BatrA.18413.a

Putative uncharacterized protein (Trw type IV secretion machinery)

CENTER ID: BatrA.18413.a
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.18413.a.B2.GE35715 mature protein 21 278
BatrA.18413.a.B4.GE41838 DUF1849_short 29 262
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.18413.a.B2.PW37262 mature protein 21 278

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|382640.5.peg.944
RefSeq: YP_001609304.1
UniProt: A9ISJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRALFIIIL YIVFFCTVRA EEPIFIAPHR AIYDFHLDSV SHEMTISGMS GRMVYELTGS ACQGYSTRFR FISRLHVEDM PVRLTDQQTT SYETGDSRTF RFNITDQEGE EVAHRAEGVA ERTEDGITVT LKTPKEKEYK LAMAEFPMMQ LKNIIRHAKA GDHFYNATVF DGTNKADKVI KESIAIGEKK ALLSDSETEK MRKLDEEEYW PVTISYFDDS ENKDGLPVYR SSFLLHENGV MRDLHIDYGT FSVRAKLNSL EFLELGKDLG QCENANIK
NT Sequence
atgattagag cattatttat aataatttta tatattgttt ttttctgtac agtgagagct gaagagccta tttttattgc acctcatcga gcaatttatg attttcatct cgatagtgtt tctcatgaaa tgacaatttc tgggatgtca gggcgaatgg tttacgagct tactggttcg gcatgccaag gctatagcac acgttttcgt ttcattagcc gtcttcatgt tgaagatatg ccggtacgtt taacggatca acagacgacg agttatgaaa caggagatag tcgtacattt cgttttaata ttaccgatca agagggagaa gaggttgcac atcgtgccga aggggtcgcg gagcgaacgg aagatggtat tacggttacc ttaaagacgc caaaggaaaa agaatataag ctcgcgatgg cagaatttcc aatgatgcaa ctaaagaata ttatccgcca tgcaaaagcg ggggatcatt tttataacgc tactgtattt gatgggacaa ataaagcaga taaagttata aaagaaagta tcgccatagg ggaaaagaag gcgcttttat ctgacagtga gacggaaaaa atgagaaaat tggatgaaga agaatattgg cctgttacaa tctcttattt tgatgatagt gaaaataaag atggcttgcc ggtttatcga agcagttttc tcttacatga gaatggtgtc atgcgtgatc ttcatataga ttatggaact ttttcagtac gtgcaaaatt gaacagcctt gaattcctcg aacttgggaa agatcttggt caatgtgaaa atgcaaatat taaatga
Details for BatrA.18413.a.B2.GE35715
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 30kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME EPIFIAPHRA IYDFHLDSVS HEMTISGMSG RMVYELTGSA CQGYSTRFRF ISRLHVEDMP VRLTDQQTTS YETGDSRTFR FNITDQEGEE VAHRAEGVAE RTEDGITVTL KTPKEKEYKL AMAEFPMMQL KNIIRHAKAG DHFYNATVFD GTNKADKVIK ESIAIGEKKA LLSDSETEKM RKLDEEEYWP VTISYFDDSE NKDGLPVYRS SFLLHENGVM RDLHIDYGTF SVRAKLNSLE FLELGKDLGQ CENANIK
Validated NT Sequence
tttnnnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatggaaga gcctattttt attgcacctc atcgagcaat ttatgatttt catctcgata gtgtttctca tgaaatgaca atttctggga tgtcagggcg aatggtttac gagcttactg gttcggcatg ccaaggctat agcacacgtt ttcgtttcat tagccgtctt catgttgaag atatgccggt acgtttaacg gatcaacaga cgacgagtta tgaaacagga gatagtcgta catttcgttt taatattacc gatcaagagg gagaagaggt tgcacatcgt gccgaagggg tcgcggagcg aacggaagat ggtattacgg ttaccttaaa gacgccaaag gaaaaagaat ataagctcgc gatggcagaa tttccaatga tgcaactaaa gaatattatc cgccatgcaa aagcggggga tcatttttat aacgctactg tatttgatgg gacaaataaa gcagataaag ttataaaaga aagtatcgcc ataggggaaa agaaggcgct tttatctgac agtgagacgg aaaaaatgag aaaattggat gaagaagaat attggcctgt tacaatctct tattttgatg atagtgaaaa taaagatggc ttgccggttt atcgaagcag ttttctctta catgagaatg gtgtcatgcg tgatcttcat atagattatg gaactttttc agtacgtgca aaattgaaca gccttgaatt cctcgaactt gggaaagatc ttggtcaatg tgaaaatgca aatattaaat aantgagtaa natnnnnnnn nnnnnnnccn nnnnnnnnnn nnnnnnnngn ggnnnnnnna naaaannnnn nnnnnnnnnn tnntnttnnn nnnnnnnngn nnnnnnnnnn gtga
Expected Protein Sequence
MAHHHHHHME EPIFIAPHRA IYDFHLDSVS HEMTISGMSG RMVYELTGSA CQGYSTRFRF ISRLHVEDMP VRLTDQQTTS YETGDSRTFR FNITDQEGEE VAHRAEGVAE RTEDGITVTL KTPKEKEYKL AMAEFPMMQL KNIIRHAKAG DHFYNATVFD GTNKADKVIK ESIAIGEKKA LLSDSETEKM RKLDEEEYWP VTISYFDDSE NKDGLPVYRS SFLLHENGVM RDLHIDYGTF SVRAKLNSLE FLELGKDLGQ CENANIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag agcctatttt tattgcacct catcgagcaa tttatgattt tcatctcgat agtgtttctc atgaaatgac aatttctggg atgtcagggc gaatggttta cgagcttact ggttcggcat gccaaggcta tagcacacgt tttcgtttca ttagccgtct tcatgttgaa gatatgccgg tacgtttaac ggatcaacag acgacgagtt atgaaacagg agatagtcgt acatttcgtt ttaatattac cgatcaagag ggagaagagg ttgcacatcg tgccgaaggg gtcgcggagc gaacggaaga tggtattacg gttaccttaa agacgccaaa ggaaaaagaa tataagctcg cgatggcaga atttccaatg atgcaactaa agaatattat ccgccatgca aaagcggggg atcattttta taacgctact gtatttgatg ggacaaataa agcagataaa gttataaaag aaagtatcgc cataggggaa aagaaggcgc ttttatctga cagtgagacg gaaaaaatga gaaaattgga tgaagaagaa tattggcctg ttacaatctc ttattttgat gatagtgaaa ataaagatgg cttgccggtt tatcgaagca gttttctctt acatgagaat ggtgtcatgc gtgatcttca tatagattat ggaacttttt cagtacgtgc aaaattgaac agccttgaat tcctcgaact tgggaaagat cttggtcaat gtgaaaatgc aaatattaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BatrA.18413.a.B4.GE41838
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMH RAIYDFHLDS VSHEMTISGM SGRMVYELTG SACQGYSTRF RFISRLHVED MPVRLTDQQT TSYETGDSRT FRFNITDQEG EEVAHRAEGV AERTEDGITV TLKTPKEKEY KLAMAEFPMM QLKNIIRHAK AGDHFYNATV FDGTNKADKV IKESIAIGEK KALLSDSETE KMRKLDEEEY WPVTISYFDD SENKDGLPVY RSSFLLHENG VMRDLHIDYG TFSVRAXLNT LNSK
Validated NT Sequence
atcttactca cttagaattc aaggtgttca atnttgcacg tactgaaaaa gttccataat ctatatgaag atcacgcatg acaccattct catgtaagag aaaactgctt cgataaaccg gcaagccatc tttattttca ctatcatcaa aataagagat tgtaacaggc caatattctt cttcatccaa ttttctcatt ttttccgtct cactgtcaga taaaagcgcc ttcttttccc ctatggcgat actttctttt ataactttat ctgctttatt tgtcccatca aatacagtag cgttataaaa atgatccccc gcttttgcat ggcggataat attctttagt tgcatcattg gaaattctgc catcgcgagc ttatattctt tttcctttgg cgtctttaag gtaaccgtaa taccatcttc cgttcgctcc gcgacccctt cggcacgatg tgcaacctct tctccctctt gatcggtaat attaaaacga aatgtacgac tatctcctgt ttcataactc gtcgtctgtt gatccgttaa acgtaccggc atatcttcaa catgaagacg gctaatgaaa cgaaaacgtg tgctatagcc ttggcatgcc gaaccagtaa gctcgtaaac cattcgccct gacatcccag aaattgtcat ttcatgagaa acactatcga gatgaaaatc ataaattgct cgatgcatat ggtggtggtg gtggtgagcc atggtata
Expected Protein Sequence
MAHHHHHHHR AIYDFHLDSV SHEMTISGMS GRMVYELTGS ACQGYSTRFR FISRLHVEDM PVRLTDQQTT SYETGDSRTF RFNITDQEGE EVAHRAEGVA ERTEDGITVT LKTPKEKEYK LAMAEFPMMQ LKNIIRHAKA GDHFYNATVF DGTNKADKVI KESIAIGEKK ALLSDSETEK MRKLDEEEYW PVTISYFDDS ENKDGLPVYR SSFLLHENGV MRDLHIDYGT FSVRAKLNSL EF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcatcgag caatttatga ttttcatctc gatagtgttt ctcatgaaat gacaatttct gggatgtcag ggcgaatggt ttacgagctt actggttcgg catgccaagg ctatagcaca cgttttcgtt tcattagccg tcttcatgtt gaagatatgc cggtacgttt aacggatcaa cagacgacga gttatgaaac aggagatagt cgtacatttc gttttaatat taccgatcaa gagggagaag aggttgcaca tcgtgccgaa ggggtcgcgg agcgaacgga agatggtatt acggttacct taaagacgcc aaaggaaaaa gaatataagc tcgcgatggc agaatttcca atgatgcaac taaagaatat tatccgccat gcaaaagcgg gggatcattt ttataacgct actgtatttg atgggacaaa taaagcagat aaagttataa aagaaagtat cgccataggg gaaaagaagg cgcttttatc tgacagtgag acggaaaaaa tgagaaaatt ggatgaagaa gaatattggc ctgttacaat ctcttatttt gatgatagtg aaaataaaga tggcttgccg gtttatcgaa gcagttttct cttacatgag aatggtgtca tgcgtgatct tcatatagat tatggaactt tttcagtacg tgcaaaattg aacagccttg aattctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BatrA.18413.a.B2.PW37262
PURIFICATION DATe: 3/21/2013
CONCENTRATION: 15mg/ml
OBSERVED MW: 31kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME EPIFIAPHRA IYDFHLDSVS HEMTISGMSG RMVYELTGSA CQGYSTRFRF ISRLHVEDMP VRLTDQQTTS YETGDSRTFR FNITDQEGEE VAHRAEGVAE RTEDGITVTL KTPKEKEYKL AMAEFPMMQL KNIIRHAKAG DHFYNATVFD GTNKADKVIK ESIAIGEKKA LLSDSETEKM RKLDEEEYWP VTISYFDDSE NKDGLPVYRS SFLLHENGVM RDLHIDYGTF SVRAKLNSLE FLELGKDLGQ CENANIK
Validated NT Sequence
tttnnnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatggaaga gcctattttt attgcacctc atcgagcaat ttatgatttt catctcgata gtgtttctca tgaaatgaca atttctggga tgtcagggcg aatggtttac gagcttactg gttcggcatg ccaaggctat agcacacgtt ttcgtttcat tagccgtctt catgttgaag atatgccggt acgtttaacg gatcaacaga cgacgagtta tgaaacagga gatagtcgta catttcgttt taatattacc gatcaagagg gagaagaggt tgcacatcgt gccgaagggg tcgcggagcg aacggaagat ggtattacgg ttaccttaaa gacgccaaag gaaaaagaat ataagctcgc gatggcagaa tttccaatga tgcaactaaa gaatattatc cgccatgcaa aagcggggga tcatttttat aacgctactg tatttgatgg gacaaataaa gcagataaag ttataaaaga aagtatcgcc ataggggaaa agaaggcgct tttatctgac agtgagacgg aaaaaatgag aaaattggat gaagaagaat attggcctgt tacaatctct tattttgatg atagtgaaaa taaagatggc ttgccggttt atcgaagcag ttttctctta catgagaatg gtgtcatgcg tgatcttcat atagattatg gaactttttc agtacgtgca aaattgaaca gccttgaatt cctcgaactt gggaaagatc ttggtcaatg tgaaaatgca aatattaaat aantgagtaa natnnnnnnn nnnnnnnccn nnnnnnnnnn nnnnnnnngn ggnnnnnnna naaaannnnn nnnnnnnnnn tnntnttnnn nnnnnnnngn nnnnnnnnnn gtga
Expressed Protein Sequence
MAHHHHHHME EPIFIAPHRA IYDFHLDSVS HEMTISGMSG RMVYELTGSA CQGYSTRFRF ISRLHVEDMP VRLTDQQTTS YETGDSRTFR FNITDQEGEE VAHRAEGVAE RTEDGITVTL KTPKEKEYKL AMAEFPMMQL KNIIRHAKAG DHFYNATVFD GTNKADKVIK ESIAIGEKKA LLSDSETEKM RKLDEEEYWP VTISYFDDSE NKDGLPVYRS SFLLHENGVM RDLHIDYGTF SVRAKLNSLE FLELGKDLGQ CENANIK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAG AGCCTATTTT TATTGCACCT CATCGAGCAA TTTATGATTT TCATCTCGAT AGTGTTTCTC ATGAAATGAC AATTTCTGGG ATGTCAGGGC GAATGGTTTA CGAGCTTACT GGTTCGGCAT GCCAAGGCTA TAGCACACGT TTTCGTTTCA TTAGCCGTCT TCATGTTGAA GATATGCCGG TACGTTTAAC GGATCAACAG ACGACGAGTT ATGAAACAGG AGATAGTCGT ACATTTCGTT TTAATATTAC CGATCAAGAG GGAGAAGAGG TTGCACATCG TGCCGAAGGG GTCGCGGAGC GAACGGAAGA TGGTATTACG GTTACCTTAA AGACGCCAAA GGAAAAAGAA TATAAGCTCG CGATGGCAGA ATTTCCAATG ATGCAACTAA AGAATATTAT CCGCCATGCA AAAGCGGGGG ATCATTTTTA TAACGCTACT GTATTTGATG GGACAAATAA AGCAGATAAA GTTATAAAAG AAAGTATCGC CATAGGGGAA AAGAAGGCGC TTTTATCTGA CAGTGAGACG GAAAAAATGA GAAAATTGGA TGAAGAAGAA TATTGGCCTG TTACAATCTC TTATTTTGAT GATAGTGAAA ATAAAGATGG CTTGCCGGTT TATCGAAGCA GTTTTCTCTT ACATGAGAAT GGTGTCATGC GTGATCTTCA TATAGATTAT GGAACTTTTT CAGTACGTGC AAAATTGAAC AGCCTTGAAT TCCTCGAACT TGGGAAAGAT CTTGGTCAAT GTGAAAATGC AAATATTAAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT