

CENTER ID: BecoA.18655.a
ORGANISM: Betacoronavirus Human betacoronavirus 2c EMC/2012
ASSOCIATED DISEASE: respiratory, GI infections
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BecoA.18655.a.HN11.GE00553 Full length( BecoA.18655.a ) 17 748
BecoA.18655.a.HN12.GE00554 Full length( BecoA.18655.a ) 28 748
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BecoA.18655.a.HN11.PD00485 Full length( BecoA.18655.a ) 17 748
Structures
6Q04
DEPOSITED: 8/1/2019
DETERMINATION: CryoEM
CLONE: BecoA.18655.a.MV5.GE43560
PROTEIN: BecoA.18655.a.MV5.PE00010
6Q05
DEPOSITED: 8/1/2019
DETERMINATION: CryoEM
CLONE: BecoA.18655.a.MV5.GE43560
PROTEIN: BecoA.18655.a.MV5.PE00010
6Q06
DEPOSITED: 8/1/2019
DETERMINATION: CryoEM
CLONE: BecoA.18655.a.MV5.GE43560
PROTEIN: BecoA.18655.a.MV5.PE00010
6Q07
DEPOSITED: 8/1/2019
DETERMINATION: CryoEM
CLONE: BecoA.18655.a.MV5.GE43560
PROTEIN: BecoA.18655.a.MV5.PE00010
7M5E
DEPOSITED: 3/23/2021
DETERMINATION: CryoEM
CLONE: BecoA.18655.a.MV5.GE43560
PROTEIN: BecoA.18655.a.MV5.PE00029
Publications by SSGCID
Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors
Tortorici MA, Walls AC, Bosch BJ, Veesler D, Li W, Park YJ, DiMaio F, Wang Z, Sauer MM
Nat. Struct. Mol. Biol. - 2019
volume 26, issue 12, pages 1151-1157
PMID: 31792450; PMCID: PMC7097669
PDB: 6Q04, 6Q05, 6Q06, 6Q07
Structural basis for broad coronavirus neutralization
Tortorici MA, Walls AC, McGuire AT, Bosch BJ, Veesler D, Xiong X, Park YJ, de van der Schueren W, King NP, Sauer MM, Bowen J, Acton O, Homad LJ, Wang C, Quispe J, Hoffstrom BG
Nat. Struct. Mol. Biol. - 2021
volume 82, issue 6, pages 478-486
PMID: 33981021
PDB: 7M51, 7M52, 7M53, 7M55, 7M5E
Automatic and accurate ligand structure determination guided by cryo-electron microscopy maps
Veesler D, DiMaio F, Zepeda S, Muenks A, Zhou G
Nat Commun - 2023
volume 14, issue 1, pages 1164
PMID: PMC9976687; PMCID: PMC9976687
PDB: 7M5E
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|1235996.21.CDS.3
UniProt: K0BRG7
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIHSVFLLMF LLTPTESYVD VGPDSVKSAC IEVDIQQTFF DKTWPRPIDV SKADGIIYPQ GRTYSNITIT YQGLFPYQGD HGDMYVYSAG HATGTTPQKL FVANYSQDVK QFANGFVVRI GAAANSTGTV IISPSTSATI RKIYPAFMLG SSVGNFSDGK MGRFFNHTLV LLPDGCGTLL RAFYCILEPR SGNHCPAGNS YTSFATYHTP ATDCSDGNYN RNASLNSFKE YFNLRNCTFM YTYNITEDEI LEWFGITQTA QGVHLFSSRY VDLYGGNMFQ FATLPVYDTI KYYSIIPHSI RSIQSDRKAW AAFYVYKLQP LTFLLDFSVD GYIRRAIDCG FNDLSQLHCS YESFDVESGV YSVSSFEAKP SGSVVEQAEG VECDFSPLLS GTPPQVYNFK RLVFTNCNYN LTKLLSLFSV NDFTCSQISP AAIASNCYSS LILDYFSYPL SMKSDLSVSS AGPISQFNYK QSFSNPTCLI LATVPHNLTT ITKPLKYSYI NKCSRLLSDD RTEVPQLVNA NQYSPCVSIV PSTVWEDGDY YRKQLSPLEG GGWLVASGST VAMTEQLQMG FGITVQYGTD TNSVCPKLEF ANDTKIASQL GNCVEYSLYG VSGRGVFQNC TAVGVRQQRF VYDAYQNLVG YYSDDGNYYC LRACVSVPVS VIYDKETKTH ATLFGSVACE HISSTMSQYS RSTRSMLKRR DSTYGPLQTP VGCVLGLVNS SLFVEDCKLP LGQSLCALPD TPSTLTPRSV RSVPGEMRLA SIAFNHPIQV DQLNSSYFKL SIPTNFSFGV TQEYIQTTIQ KVTVDCKQYV CNGFQKCEQL LREYGQFCSK INQALHGANL RQDDSVRNLF ASVKSSQSSP IIPGFGGDFN LTLLEPVSIS TGSRSARSAI EDLLFDKVTI ADPGYMQGYD DCMQQGPASA RDLICAQYVA GYKVLPPLMD VNMEAAYTSS LLGSIAGVGW TAGLSSFAAI PFAQSIFYRL NGVGITQQVL SENQKLIANK FNQALGAMQT GFTTTNEAFQ KVQDAVNNNA QALSKLASEL SNTFGAISAS IGDIIQRLDV LEQDAQIDRL INGRLTTLNA FVAQQLVRSE SAALSAQLAK DKVNECVKAQ SKRSGFCGQG THIVSFVVNA PNGLYFMHVG YYPSNHIEVV SAYGLCDAAN PTNCIAPVNG YFIKTNNTRI VDEWSYTGSS FYAPEPITSL NTKYVAPQVT YQNISTNLPP PLLGNSTGID FQDELDEFFK NVSTSIPNFG SLTQINTTLL DLTYEMLSLQ QVVKALNESY IDLKELGNYT YYNKWPWYIW LGFIAGLVAL ALCVFFILCC TGCGTNCMGK LKCNRCCDRY EEYDLEPHKV HVH
NT Sequence
ATGATACACT CAGTGTTTCT ACTGATGTTC TTGTTAACAC CTACAGAAAG TTACGTTGAT GTAGGGCCAG ATTCTGTTAA GTCTGCTTGT ATTGAGGTTG ATATACAACA GACTTTCTTT GATAAAACTT GGCCTAGGCC AATTGATGTT TCTAAGGCTG ACGGTATTAT ATACCCTCAA GGCCGTACAT ATTCTAACAT AACTATCACT TATCAAGGTC TTTTTCCCTA TCAGGGAGAC CATGGTGATA TGTATGTTTA CTCTGCAGGA CATGCTACAG GCACAACTCC ACAAAAGTTG TTTGTAGCTA ACTATTCTCA GGACGTCAAA CAGTTTGCTA ATGGGTTTGT CGTCCGTATA GGAGCAGCTG CCAATTCCAC TGGCACTGTT ATTATTAGCC CATCTACCAG CGCTACTATA CGAAAAATTT ACCCTGCTTT TATGCTGGGT TCTTCAGTTG GTAATTTCTC AGATGGTAAA ATGGGCCGCT TCTTCAATCA TACTCTAGTT CTTTTGCCCG ATGGATGTGG CACTTTACTT AGAGCTTTTT ATTGTATTCT AGAGCCTCGC TCTGGAAATC ATTGTCCTGC TGGCAATTCC TATACTTCTT TTGCCACTTA TCACACTCCT GCAACAGATT GTTCTGATGG CAATTACAAT CGTAATGCCA GTCTGAACTC TTTTAAGGAG TATTTTAATT TACGTAACTG CACCTTTATG TACACTTATA ACATTACCGA AGATGAGATT TTAGAGTGGT TTGGCATTAC ACAAACTGCT CAAGGTGTTC ACCTCTTCTC ATCTCGGTAT GTTGATTTGT ACGGCGGCAA TATGTTTCAA TTTGCCACCT TGCCTGTTTA TGATACTATT AAGTATTATT CTATCATTCC TCACAGTATT CGTTCTATCC AAAGTGATAG AAAAGCTTGG GCTGCCTTCT ACGTATATAA ACTTCAACCG TTAACTTTCC TGTTGGATTT TTCTGTTGAT GGTTATATAC GCAGAGCTAT AGACTGTGGT TTTAATGATT TGTCACAACT CCACTGCTCA TATGAATCCT TCGATGTTGA ATCTGGAGTT TATTCAGTTT CGTCTTTCGA AGCAAAACCT TCTGGCTCAG TTGTGGAACA GGCTGAAGGT GTTGAATGTG ATTTTTCACC TCTTCTGTCT GGCACACCTC CTCAGGTTTA TAATTTCAAG CGTTTGGTTT TTACCAATTG CAATTATAAT CTTACCAAAT TGCTTTCACT TTTTTCTGTG AATGATTTTA CTTGTAGTCA AATATCTCCA GCAGCAATTG CTAGCAACTG TTATTCTTCA CTGATTTTGG ATTACTTTTC ATACCCACTT AGTATGAAAT CCGATCTCAG TGTTAGTTCT GCTGGTCCAA TATCCCAGTT TAATTATAAA CAGTCCTTTT CTAATCCCAC ATGTTTGATT TTAGCGACTG TTCCTCATAA CCTTACTACT ATTACTAAGC CTCTTAAGTA CAGCTATATT AACAAGTGCT CTCGTCTTCT TTCTGATGAT CGTACTGAAG TACCTCAGTT AGTGAACGCT AATCAATACT CACCCTGTGT ATCCATTGTC CCATCCACTG TGTGGGAAGA CGGTGATTAT TATAGGAAAC AACTATCTCC ACTTGAAGGT GGTGGCTGGC TTGTTGCTAG TGGCTCAACT GTTGCCATGA CTGAGCAATT ACAGATGGGC TTTGGTATTA CAGTTCAATA TGGTACAGAC ACCAATAGTG TTTGCCCCAA GCTTGAATTT GCTAATGACA CAAAAATTGC CTCTCAATTA GGCAATTGCG TGGAATATTC CCTCTATGGT GTTTCGGGCC GTGGTGTTTT TCAGAATTGC ACAGCTGTAG GTGTTCGACA GCAGCGCTTT GTTTATGATG CGTACCAGAA TTTAGTTGGC TATTATTCTG ATGATGGCAA CTACTACTGT TTGCGTGCTT GTGTTAGTGT TCCTGTTTCT GTCATCTATG ATAAAGAAAC TAAAACCCAC GCTACTCTAT TTGGTAGTGT TGCATGTGAA CACATTTCTT CTACCATGTC TCAATACTCC CGTTCTACGC GATCAATGCT TAAACGGCGA GATTCTACAT ATGGCCCCCT TCAGACACCT GTTGGTTGTG TCCTAGGACT TGTTAATTCC TCTTTGTTCG TAGAGGACTG CAAGTTGCCT CTTGGTCAAT CTCTCTGTGC TCTTCCTGAC ACACCTAGTA CTCTCACACC TCGCAGTGTG CGCTCTGTTC CAGGTGAAAT GCGCTTGGCA TCCATTGCTT TTAATCATCC TATTCAGGTT GATCAACTTA ATAGTAGTTA TTTTAAATTA AGTATACCCA CTAATTTTTC CTTTGGTGTG ACTCAGGAGT ACATTCAGAC AACCATTCAG AAAGTTACTG TTGATTGTAA ACAGTACGTT TGCAATGGTT TCCAGAAGTG TGAGCAATTA CTGCGCGAGT ATGGCCAGTT TTGTTCCAAA ATAAACCAGG CTCTCCATGG TGCCAATTTA CGCCAGGATG ATTCTGTACG TAATTTGTTT GCGAGCGTGA AAAGCTCTCA ATCATCTCCT ATCATACCAG GTTTTGGAGG TGACTTTAAT TTGACACTTC TAGAACCTGT TTCTATATCT ACTGGCAGTC GTAGTGCACG TAGTGCTATT GAGGATTTGC TATTTGACAA AGTCACTATA GCTGATCCTG GTTATATGCA AGGTTACGAT GATTGCATGC AGCAAGGTCC AGCATCAGCT CGTGATCTTA TTTGTGCTCA ATATGTGGCT GGTTACAAAG TATTACCTCC TCTTATGGAT GTTAATATGG AAGCCGCGTA TACTTCATCT TTGCTTGGCA GCATAGCAGG TGTTGGCTGG ACTGCTGGCT TATCCTCCTT TGCTGCTATT CCATTTGCAC AGAGTATCTT TTATAGGTTA AACGGTGTTG GCATTACTCA ACAGGTTCTT TCAGAGAACC AAAAGCTTAT TGCCAATAAG TTTAATCAGG CTCTGGGAGC TATGCAAACA GGCTTCACTA CAACTAATGA AGCTTTTCAG AAGGTTCAGG ATGCTGTGAA CAACAATGCA CAGGCTCTAT CCAAATTAGC TAGCGAGCTA TCTAATACTT TTGGTGCTAT TTCCGCCTCT ATTGGAGACA TCATACAACG TCTTGATGTT CTCGAACAGG ACGCCCAAAT AGACAGACTT ATTAATGGCC GTTTGACAAC ACTAAATGCT TTTGTTGCAC AGCAGCTTGT TCGTTCCGAA TCAGCTGCTC TTTCCGCTCA ATTGGCTAAA GATAAAGTCA ATGAGTGTGT CAAGGCACAA TCCAAGCGTT CTGGATTTTG CGGTCAAGGC ACACATATAG TGTCCTTTGT TGTAAATGCC CCTAATGGCC TTTACTTCAT GCATGTTGGT TATTACCCTA GCAACCACAT TGAGGTTGTT TCTGCTTATG GTCTTTGCGA TGCAGCTAAC CCTACTAATT GTATAGCCCC TGTTAATGGC TACTTTATTA AAACTAATAA CACTAGGATT GTTGATGAGT GGTCATATAC TGGCTCGTCC TTCTATGCAC CTGAGCCCAT TACCTCCCTT AATACTAAGT ATGTTGCACC ACAGGTGACA TACCAAAACA TTTCTACTAA CCTCCCTCCT CCTCTTCTCG GCAATTCCAC CGGGATTGAC TTCCAAGATG AGTTGGATGA GTTTTTCAAA AATGTTAGCA CCAGTATACC TAATTTTGGT TCCCTAACAC AGATTAATAC TACATTACTC GATCTTACCT ACGAGATGTT GTCTCTTCAA CAAGTTGTTA AAGCCCTTAA TGAGTCTTAC ATAGACCTTA AAGAGCTTGG CAATTATACT TATTACAACA AATGGCCGTG GTACATTTGG CTTGGTTTCA TTGCTGGGCT TGTTGCCTTA GCTCTATGCG TCTTCTTCAT ACTGTGCTGC ACTGGTTGTG GCACAAACTG TATGGGAAAA CTTAAGTGTA ATCGTTGTTG TGATAGATAC GAGGAATACG ACCTCGAGCC GCATAAGGTT CATGTTCAC
Details for BecoA.18655.a.HN11.GE00553
HARVESTED ON: 2/16/2016
SEQUENCED ON: 5/4/2017
EXPECTED MW: 86kDa
OBSERVED MW: 86kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Data Unavailable
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MLLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAGS SYVDVGPDSV KSACIEVDIQ QTFFDKTWPR PIDVSKADGI IYPQGRTYSN ITITYQGLFP YQGDHGDMYV YSAGHATGTT PQKLFVANYS QDVKQFANGF VVRIGAAANS TGTVIISPST SATIRKIYPA FMLGSSVGNF SDGKMGRFFN HTLVLLPDGC GTLLRAFYCI LEPRSGNHCP AGNSYTSFAT YHTPATDCSD GNYNRNASLN SFKEYFNLRN CTFMYTYNIT EDEILEWFGI TQTAQGVHLF SSRYVDLYGG NMFQFATLPV YDTIKYYSII PHSIRSIQSD RKAWAAFYVY KLQPLTFLLD FSVDGYIRRA IDCGFNDLSQ LHCSYESFDV ESGVYSVSSF EAKPSGSVVE QAEGVECDFS PLLSGTPPQV YNFKRLVFTN CNYNLTKLLS LFSVNDFTCS QISPAAIASN CYSSLILDYF SYPLSMKSDL SVSSAGPISQ FNYKQSFSNP TCLILATVPH NLTTITKPLK YSYINKCSRL LSDDRTEVPQ LVNANQYSPC VSIVPSTVWE DGDYYRKQLS PLEGGGWLVA SGSTVAMTEQ LQMGFGITVQ YGTDTNSVCP KLEFANDTKI ASQLGNCVEY SLYGVSGRGV FQNCTAVGVR QQRFVYDAYQ NLVGYYSDDG NYYCLRACVS VPVSVIYDKE TKTHATLFGS VACEHISSTM SQYSRSTRSM LKRRDSTYGP LQTPVGCVLG LVNSSLFVED CKLPLGQSLC ALPDTPSTLT PLEHHHHHH
Full NT Sequence (Expression Vector + Insert)
ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg ctactagtaa atcagtcaca ccaaggcttc aataaggaac acacaagcaa gatggtaagc gctattgttt tatatgtgct tttggcggcg gcggcgcatt ctgcctttgc gggatcctct tacgtagacg taggcccgga ctccgtgaaa tccgcctgca ttgaagtaga tatccagcaa accttctttg ataagacctg gccacgtccg atcgacgttt ctaaggccga tggtatcatc tacccgcagg gtcgcaccta ctccaatatc accattacct accagggtct gttcccttac cagggtgacc acggcgacat gtacgtttac tctgcaggcc atgctaccgg taccactccg cagaagctgt tcgtagctaa ctactctcaa gacgtgaagc agttcgctaa cggctttgtt gtccgcatcg gcgctgccgc taacagcacc ggcactgtga tcatctctcc gtctacctct gctaccatcc gtaaaatcta cccggcattc atgctgggtt cctctgtagg caacttcagc gacggcaaga tgggccgttt cttcaaccac accctggttc ttctgcctga cggttgtggt actctcctgc gtgcattcta ctgcatcctc gaaccgcgtt ccggcaacca ctgcccagct ggtaactcct acaccagctt tgcaacctac cacactcctg ccaccgactg ctctgacggc aactacaatc gtaacgcatc ccttaactcc ttcaaagaat actttaacct ccgtaactgc accttcatgt atacctacaa cattaccgag gatgaaatcc tggagtggtt tggtatcact cagactgccc agggtgtgca cctgtttagc tctcgttacg ttgacctcta cggtggcaac atgttccagt tcgcaactct ccctgtgtac gataccatta agtactactc cattattccg cactctatcc gtagcatcca gtccgatcgc aaagcatggg ctgcattcta cgtgtataaa ctgcagccgc tgaccttcct cctcgatttc agcgtggatg gttacatccg tcgcgccatc gactgcggtt tcaacgacct gagccagctg cactgtagct acgagtcctt cgacgttgaa tccggtgtct actctgttag ctcctttgaa gctaagccat ctggctccgt agttgaacag gcagagggcg tggagtgtga cttctctcct ctcctgagcg gtactccgcc gcaggtttac aatttcaaac gtctggtatt caccaactgc aactacaacc tgactaagct gctgtccctc ttctctgtta atgacttcac ttgctcccag atcagccctg ccgccatcgc aagcaactgc tattcctccc tgatcctgga ttatttctct tacccactca gcatgaagtc tgacctgtct gtgtcctctg ctggcccaat ctcccagttc aactacaagc agtccttctc caatccgacc tgcctgatcc tggcaactgt accgcataac ctcactacca tcactaaacc gctgaaatac tcctacatca acaaatgctc ccgcctgctg tctgacgatc gtactgaagt tcctcagctg gtgaacgcta accagtattc tccttgcgtc tctatcgtac cgtccaccgt ttgggaagat ggtgactact accgtaaaca gctgagccca ctcgaaggtg gcggttggct tgttgcttcc ggctctactg ttgcaatgac tgaacagctg cagatgggct tcggcatcac cgtgcagtat ggcaccgata ccaactccgt ttgcccaaag ctggaatttg caaacgacac caagatcgct agccagctgg gtaactgcgt tgagtactct ctgtacggtg tatctggtcg cggtgtcttt cagaattgta ccgctgtagg cgttcgtcag cagcgtttcg tttacgacgc ctaccagaac ctcgtgggct actatagcga cgacggtaat tactactgcc tgcgtgcatg cgtatccgtg ccagtgtccg tgatctacga taaggagact aagactcacg caaccctgtt tggttccgta gcttgcgaac acattagctc taccatgtcc caatacagcc gttctactcg ttccatgctg aaacgtcgtg attccactta cggccctctg cagactcctg taggctgcgt gctgggtctg gtgaactctt ctctgtttgt tgaagactgt aaactcccgc tgggccagtc tctgtgcgct ctgccggaca ctccgtccac cctgactcct ctcgagcacc accaccacca ccactaacct aggtagctga gcgcatgcaa gctgatccgg gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaatagagc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt attgtaaaga gattgtctca agctcggatc gatcccgcac gccgataaca agccttttca tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgaggttt aaacgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgcca
Details for BecoA.18655.a.HN12.GE00554
HARVESTED ON: 2/16/2016
SEQUENCED ON: 5/4/2017
EXPECTED MW: 85kDa
OBSERVED MW: 85kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Data Unavailable
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MLLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAGS SACIEVDIQQ TFFDKTWPRP IDVSKADGII YPQGRTYSNI TITYQGLFPY QGDHGDMYVY SAGHATGTTP QKLFVANYSQ DVKQFANGFV VRIGAAANST GTVIISPSTS ATIRKIYPAF MLGSSVGNFS DGKMGRFFNH TLVLLPDGCG TLLRAFYCIL EPRSGNHCPA GNSYTSFATY HTPATDCSDG NYNRNASLNS FKEYFNLRNC TFMYTYNITE DEILEWFGIT QTAQGVHLFS SRYVDLYGGN MFQFATLPVY DTIKYYSIIP HSIRSIQSDR KAWAAFYVYK LQPLTFLLDF SVDGYIRRAI DCGFNDLSQL HCSYESFDVE SGVYSVSSFE AKPSGSVVEQ AEGVECDFSP LLSGTPPQVY NFKRLVFTNC NYNLTKLLSL FSVNDFTCSQ ISPAAIASNC YSSLILDYFS YPLSMKSDLS VSSAGPISQF NYKQSFSNPT CLILATVPHN LTTITKPLKY SYINKCSRLL SDDRTEVPQL VNANQYSPCV SIVPSTVWED GDYYRKQLSP LEGGGWLVAS GSTVAMTEQL QMGFGITVQY GTDTNSVCPK LEFANDTKIA SQLGNCVEYS LYGVSGRGVF QNCTAVGVRQ QRFVYDAYQN LVGYYSDDGN YYCLRACVSV PVSVIYDKET KTHATLFGSV ACEHISSTMS QYSRSTRSML KRRDSTYGPL QTPVGCVLGL VNSSLFVEDC KLPLGQSLCA LPDTPSTLTP LEHHHHHH
Full NT Sequence (Expression Vector + Insert)
ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg ctactagtaa atcagtcaca ccaaggcttc aataaggaac acacaagcaa gatggtaagc gctattgttt tatatgtgct tttggcggcg gcggcgcatt ctgcctttgc gggatcctcc gcctgcattg aagtagatat ccagcaaacc ttctttgata agacctggcc acgtccgatc gacgtttcta aggccgatgg tatcatctac ccgcagggtc gcacctactc caatatcacc attacctacc agggtctgtt cccttaccag ggtgaccacg gcgacatgta cgtttactct gcaggccatg ctaccggtac cactccgcag aagctgttcg tagctaacta ctctcaagac gtgaagcagt tcgctaacgg ctttgttgtc cgcatcggcg ctgccgctaa cagcaccggc actgtgatca tctctccgtc tacctctgct accatccgta aaatctaccc ggcattcatg ctgggttcct ctgtaggcaa cttcagcgac ggcaagatgg gccgtttctt caaccacacc ctggttcttc tgcctgacgg ttgtggtact ctcctgcgtg cattctactg catcctcgaa ccgcgttccg gcaaccactg cccagctggt aactcctaca ccagctttgc aacctaccac actcctgcca ccgactgctc tgacggcaac tacaatcgta acgcatccct taactccttc aaagaatact ttaacctccg taactgcacc ttcatgtata cctacaacat taccgaggat gaaatcctgg agtggtttgg tatcactcag actgcccagg gtgtgcacct gtttagctct cgttacgttg acctctacgg tggcaacatg ttccagttcg caactctccc tgtgtacgat accattaagt actactccat tattccgcac tctatccgta gcatccagtc cgatcgcaaa gcatgggctg cattctacgt gtataaactg cagccgctga ccttcctcct cgatttcagc gtggatggtt acatccgtcg cgccatcgac tgcggtttca acgacctgag ccagctgcac tgtagctacg agtccttcga cgttgaatcc ggtgtctact ctgttagctc ctttgaagct aagccatctg gctccgtagt tgaacaggca gagggcgtgg agtgtgactt ctctcctctc ctgagcggta ctccgccgca ggtttacaat ttcaaacgtc tggtattcac caactgcaac tacaacctga ctaagctgct gtccctcttc tctgttaatg acttcacttg ctcccagatc agccctgccg ccatcgcaag caactgctat tcctccctga tcctggatta tttctcttac ccactcagca tgaagtctga cctgtctgtg tcctctgctg gcccaatctc ccagttcaac tacaagcagt ccttctccaa tccgacctgc ctgatcctgg caactgtacc gcataacctc actaccatca ctaaaccgct gaaatactcc tacatcaaca aatgctcccg cctgctgtct gacgatcgta ctgaagttcc tcagctggtg aacgctaacc agtattctcc ttgcgtctct atcgtaccgt ccaccgtttg ggaagatggt gactactacc gtaaacagct gagcccactc gaaggtggcg gttggcttgt tgcttccggc tctactgttg caatgactga acagctgcag atgggcttcg gcatcaccgt gcagtatggc accgatacca actccgtttg cccaaagctg gaatttgcaa acgacaccaa gatcgctagc cagctgggta actgcgttga gtactctctg tacggtgtat ctggtcgcgg tgtctttcag aattgtaccg ctgtaggcgt tcgtcagcag cgtttcgttt acgacgccta ccagaacctc gtgggctact atagcgacga cggtaattac tactgcctgc gtgcatgcgt atccgtgcca gtgtccgtga tctacgataa ggagactaag actcacgcaa ccctgtttgg ttccgtagct tgcgaacaca ttagctctac catgtcccaa tacagccgtt ctactcgttc catgctgaaa cgtcgtgatt ccacttacgg ccctctgcag actcctgtag gctgcgtgct gggtctggtg aactcttctc tgtttgttga agactgtaaa ctcccgctgg gccagtctct gtgcgctctg ccggacactc cgtccaccct gactcctctc gagcaccacc accaccacca ctaacctagg tagctgagcg catgcaagct gatccgggtt attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa atagagcttg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcggatcgat cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc gaggtttaaa cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gcca
Details for BecoA.18655.a.HN11.PD00485
PURIFICATION DATe: 3/5/2017
CONCENTRATION: 19.48mg/ml
OBSERVED MW: 86kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM Tris pH 8.0, 200 mM NaCl, 1% glycerol, 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 50µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MLLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAGS SYVDVGPDSV KSACIEVDIQ QTFFDKTWPR PIDVSKADGI IYPQGRTYSN ITITYQGLFP YQGDHGDMYV YSAGHATGTT PQKLFVANYS QDVKQFANGF VVRIGAAANS TGTVIISPST SATIRKIYPA FMLGSSVGNF SDGKMGRFFN HTLVLLPDGC GTLLRAFYCI LEPRSGNHCP AGNSYTSFAT YHTPATDCSD GNYNRNASLN SFKEYFNLRN CTFMYTYNIT EDEILEWFGI TQTAQGVHLF SSRYVDLYGG NMFQFATLPV YDTIKYYSII PHSIRSIQSD RKAWAAFYVY KLQPLTFLLD FSVDGYIRRA IDCGFNDLSQ LHCSYESFDV ESGVYSVSSF EAKPSGSVVE QAEGVECDFS PLLSGTPPQV YNFKRLVFTN CNYNLTKLLS LFSVNDFTCS QISPAAIASN CYSSLILDYF SYPLSMKSDL SVSSAGPISQ FNYKQSFSNP TCLILATVPH NLTTITKPLK YSYINKCSRL LSDDRTEVPQ LVNANQYSPC VSIVPSTVWE DGDYYRKQLS PLEGGGWLVA SGSTVAMTEQ LQMGFGITVQ YGTDTNSVCP KLEFANDTKI ASQLGNCVEY SLYGVSGRGV FQNCTAVGVR QQRFVYDAYQ NLVGYYSDDG NYYCLRACVS VPVSVIYDKE TKTHATLFGS VACEHISSTM SQYSRSTRSM LKRRDSTYGP LQTPVGCVLG LVNSSLFVED CKLPLGQSLC ALPDTPSTLT PLEHHHHHH
Full NT Sequence (Expression Vector + Insert)
GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA TCAATATATA GTTGCTGATC AGATCCTCAT TGTTTGCCTC CCTGCTGCGG TTTTTCACCG AAGTTCATGC CAGTCCAGCG TTTTTGCAGC AGAAAAGCCG CCGACTTCGG TTTGCGGTCG CGAGTGAAGA TCCCTTTCTT GTTACCGCCA ACGCGCAATA TGCCTTGCGA GGTCGCAAAA TCGGCGAAAT TCCATACCTG TTCACCGACG ACGGCGCTGA CGCGATCAAA GACGCGGTGA TACATATCCA GCCATGCACA CTGATACTCT TCACTCCACA TGTCGGTGTA CATTGAGTGC AGCCCGGCTA ACGTATCCAC GCCGTATTCG GTGATGATAA TCGGCTGATG CAGTTTCTCC TGCCAGGCCA GAAGTTCTTT TTCCAGTACC TTCTCTGCCG TTTCCAAATC GCCGCTTTGG ACATACCATC CGTAATAACG GTTCAGGCAC AGCACATCAA AGAGATCGCT GATGGTATCG GTGTGAGCGT CGCAGAACAT TACATTGACG CAGGTGATCG GACGCGTCGG GTCGAGTTTA CGCGTTGCTT CCGCCAGTGG CGCGAAATAT TCCCGTGCAC CTTGCGGACG GGTATCCGGT TCGTTGGCAA TACTCCACAT CACCACGCTT GGGTGGTTTT TGTCACGCGC TATCAGCTCT TTAATCGCCT GTAAGTGCGC TTGCTGAGTT TCCCCGTTGA CTGCCTCTTC GCTGTACAGT TCTTTCGGCT TGTTGCCCGC TTCGAAACCA ATGCCTAAAG AGAGCGAAAA GCCGACAGCA GCAGTTTCAT CAATCACCAC GATGCCATGT TCATCTGCCC AGTCGAGCAT CTCTTCAGCG TAAGGGTAAT GCGAGGTACG GTAGGAGTTG GCCCCAATCC AGTCCATTAA TGCGTGGTCG TGCACCATCA GCACGTTATC GAATCCTTTG CCACGTAAGT CCGCATCTTC ATGACGACCA AAGCCAGTAA AGTAGAACGG TTTGTGGTTA ATCAGGAACT GTTGGCCCTT CACTGCCACT GACCGGATGC CGACGCGAAG CGGGTAGATA TCACACTCTG TCTGGCTTTT GGCTGTGACG CACAGTTCAT AGAGATAACC TTCACCCGGT TGCCAGAGGT GCGGATTCAC CACTTGCAAA GTCCCGCTAG TGCCTTGTCC AGTTGCAACC ACCTGTTGAT CCGCATCACG CAGTTCAACG CTGACATCAC CATTGGCCAC CACCTGCCAG TCAACAGACG CGTGGTTACA GTCTTGCGCG ACATGCGTCA CCACGGTGAT ATCGTCCACC CAGGTGTTCG GCGTGGTGTA GAGCATTACG CTGCGATGGA TTCCGGCATA GTTAAAGAAA TCATGGAAGT AAGACTGCTT TTTCTTGCCG TTTTCGTCGG TAATCACCAT TCCCGGCGGG ATAGTCTGCC AGTTCAGTTC GTTGTTCACA CAAACGGTGA TACGTACACT TTTCCCGGCA ATAACATACG GCGTGACATC GGCTTCAAAT GGCGTATAGC CGCCCTGATG CTCCATCACT TCCTGATTAT TGACCCACAC TTTGCCGTAA TGAGTGACCG CATCGAAACG CAGCACGATA CGCTGGCCTG CCCAACCTTT CGGTATAAAG ACTTCGCGCT GATACCAGAC GTTGCCCGCA TAATTACGAA TATCTGCATC GGCGAACTGA TCGTTAAAAC TGCCTGGCAC AGCAATTGCC CGGCTTTCTT GTAACGCGCT TTCCCACCAA CGCTGATCAA TTCCACAGTT TTCGCGATCC AGACTGAATG CCCACAGGCC GTCGAGTTTT TTGATTTCAC GGGTTGGGGT TTCTACAGGA CGGACCATAT TTATAGGTGT AATTTATGTA GCTGTAATTT TTACCTTATT AATATTTTTT ACGCTTTGCA TTCGACGACT GAACTCCCAA ATATATGTTT AACTCGTCTT GGTCGTTTGA ATTTTTGTTG CTGTGTTTCC TAATATTTTC CATCACCTTA AATATGTTAT TGTAATCCTC AATGTTGAAC TTGCAATTGG ACACGGCATA GTTTTCCATA GTCGTGTAAA ACATGGTATT GGCTGCATTG TAATACATCC GACTGAGCGG GTACGGATCT ATGTGTTTGA GCAGCCTGTT CAAAAACTCT GCATCGTCGC AAAACGGAAT TTCGTCGACA GATCTGATCA TGGAGATAAT TAAAATGATA ACCATCTCGC AAATAAATAA GTATTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT AGGATCTATG CTACTAGTAA ATCAGTCACA CCAAGGCTTC AATAAGGAAC ACACAAGCAA GATGGTAAGC GCTATTGTTT TATATGTGCT TTTGGCGGCG GCGGCGCATT CTGCCTTTGC GGGATCCTCT TACGTAGACG TAGGCCCGGA CTCCGTGAAA TCCGCCTGCA TTGAAGTAGA TATCCAGCAA ACCTTCTTTG ATAAGACCTG GCCACGTCCG ATCGACGTTT CTAAGGCCGA TGGTATCATC TACCCGCAGG GTCGCACCTA CTCCAATATC ACCATTACCT ACCAGGGTCT GTTCCCTTAC CAGGGTGACC ACGGCGACAT GTACGTTTAC TCTGCAGGCC ATGCTACCGG TACCACTCCG CAGAAGCTGT TCGTAGCTAA CTACTCTCAA GACGTGAAGC AGTTCGCTAA CGGCTTTGTT GTCCGCATCG GCGCTGCCGC TAACAGCACC GGCACTGTGA TCATCTCTCC GTCTACCTCT GCTACCATCC GTAAAATCTA CCCGGCATTC ATGCTGGGTT CCTCTGTAGG CAACTTCAGC GACGGCAAGA TGGGCCGTTT CTTCAACCAC ACCCTGGTTC TTCTGCCTGA CGGTTGTGGT ACTCTCCTGC GTGCATTCTA CTGCATCCTC GAACCGCGTT CCGGCAACCA CTGCCCAGCT GGTAACTCCT ACACCAGCTT TGCAACCTAC CACACTCCTG CCACCGACTG CTCTGACGGC AACTACAATC GTAACGCATC CCTTAACTCC TTCAAAGAAT ACTTTAACCT CCGTAACTGC ACCTTCATGT ATACCTACAA CATTACCGAG GATGAAATCC TGGAGTGGTT TGGTATCACT CAGACTGCCC AGGGTGTGCA CCTGTTTAGC TCTCGTTACG TTGACCTCTA CGGTGGCAAC ATGTTCCAGT TCGCAACTCT CCCTGTGTAC GATACCATTA AGTACTACTC CATTATTCCG CACTCTATCC GTAGCATCCA GTCCGATCGC AAAGCATGGG CTGCATTCTA CGTGTATAAA CTGCAGCCGC TGACCTTCCT CCTCGATTTC AGCGTGGATG GTTACATCCG TCGCGCCATC GACTGCGGTT TCAACGACCT GAGCCAGCTG CACTGTAGCT ACGAGTCCTT CGACGTTGAA TCCGGTGTCT ACTCTGTTAG CTCCTTTGAA GCTAAGCCAT CTGGCTCCGT AGTTGAACAG GCAGAGGGCG TGGAGTGTGA CTTCTCTCCT CTCCTGAGCG GTACTCCGCC GCAGGTTTAC AATTTCAAAC GTCTGGTATT CACCAACTGC AACTACAACC TGACTAAGCT GCTGTCCCTC TTCTCTGTTA ATGACTTCAC TTGCTCCCAG ATCAGCCCTG CCGCCATCGC AAGCAACTGC TATTCCTCCC TGATCCTGGA TTATTTCTCT TACCCACTCA GCATGAAGTC TGACCTGTCT GTGTCCTCTG CTGGCCCAAT CTCCCAGTTC AACTACAAGC AGTCCTTCTC CAATCCGACC TGCCTGATCC TGGCAACTGT ACCGCATAAC CTCACTACCA TCACTAAACC GCTGAAATAC TCCTACATCA ACAAATGCTC CCGCCTGCTG TCTGACGATC GTACTGAAGT TCCTCAGCTG GTGAACGCTA ACCAGTATTC TCCTTGCGTC TCTATCGTAC CGTCCACCGT TTGGGAAGAT GGTGACTACT ACCGTAAACA GCTGAGCCCA CTCGAAGGTG GCGGTTGGCT TGTTGCTTCC GGCTCTACTG TTGCAATGAC TGAACAGCTG CAGATGGGCT TCGGCATCAC CGTGCAGTAT GGCACCGATA CCAACTCCGT TTGCCCAAAG CTGGAATTTG CAAACGACAC CAAGATCGCT AGCCAGCTGG GTAACTGCGT TGAGTACTCT CTGTACGGTG TATCTGGTCG CGGTGTCTTT CAGAATTGTA CCGCTGTAGG CGTTCGTCAG CAGCGTTTCG TTTACGACGC CTACCAGAAC CTCGTGGGCT ACTATAGCGA CGACGGTAAT TACTACTGCC TGCGTGCATG CGTATCCGTG CCAGTGTCCG TGATCTACGA TAAGGAGACT AAGACTCACG CAACCCTGTT TGGTTCCGTA GCTTGCGAAC ACATTAGCTC TACCATGTCC CAATACAGCC GTTCTACTCG TTCCATGCTG AAACGTCGTG ATTCCACTTA CGGCCCTCTG CAGACTCCTG TAGGCTGCGT GCTGGGTCTG GTGAACTCTT CTCTGTTTGT TGAAGACTGT AAACTCCCGC TGGGCCAGTC TCTGTGCGCT CTGCCGGACA CTCCGTCCAC CCTGACTCCT CTCGAGCACC ACCACCACCA CCACTAACCT AGGTAGCTGA GCGCATGCAA GCTGATCCGG GTTATTAGTA CATTTATTAA GCGCTAGATT CTGTGCGTTG TTGATTTACA GACAATTGTT GTACGTATTT TAATAATTCA TTAAATTTAT AATCTTTAGG GTGGTATGTT AGAGCGAAAA TCAAATGATT TTCAGCGTCT TTATATCTGA ATTTAAATAT TAAATCCTCA ATAGATTTGT AAAATAGGTT TCGATTAGTT TCAAACAAGG GTTGTTTTTC CGAACCGATG GCTGGACTAT CTAATGGATT TTCGCTCAAC GCCACAAAAC TTGCCAAATC TTGTAGCAGC AATCTAGCTT TGTCGATATT CGTTTGTGTT TTGTTTTGTA ATAAAGGTTC GACGTCGTTC AAAATATTAT GCGCTTTTGT ATTTCTTTCA TCACTGTCGT TAGTGTACAA TTGACTCGAC GTAAACACGT TAAATAGAGC TTGGACATAT TTAACATCGG GCGTGTTAGC TTTATTAGGC CGATTATCGT CGTCGTCCCA ACCCTCGTCG TTAGAAGTTG CTTCCGAAGA CGATTTTGCC ATAGCCACAC GACGCCTATT AATTGTGTCG GCTAACACGT CCGCGATCAA ATTTGTAGTT GAGCTTTTTG GAATTATTTC TGATTGCGGG CGTTTTTGGG CGGGTTTCAA TCTAACTGTG CCCGATTTTA ATTCAGACAA CACGTTAGAA AGCGATGGTG CAGGCGGTGG TAACATTTCA GACGGCAAAT CTACTAATGG CGGCGGTGGT GGAGCTGATG ATAAATCTAC CATCGGTGGA GGCGCAGGCG GGGCTGGCGG CGGAGGCGGA GGCGGAGGTG GTGGCGGTGA TGCAGACGGC GGTTTAGGCT CAAATGTCTC TTTAGGCAAC ACAGTCGGCA CCTCAACTAT TGTACTGGTT TCGGGCGCCG TTTTTGGTTT GACCGGTCTG AGACGAGTGC GATTTTTTTC GTTTCTAATA GCTTCCAACA ATTGTTGTCT GTCGTCTAAA GGTGCAGCGG GTTGAGGTTC CGTCGGCATT GGTGGAGCGG GCGGCAATTC AGACATCGAT GGTGGTGGTG GTGGTGGAGG CGCTGGAATG TTAGGCACGG GAGAAGGTGG TGGCGGCGGT GCCGCCGGTA TAATTTGTTC TGGTTTAGTT TGTTCGCGCA CGATTGTGGG CACCGGCGCA GGCGCCGCTG GCTGCACAAC GGAAGGTCGT CTGCTTCGAG GCAGCGCTTG GGGTGGTGGC AATTCAATAT TATAATTGGA ATACAAATCG TAAAAATCTG CTATAAGCAT TGTAATTTCG CTATCGTTTA CCGTGCCGAT ATTTAACAAC CGCTCAATGT AAGCAATTGT ATTGTAAAGA GATTGTCTCA AGCTCGGATC GATCCCGCAC GCCGATAACA AGCCTTTTCA TTTTTACTAC AGCATTGTAG TGGCGAGACA CTTCGCTGTC GTCGAGGTTT AAACGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCA