BecoA.18647.a

ORF1ab nsp8 (putative primase)

CENTER ID:	BecoA.18647.a
ORGANISM:	Betacoronavirus Human betacoronavirus 2c EMC/2012
ASSOCIATED DISEASE:	respiratory, GI infections
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BecoA.18647.a.DG11.PD00329	Full length( BecoA.18647.a )		1	200

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1235996.21.mat_peptide.8
UniProt:	K0BWD0
ViPR:	link

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

ATLSEFSHLA TFAELEAAQK AYQEAMDSGD TSPQVLKALQ KAVNIAKNAY EKDKAVARKL ERMADQAMTS MYKQARAEDK KAKIVSAMQT MLFGMIKKLD NDVLNGIISN ARNGCIPLSV IPLCASNKLR VVIPDFTVWN QVVTYPSLNY AGALWDITVI NNVDNEIVKS SDVVDSNENL TWPLVLECTR ASTSAVKLQ

NT Sequence

GCTACTCTTT CTGAGTTTTC ACACTTAGCT ACCTTTGCTG AGTTGGAAGC TGCGCAGAAA GCCTATCAGG AAGCTATGGA CTCTGGTGAC ACCTCACCAC AAGTTCTTAA GGCTTTGCAG AAGGCTGTTA ATATAGCTAA AAACGCCTAT GAGAAGGATA AGGCAGTGGC CCGTAAGTTA GAACGTATGG CTGATCAGGC TATGACTTCT ATGTATAAGC AAGCACGTGC TGAAGACAAG AAAGCAAAAA TTGTCAGTGC TATGCAAACT ATGTTGTTTG GTATGATTAA GAAGCTCGAC AACGATGTTC TTAATGGTAT CATTTCTAAC GCTAGGAATG GTTGTATACC TCTTAGTGTC ATCCCACTGT GTGCTTCAAA TAAACTTCGC GTTGTAATTC CTGACTTCAC CGTCTGGAAT CAGGTAGTCA CATATCCCTC GCTTAACTAC GCTGGGGCTT TGTGGGACAT TACAGTTATA AACAATGTGG ACAATGAAAT TGTTAAGTCT TCAGATGTTG TAGACAGCAA TGAAAATTTA ACATGGCCAC TTGTTTTAGA ATGCACTAGG GCATCCACTT CTGCCGTTAA GTTGCAA

Details for BecoA.18647.a.DG11.PD00329

PURIFICATION DATe:	7/6/2014
CONCENTRATION:	20.67mg/ml
OBSERVED MW:	32kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	100µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSATL SEFSHLATFA ELEAAQKAYQ EAMDSGDTSP QVLKALQKAV NIAKNAYEKD KAVARKLERM ADQAMTSMYK QARAEDKKAK IVSAMQTMLF GMIKKLDNDV LNGIISNARN GCIPLSVIPL CASNKLRVVI PDFTVWNQVV TYPSLNYAGA LWDITVINNV DNEIVKSSDV VDSNENLTWP LVLECTRAST SAVKLQ

Full NT Sequence (Expression Vector + Insert)

ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC GCGACACTGT CCGAATTTTC TCATCTGGCA ACTTTCGCCG AATTGGAGGC TGCACAGAAG GCGTATCAAG AAGCAATGGA TAGCGGCGAT ACCTCTCCTC AGGTTCTGAA GGCTCTGCAG AAAGCAGTAA ATATTGCTAA GAACGCGTAC GAGAAGGATA AAGCAGTTGC ACGTAAACTC GAACGTATGG CTGACCAAGC TATGACCTCT ATGTACAAAC AAGCGCGTGC TGAAGACAAG AAGGCTAAAA TCGTTTCAGC TATGCAAACT ATGCTCTTCG GTATGATCAA GAAGCTCGAC AACGACGTTC TGAACGGCAT CATCAGCAAC GCTCGTAACG GCTGCATCCC GCTTAGCGTA ATTCCACTGT GCGCGTCTAA CAAACTGCGC GTCGTTATCC CGGACTTCAC TGTTTGGAAC CAGGTAGTTA CTTACCCTAG CCTGAACTAC GCGGGTGCAC TGTGGGACAT CACTGTTATC AACAACGTAG ATAACGAGAT CGTTAAATCT TCCGACGTAG TAGACTCTAA CGAGAATCTG ACCTGGCCGC TTGTTCTGGA ATGCACCCGT GCTTCCACAT CCGCTGTTAA GCTGCAGTGA TAAGCTTGGC TGTTTTGGCG GATGAGAGAA GATTTTCAGC CTGATACAGA TTAAATCAGA ACGCAGAAGC GGTCTGATAA AACAGAATTT GCCTGGCGGC AGTAGCGCGG TGGTCCCACC TGACCCCATG CCGAACTCAG AAGTGAAACG CCGTAGCGCC GATGGTAGTG TGGGGTCTCC CCATGCGAGA GTAGGGAACT GCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA TTTGAACGTT GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC CATAAACTGC CAGGCATCAA ATTAAGCAGA AGGCCATCCT GACGGATGGC CTTTTTGCGT TTCTACAAAC TCTTTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTTGAACA ACACTCAACC CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCAAGGA GATGGCGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCTG CTCATGTTTG ACAGCTTATC