BatrA.18414.a

TrwJ2 protein (Trw type IV secretion machinery)

CENTER ID: BatrA.18414.a
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.18414.a.B2.GE35716 mature protein 23 310
BatrA.18414.a.HZ2.GE41793 mature protein 23 310
BatrA.18414.a.MB2.GE41792 mature protein 23 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|382640.5.peg.2561
RefSeq: YP_001610480.1
UniProt: A9IZ48

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKQFIIIGV ISLLEMMNLA HANNAGFHNR DTYDRTQGRL MEGGKYRKYP GLSDSTHNRD VTITKRPNRP RVKKKPAPPK RSRPAHPPQP TPLPQPTPLP QPAQPPQPAL SKEYSEILQL LKKGEIIELL KKQLDSKRQQ LSQVEKAYQA ITKSQKTNPK QIDFSSFFLK EPEFLYKDSK LSRQSYQEVL EEENKVSGNF DQISKAIFGR LQSVSVQDKA VSLESFENVK SRFQYLRGLL DELQTKEKLK DIADLQAHID GTLAMIQNEF IKMEMVAYLH DAEQSLIKRK RRELDMMFFD HEKNQMPIIR
NT Sequence
atgaaaaagc aatttattat aataggagtg attagccttt tggaaatgat gaatttggcg catgcaaata acgcaggttt tcataataga gatacttatg atagaactca aggaaggtta atggaaggag gtaaatatcg gaaatatcct ggcttgtcgg attcaacaca caatagagat gttactataa ctaaacgtcc taatagacca agagttaaaa aaaagccagc accacctaaa aggtcacgac ctgctcatcc accacaacct actccattgc cacaacctac tccattgcca caacctgctc aaccgccaca acctgctcta tcaaaagaat attcggaaat ccttcagctt ttaaaaaaag gggaaattat tgagctctta aaaaaacagc ttgactcgaa aagacaacaa ctttcacaag tagaaaaagc ttatcaggct ataacaaaga gtcagaaaac aaacccaaag caaatagact tttctagttt ttttcttaaa gagccagaat ttctctataa agatagtaag ctttcaaggc aatcatatca agaggtcttg gaggaagaaa acaaagtttc tgggaatttt gatcaaatca gcaaagctat ttttgggcgt ttacaatctg tatcggttca agataaagct gtgagtttgg aaagttttga aaacgtaaaa agtcgttttc aatacctcag aggactactc gatgagcttc aaacaaaaga aaaactgaaa gacattgcag accttcaagc acatatcgat ggtacattag ctatgatcca aaatgaattt ataaaaatgg aaatggtagc gtatttacat gacgctgagc aatcacttat caaaaggaaa agacgcgaac ttgatatgat gttttttgac catgaaaaaa accagatgcc gatcatacgg tga
Details for BatrA.18414.a.B2.GE35716
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/3/2012
EXPECTED MW: 34kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN NAGFHNRDTY DRTQGRLMEG GKYRKYPGLS DSTHNRDVTI TKRPNRPRVK KKPAPPKRSR PAHPPQPTPL PQPTPLPQPA QPPQPALSKE YSEILQLLKK GEIIELLKKQ LDSKRQQLSQ VEKAYQAITK SQKTNPKQID FSSFFLKEPE FLYKDSKLSR QSYQEVLEEE NKVSGNFDQI SKAIFGRLQS VSVQDKAVSL ESFENVKSRF QYLRGLLDEL QTKEKLKDIA DLQAHIDGTL AMIQNEFIKM EMVAYLHDAE QSLIKRKRRE LDMMFFDHEK NQMPIIR
Validated NT Sequence
gnncctatct tactcactta ccgtatgatc ggcatctggt ttttttcatg gtcaaaaaac atcatatcaa gttcgcgtct tttccttttg ataagtgatt gctcagcgtc atgtaaatac gctaccattt ccatttttat aaattcattt tggatcatag ctaatgtacc atcgatatgt gcttgaaggt ctgcaatgtc tttcagtttt tcttttgttt gaagctcatc gagtagtcct ctgaggtatt gaaaacgact ttttacgttt tcaaaacttt ccaaactcac agctttatct tgaaccgata cagattgtaa acgcccaaaa atagctttgc tgatttgatc aaaattccca gaaactttgt tttcttcctc caagacctct tgatatgatt gccttgaaag cttactatct ttatagagaa attctggctc tttaagaaaa aaactagaaa agtctatttg ctttgggttt gttttctgac tctttgttat agcctgataa gctttttcta cttgtgaaag ttgttgtctt ttcgagtcaa gctgtttttt taagagctca ataatttccc ctttttttaa aagctgaagg atttccgaat attcttttga tagagcaggt tgtggcggtt gagcaggttg tggcaatgga gtaggttgtg gcaatggagt aggttgtggt ggatgagcag gtcgtgacct tttaggtggt gctggctttt ttttaactct tggtctatta ggacgtttag ttatagtaac atctctattg tgtgttgaat ccgacaagcc aggatatttc cgatatttac ctccttccat taaccttcct tgagttctat cataagtatc tctattatga aaacctgcgt tattcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMN NAGFHNRDTY DRTQGRLMEG GKYRKYPGLS DSTHNRDVTI TKRPNRPRVK KKPAPPKRSR PAHPPQPTPL PQPTPLPQPA QPPQPALSKE YSEILQLLKK GEIIELLKKQ LDSKRQQLSQ VEKAYQAITK SQKTNPKQID FSSFFLKEPE FLYKDSKLSR QSYQEVLEEE NKVSGNFDQI SKAIFGRLQS VSVQDKAVSL ESFENVKSRF QYLRGLLDEL QTKEKLKDIA DLQAHIDGTL AMIQNEFIKM EMVAYLHDAE QSLIKRKRRE LDMMFFDHEK NQMPIIR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata acgcaggttt tcataataga gatacttatg atagaactca aggaaggtta atggaaggag gtaaatatcg gaaatatcct ggcttgtcgg attcaacaca caatagagat gttactataa ctaaacgtcc taatagacca agagttaaaa aaaagccagc accacctaaa aggtcacgac ctgctcatcc accacaacct actccattgc cacaacctac tccattgcca caacctgctc aaccgccaca acctgctcta tcaaaagaat attcggaaat ccttcagctt ttaaaaaaag gggaaattat tgagctctta aaaaaacagc ttgactcgaa aagacaacaa ctttcacaag tagaaaaagc ttatcaggct ataacaaaga gtcagaaaac aaacccaaag caaatagact tttctagttt ttttcttaaa gagccagaat ttctctataa agatagtaag ctttcaaggc aatcatatca agaggtcttg gaggaagaaa acaaagtttc tgggaatttt gatcaaatca gcaaagctat ttttgggcgt ttacaatctg tatcggttca agataaagct gtgagtttgg aaagttttga aaacgtaaaa agtcgttttc aatacctcag aggactactc gatgagcttc aaacaaaaga aaaactgaaa gacattgcag accttcaagc acatatcgat ggtacattag ctatgatcca aaatgaattt ataaaaatgg aaatggtagc gtatttacat gacgctgagc aatcacttat caaaaggaaa agacgcgaac ttgatatgat gttttttgac catgaaaaaa accagatgcc gatcatacgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BatrA.18414.a.HZ2.GE41793
HARVESTED ON: 8/7/2017
SEQUENCED ON: 9/20/2017
EXPECTED MW: 47kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 87
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLXXLYDGIR XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXQXXXXXX XXXXXXRDTY DRTQGXXMEG GKXXKYPGXX DSTHNRDVTI TKRPNRPRVK KKPAPPKRSR PAHPPQPTPL PQPTPLPQPA QPPQPALSKE YSEILQLLKK GEIIELLKKQ LDSKRQQLSQ VEKAYQAITK SQKTNPKQID FSSFFLKEPE FLYKDSKLSR QSYQEVLEEE NKVSGNFDQI SKAIFGRLQS VSVQDKAVSL ESFENVKSRF QYLRGLLDEL QTKEKLKDIA DLQAHIDGTL AMIQNEFIKM EMVAYLHDAE QSLIKRKRRE LDMMFFDHEK NQMPIIR
Validated NT Sequence
ttaccgtatg atcggcatct ggtttttttc atggtcaaaa aacatcatat caagttcgcg tcttttcctt ttgataagtg attgctcagc gtcatgtaaa tacgctacca tttccatttt tataaattca ttttggatca tagctaatgt accatcgata tgtgcttgaa ggtctgcaat gtctttcagt ttttcttttg tttgaagctc atcgagtagt cctctgaggt attgaaaacg actttttacg ttttcaaaac tttccaaact cacagcttta tcttgaaccg atacagattg taaacgccca aaaatagctt tgctgatttg atcaaaattc ccagaaactt tgttttcttc ctccaagacc tcttgatatg attgccttga aagcttacta tctttataga gaaattctgg ctctttaaga aaaaaactag aaaagtctat ttgctttggg tttgttttct gactctttgt tatagcctga taagcttttt ctacttgtga aagttgttgt cttttcgagt caagctgttt ttttaagagc tcaataattt cccctttttt taaaagctga aggatttccg aatattcttt tgatagagca ggttgtggcg gttgagcagg ttgtggcaat ggagtaggtt gtggcaatgg agtaggttgt ggtggatgag caggtcgtga ccttttaggt ggtgctggct tttttttaac tcttggtcta ttaggacgtt tagttatagt aacatctcta ttgtgtgttg aatcnnnnnn gccaggatat ttccnnnntt tacctccttc cattnnnntt ccttgagttc tatcataagt atctctatnn nnnananntn nnnnnnnnnn nnnnnnnnnn ncctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntnnn nnncnnnnnn tncnnnnntc tnntcannnn nnnttctaat accgtcgtac aagnnnctta aggagtccat ttccttaccc tgtcttttag cgaacgcttc catcagcctt cttaaaggag tggtcttttt gatcttgaag aagatctctg aagatccatc ggacaccttt aaattgatgt gagtctcagg cttgacttct ggcttgacct ctggcttagc ttcttgattg acttctgagt ccgacttcat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSNN AGFHNRDTYD RTQGRLMEGG KYRKYPGLSD STHNRDVTIT KRPNRPRVKK KPAPPKRSRP AHPPQPTPLP QPTPLPQPAQ PPQPALSKEY SEILQLLKKG EIIELLKKQL DSKRQQLSQV EKAYQAITKS QKTNPKQIDF SSFFLKEPEF LYKDSKLSRQ SYQEVLEEEN KVSGNFDQIS KAIFGRLQSV SVQDKAVSLE SFENVKSRFQ YLRGLLDELQ TKEKLKDIAD LQAHIDGTLA MIQNEFIKME MVAYLHDAEQ SLIKRKRREL DMMFFDHEKN QMPIIR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga ataacgcagg ttttcataat agagatactt atgatagaac tcaaggaagg ttaatggaag gaggtaaata tcggaaatat cctggcttgt cggattcaac acacaataga gatgttacta taactaaacg tcctaataga ccaagagtta aaaaaaagcc agcaccacct aaaaggtcac gacctgctca tccaccacaa cctactccat tgccacaacc tactccattg ccacaacctg ctcaaccgcc acaacctgct ctatcaaaag aatattcgga aatccttcag cttttaaaaa aaggggaaat tattgagctc ttaaaaaaac agcttgactc gaaaagacaa caactttcac aagtagaaaa agcttatcag gctataacaa agagtcagaa aacaaaccca aagcaaatag acttttctag tttttttctt aaagagccag aatttctcta taaagatagt aagctttcaa ggcaatcata tcaagaggtc ttggaggaag aaaacaaagt ttctgggaat tttgatcaaa tcagcaaagc tatttttggg cgtttacaat ctgtatcggt tcaagataaa gctgtgagtt tggaaagttt tgaaaacgta aaaagtcgtt ttcaatacct cagaggacta ctcgatgagc ttcaaacaaa agaaaaactg aaagacattg cagaccttca agcacatatc gatggtacat tagctatgat ccaaaatgaa tttataaaaa tggaaatggt agcgtattta catgacgctg agcaatcact tatcaaaagg aaaagacgcg aacttgatat gatgtttttt gaccatgaaa aaaaccagat gccgatcata cggtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BatrA.18414.a.MB2.GE41792
HARVESTED ON: data unavailable
SEQUENCED ON: 9/20/2017
EXPECTED MW: 78kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS NNAGFHNRDT YDRTQGRLME GGKYRKYPGL SDSTHNRDVT ITKRPNRPRV KKKPAPPKRS RPAHPPQPTP LPQPTPLPQP AQPPQPALSK EYSEILQLLK KGEIIELLKK QLDSKRQQLS QVEKAYQAIT KSQKTNPKQI DFSSFFLKEP EFLYKDSKLS RQSYQEVLEE ENKVSGNFDQ ISKAIFGRLQ SVSVQDKAVS LESFENVKSR FQYLRGLLDE LQTKEKLKDI ADLQAHIDGT LAMIQNEFIK MEMVAYLHDA EQSLIKRKRR ELDMMFFDHE KNQMPIIR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgaataa cgcaggtttt cataatagag atacttatga tagaactcaa ggaaggttaa tggaaggagg taaatatcgg aaatatcctg gcttgtcgga ttcaacacac aatagagatg ttactataac taaacgtcct aatagaccaa gagttaaaaa aaagccagca ccacctaaaa ggtcacgacc tgctcatcca ccacaaccta ctccattgcc acaacctact ccattgccac aacctgctca accgccacaa cctgctctat caaaagaata ttcggaaatc cttcagcttt taaaaaaagg ggaaattatt gagctcttaa aaaaacagct tgactcgaaa agacaacaac tttcacaagt agaaaaagct tatcaggcta taacaaagag tcagaaaaca aacccaaagc aaatagactt ttctagtttt tttcttaaag agccagaatt tctctataaa gatagtaagc tttcaaggca atcatatcaa gaggtcttgg aggaagaaaa caaagtttct gggaattttg atcaaatcag caaagctatt tttgggcgtt tacaatctgt atcggttcaa gataaagctg tgagtttgga aagttttgaa aacgtaaaaa gtcgttttca atacctcaga ggactactcg atgagcttca aacaaaagaa aaactgaaag acattgcaga ccttcaagca catatcgatg gtacattagc tatgatccaa aatgaattta taaaaatgga aatggtagcg tatttacatg acgctgagca atcacttatc aaaaggaaaa gacgcgaact tgatatgatg ttttttgacc atgaaaaaaa ccagatgccg atcatacggt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa