BecoA.18654.a

ORF1ab nsp16

CENTER ID: BecoA.18654.a
ORGANISM: Betacoronavirus Human betacoronavirus 2c EMC/2012
ASSOCIATED DISEASE: respiratory, GI infections
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BecoA.18654.a.DG14.PD00324 Full length( BecoA.18654.a ) 3 295 unavailable
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|1235996.21.mat_peptide.16
UniProt: K0BWD0
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
ASADWKPGHA MPSLFKVQNV NLERCELANY KQSIPMPRGV HMNIAKYMQL CQYLNTCTLA VPANMRVIHF GAGSDKGIAP GTSVLRQWLP TDAIIIDNDL NEFVSDADIT LFGDCVTVRV GQQVDLVISD MYDPTTKNVT GSNESKALFF TYLCNLINNN LALGGSVAIK ITEHSWSVEL YELMGKFAWW TVFCTNANAS SSEGFLLGIN YLGTIKENID GGAMHANYIF WRNSTPMNLS TYSLFDLSKF QLKLKGTPVL QLKESQINEL VISLLSQGKL LIRDNDTLSV STDVLVNTYR KLR
NT Sequence
GCTTCTGCAG ATTGGAAACC TGGTCATGCA ATGCCATCCC TCTTTAAAGT TCAAAATGTA AACCTTGAAC GTTGTGAGCT TGCTAATTAC AAGCAATCTA TTCCTATGCC TCGCGGTGTG CACATGAACA TCGCTAAATA TATGCAATTG TGCCAGTATT TAAATACTTG CACATTAGCC GTGCCTGCCA ATATGCGTGT TATACATTTT GGCGCTGGTT CTGATAAAGG TATCGCTCCT GGTACCTCAG TTTTACGACA GTGGCTTCCT ACAGATGCCA TTATTATAGA TAATGATTTA AATGAGTTCG TGTCAGATGC TGACATAACT TTATTTGGAG ATTGTGTAAC TGTACGTGTC GGCCAACAAG TGGATCTTGT TATTTCCGAC ATGTATGATC CTACTACTAA GAATGTAACA GGTAGTAATG AGTCAAAGGC TTTATTCTTT ACTTACCTGT GTAACCTCAT TAATAATAAT CTTGCTCTTG GTGGGTCTGT TGCTATTAAA ATAACAGAAC ACTCTTGGAG CGTTGAACTT TATGAACTTA TGGGAAAATT TGCTTGGTGG ACTGTTTTCT GCACCAATGC AAATGCATCC TCATCTGAAG GATTCCTCTT AGGTATTAAT TACTTGGGTA CTATTAAAGA AAATATAGAT GGTGGTGCTA TGCACGCCAA CTATATATTT TGGAGAAATT CCACTCCTAT GAATCTGAGT ACTTACTCAC TTTTTGATTT ATCCAAGTTT CAATTAAAAT TAAAAGGAAC ACCAGTTCTT CAATTAAAGG AGAGTCAAAT TAACGAACTC GTAATATCTC TCCTGTCGCA GGGTAAGTTA CTTATCCGTG ACAATGATAC ACTCAGTGTT TCTACTGATG TTCTTGTTAA CACCTACAGA AAGTTACGT
Details for BecoA.18654.a.DG14.PD00324
PURIFICATION DATe: 6/18/2014
CONCENTRATION: 19.87mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSADW KPGHAMPSLF KVQNVNLERC ELANYKQSIP MPRGVHMNIA KYMQLCQYLN TCTLAVPANM RVIHFGAGSD KGIAPGTSVL RQWLPTDAII IDNDLNEFVS DADITLFGDC VTVRVGQQVD LVISDMYDPT TKNVTGSNES KALFFTYLCN LINNNLALGG SVAIKITEHS WSVELYELMG KFAWWTVFCT NANASSSEGF LLGINYLGTI KENIDGGAMH ANYIFWRNST PMNLSTYSLF DLSKFQLKLK GTPVLQLKES QINELVISLL SQGKLLIRDN DTLSVSTDV
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC GCTGACTGGA AACCGGGCCA CGCGATGCCG TCTCTGTTCA AAGTTCAGAA CGTAAACCTG GAACGCTGTG AACTGGCGAA CTATAAACAG TCTATCCCGA TGCCGCGTGG TGTACACATG AACATCGCGA AGTACATGCA GCTGTGCCAG TATCTTAACA CTTGCACTCT GGCTGTTCCG GCTAACATGC GTGTAATCCA CTTTGGCGCG GGCTCCGATA AAGGTATCGC ACCGGGCACT TCTGTACTGC GTCAGTGGCT GCCGACCGAT GCTATCATTA TCGACAACGA CCTGAACGAA TTTGTCTCTG ACGCTGACAT TACTCTGTTT GGTGACTGCG TAACTGTTCG TGTTGGCCAG CAAGTCGACC TGGTGATCAG CGACATGTAC GACCCGACTA CTAAGAACGT AACAGGTAGC AACGAAAGCA AAGCGCTGTT CTTCACTTAC CTGTGTAACT TAATCAACAA TAACCTGGCT CTGGGCGGCT CTGTCGCAAT TAAGATCACC GAACACTCCT GGTCTGTTGA ACTGTATGAA CTGATGGGCA AATTTGCTTG GTGGACTGTA TTTTGCACCA ACGCAAACGC GTCTTCCTCT GAAGGTTTCC TTCTTGGCAT CAACTACTTA GGCACTATCA AAGAAAACAT CGACGGCGGC GCGATGCACG CTAACTACAT CTTCTGGCGT AACTCCACTC CTATGAACCT GAGCACCTAC TCCTTGTTTG ACCTGTCCAA ATTTCAACTG AAACTGAAAG GTACTCCAGT TCTGCAGCTG AAAGAATCGC AGATTAACGA ACTGGTTATC TCTTTGCTGT CCCAGGGCAA ATTGCTGATC CGTGACAACG ACACCCTGTC CGTCTCCACC GACGTATGAT AAGCTTGGCT GTTTTGGCGG ATGAGAGAAG ATTTTCAGCC TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA ACAGAATTTG CCTGGCGGCA GTAGCGCGGT GGTCCCACCT GACCCCATGC CGAACTCAGA AGTGAAACGC CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCTACAAACT CTTTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTTGAACAA CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATAGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCAAGGAG ATGGCGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCTGC TCATGTTTGA CAGCTTATC