BatrA.18411.a

VirB10 homologue (VirB type IV secretion machinery)

CENTER ID: BatrA.18411.a
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.18411.a.B2.GE35764 soluble domain 47 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|85701.4.peg.1798
RefSeq: WP_012232123.1
UniProt: Q8GJ58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNNVDEKNI NDRDTIKNAQ GKSQQSNVGK AIALVILFSV CLYLAYSTLV TDKKQPIESS KEASKEGKVI KQTELFRPAQ PAPPSVEPAH RKNALLPKVE LPTPNINQLN SDDPLLEAAQ RAPVLAYANT QQGKTNSQAN NGTLPHQLES KPDETTQRFN HLLKPTVLEG IRASTLGNRN YIITMGTSIP CILETAINSD QQGFASCIVS RDILSDNGRV VLLDKGTQIV GEYRAGLKKG QKRLFVLWNR AKTPNGIIIS LASPATDSLG RSGIDGDIDN HWLERIGSAL LVSLIKDATS YANKRLSKKQ DKEENETISS GQNIANIIVE NYANIPPTLS KNQGEMVNVF VARDLDFSSV YKLKVIENKK QIINRAVSRN FYKNSAIPLK
NT Sequence
atgaataaca acgtggatga aaaaaatatt aacgatcgcg atacaataaa aaatgctcaa ggaaaaagtc aacaaagcaa tgtaggcaaa gcaatcgctc ttgttattct tttcagtgtc tgtctttatt tagcctattc aacgcttgtt acagacaaaa aacaaccgat tgaatcttca aaagaagcat caaaagaagg taaagttatt aaacaaacag aactttttcg tcctgcccag cctgctcctc cctccgttga accggctcac agaaagaacg ctctgttacc caaagtcgag ctaccaaccc ccaacatcaa tcaattaaat tctgatgatc ccctgttgga agcagcacaa cgtgctcctg tattagctta cgcgaataca caacaaggca aaacaaattc acaagcaaat aacggcactt tgccacatca acttgaaagc aagcctgatg agacaacaca gcgcttcaat catcttctca aacctacagt gcttgaaggt attcgtgctt caacccttgg caatcgaaat tatatcatca cgatgggaac ttctatccct tgtatcttag aaaccgccat caatagtgat caacaagggt ttgccagttg tatcgtttcc agagatattt tatcagacaa tggtcgcgtt gttcttcttg ataaaggtac ccaaattgtt ggtgaatatc gcgccggttt gaaaaaaggc caaaaacgtc tctttgtgct atggaataga gcaaaaaccc ccaatggtat cattatatcc ttagcttcac cggcaacaga cagtttaggg cgttctggta ttgacggtga tattgataat cattggttag agaggattgg atctgcactt cttgtatccc tcataaaaga tgcaacaagc tatgccaata aacgtttatc aaaaaagcaa gacaaagaag agaatgaaac aatctcttca gggcaaaaca ttgcaaatat tattgtcgaa aattacgcga atattcctcc aactttatcc aaaaaccaag gtgaaatggt gaatgttttt gttgcccgcg atttagattt ttccagcgtt tataaattga aagtcatcga aaacaaaaag cagatcatca atcgggctgt ttcaagaaac ttttacaaga attctgcgat acctttaaaa tga
Details for BatrA.18411.a.B2.GE35764
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/3/2012
EXPECTED MW: 38kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MSTLVTDKKQ PIESSKEASK EGKVIKQTEL FRPAQPAPPS VEPAHRKNAL LPKVELPTPN INQLNSDDPL LEAAQRAPVL AYANTQQGKT NSQANNGTLP HQLESKPDET TQRFNHLLKP TVLEGIRAST LGNRNYIITM GTSIPCILET AINSDQQGFA SCIVSRDILS DNGRVVLLDK GTQIVGEYRA GLKKGQKRLF VLWNRAKTPN GIIISLASPA TDSLGRSGID GDIDNHWLER IGSALLVSLI KDATSYANKR LSKKQDKEEN ETISSGQNIA NIIVENYANI PPTLSKNQGE MVNVFVARDL DFSSVYKLKV IENKKQIINR AVSRNF
Validated NT Sequence
taaaagtttc ttgaaacagc ccgattgatg atctgctttt tgttttcgat gactttcaat ttataaacgc tggaaaaatc taaatcgcgg gcaacaaaaa cattcaccat ttcaccttgg tttttggata aagttggagg aatattcgcg taattttcga caataatatt tgcaatgttt tgccctgaag agattgtttc attctcttct ttgtcttgct tttttgataa acgtttattg gcatagcttg ttgcatcttt tatgagggat acaagaagtg cagatccaat cctctctaac caatgattat caatatcacc gtcaatacca gaacgcccta aactgtctgt tgccggtgaa gctaaggata taatgatacc attgggggtt tttgctctat tccatagcac aaagagacgt ttttggcctt ttttcaaacc ggcgcgatat tcaccaacaa tttgggtacc tttatcaaga agaacaacgc gaccattgtc tgataaaata tctctggaaa cgatacaact ggcaaaccct tgttgatcac tattgatggc ggtttctaag atacaaggga tagaagttcc catcgtgatg atataatttc gattgccaag ggttgaagca cgaatacctt caagcactgt aggtttgaga agatgattga agcgctgtgt tgtctcatca ggcttgcttt caagttgatg tggcaaagtg ccgttatttg cttgtgaatt tgttttgcct tgttgtgtat tcgcgtaagc taatacagga gcacgttgtg ctgcttccaa caggggatca tcagaattta attgattgat gttgggggtt ggtagctcga ctttgggtaa cagagcgttc tttctgtgag ccggttcaac ggagggagga gcaggctggg caggacgaaa aagttctgtt tgtttaataa ctttaccttc ttttgatgct tcttttgaag attcaatcgg ttgttttttg tctgtaacaa gcgttgacat a
Expected Protein Sequence
MAHHHHHHMS TLVTDKKQPI ESSKEASKEG KVIKQTELFR PAQPAPPSVE PAHRKNALLP KVELPTPNIN QLNSDDPLLE AAQRAPVLAY ANTQQGKTNS QANNGTLPHQ LESKPDETTQ RFNHLLKPTV LEGIRASTLG NRNYIITMGT SIPCILETAI NSDQQGFASC IVSRDILSDN GRVVLLDKGT QIVGEYRAGL KKGQKRLFVL WNRAKTPNGI IISLASPATD SLGRSGIDGD IDNHWLERIG SALLVSLIKD ATSYANKRLS KKQDKEENET ISSGQNIANI IVENYANIPP TLSKNQGEMV NVFVARDLDF SSVYKLKVIE NKKQIINRAV SRNFYKNSAI PLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa cgcttgttac agacaaaaaa caaccgattg aatcttcaaa agaagcatca aaagaaggta aagttattaa acaaacagaa ctttttcgtc ctgcccagcc tgctcctccc tccgttgaac cggctcacag aaagaacgct ctgttaccca aagtcgagct accaaccccc aacatcaatc aattaaattc tgatgatccc ctgttggaag cagcacaacg tgctcctgta ttagcttacg cgaatacaca acaaggcaaa acaaattcac aagcaaataa cggcactttg ccacatcaac ttgaaagcaa gcctgatgag acaacacagc gcttcaatca tcttctcaaa cctacagtgc ttgaaggtat tcgtgcttca acccttggca atcgaaatta tatcatcacg atgggaactt ctatcccttg tatcttagaa accgccatca atagtgatca acaagggttt gccagttgta tcgtttccag agatatttta tcagacaatg gtcgcgttgt tcttcttgat aaaggtaccc aaattgttgg tgaatatcgc gccggtttga aaaaaggcca aaaacgtctc tttgtgctat ggaatagagc aaaaaccccc aatggtatca ttatatcctt agcttcaccg gcaacagaca gtttagggcg ttctggtatt gacggtgata ttgataatca ttggttagag aggattggat ctgcacttct tgtatccctc ataaaagatg caacaagcta tgccaataaa cgtttatcaa aaaagcaaga caaagaagag aatgaaacaa tctcttcagg gcaaaacatt gcaaatatta ttgtcgaaaa ttacgcgaat attcctccaa ctttatccaa aaaccaaggt gaaatggtga atgtttttgt tgcccgcgat ttagattttt ccagcgttta taaattgaaa gtcatcgaaa acaaaaagca gatcatcaat cgggctgttt caagaaactt ttacaagaat tctgcgatac ctttaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat