BecoA.18649.a

ORF1ab nsp1

CENTER ID: BecoA.18649.a
ORGANISM: Betacoronavirus Human betacoronavirus 2c EMC/2012
ASSOCIATED DISEASE: respiratory, GI infections
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BecoA.18649.a.DG11.GE00283 Full length( BecoA.18649.a ) 1 193
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BecoA.18649.a.DG11.PD00378 Full length( BecoA.18649.a ) 1 193
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|1235996.21.mat_peptide.1
UniProt: K0BWD0
ViPR: link
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFVAGVTAQ GARGTYRAAL NSEKHQDHVS LTVPLCGSGN LVEKLSPWFM DGENAYEVVK AMLLKKEPLL YVPIRLAGHT RHLPGPRVYL VERLIACENP FMVNQLAYSS SANGSLVGTT LQGKPIGMFF PYDIELVTGK QNILLRKYGR GGYHYTPFHY ERDNTSCPEW MDDFEADPKG KYAQNLLKKL IGG
NT Sequence
ATGTCTTTCG TGGCTGGTGT GACCGCGCAA GGTGCGCGCG GTACGTATCG AGCAGCGCTC AACTCTGAAA AACATCAAGA CCATGTGTCT CTAACTGTGC CACTCTGTGG TTCAGGAAAC CTGGTTGAAA AACTTTCACC ATGGTTCATG GATGGCGAAA ATGCCTATGA AGTGGTGAAG GCCATGTTAC TTAAAAAGGA GCCACTTCTC TATGTGCCCA TCCGGCTGGC TGGACACACT AGACACCTCC CAGGTCCTCG TGTGTACCTG GTTGAGAGGC TCATTGCTTG TGAAAATCCA TTCATGGTTA ACCAATTGGC TTATAGCTCT AGTGCAAATG GCAGCCTGGT TGGCACAACT TTGCAGGGCA AGCCTATTGG TATGTTCTTC CCTTATGACA TCGAACTTGT CACAGGAAAG CAAAATATTC TCCTGCGCAA GTATGGCCGT GGTGGTTATC ACTACACCCC ATTCCACTAT GAGCGAGACA ACACCTCTTG CCCTGAGTGG ATGGACGATT TTGAGGCGGA TCCTAAAGGC AAATATGCCC AGAATCTGCT TAAGAAGTTG ATTGGCGGT
Details for BecoA.18649.a.DG11.GE00283
HARVESTED ON: 7/23/2014
SEQUENCED ON: 9/30/2016
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSF VAGVTAQGAR GTYRAALNSE KHQDHVSLTV PLCGSGNLVE KLSPWFMDGE NAYEVVKAML LKKEPLLYVP IRLAGHTRHL PGPRVYLVER LIACENPFMV NQLAYSSSAN GSLVGTTLQG KPIGMFFPYD IELVTGKQNI LLRKYGRGGY HYTPFHYERD NTSCPEWMDD FEADXKGKYA QNLLKKLIGG
Validated NT Sequence
caaccaccga tcagtttctt cagcaggttc tgagcgtatt tacccttcnn atctgcctcg aaatcgtcca tccattcagg acaggaggtg ttgtcacgtt catagtggaa aggagtgtag tggtagccgc cgcgaccgta ctttctcagc aggatgttct gtttaccagt taccagctcg atatcgtacg ggaagaacat gcctatcggt ttaccttgca gagtggtacc caccagggag ccgtttgcgg aagaggagta tgccagctgg ttaaccatga acgggttttc gcaagcgatc agacgttcaa ccagataaac acgtgggccc ggcaggtgac gggtgtggcc ggccagacgg atcggaacgt acagcagtgg ttccttcttc agcagcatag ctttaacaac ttcgtatgcg ttttcaccgt ccataaacca cgggctcagt ttttcgacaa ggttgccaga accgcacagc gggacggtaa gggaaacgtg gtcctggtgc ttttcagagt tcagtgctgc tctgtaggtg ccacgtgcgc cctgtgcggt cacaccagca acgaaagaca tggatccacc gatttgttca cggtgtgctt cgataatgtc gttatcttcc atgtccagat cttccggagt ctggtctgcc tgaatacgga taccgtcgta cagaaagcgc agggaatcca tttccttgcc ctggcgttta gcgaatgctt ccatcaggcg acgcagcggg gtggtcttct taatcttgaa gaagatttcg ctgctaccgt cgctaacctt caggttgatg tgggtctccg gcttcacttc cggttttact tcgccgctat ggtggtggtg gtggtgggac at
Expected Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSF VAGVTAQGAR GTYRAALNSE KHQDHVSLTV PLCGSGNLVE KLSPWFMDGE NAYEVVKAML LKKEPLLYVP IRLAGHTRHL PGPRVYLVER LIACENPFMV NQLAYSSSAN GSLVGTTLQG KPIGMFFPYD IELVTGKQNI LLRKYGRGGY HYTPFHYERD NTSCPEWMDD FEADPKGKYA QNLLKKLIGG
Full NT Sequence (Expression Vector + Insert)
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggatcc atgtccttcg tagctggtgt gaccgcacag ggtgctagag gcacttatcg tgcagctttg aactccgaga agcaccagga tcacgtatcc ctgaccgtac cgctgtgcgg ttctggcaac ctggttgaaa aactgtctcc gtggtttatg gacggtgaga acgcatacga agttgttaaa gcaatgctgc tgaagaaaga accgctcctg tacgttccga tccgtttagc tggccatacc cgtcacctgc caggtccgcg tgtttacttg gttgaacgtc tgatcgcttg cgaaaaccct ttcatggtta accagctggc atattcctct tccgctaacg gctctttagt tggtaccacc ctgcagggca aaccgatcgg catgttcttc ccgtatgaca tcgaacttgt taccggtaag cagaacatcc ttctgcgtaa atacggtcgt ggcggctacc actacactcc gttccactac gaacgtgaca acacgtcctg ccctgaatgg atggacgact tcgaagctga cccgaaaggc aaatacgctc agaacctgct gaagaagctg atcggcggct gataagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaacttgaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaag gagatggcgc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tc
Details for BecoA.18649.a.DG11.PD00378
PURIFICATION DATe: 6/17/2015
CONCENTRATION: 4.022mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSF VAGVTAQGAR GTYRAALNSE KHQDHVSLTV PLCGSGNLVE KLSPWFMDGE NAYEVVKAML LKKEPLLYVP IRLAGHTRHL PGPRVYLVER LIACENPFMV NQLAYSSSAN GSLVGTTLQG KPIGMFFPYD IELVTGKQNI LLRKYGRGGY HYTPFHYERD NTSCPEWMDD FEADXKGKYA QNLLKKLIGG
Validated NT Sequence
caaccaccga tcagtttctt cagcaggttc tgagcgtatt tacccttcnn atctgcctcg aaatcgtcca tccattcagg acaggaggtg ttgtcacgtt catagtggaa aggagtgtag tggtagccgc cgcgaccgta ctttctcagc aggatgttct gtttaccagt taccagctcg atatcgtacg ggaagaacat gcctatcggt ttaccttgca gagtggtacc caccagggag ccgtttgcgg aagaggagta tgccagctgg ttaaccatga acgggttttc gcaagcgatc agacgttcaa ccagataaac acgtgggccc ggcaggtgac gggtgtggcc ggccagacgg atcggaacgt acagcagtgg ttccttcttc agcagcatag ctttaacaac ttcgtatgcg ttttcaccgt ccataaacca cgggctcagt ttttcgacaa ggttgccaga accgcacagc gggacggtaa gggaaacgtg gtcctggtgc ttttcagagt tcagtgctgc tctgtaggtg ccacgtgcgc cctgtgcggt cacaccagca acgaaagaca tggatccacc gatttgttca cggtgtgctt cgataatgtc gttatcttcc atgtccagat cttccggagt ctggtctgcc tgaatacgga taccgtcgta cagaaagcgc agggaatcca tttccttgcc ctggcgttta gcgaatgctt ccatcaggcg acgcagcggg gtggtcttct taatcttgaa gaagatttcg ctgctaccgt cgctaacctt caggttgatg tgggtctccg gcttcacttc cggttttact tcgccgctat ggtggtggtg gtggtgggac at
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSF VAGVTAQGAR GTYRAALNSE KHQDHVSLTV PLCGSGNLVE KLSPWFMDGE NAYEVVKAML LKKEPLLYVP IRLAGHTRHL PGPRVYLVER LIACENPFMV NQLAYSSSAN GSLVGTTLQG KPIGMFFPYD IELVTGKQNI LLRKYGRGGY HYTPFHYERD NTSCPEWMDD FEADPKGKYA QNLLKKLIGG
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC ATGTCCTTCG TAGCTGGTGT GACCGCACAG GGTGCTAGAG GCACTTATCG TGCAGCTTTG AACTCCGAGA AGCACCAGGA TCACGTATCC CTGACCGTAC CGCTGTGCGG TTCTGGCAAC CTGGTTGAAA AACTGTCTCC GTGGTTTATG GACGGTGAGA ACGCATACGA AGTTGTTAAA GCAATGCTGC TGAAGAAAGA ACCGCTCCTG TACGTTCCGA TCCGTTTAGC TGGCCATACC CGTCACCTGC CAGGTCCGCG TGTTTACTTG GTTGAACGTC TGATCGCTTG CGAAAACCCT TTCATGGTTA ACCAGCTGGC ATATTCCTCT TCCGCTAACG GCTCTTTAGT TGGTACCACC CTGCAGGGCA AACCGATCGG CATGTTCTTC CCGTATGACA TCGAACTTGT TACCGGTAAG CAGAACATCC TTCTGCGTAA ATACGGTCGT GGCGGCTACC ACTACACTCC GTTCCACTAC GAACGTGACA ACACGTCCTG CCCTGAATGG ATGGACGACT TCGAAGCTGA CCCGAAAGGC AAATACGCTC AGAACCTGCT GAAGAAGCTG ATCGGCGGCT GATAAGCTTG GCTGTTTTGG CGGATGAGAG AAGATTTTCA GCCTGATACA GATTAAATCA GAACGCAGAA GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC GGTGGTCCCA CCTGACCCCA TGCCGAACTC AGAAGTGAAA CGCCGTAGCG CCGATGGTAG TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC GTTTCTACAA ACTCTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTTGAA CAACACTCAA CCCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT AGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCAAG GAGATGGCGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC TGCTCATGTT TGACAGCTTA TC